LOCUS       CP103171                2700 bp    DNA     circular BCT 29-AUG-2022
DEFINITION  Bacteroides fragilis strain BFG-317 plasmid unnamed2, complete
            sequence.
ACCESSION   CP103171
VERSION     CP103171.1
DBLINK      BioProject: PRJNA646575
            BioSample: SAMN30323282
KEYWORDS    .
SOURCE      Bacteroides fragilis
  ORGANISM  Bacteroides fragilis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Genome Sequencing of Bacteroides fragilis Group Isolates with
            Nanopore Technology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2022) LCIM, National Institute of Allergy and
            Infectious Diseases, 9000 Rockville Pike, Bethesda, MD 20892, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 253x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/22/2022 19:21:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,569
            CDSs (total)                      :: 4,476
            Genes (coding)                    :: 4,233
            CDSs (with protein)               :: 4,233
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 243
            CDSs (without protein)            :: 243
            Pseudo Genes (ambiguous residues) :: 0 of 243
            Pseudo Genes (frameshifted)       :: 198 of 243
            Pseudo Genes (incomplete)         :: 61 of 243
            Pseudo Genes (internal stop)      :: 12 of 243
            Pseudo Genes (multiple problems)  :: 24 of 243
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Bacteroides fragilis"
                     /mol_type="genomic DNA"
                     /strain="BFG-317"
                     /host="Homo sapiens"
                     /db_xref="taxon:817"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: Bethesda"
                     /collection_date="1992-08-19"
     gene            1..1104
                     /locus_tag="NXX17_22840"
     CDS             1..1104
                     /locus_tag="NXX17_22840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="UVR20352.1"
                     /translation="MGATSIHVQAVKPGSEIHNFREKELDYVRPELSHLNESWVGDSIS
                     HRLESAKQRYLDTVGQKMQAKAAPIREGVIVIKQETTMQELQQFATVCKERFGIEAFQI
                     HIHKDEGYMNAKQWTPNLHAHVVFDWTQPNGKSVRLSRDDMAELQTIASETLGMERGVS
                     SDRKHLSAMQYKTECAKEQLQELSNDISSALDKHKDVQNQLLQLQKELRSIETKKNVQK
                     LISKASEKFYGLIGKTVNDREKDALKAKVKALEGENEQLSDRLGKAILEKEQNGTKAFK
                     AENDKEYYRQQMDNARTTSNRLRTENQELKTETKELKKELGKMKDLFNSEQLEALRHHF
                     PNISKAMEEGKDLLKQITRSRGFGMGM"
                     /name="mobilization protein"
     gene            1493..2083
                     /locus_tag="NXX17_22845"
     CDS             1493..2083
                     /locus_tag="NXX17_22845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_process:
                     GO:0006260 - DNA replication [Evidence IEA]; GO_process:
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="UVR20351.1"
                     /translation="MENKKAVKLTDFQKNEENPFMKQAIEGIENHVVKKYKSNSGGDKR
                     AVVALADTETGEVFKTSFIRQIEVDEEQFTKLYLSNFAAFFDLSQAAIRVFGYFMTCMK
                     PKNDLIIFNRKKCLEYTKYKTDKAVYKGLAELVKAEIIARGPADNLWFINPLIVFNGDR
                     VTFAKTYVRKKTLAAQKKEEAEKRQLSLGFDEQ"
                     /name="replication/maintenance protein RepL"
     Typing          1461..2073
                     /name="rep_cluster_1868"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.701"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2..1101
