LOCUS       CP103160                3003 bp    DNA     circular BCT 29-AUG-2022
DEFINITION  Bacteroides fragilis strain BFG-481 plasmid unnamed2, complete
            sequence.
ACCESSION   CP103160
VERSION     CP103160.1
DBLINK      BioProject: PRJNA646575
            BioSample: SAMN30323385
KEYWORDS    .
SOURCE      Bacteroides fragilis
  ORGANISM  Bacteroides fragilis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Genome Sequencing of Bacteroides fragilis Group Isolates with
            Nanopore Technology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2022) LCIM, National Institute of Allergy and
            Infectious Diseases, 9000 Rockville Pike, Bethesda, MD 20892, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 486x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/22/2022 19:32:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,520
            CDSs (total)                      :: 4,427
            Genes (coding)                    :: 4,130
            CDSs (with protein)               :: 4,130
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 236 of 297
            Pseudo Genes (incomplete)         :: 68 of 297
            Pseudo Genes (internal stop)      :: 15 of 297
            Pseudo Genes (multiple problems)  :: 19 of 297
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Bacteroides fragilis"
                     /mol_type="genomic DNA"
                     /strain="BFG-481"
                     /host="Homo sapiens"
                     /db_xref="taxon:817"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: Bethesda"
                     /collection_date="2003-04-12"
     gene            34..624
                     /locus_tag="NXY37_22555"
     CDS             34..624
                     /locus_tag="NXY37_22555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_process:
                     GO:0006260 - DNA replication [Evidence IEA]; GO_process:
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="UVQ90439.1"
                     /translation="MENKKAVKLTDFQKNEENPFMKQAIEGIENHVVKKYKSNSGGDKR
                     AVVALADTETGEVFKTSFIRQIEVDEEQFTKLYLSNFAAFFDLSQAAIRVFGYFMTCMK
                     PKNDLIIFNRKKCLEYTKYKTDKAVYKGLAELVKAEIIARGPADNLWFINPLIVFNGDR
                     VTFAKTYVRKKTLAAQKKEEAEKRQLSLGFDEQ"
                     /name="replication/maintenance protein RepL"
     gene            1241..1855
                     /locus_tag="NXY37_22560"
     CDS             1241..1855
                     /locus_tag="NXY37_22560"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="UVQ90440.1"
                     /translation="MGATSIHVQAVKPGSEIHNFREKELDYVRPELSHLNESWVGDSIS
                     HRLESAKQRYLDTVGQKMQAKAAPIREGVIVIKQETTMQELQQFATVCKERFGIEAFQI
                     HIHKDEGYMNAKQWTPNLHAHVVFDWTQPNGKSVRLSRDDMAELQTIASETLGMERGVS
                     SDRKHLSAMQYKTECAKEQLQELSNPYTQGRRIHERKAMDT"
                     /name="plasmid recombination protein"
     gene            1830..2597
                     /locus_tag="NXY37_22565"
     CDS             1830..2597
                     /locus_tag="NXY37_22565"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVQ90438.1"
                     /translation="MNAKQWTPNLHAHVVFDWTQPNGKSVRLSRDDMAELQTIASETLG
                     MERGVSSDRKHLSAMQYKTECAKEQLQELSNDISSALDKHKDVQNQLLQLQKELRSIET
                     KKNVQKLISKASEKFYGLIGKTVNDREKDALKAKVKALEGENEQLSDRLGKAILEKEQN
                     GTKAFKAENDKEYYRQQMDNARTTSNRLRTENQELKTETKELKKELGKMKDLFNSEQLE
                     ALRHHFPNISKAMEEGKDLLKQITRSRGFGMGM"
                     /name="hypothetical protein"
     Typing          2..614
                     /name="rep_cluster_1868"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.701"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1798..2594
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.617"
                     /coverage="100.0"
