LOCUS       CP103123                8935 bp    DNA     circular BCT 29-AUG-2022
DEFINITION  Parabacteroides distasonis strain BFG-308 plasmid unnamed1, complete
            sequence.
ACCESSION   CP103123
VERSION     CP103123.1
DBLINK      BioProject: PRJNA646575
            BioSample: SAMN30323273
KEYWORDS    .
SOURCE      Parabacteroides distasonis
  ORGANISM  Parabacteroides distasonis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Tannerellaceae;
            Parabacteroides.
REFERENCE   1  (bases 1 to 8935)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Genome Sequencing of Bacteroides fragilis Group Isolates with
            Nanopore Technology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8935)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2022) LCIM, National Institute of Allergy and
            Infectious Diseases, 9000 Rockville Pike, Bethesda, MD 20892, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 313x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/22/2022 19:12:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,443
            CDSs (total)                      :: 4,340
            Genes (coding)                    :: 4,108
            CDSs (with protein)               :: 4,108
            Genes (RNA)                       :: 103
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 232
            CDSs (without protein)            :: 232
            Pseudo Genes (ambiguous residues) :: 0 of 232
            Pseudo Genes (frameshifted)       :: 193 of 232
            Pseudo Genes (incomplete)         :: 46 of 232
            Pseudo Genes (internal stop)      :: 12 of 232
            Pseudo Genes (multiple problems)  :: 15 of 232
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8935
                     /organism="Parabacteroides distasonis"
                     /mol_type="genomic DNA"
                     /strain="BFG-308"
                     /host="Homo sapiens"
                     /db_xref="taxon:823"
                     /plasmid="unnamed1"
                     /geo_loc_name="USA: Bethesda"
     gene            1..1656
                     /locus_tag="NXX66_22130"
     CDS             1..1656
                     /locus_tag="NXX66_22130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008669305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVO67652.1"
                     /translation="MQKTYLYLMVCVIGAMSLASCSEDSISPDAGGDGDGDSTTEVPLA
                     QVAKPKPNPWLAQEEYSITHFNSAQTDAFSSAVKDGTFNIDLSKCQMTWSGPVNLMTLA
                     STSPNYMWGMSSDRVSYLDISDGKLERVAEAGLPGITMKTQEQLTRIIADHATYDELSK
                     TVTDILGPVPQMSMANGNYVLCDKDNYAYTNAGQVMARYRLANPNNPKDGIMLDHQIRL
                     TNFTFGSFTLVGATMTYDGHLVVAAQNGLLVLNRALTTIEDSYPLPSDQILTNSICIDE
                     NGGVYVASNSRTPGGKGLMQKLICKDGKISTSQADGAWQAYYDGGPQAPCIKLGHGTGS
                     TPTLMGFGQDKDKLVVITDGSKRMKLVAFWRDEIPSDAQQVAGYDKRIAGVHEVTCGLG
                     TSTEWIQSEQSVVVGGYDAFVVNNINVTNQEINDKIIGVIAIGPIVKGPQGAECVRWNT
                     KEKKWESKWTRSDVSSVSMIPAVSIKSEMVFVCGWNDASGWEVTGLDWKSGATRHRSIL
                     GKNNRANGAYAIIQYLANGDLLFNSVAGPIRVKY"
                     /name="hypothetical protein"
     gene            complement(2211..3089)
                     /locus_tag="NXX66_22135"
     CDS             complement(2211..3089)
                     /locus_tag="NXX66_22135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008782579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5712 family protein"
                     /protein_id="UVO67653.1"
                     /translation="MNVKIQGSGNGTYANTGSCVAVTNYLQHEDLERMKKGEEVQPFFN
                     QFRDYVSAREVTFKIDNNKAKLSRTDAKFYVITVSPSEKELRCMGRTPQECAEALRRYI
                     RQDVMRNYAEGFGKGLKSDDVEYYAKIHFNRDGESDSDMHAHIIVSRKDRSNTRKLSPK
                     TNHTGKKNCGNVKGGFDRTDFFRKCESSFDRRMGFDREPEESFDYLNAVKNGSPAEIAR
                     QVERAERIRKEKWDKLKAELQSRQEPEKSKNQQVEKTPDIEQPIPKKKQQEEDLELLKK
                     PKRSRGFGMGM"
                     /name="DUF5712 family protein"
     gene            complement(3086..3691)
                     /locus_tag="NXX66_22140"
     CDS             complement(3086..3691)
