LOCUS       CP103161                4087 bp    DNA     circular BCT 29-AUG-2022
DEFINITION  Bacteroides fragilis strain BFG-481 plasmid unnamed3, complete
            sequence.
ACCESSION   CP103161
VERSION     CP103161.1
DBLINK      BioProject: PRJNA646575
            BioSample: SAMN30323385
KEYWORDS    .
SOURCE      Bacteroides fragilis
  ORGANISM  Bacteroides fragilis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Genome Sequencing of Bacteroides fragilis Group Isolates with
            Nanopore Technology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2022) LCIM, National Institute of Allergy and
            Infectious Diseases, 9000 Rockville Pike, Bethesda, MD 20892, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 486x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/22/2022 19:32:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,520
            CDSs (total)                      :: 4,427
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 236 of 297
            Pseudo Genes (incomplete)         :: 68 of 297
            Pseudo Genes (internal stop)      :: 15 of 297
            Pseudo Genes (multiple problems)  :: 19 of 297
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Bacteroides fragilis"
                     /mol_type="genomic DNA"
                     /strain="BFG-481"
                     /host="Homo sapiens"
                     /db_xref="taxon:817"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA: Bethesda"
                     /collection_date="2003-04-12"
     gene            1..1026
                     /locus_tag="NXY37_22570"
     CDS             1..1026
                     /locus_tag="NXY37_22570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008657007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UVQ90444.1"
                     /translation="MKKKLPITKNKDVVVSWVYTWSKQQDMSIHEQRIVLRILEACQAE
                     LKGVKLKDYAGTKRKFEHGLWDVDAQMHVSDVIFSGRDYNEIIAALDSLAGRFFTYEDD
                     EEWWKCGFISNPKYKKRTGIITFRVSNDLWDVFTKFAKGYREFELNKALALPTGYSLRF
                     YMLMSGQVYPLDISLENLKDRLGIPADKYKDKNGKDRIDHFEERVLKPAKAALDESCPY
                     TFNYVKVRENPNNKRSKVTGFRFYPVYQPQFRDEELEGKELQAKVTARYQIDSHVYEYL
                     RYSCGFTSEEINRNKETFITAQEKITDLIGELALLNGKSREKNNPKGWIINALKGKIKD
                     K"
                     /name="replication initiation protein"
     gene            complement(1063..1293)
                     /locus_tag="NXY37_22575"
     CDS             complement(1063..1293)
                     /locus_tag="NXY37_22575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005683050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="charged multivesicular body protein 2b-B"
                     /protein_id="UVQ90445.1"
                     /translation="MGTTEHEEPRFFFILNKGAKSGGEITHAVLNGSIVSKPAGWDAFH
                     GLALAREKLSSEEIQQQMKELGVEMEIVPLI"
                     /name="charged multivesicular body protein 2b-B"
     gene            complement(1313..1765)
                     /locus_tag="NXY37_22580"
     CDS             complement(1313..1765)
                     /locus_tag="NXY37_22580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVQ90446.1"
                     /translation="METNSHPEAAASNVKQGQYSPRKRKDTTPRVNPQLAVELVYQELK
                     RVEVYTKRIEDATARKVQIDGKSLESAENRLKNVLADFERQGYRMKNGGYVDKRISFYS
                     ILCAVISLLFACFMCYLWTDAAKDRDNYKQYYEYYQEQAREQKGNK"
                     /name="hypothetical protein"
     gene            complement(1722..2516)
                     /locus_tag="NXY37_22585"
