LOCUS       CP103170                4285 bp    DNA     circular BCT 29-AUG-2022
DEFINITION  Bacteroides fragilis strain BFG-317 plasmid unnamed1, complete
            sequence.
ACCESSION   CP103170
VERSION     CP103170.1
DBLINK      BioProject: PRJNA646575
            BioSample: SAMN30323282
KEYWORDS    .
SOURCE      Bacteroides fragilis
  ORGANISM  Bacteroides fragilis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Genome Sequencing of Bacteroides fragilis Group Isolates with
            Nanopore Technology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4285)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2022) LCIM, National Institute of Allergy and
            Infectious Diseases, 9000 Rockville Pike, Bethesda, MD 20892, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 253x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/22/2022 19:21:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,569
            CDSs (total)                      :: 4,476
            Genes (coding)                    :: 4,233
            CDSs (with protein)               :: 4,233
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 243
            CDSs (without protein)            :: 243
            Pseudo Genes (ambiguous residues) :: 0 of 243
            Pseudo Genes (frameshifted)       :: 198 of 243
            Pseudo Genes (incomplete)         :: 61 of 243
            Pseudo Genes (internal stop)      :: 12 of 243
            Pseudo Genes (multiple problems)  :: 24 of 243
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Bacteroides fragilis"
                     /mol_type="genomic DNA"
                     /strain="BFG-317"
                     /host="Homo sapiens"
                     /db_xref="taxon:817"
                     /plasmid="unnamed1"
                     /geo_loc_name="USA: Bethesda"
                     /collection_date="1992-08-19"
     gene            join(4038..4285,1..4)
                     /locus_tag="NXX17_22800"
     CDS             join(4038..4285,1..4)
                     /locus_tag="NXX17_22800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVR20346.1"
                     /translation="MEALSVREYRNNLAASFTKADNGEQVLIRRKNEIYALVKVGREDL
                     MITPELQARIDKAREEIKSGKCVTLKSSEDIDAYFDSL"
                     /name="hypothetical protein"
     gene            1..276
                     /locus_tag="NXX17_22805"
     CDS             1..276
                     /locus_tag="NXX17_22805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004519 - endonuclease activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YoeB family toxin"
                     /protein_id="UVR20347.1"
                     /translation="MMYTIRVSDGVDKVIAKWKKSNPNLFKKYKKIYKELLEHPKTGLG
                     HPEALRGGGDITWSRHITAHDRIIYDIYEEVVEVYILEVEGHYNDK"
                     /name="type II toxin-antitoxin system YoeB family toxin"
     gene            287..484
                     /locus_tag="NXX17_22810"
     CDS             287..484
                     /locus_tag="NXX17_22810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVR20348.1"
                     /translation="MVEYCVYWLENGEPMHEVFSNLAAAEMYSCAIRGKENVEWVEVSE
                     EEAIDLDELKDMFPDDFCGV"
                     /name="hypothetical protein"
     gene            962..1756
                     /locus_tag="NXX17_22815"
     CDS             962..1756
                     /locus_tag="NXX17_22815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="UVR20349.1"
                     /translation="MIGKGKSISHGVAALEYDLAKEINGQAVATEIARHELYGCTGAEM
                     VQEMKPYHIDFPNVKNNCLRFEVSPSIEESATFTDADWAELGNDFMQRMGLANHQYIII
                     RHSGTESKKEQAHLHILANRVSLSGELYRDNWIGKKATEAANAIAKERNFVQSQDIGKV
                     NKAEIKEAMDGVLKKMQGFDFTKFKEELGKRGFKVREARASTGKLNGYYVTARSGTEYK
                     ASEIGKGYTLAHIERTQSKLKCNSMNISHGNKLTPGSGSFQR"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1713..2165
