LOCUS       CP103133                5609 bp    DNA     circular BCT 29-AUG-2022
DEFINITION  Bacteroides ovatus strain BFG-299 plasmid unnamed3, complete
            sequence.
ACCESSION   CP103133
VERSION     CP103133.1
DBLINK      BioProject: PRJNA646575
            BioSample: SAMN30323268
KEYWORDS    .
SOURCE      Bacteroides ovatus
  ORGANISM  Bacteroides ovatus
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 5609)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Genome Sequencing of Bacteroides fragilis Group Isolates with
            Nanopore Technology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5609)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2022) LCIM, National Institute of Allergy and
            Infectious Diseases, 9000 Rockville Pike, Bethesda, MD 20892, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 175x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/22/2022 20:35:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,694
            CDSs (total)                      :: 5,602
            Genes (coding)                    :: 5,202
            CDSs (with protein)               :: 5,202
            Genes (RNA)                       :: 92
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 400
            CDSs (without protein)            :: 400
            Pseudo Genes (ambiguous residues) :: 0 of 400
            Pseudo Genes (frameshifted)       :: 276 of 400
            Pseudo Genes (incomplete)         :: 84 of 400
            Pseudo Genes (internal stop)      :: 70 of 400
            Pseudo Genes (multiple problems)  :: 28 of 400
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5609
                     /organism="Bacteroides ovatus"
                     /mol_type="genomic DNA"
                     /strain="BFG-299"
                     /host="Homo sapiens"
                     /db_xref="taxon:28116"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA: Bethesda"
                     /collection_date="1991-06-06"
     gene            1..597
                     /locus_tag="NXX44_28355"
     CDS             1..597
                     /locus_tag="NXX44_28355"
                     /inference="COORDINATES: protein motif:HMM:NF013258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="UVQ67436.1"
                     /translation="MAKTCIRVEACNIGSSERHNLRSKELDYIRPELTHRNEQWVECSI
                     AEVHRDITEKYKEATGQGLQKKATPIREGVIVISEETTIQQLQDLAEKLEERFGVHAFQ
                     IYTHKDEGANVWDGKEEAWKPNYHAHMIFDWTDGHTGKTVKLNRHDMAEMQTITAECLN
                     MERGVSSDKKHLSAMQYKNKWKQRKLNNFKKTSSN"
                     /name="plasmid recombination protein"
     gene            549..1250
                     /locus_tag="NXX44_28360"
     CDS             549..1250
                     /locus_tag="NXX44_28360"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein 22"
                     /protein_id="UVQ67437.1"
                     /translation="METEKAEQLQKDIEQLNRAYTAGTEKITTVQKELSTAQKELNSMK
                     TDIHINEAKEAAAKAGKTVFNAVTSVFNVGEVKRQAKEIETLKKENYNLSFKNQNLEGQ
                     LRTNNIEMRRVQETTDRQIKAEASKLKPITNLFPEMENAQENIEELQTMGIQNKDIRQL
                     LIGKEIYYTGNLYDKDKRKSYQVKNVKIDISKSQNGITTIWLNNIHFKNFLKELWNTLQ
                     KTLDNGNWLHR"
                     /name="coiled-coil domain-containing protein 22"
     gene            complement(1320..2090)
                     /locus_tag="NXX44_28365"
     CDS             complement(1320..2090)
                     /locus_tag="NXX44_28365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVQ67438.1"