     CDS             complement(1722..2516)
                     /locus_tag="NXY37_22585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="UVQ90447.1"
                     /translation="MIGKGKSISHGVAALEYDLAKEINGQAVATEIARHELYGCTGAEM
                     VQEMKPYHIDFPNVKNNCLRFEVSPSIEESATFTDADWAELGNDFMQRMGLANHQYIII
                     RHSGTESKKEQAHLHILANRVSLSGELYRDNWIGKKATEAANAIAKERNFVQSQDIGKV
                     NKAEIKEAMDGVLKKMQGFDFTKFKEELGKRGFKVREARASTGKLNGYYVTARSGTEYK
                     ASEIGKGYTLAHIERTQSKLKCNSMNISHGNKLTPGSGSFQR"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2944..3141)
                     /locus_tag="NXY37_22590"
     CDS             complement(2944..3141)
                     /locus_tag="NXY37_22590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVQ90441.1"
                     /translation="MVEYCVYWLENGEPMHEVFSSLAAAEMYSCAIRGKENIEWVEVSE
                     EETIDLDELEDMFPDDFCGV"
                     /name="hypothetical protein"
     gene            complement(3152..3427)
                     /locus_tag="NXY37_22595"
     CDS             complement(3152..3427)
                     /locus_tag="NXY37_22595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004519 - endonuclease activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YoeB family toxin"
                     /protein_id="UVQ90442.1"
                     /translation="MMYTIRVSDGVDKVIAKWKKSNPNLFKKYKKIYKELLEHPKTGLG
                     HPEALRGGGDITWSRHITAHDRIIYDIYEEVVEVYILEVEGHYNDK"
                     /name="type II toxin-antitoxin system YoeB family toxin"
     gene            complement(3424..3675)
                     /locus_tag="NXY37_22600"
     CDS             complement(3424..3675)
                     /locus_tag="NXY37_22600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVQ90443.1"
                     /translation="MEALSVREYRNNLAASFTKADNGEQVLIRRKNEIYALVKVGREDL
                     MITPELQARIDKAREEIKSGKCVTLKSSEDIDAYFDSL"
                     /name="hypothetical protein"
ORIGIN
        1 atgaaaaaga aactacctat taccaagaat aaagatgttg ttgtttcatg ggtatataca
       61 tggtcaaaac agcaagacat gtccatacac gaacaaagaa tagttcttcg catactggaa
      121 gcgtgccaag ctgaactaaa gggagtaaaa ctgaaagatt atgcaggcac gaaacgcaag
      181 tttgagcatg gactttggga tgttgatgca caaatgcatg tatctgacgt tattttttcc
      241 ggacgtgatt acaatgaaat catagccgca ctcgattctt tggcaggacg tttttttact
      301 tacgaagatg acgaggaatg gtggaagtgc ggatttatat ctaacccaaa atataaaaaa
      361 cgcactggca ttataacctt tcgtgtgtct aacgaccttt gggatgtgtt taccaagttc
      421 gccaaggggt acagagaatt tgagttaaac aaggctcttg cgctacctac tgggtattcc
      481 cttaggtttt acatgttaat gagtgggcag gtgtaccctt tggatatatc ccttgaaaac
      541 ttgaaagacc gtcttggcat acctgcggac aaatacaagg acaagaacgg aaaagacaga
      601 atagatcatt ttgaggaaag agttttgaaa ccagcaaagg ctgcgcttga tgagagctgc
      661 ccatacactt tcaactacgt gaaagtgcgt gaaaacccaa acaacaaacg tagcaaggtg
      721 accgggttta ggttctaccc ggtctatcaa ccacagttca gagatgaaga actggaaggg
      781 aaagaattgc aagcaaaggt tacagcacga tatcagatag acagccatgt gtacgagtac
      841 ctccgttatt cctgcggctt cacatcggaa gagataaacc ggaacaaaga gacattcata
      901 acggcacaag aaaagataac cgaccttatc ggagaactgg ctctcctaaa cggaaaatca
      961 cgagaaaaga acaatccgaa agggtggatt ataaacgccc ttaaagggaa aatcaaggat
     1021 aagtaacaag attacccggc tgaaacactc cgggtaatct tgtcaaatca gaggaacgat
     1081 ttccatttct acaccaagtt ctttcatctg ctgttgtatt tcctcgctgc ttagtttctc
     1141 tctcgccagt gcaagcccat gaaaggcgtc ccagcctgcc ggcttcgaaa cgatactacc
     1201 gtttaggacg gcatgggtga tttcgccgcc ggacttcgca cccttgttca gtatgaagaa
