LOCUS       CP103190                4401 bp    DNA     circular BCT 29-AUG-2022
DEFINITION  Bacteroides fragilis strain BFG-57 plasmid unnamed2, complete
            sequence.
ACCESSION   CP103190
VERSION     CP103190.1
DBLINK      BioProject: PRJNA646575
            BioSample: SAMN30323461
KEYWORDS    .
SOURCE      Bacteroides fragilis
  ORGANISM  Bacteroides fragilis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Genome Sequencing of Bacteroides fragilis Group Isolates with
            Nanopore Technology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4401)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2022) LCIM, National Institute of Allergy and
            Infectious Diseases, 9000 Rockville Pike, Bethesda, MD 20892, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 204x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/22/2022 22:21:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,594
            CDSs (total)                      :: 4,499
            Genes (coding)                    :: 4,336
            CDSs (with protein)               :: 4,336
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 163
            CDSs (without protein)            :: 163
            Pseudo Genes (ambiguous residues) :: 0 of 163
            Pseudo Genes (frameshifted)       :: 128 of 163
            Pseudo Genes (incomplete)         :: 34 of 163
            Pseudo Genes (internal stop)      :: 22 of 163
            Pseudo Genes (multiple problems)  :: 17 of 163
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Bacteroides fragilis"
                     /mol_type="genomic DNA"
                     /strain="BFG-57"
                     /host="Homo sapiens"
                     /db_xref="taxon:817"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: Bethesda"
                     /collection_date="1986-07-03"
     gene            1..1026
                     /locus_tag="NXY10_22930"
     CDS             1..1026
                     /locus_tag="NXY10_22930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008657007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UVR36040.1"
                     /translation="MKKKLPITKNKDVVVSWVYTWSKQQDMSIHEQRIVLRILEACQAE
                     LKGVKLKDYAGTKRKFEHGLWDVDAQMHVSDVIFSGRDYNEIIAALDSLAGRFFTYEDD
                     EEWWKCGFISNPKYKKHTGIITFRVSNDLWDVFTKFAKGYREFELNKALALPTGYSLRF
                     YMLMSGQVYPLDISLDNLKDRLGIPADKYKDKNGKDRIDNFEERVLKPAKAALDESCPY
                     TFNYVKVRENPNNKRSKVTGFRFYPVYQPQFRDEELEGKELQAKVTARYQIDSHVYEYL
                     RYSCGFTSEEINRNKETFITAQEKITDLIGELALLNGKSREKNNPKGWIINALKGKIKD
                     K"
                     /name="replication initiation protein"
     gene            complement(1063..1293)
                     /locus_tag="NXY10_22935"
     CDS             complement(1063..1293)
                     /locus_tag="NXY10_22935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005683050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="charged multivesicular body protein 2b-B"
                     /protein_id="UVR36041.1"
                     /translation="MGTTEHEEPRFFFILNKGAKSGGEITHAVLNGSIVSKPAGWDAFH
                     GLALAKEKLSSEEIQQQMKELGVEMEIVPLI"
                     /name="charged multivesicular body protein 2b-B"
     gene            complement(1312..1764)
                     /locus_tag="NXY10_22940"
     CDS             complement(1312..1764)
                     /locus_tag="NXY10_22940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVR36042.1"
                     /translation="METNSHPEAAASTVKQGQYSPRKRKDTAPKVNPQLAIELFFQEMG
                     RIEAYTKRIEDATSRKVQIDGKSLESAENRLKNVLSDFERQGYRMKNGGYVDKRISFYS
                     ILCAVISLLFACFMCYLWTDAAKDRDNYKQYYEYFQEQAREQKGNK"
                     /name="hypothetical protein"
     gene            complement(1721..2515)
                     /locus_tag="NXY10_22945"
     CDS             complement(1721..2515)
                     /locus_tag="NXY10_22945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="UVR36043.1"
                     /translation="MIGKGKSISHGTAALEYDLAKEIDGQTAAIEIARHELYGCTGAEM