                     /translation="MKKVMFMFISLLTVLGVCSCNDNEVIEPVVSDSVSDMEVLSRFVD
                     VNEITNEYYFNENKKTRALSYVTGSDWQDLEKVSPLSIEKYKNNLQVLNAQVASAISNP
                     NTAYVVFSVNGKTLVKKVKEDANFDFSVFRDVVTETRAVLPSLSINGGSQSTTGVFYDS
                     SRTLKMQVDLNASIQNNYYFFEVLNPNAKPSPDDNITTPESVAFSGTGPLWSNTFTWTS
                     YWDANVPGQGFKWEFKGKGTTPSFGFIANCTFSR"
                     /name="hypothetical protein"
     gene            2182..2658
                     /locus_tag="NXX44_28370"
     CDS             2182..2658
                     /locus_tag="NXX44_28370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVQ67439.1"
                     /translation="MNRIIHISLLISTVSLFLISNTGCVEKQGYYNHGEESIISLICDI
                     TWAGKKTTDENGSVWQGTYKFNKNGTYTRTNIEIDKQGNKKEANIYGQWSFGDPSFSTI
                     YFGGEHYWDIDELTKNKFSFYDRSGKFGDPFMNREYIELTPYQENNTTENNTTN"
                     /name="hypothetical protein"
     gene            complement(2662..3711)
                     /locus_tag="NXX44_28375"
     CDS             complement(2662..3711)
                     /locus_tag="NXX44_28375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="UVQ67440.1"
                     /translation="MCKDRTNITNCKESPLKNGSDSLKKKAKVKIISHNFTRKLSEKNP
                     DSKLMKAYMDAHVCSNVYTVQGGKAHSHYCHRAFCPICQRIQTAQNVKKFLPVMKYLAS
                     EGREFYFVTLTLQNCVTDDARVLRDFMRRCNRMWADGIRTKHKFRSLGISGVIKKECTY
                     HVVKKDLFSFHYHFHIIVDSLEAAKYVVAQWKKLHGSTVADARFQKYKKIEDFENAAIE
                     VFKYASKASVSKSKGRDGKKKIQINYKALDMIYTAMHGMQRMSSFGQFRTMIGDEALND
                     FRDEDLVLNIEGLNVEDGCYTWYMSVKDKVADWYNMETGEALCCYKVTRKDELLQDIYL
                     DEDIFPNSG"
                     /name="protein rep"
     gene            complement(4207..4605)
                     /locus_tag="NXX44_28380"
     CDS             complement(4207..4605)
                     /locus_tag="NXX44_28380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-poly-alpha-D-galacturonosidase"
                     /protein_id="UVQ67433.1"
                     /translation="MKPTDYIEWDNLKDIPFFLCQVVEDREKQDLDIYYLGKRVLHDYD
                     HVGHYLRTAVILFRRVKSRTADWVNLRNLWTLRNCVRENYNHGIGMNDLIFGENFDGDN
                     LDTLTPLTKKRFDFLCKRIKELDPYATI"
                     /name="exo-poly-alpha-D-galacturonosidase"
     gene            complement(4642..4962)
                     /locus_tag="NXX44_28385"
     CDS             complement(4642..4962)
                     /locus_tag="NXX44_28385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="UVQ67434.1"
                     /translation="MVEQYEVYWVELDPTRGGEMAKTRPCVVVTPSDLNMYLTTVVIVP
                     ITSTIRNYPYRVLCSVAGREGEIATDQIRTVDKSRLKRKIGDLNFSEIKRLREVFQQMF
                     CE"
                     /name="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            complement(4956..5204)
                     /locus_tag="NXX44_28390"
     CDS             complement(4956..5204)
                     /locus_tag="NXX44_28390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="UVQ67435.1"
                     /translation="MVTNIIQIGNSKGIILPSEVLKQLRLSLKSAVSISLDGNNIVIKA
                     QPRQGWAEAAKRAHENGDDELLIPDVFEDEKFEDWTW"
                     /name="AbrB/MazE/SpoVT family DNA-binding domain-containing
                     protein"
     Typing          complement(2663..3711)
                     /name="rep_cluster_870"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.81"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          547..1247
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.573"