                     /evalue="2.42e-166"
ORIGIN
        1 gtggagaata atatttccca aaaacacatc aaaatggaaa ataaaaaagc agttaagtta
       61 accgattttc aaaagaacga agaaaatcct tttatgaaac aagctataga aggtattgaa
      121 aatcatgttg ttaaaaagta taagagtaat agtggtggcg ataagagagc cgtagtagct
      181 ttagccgaca ctgaaactgg agaagtgttt aagacttcgt ttatccgtca aatagaagta
      241 gatgaagaac aattcactaa attgtatctt tctaactttg ctgcattctt tgacctatca
      301 caagcagcta ttcgggtttt tggttacttt atgacctgca tgaaacccaa aaatgattta
      361 atcatcttca atagaaaaaa atgcctagaa tataccaaat acaaaacaga caaagccgtt
      421 tataaaggac ttgcagaact tgtaaaagct gaaatcatag cccgaggacc agccgataat
      481 ctttggttta ttaatcctct gatagtattc aatggtgacc gagtgacatt tgctaaaaca
      541 tacgttcgga aaaagacttt agctgcccaa aagaaagaag aagcagagaa acgacaatta
      601 tcacttggct ttgatgaaca gtaacactcc attgagtgaa gctgccgttt ggtcgctccc
      661 cctttggggg ggggggggat agataaagtt cctctatgta aagttataat gggggatgaa
      721 aggcaaggtt cgctaacctt accccgattg tttatgcttc ccagcattca atctatcccc
      781 gtttatgggt ttctctttca ccctacaaag ataacctcat gggggaaaaa tgtccaagaa
      841 tatggggtaa aaactatcaa gtcggtagaa aataagtatc tttgaagtac atttttagta
      901 gaggtactgg tatgcctaga cgaagcagat aggcaaaaat aaagccgctg gagatgtcgg
      961 atgtccgtca attaactcca gcggcttgta ttttggtaac tctcccgtat cggtcggcta
     1021 ccgtttggtc agcaacaaag gtagtacttt attgtcgcaa atccaaatgt tttccccttt
     1081 atttgtgcat ttacagaaca tttgctaact tcgcacctat gcagtatggt gatagcacac
     1141 tagcacccta aagggtgcgt gtgtaagagg acaagccccc ttaaccccct gtcagccggc
     1201 tgtcaccggc tgttatcgat tgtcacaaaa atgttaactt atgggagcaa caagtattca
     1261 tgtacaagca gtgaagccgg ggagtgagat tcacaacttt agggaaaaag agttggacta
     1321 tgttcgtccc gaacttagtc atttgaatga aagctgggtt ggagatagca tctcccatcg
     1381 gctggaaagt gcgaaacaaa gatatctcga tacggttggg cagaagatgc aggctaaagc
     1441 cgcacccata cgagagggag taatagtaat caaacaagaa accaccatgc aggaactcca
     1501 gcagtttgcc acggtctgca aagaacgttt cggtatcgaa gcatttcaaa tccatataca
     1561 caaggacgaa ggatacatga acgcaaagca atggacacct aaccttcatg cccatgtagt
     1621 tttcgattgg acgcagccga atgggaaaag tgtgcgttta tcgcgtgatg acatggcaga
     1681 actccaaacc atagcatctg aaacactggg catggaacgt ggcgtttctt ctgaccgcaa
     1741 acacttatcg gctatgcagt acaagaccga atgtgcgaaa gaacagcttc aggaactttc
     1801 aaatccatat acacaaggac gaaggataca tgaacgcaaa gcaatggaca cctaaccttc
     1861 atgcccatgt agttttcgat tggacgcagc cgaatgggaa aagtgtgcgt ttatcgcgtg
     1921 atgacatggc agaactccaa accatagcat ctgaaacact gggcatggaa cgtggcgttt
     1981 cttctgaccg caaacactta tcggctatgc agtacaagac cgaatgtgcg aaagaacagc
     2041 ttcaggaact atcaaacgat atatccagtg cattagacaa acacaaggac gtacaaaacc
     2101 agcttcttca gcttcagaaa gaactacgtt ccattgaaac aaagaagaac gtccaaaaac
     2161 tcatttctaa ggcttcagag aagttttacg gcttgattgg aaaaacagtc aacgataggg
     2221 aaaaggatgc cttaaaagcc aaagtaaagg ctttagaggg ggaaaatgaa cagctatccg
     2281 atagactggg aaaggctata cttgaaaaag agcaaaacgg cacaaaggca ttcaaagccg
     2341 agaatgacaa ggagtattat agacaacaaa tggacaacgc aaggacaacc agtaaccgat
     2401 taaggacgga gaaccaagaa ctaaagaccg aaaccaagga attgaaaaaa gaacttggta
     2461 agatgaaaga cttgttcaat tccgaacaac tggaggcttt aaggcatcat ttcccaaaca
     2521 tatcaaaagc tatggaagag gggaaagacc tactcaagca aatcaccaga agcagaggtt
     2581 tcggtatggg tatgtaaccc cccccccgcc cccaaagggg gagcgaccaa acggcagcct
     2641 ctctcaatgg agtgttacgt cgttcggatt cggaagaaat tttgcagggt tcggaaaacg
     2701 gttgtgtgct tattctaaaa aaagtttatg gtgtggcgct cgtcgggacg ggtggtcccg
     2761 ccccttgccg ctgggcgctc cccgaccgat gatttttaag tgactgattt tgtgctgttt
     2821 tgggggtata ttaagaatag aagaaataga ataagttaag tacttgatac acaatatagg
     2881 gcattttcca tattggaaat tctcattttc caatctagaa aatactgatt tcctaatata
     2941 atactaaaca ggaaaataat atttccctta atattgtttt tatggaaaat aatagtttac
     3001 ttt
//