                     VQEMKPYHADFPNVKNNCLRFEVSPSIEESATFTDADWAELGNDFMQRMGLANHQYIII
                     RHSGTESKKEQAHLHILANRVSLSGELYRDNWIGKKATEAANAIAKERNFVQSKDIGKA
                     NKAEIKEAMDDVLKKMQGFDLTKFKEELGRRGFKVREARASTGKLNGYYVTARSGTEYK
                     ASEIGKGYTLAHIERTQSKLKYNSMNISHGNKLTPGSGSFHR"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2526..2768)
                     /locus_tag="NXY10_22950"
                     /pseudo=""
     CDS             complement(2526..2768)
                     /locus_tag="NXY10_22950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560590.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2765..3064)
                     /locus_tag="NXY10_22955"
     CDS             complement(2765..3064)
                     /locus_tag="NXY10_22955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVR36036.1"
                     /translation="MEVRRNEKITFRCTRYEKLALAEQAARCSMSTSEYCRSLSLGGRP
                     RERYTEEERQLLRDIAQLKGTLQRLNNYFGGRQYREVFEENQALITELKKILSR"
                     /name="hypothetical protein"
     gene            complement(3258..3455)
                     /locus_tag="NXY10_22960"
     CDS             complement(3258..3455)
                     /locus_tag="NXY10_22960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVR36037.1"
                     /translation="MVEYCVYWLENGEPMHEVFSNLAAAEMYSCAIRGKENVEWVEVSE
                     EEAIDLDELEDMFPDDFCGV"
                     /name="hypothetical protein"
     gene            complement(3466..3741)
                     /locus_tag="NXY10_22965"
     CDS             complement(3466..3741)
                     /locus_tag="NXY10_22965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004519 - endonuclease activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YoeB family toxin"
                     /protein_id="UVR36038.1"
                     /translation="MMYTIRVSDGVDKVIAKWKKSNPNLFKKYKKIYKELLEHPKTGLG
                     HPEALRGGGDITWSRHITAHDRIIYDIYEEVVEVYILEVEGHYNDK"
                     /name="type II toxin-antitoxin system YoeB family toxin"
     gene            complement(3738..3989)
                     /locus_tag="NXY10_22970"
     CDS             complement(3738..3989)
                     /locus_tag="NXY10_22970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVR36039.1"
                     /translation="MEALSVREYRNNLAASFTKADNGEQVLIRRKNEIYALVKVGREDL
                     MITPELQARIDKAREEIKSGKCVTLKSSEDIDAYFDSL"
                     /name="hypothetical protein"
     Typing          complement(2527..2768)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="83.951"
                     /coverage="99.0"
                     /evalue="6.92e-38"
ORIGIN
        1 atgaaaaaga aactacctat taccaaaaac aaagatgttg ttgtttcatg ggtatataca
       61 tggtctaaac agcaagatat gtccatacac gaacaaagaa tagtacttcg tatcttggaa
      121 gcatgccagg cagaattgaa aggggtaaag ctgaaagatt atgcaggtac gaaacgcaaa
      181 tttgagcatg gactttggga tgttgatgca caaatgcatg tctcggacgt tattttttcc
      241 ggacgtgatt acaatgaaat catagccgca ctcgattctt tggcaggacg ttttttcact
      301 tacgaagatg acgaggagtg gtggaagtgc ggatttatat ctaatcccaa atataaaaaa
      361 cacactggca ttataacctt tcgtgtgtct aatgaccttt gggatgtgtt taccaagttc
      421 gccaaggggt acagagagtt tgaattaaac aaggctcttg cgctgcctac cgggtattcc
      481 cttaggtttt acatgctaat gagtgggcaa gtgtaccctt tggatatatc ccttgacaac
      541 ttgaaagacc gccttggcat accagcggac aagtacaagg acaagaacgg aaaagataga
      601 attgacaact tcgaggaaag ggttttaaaa cctgcaaagg ctgcgcttga tgagagttgc
      661 ccatacactt tcaactacgt gaaagtgcgt gaaaacccaa acaacaaacg tagcaaggtg
      721 accggattta ggttttaccc ggtctatcag ccacagttca gggatgaaga actggaaggg
      781 aaagaattgc aggcaaaggt tacagcacga tatcagatag acagccatgt atacgagtat
      841 ctccgttatt cctgcggttt cacatcggaa gagataaacc ggaacaaaga gacattcata
      901 acggcacaag aaaagataac cgaccttatc ggagaactgg ctctcctaaa cggaaaatca
      961 cgagaaaaga acaatccgaa agggtggatt ataaacgccc ttaaagggaa aatcaaggat
     1021 aagtaacagg attacccggc tgaaacactc cgggtaatct tgtcaaatca gaggaacgat
     1081 ttccatttct acaccaagtt ctttcatctg ctgttgtatt tcctcgctgc ttagtttctc