                     /coverage="100.0"
                     /evalue="5.62e-141"
ORIGIN
        1 atggcaaaga catgtatcag agtggaagcc tgcaatatag gcagctcgga acgtcacaac
       61 ttaagaagca aggagctcga ctacatccgt ccggaactca cacaccgcaa cgaacagtgg
      121 gtagaatgtt ccattgccga agtacatcgg gacataacgg aaaaatacaa ggaagccacc
      181 ggacaaggat tacagaagaa agctacccca atccgggaag gggtaatagt aatcagcgag
      241 gaaaccacaa tccagcagct tcaagacttg gccgaaaaac tggaagaacg ctttggagtg
      301 cacgccttcc aaatctacac ccataaggat gaaggcgcaa acgtatggga cggaaaggaa
      361 gaagcatgga aaccgaacta tcatgcgcac atgatctttg actggacgga cggccataca
      421 ggaaaaactg taaagctgaa cagacacgac atggcggaga tgcagactat aacagcagag
      481 tgtctcaaca tggaacgtgg agtttcttca gacaagaaac acttgtcggc catgcaatac
      541 aaaaacaaat ggaaacagag aaagctgaac aacttcaaaa agacatcgag caactaaaca
      601 gggcatacac tgccggaacc gagaaaatca ccacagtcca aaaggaactt agtacggcac
      661 aaaaagagct aaacagcatg aaaaccgaca tacacatcaa cgaagccaaa gaagctgccg
      721 caaaagccgg aaaaaccgta tttaatgccg ttacaagcgt ctttaatgta ggggaagtaa
      781 aacgacaagc caaagaaatt gaaaccctca aaaaagaaaa ttataacctc tcattcaaga
      841 atcaaaattt agaaggacaa ttaagaacca acaacatcga gatgcgaagg gtacaggaaa
      901 caaccgacag acagataaag gcagaagcat ccaaactaaa acccataacc aacctttttc
      961 cggaaatgga aaatgcacag gaaaatatag aggaactcca aactatggga atacaaaata
     1021 aagatatccg acaacttctt attggaaagg aaatatacta cactggaaac ctttacgata
     1081 aagacaagag aaaatcatac caagttaaaa acgtgaaaat agatatctcc aaaagtcaga
     1141 atggtattac tacaatttgg ctaaataaca tccactttaa aaactttctt aaagaacttt
     1201 ggaatacact acaaaagact ttagataacg gtaactggct acaccgatag aaagacacta
     1261 aaaagaattg cacttctctt ttttgagaag tgcaattcaa attaaatccc gttcattttt
     1321 taacgactaa aggtgcaatt agctataaaa ccaaaagaag gagtagtacc ttttccttta
     1381 aactcccatt taaaaccttg accaggtaca tttgcatccc aatacgaagt ccaagtgaaa
     1441 gtgttactcc ataacggtcc agttccacta aaagcaacac tttcaggtgt cgttatatta
     1501 tcatccggac ttggtttagc attaggattt aatacttcaa agaaataata attattctgt
     1561 attgaagcat tcaaatcaac ttgcatcttt aaagtacgac tggaatcgta aaaaactccg
     1621 gtagtgcttt gagaacctcc attaatactc aaagaaggga gaacagccct cgtttccgtt
     1681 acaacatccc taaatactga aaaatcaaaa ttagcatcct ccttcacctt tttaaccaaa
     1741 gtctttccat ttacagaaaa aacaacataa gcggtattag gatttgaaat agcacttgcc
     1801 acttgagcat ttaaaacctg caggttattc ttatatttct caatactcaa aggactaact
     1861 ttttctaaat cctgccaatc cgaacctgtt acataagaca acgccctcgt tttcttattc
     1921 tcattgaaat agtattcatt tgtaatctca tttacatcaa caaaacgaga taagacctcc
     1981 atatcagata cagaatcact aaccacaggt tctattactt cattatcatt acacgaacaa
     2041 acacccaaaa cagtcagtag actgatgaac ataaacatta cttttttcat aaatcctcct
     2101 tttttaaagt taataaataa aggtttcgcc caaaaatata aaatttctcc tatttttgca
     2161 ttgaatatta aatatttaac tatgaataga ataatacata tctctttact tatttcaaca
     2221 gtatcacttt ttcttatttc aaacaccgga tgtgttgaaa aacaaggata ctacaaccat
     2281 ggagaagaaa gcataatctc attaatttgt gatattacat gggctggtaa aaaaacaaca
     2341 gacgaaaacg gcagtgtctg gcaaggtaca tacaaattca ataaaaacgg aacatatacc
     2401 cgtacaaata tagaaatcga caaacaagga aataaaaaag aagccaatat ctacggacaa
     2461 tggtcattcg gtgatccaag tttcagtacc atttattttg gtggagaaca ttactgggac
     2521 atagacgagc taactaaaaa caaattttcc ttctatgaca gaagtggaaa atttggcgat
     2581 cctttcatga atagagaata catagaactc acaccatacc aagaaaacaa tacaacagaa
     2641 aacaatacaa caaactaaca actacccact attaggaaaa atatcctcat ctaaataaat
     2701 atcctgcaaa agttcatcct tccgggtgac cttataacaa cacaatgcct ccccagtttc