     1261 gaaccggggt tcttcgtgtt ccgttgttcc cataattcgt aaagttagat gtttatttat
     1321 tccccttttg ttcccttgcc tgctcttggt agtattcata atactgcttg tagttgtccc
     1381 ggtctttggc tgcatccgtc caaagatagc acatgaaaca agcgaacaat agggagataa
     1441 cagcacaaag gattgagtag aacgaaattc gtttgtccac ataaccgccg tttttcattc
     1501 tgtacccctg ccgttcaaag tccgccaaca cgttttttag gcgattttcg gcactttcaa
     1561 ggcttttccc gtctatttgt acctttcggg ctgttgcgtc ctctatgcgc ttcgtataga
     1621 cttcaacacg ctttagttct tgatacacca gttctaccgc aagctgcggg tttacccttg
     1681 gcgttgtatc ttttcttttc cgggggctgt actgtccctg cttaacgttg gaagctgccg
     1741 cttccgggtg tgagtttgtt tccatgagat atgttcattg agttgcactt cagtttgctt
     1801 tgtgtccgct cgatatgggc gagagtgtag cctttcccta tctcgcttgc cttatactcc
     1861 gtaccgcttc gggctgtgac atagtagccg ttaagtttcc cggtgcttgc cctggcttcc
     1921 cggactttaa aacctctctt accaagttct tccttgaatt tggtaaagtc aaagccctgc
     1981 atcttcttca atacgccgtc catggcttcc ttgatttccg ctttgttgac tttgccaatg
     2041 tcctgcgact gtacaaagtt ccgttccttg gctatggcat tggcggcttc cgttgccttt
     2101 ttccctatcc agttgtcccg gtacagttcc ccggaaaggg aaacccggtt tgccagtatg
     2161 tgcaggtggg cttgctcttt cttgctctcc gtaccgctgt gccggatgat gatgtactgg
     2221 tggttcgcca accccatgcg ctgcatgaag tcgttaccaa gttccgccca gtccgcatcc
     2281 gtaaaggtgg cgctttcctc tatcgagggg ctgacctcga aacgtaggca gttgtttttt
     2341 acgttcggaa aatcaatgtg atacggtttc atttcctgca ccatttccgc accagtacac
     2401 ccataaagtt catgccgggc tatctcggtg gctactgcct gcccgtttat ctctttggcg
     2461 aggtcgtact ccaatgccgc cacaccatgt gatatgcttt tccctttccc tatcatcgtg
     2521 agagtatttt tttgagctct gtaattagtg cccggttctc ctcgaacact tcccggtact
     2581 ggcgaccacc gaagtagttg ttcagccgtt ggagcgttcc tttcaactgg gctatgtccc
     2641 ggagaagctg ccgttcctcc ttagactgca ccgggctgcc tgctccgcaa gtgcgtcctt
     2701 ttcaagttcg gtacaccgga atgtaatctt ttcgttccgt cttagttcca tgccacaaag
     2761 gtaatcttaa aatctttttg agcaaagcga gaaaagcaag aattgtccga caaggacact
     2821 tcttgctata tatacacatg ccgcatggga atacatccac gccaaccgcc cagcaaaaag
     2881 ccccggcgtg ttagaaaatc ccccacagcc taacaaattc taccccattt ctacccaagg
     2941 ggattacacg ccgcagaagt catccgggaa catgtcttcc agttcgtcca aatcaatggt
     3001 ttcttcttcg gacacctcca cccattcaat gttttctttc cctcttatcg cacatgagta
     3061 catctcggcg gcggcaagag aagagaacac ctcgtgcata ggctcgccgt tctctaacca
     3121 gtaaacacaa tattcgacca taacagtttt atcatttatc gttgtaatgc ccctctacct
     3181 ccaagatgta gacctctacc acttcctcgt aaatgtcata gattatccgg tcatgggcgg
     3241 taatgtgtcg agaccatgta atatccccac cgccccttaa cgcttcggga tgcccaagcc
     3301 cagtcttagg gtgctctaac aattctttgt agattttctt atacttcttg aacaaattgg
     3361 ggtttgattt cttccatttg gcaatcacct tatccacccc gtcggaaacc ctaattgtgt
     3421 acatcataga ctatcgaaat aagcgtcaat atcctcactg cttttgaggg taacacactt
     3481 tccggatttg atttcttccc ttgccttgtc aatccttgct tgcagctccg gggttatcat
     3541 caaatcttca cgaccaactt ttaccaaagc gtaaatctcg tttttcctgc gaattagtac
     3601 ctgctcgcca ttgtcagctt tggtgaaaga cgctgcgagg ttgttacggt attctcttac
     3661 tgataatgct tccatatctg tttgctttta aattcagcac aaagatagct atatttcaat
     3721 aaaatacaaa cattttgtac acaaacgtgt acacgccata aaaacccgtt tccaatccta
     3781 ccgcccgttg gttggttttg ctttgctctt tttccctatc gtttttcttt ttccgacagt
     3841 aaatcaacca cttagctcta taaattcccc ttgtctgtta taagcatcgt ctgtaatatg
     3901 ctctatcact tcttcgcaaa tgcccataaa taaagggatt agaacatgtg tttccccaca
     3961 ctttgggagt ttagtcccca cactttggga gtttaaaagt aaattagtcc ccacactttg
     4021 ggagttttac ccctcttttt ttgttttaat gtggggattg tgtatatttg cagcaaatca
     4081 atcgttt
//
