LOCUS       CP103124                4121 bp    DNA     circular BCT 29-AUG-2022
DEFINITION  Parabacteroides distasonis strain BFG-308 plasmid unnamed2, complete
            sequence.
ACCESSION   CP103124
VERSION     CP103124.1
DBLINK      BioProject: PRJNA646575
            BioSample: SAMN30323273
KEYWORDS    .
SOURCE      Parabacteroides distasonis
  ORGANISM  Parabacteroides distasonis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tannerellaceae;
            Parabacteroides.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Genome Sequencing of Bacteroides fragilis Group Isolates with
            Nanopore Technology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2022) LCIM, National Institute of Allergy and
            Infectious Diseases, 9000 Rockville Pike, Bethesda, MD 20892, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 313x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/22/2022 19:12:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,443
            CDSs (total)                      :: 4,340
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 103
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 193 of 232
            Pseudo Genes (incomplete)         :: 46 of 232
            Pseudo Genes (internal stop)      :: 12 of 232
            Pseudo Genes (multiple problems)  :: 15 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Parabacteroides distasonis"
                     /mol_type="genomic DNA"
                     /strain="BFG-308"
                     /host="Homo sapiens"
                     /db_xref="taxon:823"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: Bethesda"
     gene            join(3371..4121,1..44)
                     /locus_tag="NXX66_22180"
     CDS             join(3371..4121,1..44)
                     /locus_tag="NXX66_22180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="UVO67662.1"
                     /translation="MIGKGKSISHGVAALEYDLAKEINGQAVATEIARHELYGCTGAEM
                     VQEMKPYHIDFPNVKNNCLRFEVSPSIEESATFTDADWAELGNDFMQRMGLANHQYIII
                     RHSGTESKKEQAHLHILANRVSLSGELYRDNWIGKKATEAANAIAKERNFVQSQDIGKV
                     NKAEIKEAMDGVLKKMQGFDFTKFKEELGKRGFKVREARASTGKLNGYYVTARSGTEYK
                     ASEIGKGYTLAHIERTQSKLKCNSMNISHGNKLTPGSGSFQR"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1..453
                     /locus_tag="NXX66_22185"
     CDS             1..453
                     /locus_tag="NXX66_22185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVO67663.1"
                     /translation="METNSHPEAAASNVKQGQYSPRKRKDTTPRVNPQLAVELVYQELK
                     RVEVYTKRIEDATARKVQIDGKSLESAENRLKNVLADFERQGYRMKNGGYVDKRISFYS
                     ILCAVISLLFACFMCYLWTDAAKDRDNYKQYYEYYQEQAREQKGNK"
                     /name="hypothetical protein"
     gene            473..703
                     /locus_tag="NXX66_22190"
     CDS             473..703
                     /locus_tag="NXX66_22190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005683050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="charged multivesicular body protein 2b-B"
                     /protein_id="UVO67664.1"
                     /translation="MGTTEHEEPRFFFILNKGAKSGGEITHAVLNGSIVSKPAGWDAFH
                     GLALAREKLSSEEIQQQMKELGVEMEIVPLI"
                     /name="charged multivesicular body protein 2b-B"
     gene            complement(740..1765)
                     /locus_tag="NXX66_22195"
     CDS             complement(740..1765)
                     /locus_tag="NXX66_22195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008657007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UVO67665.1"
                     /translation="MKKKLPITKNKDVVVSWVYTWSKQQDMSIHEQRIVLRILEACQAE
                     LKGVKLKDYAGTKRKFEHGLWDVDAQMHVSDVIFSGRDYNEIIAALDSLAGRFFTYEDD