                     /locus_tag="NXX17_22820"
     CDS             1713..2165
                     /locus_tag="NXX17_22820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVR20350.1"
                     /translation="METNSHPEAAASNVKQGQYSPRKRKDTTPRVNPQLAVELVYQELK
                     RVEVYTKRIEDATARKVQIDGKSLESAENRLKNVLADFERQGYRMKNGGYVDKRISFYS
                     ILCAVISLLFACFMCYLWTDAAKDRDNYKQYYEYYQEQAREQKGNK"
                     /name="hypothetical protein"
     gene            2185..2415
                     /locus_tag="NXX17_22825"
     CDS             2185..2415
                     /locus_tag="NXX17_22825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005683050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="charged multivesicular body protein 2b-B"
                     /protein_id="UVR20343.1"
                     /translation="MGTAEHEEPRFFFILNKGAKSGGEITHAVLNGSIVSKPAGWDAFH
                     GLVLAKEKLSSEEIQRQMKELGVEMEIVPLI"
                     /name="charged multivesicular body protein 2b-B"
     gene            complement(2716..3366)
                     /locus_tag="NXX17_22830"
     CDS             complement(2716..3366)
                     /locus_tag="NXX17_22830"
                     /inference="COORDINATES: protein motif:HMM:NF013235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UVR20344.1"
                     /translation="MTTAKYDFSVYEKRILYRIVEMAQGELQGVRFATDCKKIEHTLFD
                     TVHITMPIASLLNGESDENYTRVKQALLSLQKKIFTYEDEKEWISISIIALPKIKKRSS
                     VVSFQIDSHIWDCCLDFSKGFRKYELATAMKFKSVYSMRFYELLSGQKTKLIYPLEQLK
                     EMFKVQDKYAKTNDFVRKVIEPAKSLYIRMVRKQGREKNCKFQLLSNIQARAS"
                     /name="replication initiation protein"
     gene            complement(3389..3574)
                     /locus_tag="NXX17_22835"
     CDS             complement(3389..3574)
                     /locus_tag="NXX17_22835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVR20345.1"
                     /translation="MKKIIVHFKKEEFERVKNAISILAKFSEIYAPHIDKANSILSFRV
                     DDENETTEEQQCNSRQ"
                     /name="hypothetical protein"
     Typing          complement(2455..3438)
                     /name="rep_cluster_1019"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.01"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgatgtaca caattagggt ttccgacggg gtggataagg tgattgccaa atggaagaaa
       61 tcaaacccca atttgttcaa gaagtataag aaaatctaca aagaattgtt agagcaccct
      121 aagactgggc ttgggcatcc cgaagcgtta aggggcggtg gggatattac atggtctcga
      181 cacattaccg cccatgaccg gataatctat gacatttacg aggaagtggt agaggtctac
      241 attttggagg tagaggggca ttacaacgat aaatgataaa actgttatgg tcgaatattg
      301 tgtttactgg ttagagaacg gcgagcctat gcacgaggtg ttctctaacc ttgccgccgc
      361 cgagatgtac tcatgtgcga taagagggaa agaaaacgtt gaatgggtgg aggtgtccga
      421 agaagaagcc attgatttgg acgaactgaa agacatgttc ccggatgact tctgcggcgt
      481 gtaatcccct tgggtagaaa ttgttaggct gtgggggatt ttctaacatg cctgggcttt
      541 ttgccgggcg gttggcgtgg atgtattccc atgcggcatg tgtatatata gcaagaagtg
      601 tccttgtcgg acaattcttg cttttctcgc tttgctcaaa aagattttaa gattaccttt
      661 gtggcatgga actaagacgg aacgaaaaga ttacattccg atgtaccgaa cttgaaaagg
      721 acgcacttgc ggagcaggca gcccggtgca gtctaagcgt atcggaatac tgccggagtt
      781 tgtcccttgg ggggcgccca agggagaggt acaccgagga ggaacggcag cttctccggg
      841 acatagccca gttgaaagga acgctccaac ggctgaacaa ctacttcggt ggtcgccagt
      901 accgggaagt gttcgaggag aaccgggcac taattacaga gctcaaaaaa atactctcac
      961 gatgataggg aaagggaaaa gcatatcaca tggtgtggcg gcattggagt acgacctcgc
     1021 caaagagata aacgggcagg cagtagccac cgagatagcc cggcatgaac tttatgggtg
     1081 tactggtgcg gaaatggtgc aggaaatgaa accgtatcac attgattttc cgaacgtaaa
     1141 aaacaactgc ctacgtttcg aggtcagccc ctcgatagag gaaagcgcca cctttacgga