                     /locus_tag="NXX66_22140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008669301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clindamycin resistance transfer factor btgA"
                     /protein_id="UVO67654.1"
                     /translation="MDKETTTSVTIDRKTFARLDRLAKSNNVSKKEFLSCALEYFEKYG
                     INPVEHESPAKEMQKLIKRCDQVIAFIRKQEQDFLRPACEAMGSTSMRVTMSMDSILTE
                     KKFSQYQKDNDLFMRDLASLAGIREQALDRAEKAVGQSRDMLLKNQQAIYARLDAVTQR
                     QEKIFSYIASYIDAKGKAGLFDDIKALYKNEKNKREKI"
                     /name="clindamycin resistance transfer factor btgA"
     gene            4083..4430
                     /locus_tag="NXX66_22145"
     CDS             4083..4430
                     /locus_tag="NXX66_22145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="UVO67655.1"
                     /translation="MEAKSRFKVKLSNEAQEFLDGLPNKVREKIISNIRKSTFVLDPDL
                     FKKLDDTDIWEFRTFYNKTKYRLLAFWDKDDKINTLVIATHGFIKKTQKTPPKEIAKAE
                     EIRKDYFNSKR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            4436..4750
                     /locus_tag="NXX66_22150"
     CDS             4436..4750
                     /locus_tag="NXX66_22150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="UVO67656.1"
                     /translation="MEAKKFYTLEEIEDKYIGEKGTPKRDAYEEELNSFLIGEAIKQAR
                     QSKNLTQEELGNLIGVQRAQISRIENGKNLTFSTIARVFKAMGISAKLEIGSMGKVALW
                     "
                     /name="helix-turn-helix domain-containing protein"
     gene            5228..5995
                     /locus_tag="NXX66_22155"
     CDS             5228..5995
                     /locus_tag="NXX66_22155"
                     /inference="COORDINATES: protein motif:HMM:NF013235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UVO67648.1"
                     /translation="MEKKDKKQRDIVLSQDNALTTARYKFDLIEKRALYSIIRNIRAQY
                     IENENGRRDLFDDLIITIHEEELKKCGDNTKMQNIYDALKRLRNRDIEINNEKMWLSIG
                     FVNYVKHIKNKSYFEVQVSKEVLPYYVELAQNFTSYSMTVAIALKSTFSQRFYELCCQY
                     RNAGFFFYTVEKLREMFMLEKKYKRGCDFKRRVLDDPQRSCTMPDNAIYTSSSSQRATK
                     ARKLPNTNSTFTQGKPNNKSYWNTKAQNRRSST"
                     /name="replication initiation protein"
     gene            6209..6388
                     /locus_tag="NXX66_22160"
     CDS             6209..6388
                     /locus_tag="NXX66_22160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVO67649.1"
                     /translation="MKTGDKVIVPAEINGYGRDLRAIVTEIEKFAGAIFVTVIFTEPCP
                     EACGRGGVFTMTSS"
                     /name="hypothetical protein"
     gene            6502..7023
                     /locus_tag="NXX66_22165"
     CDS             6502..7023
                     /locus_tag="NXX66_22165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016271957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVO67650.1"
                     /translation="MEKGQESKQKRKTPLTTIAISQEYAKKLDEYLNGTGITRKEFVEL
                     SVDYFQRTGFDLRGEAFDLSPLEKVAGRLEQSAKIMERHNEGTEAVHLLLQAVREQTAK
                     QLPAPELIAHAAEEKAKAEAKSEEQEREIARLRSENSALLEYKEKAYRELCRIRDEQKT
                     IGKIKVNIEL"
                     /name="hypothetical protein"
     gene            7053..7595
                     /locus_tag="NXX66_22170"
     CDS             7053..7595
                     /locus_tag="NXX66_22170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005852953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="UVO67657.1"
                     /translation="MKKFYLFILLPDYAAHEAVYFSQAITADDFAMKKNPKYVNKVVAP
                     TLEPISSIGGFRTLPDYSFDTMPDDYVALVLIGGFGWNTPVAEKVMPIIKSAIEQEKVI
                     GAICNAASFMAKCGIDRETTSKTLQKSSVIQKISVTLSSVIIAIIVCKLLIFNTLRLQQ
                     FFANHHFSGTYISSNSR"
                     /name="DJ-1/PfpI family protein"
     gene            7618..8715
                     /locus_tag="NXX66_22175"
     CDS             7618..8715
                     /locus_tag="NXX66_22175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008781140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="UVO67651.1"