                     EEWWKCGFISNPKYKKRTGIITFRVSNDLWDVFTKFAKGYREFELNKALALPTGYSLRF
                     YMLMSGQVYPLDISLENLKDRLGIPADKYKDKNGKDRIDHFEERVLKPAKAALDESCPY
                     TFNYVKVRENPNNKRSKVTGFRFYPVYQPQFRDEELEGKELQAKVTARYQIDSHVYEYL
                     RYSCGFTSEEINRNKETFITAQEKITDLIGELALLNGKSREKNNPKGWIINALKGKIKD
                     K"
                     /name="replication initiation protein"
     gene            2178..2402
                     /locus_tag="NXX66_22200"
     CDS             2178..2402
                     /locus_tag="NXX66_22200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVO67658.1"
                     /translation="MEALSVREYRNNLAASFTKADNGEQVLIRRKNEIYALVKVGREDL
                     MITPELQARIDKARTLKSSEDIDAYFDSL"
                     /name="hypothetical protein"
     gene            2399..2674
                     /locus_tag="NXX66_22205"
     CDS             2399..2674
                     /locus_tag="NXX66_22205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004519 - endonuclease activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YoeB family toxin"
                     /protein_id="UVO67659.1"
                     /translation="MMYTIRVSDGVDKVIAKWKKSNPNLFKKYKKIYKELLEHPKTGLG
                     HPEALRGGGDITWSRHITAHDRIIYDIYEEVVEVYILEVEGHYNDK"
                     /name="type II toxin-antitoxin system YoeB family toxin"
     gene            2685..2882
                     /locus_tag="NXX66_22210"
     CDS             2685..2882
                     /locus_tag="NXX66_22210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVO67660.1"
                     /translation="MVEYCVYWLENGEPMHEVFSSLAAAEMYSCAIRGKENIEWVEVSE
                     EETIDLDELEDMFPDDFCGV"
                     /name="hypothetical protein"
     gene            3075..3374
                     /locus_tag="NXX66_22215"
     CDS             3075..3374
                     /locus_tag="NXX66_22215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVO67661.1"
                     /translation="MELRRNEKITFRCTELEKDALAEQAARCSLSVSEYCRSLSLGGRP
                     RERYTEEERQLLRDIAQLKGTLQRLNNYFGGRQYREVFEENRALITELKKILSR"
                     /name="hypothetical protein"
     Typing          3372..4114
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="81.452"
                     /coverage="94.0"
                     /evalue="4.31e-139"
ORIGIN
        1 atggaaacaa actcacaccc ggaagcggca gcttccaacg ttaagcaggg acagtacagc
       61 ccccggaaaa gaaaagatac aacgccaagg gtaaacccgc agcttgcggt agaactggtg
      121 tatcaagaac taaagcgtgt tgaagtctat acgaagcgca tagaggacgc aacagcccga
      181 aaggtacaaa tagacgggaa aagccttgaa agtgccgaaa atcgcctaaa aaacgtgttg
      241 gcggactttg aacggcaggg gtacagaatg aaaaacggcg gttatgtgga caaacgaatt
      301 tcgttctact caatcctttg tgctgttatc tccctattgt tcgcttgttt catgtgctat
      361 ctttggacgg atgcagccaa agaccgggac aactacaagc agtattatga atactaccaa
      421 gagcaggcaa gggaacaaaa ggggaataaa taaacatcta actttacgaa ttatgggaac
      481 aacggaacac gaagaacccc ggttcttctt catactgaac aagggtgcga agtccggcgg
      541 cgaaatcacc catgccgtcc taaacggtag tatcgtttcg aagccggcag gctgggacgc
      601 ctttcatggg cttgcactgg cgagagagaa actaagcagc gaggaaatac aacagcagat
      661 gaaagaactt ggtgtagaaa tggaaatcgt tcctctgatt tgacaagatt acccggagtg
      721 tttcagccgg gtaatcttgt tacttatcct tgattttccc tttaagggcg tttataatcc
      781 accctttcgg attgttcttt tctcgtgatt ttccgtttag gagagccagt tctccgataa
      841 ggtcggttat cttttcttgt gccgttatga atgtctcttt gttccggttt atctcttccg
      901 atgtgaagcc gcaggaataa cggaggtact cgtacacatg gctgtctatc tgatatcgtg
      961 ctgtaacctt tgcttgcaat tctttccctt ccagttcttc atctctgaac tgtggttgat
     1021 agaccgggta gaacctaaac ccggtcacct tgctacgttt gttgtttggg ttttcacgca
     1081 ctttcacgta gttgaaagtg tatgggcagc tctcatcaag cgcagccttt gctggtttca