     1201 tgcggactgg gcggaacttg gtaacgactt catgcagcgc atggggttgg cgaaccacca
     1261 gtacatcatc atccggcaca gcggtacgga gagcaagaaa gagcaagccc acctgcacat
     1321 actggcaaac cgggtttccc tttccgggga actgtaccgg gacaactgga tagggaaaaa
     1381 ggcaacggaa gccgccaatg ccatagccaa ggaacggaac tttgtacagt cgcaggacat
     1441 tggcaaagtc aacaaagcgg aaatcaagga agccatggac ggcgtattga agaagatgca
     1501 gggctttgac tttaccaaat tcaaggaaga acttggtaag agaggtttta aagtccggga
     1561 agccagggca agcaccggga aacttaacgg ctactatgtc acagcccgaa gcggtacgga
     1621 gtataaggca agcgagatag ggaaaggcta cactctcgcc catatcgagc ggacacaaag
     1681 caaactgaag tgcaactcaa tgaacatatc tcatggaaac aaactcacac ccggaagcgg
     1741 cagcttccaa cgttaagcag ggacagtaca gcccccggaa aagaaaagac acaacgccaa
     1801 gggtaaaccc gcagcttgcg gtagaactgg tgtatcaaga actaaagcgt gttgaagtct
     1861 atacgaagcg catagaggac gcaacagccc gaaaggtaca aatagacggg aaaagccttg
     1921 aaagtgccga aaatcgccta aaaaacgtat tggcggactt tgaacggcag gggtatagaa
     1981 tgaaaaacgg cggttatgtg gacaaacgaa tttcgttcta ctcaatcctt tgtgctgtta
     2041 tctccctatt gttcgcttgt ttcatgtgct atctttggac ggatgcagcc aaagaccggg
     2101 acaactacaa gcagtattat gaatactacc aagagcaggc aagggaacaa aaggggaata
     2161 aataaacatc taactttacg aattatggga acagcggaac acgaagaacc ccggttcttc
     2221 ttcatactga acaagggtgc gaagtccggc ggcgaaatca cccatgccgt cctaaacggt
     2281 agtatcgttt cgaagccggc aggctgggac gcctttcatg ggcttgtact ggcgaaagag
     2341 aaactaagca gcgaggaaat acagcggcag atgaaagagc ttggtgtaga aatggaaatc
     2401 gttcctctga tttgacaaga ttaccccggt atatttcagc cggggtaatc ttgtcactta
     2461 tccttgattt tccctttaag ggcatttata atccaccctt ttggatttgt ttttgtcctt
     2521 gattttcctc taagtatggc gagttctgtc atgatgtctg gaagttccat ttgtgctgtt
     2581 acaaataaat cccggttgtt tttgatttct ttatcggaga actccaaaga tgtttttaag
     2641 taacttatca cttgcctacc taaatcccat gaaagcccgg tttgcttttg tagctctttt
     2701 ttgtatagct ctgcatcacg atgctctggc ttgaatattg gatagaagtt gaaacttaca
     2761 atttttttcc cttccttgtt tgcggaccat tcgaatgtat aaggattttg caggttcaat
     2821 tactttccgg acaaaatcgt ttgtctttgc gtatttatct tgtactttaa acatttcttt
     2881 gagctgttca agtggataga ttagttttgt tttctgaccg cttaaaagtt cgtagaaccg
     2941 catagaatat acactcttaa acttcatcgc agtggctaat tcgtatttgc gaaacccttt
     3001 tgaaaaatct aagcagcagt cccatatgtg tgagtctatt tggaatgata caacagatga
     3061 acgcttcttt atctttggca atgcaataat agaaatgctt atccattctt tttcatcctc
     3121 ataggtaaag attttctttt gtagagataa cagtgcttgt tttacacgtg tgtagttttc
     3181 atcactttct ccatttagca atgaagcaat aggcattgtg atgtgaacgg tatcaaaaag
     3241 tgtgtgttct atttttttac aatcagttgc aaatctaact ccttggagtt caccttgagc
     3301 catttctaca attctataaa gtatgcgctt ctcataaacg gaaaagtcat acttggctgt
     3361 cgtcataata tagctttgta tttggtcttt attgacgact gttacactgt tgttcttctg
     3421 ttgtttcgtt ttcatcatct acacgaaagg ataaaatact gttggcttta tctatgtgag
     3481 gagcgtatat ttcagaaaat tttgcgagga tagaaattgc gtttttaacc ctttcaaatt
     3541 cttctttctt aaaatgaact atgattttct tcatccttta ttgattatta gtatgttgcg
     3601 caaatataat aagtaattac gacttgtgca aatgaaaatc gtagatgatg caaaataaca
     3661 ttcaaaaagt cgtatttgga agggaaaata acattcaaaa agtcgtagta atgtgttaaa
     3721 tagcattcaa aaagtcgtag taatgtgtta aatagattgt gtaagttatt ggatttcagc
     3781 ataattttta gttaaaaaaa aagctgataa gcatataaac aagttatgct ttataacaag
     3841 tcattgattt tttacggata agtggttgat ttactgtcgg aaaaagaaaa acaatagggg
     3901 aaaaaagcaa agcaaaacca accaacgggc ggtaggactg tgaacggctt tttatggcgt
     3961 gtacacattt gtgtgcaaaa agtttgtatt ttattgaaat atagctatct ttgtactgaa
     4021 tttaaaagca aacagatatg gaagcattat cagtaagaga ataccgtaac aacctcgcag
     4081 cgtctttcac caaagctgac aatggcgagc aggtactaat tcgcaggaaa aacgagattt
     4141 acgctttggt aaaagttggt cgtgaagatt tgatgataac cccggaactg caagcaagga
     4201 ttgacaaggc aagggaagaa atcaaatccg gaaagtgtgt taccctcaaa agcagtgagg
     4261 atattgatgc ttatttcgac agtct
//