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.275"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgggagcaa caagtattca tgtacaagca gtgaagccgg ggagtgagat tcacaacttt
       61 agggaaaaag agttggacta tgttcgtccc gaacttagtc atttgaatga aagctgggtt
      121 ggagatagca tctcccatcg gctggaaagt gcgaaacaaa gatatctcga tacggttggg
      181 cagaagatgc aggctaaagc cgcacccata cgagagggag taatagtaat caaacaagaa
      241 accaccatgc aggaactcca gcagtttgcc acggtctgca aagaacgttt cggtatcgaa
      301 gcatttcaaa tccatataca caaggacgaa ggatacatga acgcaaagca atggacacct
      361 aaccttcatg cccatgtagt tttcgattgg acgcagccga atgggaaaag tgtgcgttta
      421 tcgcgtgatg acatggcaga actccaaacc atagcatctg aaacactggg catggaacgt
      481 ggcgtttctt ctgaccgcaa acacttatcg gctatgcagt acaagaccga atgtgcgaaa
      541 gaacagcttc aggaactatc aaacgatata tccagtgcat tagacaaaca caaggacgta
      601 caaaaccagc ttcttcagct tcagaaagaa ctacgttcca ttgaaacaaa gaagaacgtc
      661 caaaaactca tttctaaggc ttcagagaag ttttacggct tgattggaaa aacagtcaac
      721 gatagggaaa aggatgcctt aaaagccaaa gtaaaggctt tagaggggga aaatgaacag
      781 ctatccgata gactgggaaa ggctatactt gaaaaagagc aaaacggcac aaaggcattc
      841 aaagccgaga atgacaagga gtattataga caacaaatgg acaacgcaag gacaaccagt
      901 aaccgattaa ggacggagaa ccaagaacta aagaccgaaa ccaaggaatt gaaaaaagaa
      961 cttggtaaga tgaaagactt gttcaattcc gaacaactgg aggctttaag gcatcatttc
     1021 ccaaacatat caaaagctat ggaagagggg aaagacctac tcaagcaaat caccagaagc
     1081 agaggtttcg gtatgggtat gtaacccccc ccgcccccaa agggggagcg accaaacggc
     1141 agcctctctc aatggagtgt tacgtcgttc ggattcggaa gaaattttgc agggttcgga
     1201 aaacggttgt gtgcttattc taaaaaaagt ttatggtgtg gcgctcgtcg ggacgggtgg
     1261 tcccgcccct tgccgctggg cgctccccga ccgatgattt ttaagtgact gattttgtgc
     1321 tgttttgggg gtatattaag aatagaagaa atagaataag ttaagtactt gatacacaat
     1381 atagggcatt ttccatattg gaaattctca ttttccaatc tagaaaatac tgatttcatg
     1441 gaaaataata gtttactttg tggagaataa tatttcccaa aaacacatca aaatggaaaa
     1501 taaaaaagca gttaagttaa ccgattttca aaagaacgaa gaaaatcctt ttatgaaaca
     1561 agctatagaa ggtattgaaa atcatgttgt taaaaagtat aagagtaata gtggtggcga
     1621 taagagagcc gtagtagctt tagccgacac tgaaactgga gaagtgttta agacttcgtt
     1681 tatccgtcaa atagaagtag atgaagaaca attcactaaa ttgtatcttt ctaactttgc
     1741 tgcattcttt gacctatcac aagcagctat tcgggttttt ggttacttta tgacctgcat
     1801 gaaacccaaa aatgatttaa tcatcttcaa tagaaaaaaa tgcctagaat ataccaaata
     1861 caaaacagac aaagccgttt ataaaggact tgcagaactt gtaaaagctg aaatcatagc
     1921 ccgaggacca gccgataatc tttggtttat taatcctctg atagtattca atggtgaccg
     1981 agtgacattt gctaaaacat acgttcggaa aaagacttta gctgcccaaa agaaagaaga
     2041 agcagagaaa cgacaattat cacttggctt tgatgaacag taacactcca ttgagtgaag
     2101 ctgccgtttg gtcgctcccc ctttgggggc ggggggggat agataaagtt cctctatgta
     2161 aagttataat gggggatgaa aggcaaggtt cgctaacctt accccgattg tttatgcttc
     2221 ccagcattca atctatcccc gtttatgggt ttctctttca ccctacaaag ataacctcat
     2281 gggggaaaaa tgtccaagaa tatggggtaa aaactatcaa gtcggtagaa aataagtatc
     2341 tttgaagtac atttttagta gaggtactgg tatgcctaga cgaagcagat aggcaaaaat
     2401 aaagccgctg gagatgtcgg atgtccgtca attaactcca gcggcttgta ttttggtaac
     2461 tctcccgtat cggtcggcta ccgtttggtc agcaacaaag gtagtacttt attgtcgcaa
     2521 atccaaatgt tttccccttt atttgtgcat ttacagaaca tttgctaact tcgcacctat
     2581 gcagtatggt gatagcacac tagcacccta aagggtgcgt gtgtaagggg acaagccccc
     2641 ttaaccccct gtcagccggc tgtcaccggc tgttatcgat tgtcacaaaa atgttaactt
//