     1141 aaactctttc ctcaaaatga tctattctgt cttttccgtt cttgtccttg tatttgtccg
     1201 caggtatgcc aagacggtct ttcaagtttt caagggatat atccaaaggg tacacctgcc
     1261 cactcattaa catgtaaaac ctaagggaat acccagtagg tagcgcaaga gccttgttta
     1321 actcaaattc tctgtacccc ttggcgaact tggtaaacac atcccaaagg tcgttagaca
     1381 cacgaaaggt tataatgcca gtgcgttttt tatattttgg gttagatata aatccgcact
     1441 tccaccattc ctcgtcatct tcgtaagtaa aaaaacgtcc tgccaaagaa tcgagtgcgg
     1501 ctatgatttc attgtaatca cgtccggaaa aaataacgtc agatacatgc atttgtgcat
     1561 caacatccca aagtccatgc tcaaacttgc gtttcgtgcc tgcataatct ttcagtttta
     1621 ctccctttag ttcagcttgg cacgcttcca gtatgcgaag aactattctt tgttcgtgta
     1681 tggacatgtc ttgctgtttt gaccatgtat atacccatga aacaacaaca tctttattct
     1741 tggtaatagg tagtttcttt ttcataaacg attgatttgc tgcaaatata cacaatcccc
     1801 acattaaaac aaaaaaagag gggtaaaact cccaaagtgt ggggactaat ttacttttaa
     1861 actcccaaag tgtggggact aaactcccaa agtgtgggga aacacatgtt ctaatccctt
     1921 tatttatggg cgtttgcgaa gaagtgatag agcatattac agacgatgct tataacagac
     1981 aaggggaatt tatagagcta agtggttgat ttactgtcgg aaaaagaaaa acgataggga
     2041 aaaagagcaa agcaaaacca accaacgggc ggtaggattg gaaacgggtt tttatggcgt
     2101 gtacacgttt gtgtacaaaa tgtttgtatt ttattgaaat atagctatct ttgtgctgaa
     2161 tttaaaagca aacagatatg gaagcattat cagtaagaga ataccgtaac aacctcgcag
     2221 cgtctttcac caaagctgac aatggcgagc aggtactaat tcgcaggaaa aacgagattt
     2281 acgctttggt aaaagttggt cgtgaagatt tgatgataac cccggagctg caagcaagga
     2341 ttgacaaggc aaggaccctc aaaagcagtg aggatattga cgcttatttc gatagtctat
     2401 gatgtacaca attagggttt ccgacggggt ggataaggtg attgccaaat ggaagaaatc
     2461 aaaccccaat ttgttcaaga agtataagaa aatctacaaa gaattgttag agcaccctaa
     2521 gactgggctt gggcatcccg aagcgttaag gggcggtggg gatattacat ggtctcgaca
     2581 cattaccgcc catgaccgga taatctatga catttacgag gaagtggtag aggtctacat
     2641 cttggaggta gaggggcatt acaacgataa atgataaaac tgttatggtc gaatattgtg
     2701 tttactggtt agagaacggc gagcctatgc acgaggtgtt ctcttctctt gccgccgccg
     2761 agatgtactc atgtgcgata agagggaaag aaaacattga atgggtggag gtgtccgaag
     2821 aagaaaccat tgatttggac gaactggaag acatgttccc ggatgacttc tgcggcgtgt
     2881 aatccccttg ggtagaaatg gggtagaatt tgttaggctg tgggggattt tctaacacgc
     2941 cggggctttt tgctgggcgg ttggcgtgga tgtattccca tgcggcatgt gtatatatag
     3001 caagaagtgt ccttgtcgga caattcttgc ttttctcgct ttgctcaaaa agattttaag
     3061 attacctttg tggcatggaa ctaagacgga acgaaaagat tacattccgg tgtaccgaac
     3121 ttgaaaagga cgcacttgcg gagcaggcag cccggtgcag tctaagcgta tcggaatact
     3181 gccggagttt gtcccttggg gggcgcccaa gggagaggta caccgaggag gaacggcagc
     3241 ttctccggga catagcccag ttgaaaggaa cgctccaacg gctgaacaac tacttcggtg
     3301 gtcgccagta ccgggaagtg ttcgaggaga accgggcact aattacagag ctcaaaaaaa
     3361 tactctcacg atgataggga aagggaaaag catatcacat ggtgtggcgg cattggagta
     3421 cgacctcgcc aaagagataa acgggcaggc agtagccacc gagatagccc ggcatgaact
     3481 ttatgggtgt actggtgcgg aaatggtgca ggaaatgaaa ccgtatcaca ttgattttcc
     3541 gaacgtaaaa aacaactgcc tacgtttcga ggtcagcccc tcgatagagg aaagcgccac
     3601 ctttacggat gcggactggg cggaacttgg taacgacttc atgcagcgca tggggttggc
     3661 gaaccaccag tacatcatca tccggcacag cggtacggag agcaagaaag agcaagccca
     3721 cctgcacata ctggcaaacc gggtttccct ttccggggaa ctgtaccggg acaactggat
     3781 agggaaaaag gcaacggaag ccgccaatgc catagccaag gaacggaact ttgtacagtc
     3841 gcaggacatt ggcaaagtca acaaagcgga aatcaaggaa gccatggacg gcgtattgaa
     3901 gaagatgcag ggctttgact ttaccaaatt caaggaagaa cttggtaaga gaggttttaa
     3961 agtccgggaa gccagggcaa gcaccgggaa acttaacggc tactatgtca cagcccgaag
     4021 cggtacggag tataaggcaa gcgagatagg gaaaggctac actctcgccc atatcgagcg
     4081 gacacaaagc aaactgaagt gcaactcaat gaacatatct c
//