     1141 tttcgccagt gcaagcccat gaaaggcgtc ccagcctgcc ggcttcgaaa cgatactacc
     1201 gtttaggacg gcatgggtga tttcgccgcc ggacttcgca cccttgttca gtatgaagaa
     1261 gaaccggggt tcttcgtgtt ccgttgttcc cataattcgt aaagttagag tttatttgtt
     1321 tcccttttgt tcccttgcct gctcttggaa gtattcataa tactgcttgt agttgtcccg
     1381 gtctttggct gcatccgtcc aaagatagca catgaaacaa gcgaacaata gggagataac
     1441 agcacaaagg attgagtaga acgaaatccg tttgtccaca taaccgccgt ttttcattct
     1501 gtacccctgc cgttcaaagt ccgacaatac gttttttagg cgattttcgg cactttcgag
     1561 gcttttcccg tctatttgta cctttcggga tgttgcatcc tctatgcgct ttgtataggc
     1621 ttcaatacgt cccatttctt gaaaaaacag ttctatcgca agctgcgggt taacctttgg
     1681 tgctgtgtct tttcttttcc gggggctgta ctgtccctgc ttaacggtgg aagctgccgc
     1741 ttccgggtgt gagtttgttt ccatgagata tgttcattga gttatatttc agtttgcttt
     1801 gtgtccgctc gatatgggcg agagtgtagc ctttccctat ctcgctcgcc ttatactccg
     1861 taccgcttcg ggctgtgacg tagtagccgt taagttttcc ggtgcttgcc ctggcttccc
     1921 gaactttaaa acctctccta ccaagttctt ccttgaattt agtcaagtca aagccctgca
     1981 tcttcttcaa gacgtcgtcc atggcttcct tgatttccgc tttgttggct ttgccgatgt
     2041 ccttcgactg tacaaagttc cgttccttgg ctatggcgtt ggcggcttcc gttgcctttt
     2101 tccctatcca gttatcccgg tacagttccc cggaaaggga aacccggttt gccagtatat
     2161 gcaggtgggc ttgttctttt ttgctctccg taccgctgtg ccggatgatg atgtactggt
     2221 ggttcgccag ccccatgcgc tgcatgaagt cgttaccaag ttccgcccag tccgcatccg
     2281 taaaggtggc gctttcctct atcgaaggac tgacctcgaa acgtaggcaa ttgtttttta
     2341 cgttcggaaa atcagcgtgg tatggtttca tttcctgtac catttccgca cccgtacacc
     2401 cgtaaagttc atgccgggct atctcgatgg ctgctgtctg tccgtctatc tctttggcaa
     2461 ggtcatactc cagtgcagcc gtaccatgcg atatgctctt tcctttccct atcatcgtga
     2521 aagtatgcat ccgtaaaggt ggcgctttcc tctatcgaag gactgacctc gaaacgtagg
     2581 caattgtttt ttacgttcgg aaaatcagcg tggtatggtt tcatttcctg taccatttcc
     2641 gcacccgtac acccgtaaag ttcatgccgg gctatctcga tggctgctgt ctgtccgtct
     2701 atctctttgg caaggtcata ctccagtgca gccgtaccat gcgatatgct ctttcctttc
     2761 cctatcatcg tgaaagtatt tttttaagtt cggtgattag tgcctgattc tcttcaaata
     2821 cctcccggta ctggcgacca ccgaagtagt tgttcagccg ttggagcgtt cctttcaact
     2881 gggctatgtc ccggagaagc tgccgttcct cctcggtgta cctttccctt gggcgacctc
     2941 caagggacaa actccggcaa tactccgatg tgctcatgct acatcgggct gcctgctccg
     3001 caagtgccag cttttcatac cgggtacagc ggaatgtaat cttttcgttc cgtcttactt
     3061 ccatgtcaca aaggtaatct taaaatcttt ttgagcaaag cgagaaaagc aagaattgtc
     3121 cgacaaggac acttcttgct atatatacac atgccgcatg ggaatacatc catgctaacc
     3181 gcccggcaaa aagccccggc gtgttagaaa atccccccac agcctaacaa tttctacccc
     3241 atttctaccc aaggggatta cacgccgcag aagtcatccg ggaacatgtc ttccagttcg
     3301 tccaaatcaa tggcttcttc ttcggacacc tccacccatt caacgttttc tttccctctt
     3361 atcgcacatg agtacatctc ggcggcggca aggttagaga acacctcgtg cataggctcg
     3421 ccgttctcta accagtaaac acaatattcg accataacag ttttatcatt tatcgttgta
     3481 atgcccctct acctccaaaa tatagacctc taccacttcc tcgtaaatgt catagattat
     3541 ccggtcatgg gcggtgatgt gtcgagacca tgtaatatcc ccaccgcccc ttaacgcttc
     3601 gggatgccca agcccagtct tagggtgctc taacaattct ttgtagattt tcttatactt
     3661 cttgaacaaa ttggggtttg atttcttcca tttggcaatc accttatcca ccccgtcgga
     3721 aaccctaatt gtgtacatca tagactgtcg aaataagcgt caatatcctc actgcttttg
     3781 agggtaacac actttccgga tttgatttct tcccttgcct tgtcaatcct tgcttgcagc
     3841 tccggggtta tcatcaaatc ttcacgacca acttttacca aagcgtaaat ctcgtttttc
     3901 ctgcgaatta gtacctgctc gccattgtca gctttggtga aagacgctgc gaggttgttt
     3961 cggtattctc ttactgataa tgcttccata tctgtttgct tttaaattca gcacaaagat
     4021 agctatattt caataaaata caaacttttt gtacacaaat gtgtacacga cataaaaagc
     4081 cgctcacagt cctaccgccc gttggttggt tttgctttgc tctttttccc tattgttttt
     4141 ctttttccga cagtaaatca gccatttaac tctataaatt tcccttgtct gttataagca
     4201 tcgtctgtaa tatgctctat cacttcttcg caaataccca tgaataaagg gattaaagca
     4261 tgagtttccc tacattttgg gagttttaat cctacacttt gggagttttt cacctcacta
     4321 ttccctacac tttgggagtt ttacccttct ttttttgttt caatgtaggt tttatgtata
     4381 tttgcagcaa atcaatcgct t
//