     2761 catattatac caatcagcca ccttatcttt cacagacata taccaagtat aacaaccatc
     2821 ttcaacattc aatccctcaa tattaagaac caaatcctca tctctaaaat cattcaacgc
     2881 ttcatctccg atcatggtgc ggaactgtcc gaaactactc atgcgctgca tcccatgcat
     2941 ggcagtataa atcatatcca aagccttata atttatctga atcttttttt taccgtcacg
     3001 acctttcgat tttgagacag aagcctttga agcatactta aaaacctcta tcgctgcatt
     3061 ttcaaaatcc tctatcttct tatatttctg aaaacgggca tcagcaaccg tactaccatg
     3121 caatttcttc cattgggcta ctacatattt tgcagcttcc aatgaatcaa caataatatg
     3181 aaaatgataa tggaacgaaa acaaatcctt ttttactaca tgataggtac actccttctt
     3241 aatcaccccc gaaataccaa gtgaacgaaa cttgtgtttc gtccttatac catcagccca
     3301 catccggtta catctacgca tgaaatctcg caataccctt gcatcatcgg ttacacaatt
     3361 ttgcaaggta agcgtcacaa aataaaactc tctgccttcc gaagccaaat acttcataac
     3421 cggaaggaac ttctttacat tttgtgccgt ttgaatacgc tgacagatag gacaaaaagc
     3481 ccgatgacaa taatgggaat gcgccttacc tccctgcaca gtataaacat ttgaacacac
     3541 atgggcatcc atatatgctt tcatcagctt actatccgga ttcttttccg aaagttttcg
     3601 agtaaaatta tgggatatga tttttacttt agcctttttt ttaagactat cagaaccgtt
     3661 ttttaacgga ctttctttgc agttggttat atttgttcta tctttgcaca tatgaaattt
     3721 tgatgtggct ttcgtttggt atccgccccg ccaagaacga acacaaacga ttgctaattc
     3781 ttaaaataca aagcccgttg gttactgcct tcgggctttc gttttttctc agcccaaaag
     3841 taggtaattc attccaaacc gccaaacatc attcaaaaaa cctacatata acaagtcaaa
     3901 aagcatttac cacgaaactt tctagtttat ctagtatggg ggctcgcccc cattcccccg
     3961 gctgcgcaat tctgacacaa atacactact aaatccacaa cagacaaaga tacttccgga
     4021 aaaaaaacta tctttgccgt acacaaagac aacccttgcc gagcgatacg ggagagttgc
     4081 aaaaaaaaca ccggagtgaa ttgacggata tccgacatct ccggtggtat ttttttttct
     4141 tatccccctc ccgtccccca aagggggagc acacaaagac aagccctgcc ttcactcaat
     4201 ggagtgttaa atagttgcat atgggtcaag ttccttaatc cgcttacaca aaaaatcaaa
     4261 gcgtttcttt gtaagtggtg taagcgtatc gagattatct ccatcgaaat tctccccaaa
     4321 aataaggtcg ttcattccta tgccgtgatt gtagttctca cgcacacagt tgcgcagcgt
     4381 ccaaaggtta cgcaggttta cccagtctgc tgtgcggctc tttaccctgc ggaacaagat
     4441 aacggcagtc cgcaaatagt gccccacatg gtcgtaatcg tgcaagacac gtttgcccaa
     4501 atagtaaata tccaaatctt gtttttctct gtcctcgact acttgacaga ggaagaatgg
     4561 aatatccttc aggttatccc attcgatgta gtcagttggt ttcataatgt cttatttgca
     4621 tttttctttc cgtactttcc attattcaca gaacatttgc tgaaatactt cacgtaacct
     4681 ttttatctca ctgaaattca aatctccaat ctttcttttc agccggcttt tatccaccgt
     4741 gcgaatttga tcagtggcta tctctccttc acgcccggct acagaacaca gaacccggta
     4801 aggataattt cttatcgttg aggttatcgg aacaatgact actgttgtaa ggtacatatt
     4861 caaatctgaa ggagtgacaa ctacacatgg ccgtgtcttt gccatttcac ccccacgggt
     4921 agggtctaac tctacccaat acacttcata ctgttctacc atgtccaatc ctcaaacttt
     4981 tcatcctcaa acacatcagg gataagcaac tcatcatccc cattctcatg ggcacgtttg
     5041 gcagcttccg cccatccctg ccgtggttgt gcctttatca caatattgtt cccgtccaaa
     5101 gaaatgctca cagccgattt caaggacaaa cgcaactgtt tcaatacttc ggaaggaagt
     5161 attatcccct tgctgtttcc aatctgaata atattcgtta ccataatgtt ataactttat
     5221 aattgattat cacgatacaa atatatataa agttataaca ataacaaaag gaaaaacaac
     5281 ttttttccat tttcaccgag aaccctgtca atggctgcac tcgtcgggag tgttggtgca
     5341 cccccttgcc gtgcagcgct ccccgaccga tgatttttta ttgtgccttg tgtccttttc
     5401 ccttttgtcc ggcttctgtt cccaagcaca tccggaacac atggcagagc gcttgcaaat
     5461 cccgtccgga aggcttacct ttgcaaaagg ttatagcaca ctataacccc taaaggggtt
     5521 gtgtgcgagg ggtgtcccct cgaccccgtg cgatgtgacg tttcacgcca catcttctaa
     5581 acagacatac acacctaaaa cctaaagat
//