                     /translation="MDTCLDLIALQYTCMIVMAMLAFFVFVSRYYVTCRNRQFEQSRWM
                     IVVALLLFVVHYMCQMRLGWRQQGNDVGVLFNLLFYSPSAILLSWSQLNILRAGHRRWS
                     FMRYGVVGYALMVLCIVAGVISNGSLHIGPMLYVADAIHFFTLLYYTWAPLRELGNVQR
                     RLDSEHGNPADAYLRTMRFGFFVVCAFAVISPLYILSRPLLFVFGPLGLISLSLFIVSF
                     TALGFSMSEGVTEIVTETNDEIAAAKVFREIGPERIAEIDMAVSKWRSEGGFRDSNLTL
                     SSFARRIMVNRTDVASYLTSIYGKNFRTWLSGIRVEEAKALMAAHPEYSNEVVSMECGF
                     SSRVYFQRLFKERTGLTPAEWRRQR"
                     /name="helix-turn-helix domain-containing protein"
     Typing          5229..6195
                     /name="rep_cluster_1097"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.875"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2215..3089)
                     /name="MOBB"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgcaaaaaa cctatcttta tttaatggtg tgtgtcatag gggctatgtc tttagcttcg
       61 tgttccgaag attctatttc tcctgatgct ggtggcgatg gcgatggcga cagcactacc
      121 gaagtgcctt tggcacaagt tgcaaaaccc aaacctaacc cttggcttgc acaggaagag
      181 tatagtatta cgcacttcaa ttcggctcag actgatgctt tctcgtctgc tgtcaaggat
      241 ggaactttca atatcgatct ttccaagtgt cagatgacat ggagcggtcc tgttaatttg
      301 atgactcttg cttcgacttc gcccaattat atgtggggta tgagtagcga ccgcgtgtcg
      361 tatcttgaca taagcgacgg caaacttgaa agggttgccg aggctggctt gccagggatt
      421 acaatgaaga cccaggagca actgacacgt ataattgccg atcatgctac ttacgacgaa
      481 ctttccaaga cggttaccga tatattgggt ccggtgccac aaatgtctat ggccaacggt
      541 aattatgtct tgtgcgacaa ggataactat gcttacacca atgcaggtca ggtaatggca
      601 cgctatcgcc ttgccaatcc taataatccc aaggatggta tcatgttgga tcatcagata
      661 agacttacga attttacctt cggctctttt acactcgtag gcgctactat gacctatgac
      721 ggacaccttg ttgtggctgc tcagaacgga cttcttgtgc tgaatcgtgc tcttactaca
      781 atcgaagatt cataccctct tccatcagac cagattctca ccaactctat ctgcatcgac
      841 gagaatggcg gtgtttacgt agcaagtaac agccgcacac caggcggaaa aggccttatg
      901 cagaaactca tctgcaagga cggcaaaatc tccacctccc aggctgatgg tgcatggcaa
      961 gcctattacg acggtggtcc gcaagcacca tgtatcaaac tcggacatgg cactggttca
     1021 acgcctacac ttatgggctt tggccaagac aaggacaagc tggtagttat taccgatggc
     1081 tctaagcgta tgaaactggt agctttctgg cgtgacgaaa tcccctcaga cgcacagcaa
     1141 gtggcaggat acgacaagcg tatagccgga gtgcacgagg ttacatgtgg tcttggaaca
     1201 tcaacagagt ggatacagtc tgagcagtct gttgtagtag gcggctatga cgcctttgtt
     1261 gtaaacaata ttaatgttac caatcaggaa ataaacgaca agattattgg tgtaatcgct
     1321 attggtccta ttgtcaaagg ccctcaaggt gccgagtgtg tacgttggaa tacaaaggag
     1381 aaaaagtggg agagtaagtg gacgcgcagc gatgtaagtt ctgtaagtat gattcctgcc
     1441 gtaagtatca agtcagagat ggtatttgta tgtggctgga acgatgcttc gggctgggaa
     1501 gttacaggac ttgactggaa gagtggtgcc acacgtcacc gcagcatatt gggcaagaac
     1561 aaccgagcca atggtgccta cgccatcatt cagtatcttg ctaacggcga ccttttattc
     1621 aactctgttg ccggaccaat ccgcgtaaaa tattgagata atttaatcat catataacag
     1681 ttatgtcaat acttcactaa aaaattaccg aagtattgta atgacacacc cgaacatatt
     1741 gaaatgttct atcagttcca taaacaaggc ttctgttctt tattcctaga tacaggtgat
     1801 aaataacaga taagaaacag attataacaa gcctccggcg gggttttcgc tgggggcttt
     1861 tgacctttgc acatataaaa agcaaaaaga aagcgatatc ggagcgtata ccataaaaca
     1921 gaccaccgac tattacaaaa cgatattacg tgcctaactg atcggggcag ttcctccacc
     1981 atgcggacaa atcccggtaa caaaatgaca aaacggacac tcaccaaaga acaacatttt
     2041 tttctttttt cctaaacacc ttccagcgaa gcacagtgta tttgtcaagg gcgaagggta
     2101 aaaaataccg cacgagcgaa gcgagcacag gagatttttt acccggagag tgaaacgtcc
     2161 ctttacaaat gcggcgtgcg gagttacctt tgtgccggaa aatagaaaaa tcacatgccc
     2221 attccgaaac ctctgctcct ttttggcttc ttcaggagtt ccagatcttc ctcctgctgc
     2281 ttctttttgg gaataggctg ctcgatgtcc ggcgttttct ccacctgttg atttttcgac
     2341 ttctccggct cctgccggga ttgaagctct gctttgagtt tgtcccattt ctcctttctg
     2401 atgcgttccg cacgttccac ctgccgggct atttccgccg gacttccgtt cttgacggca
     2461 ttaagatagt cgaagctttc ctccggctcc cggtcaaacc ccatgcgccg atcaaaagag
     2521 ctttcacact tccggaaaaa atccgtccgg tcaaaacctc ccttcacgtt gccgcagttc
     2581 ttcttcccgg tgtggttcgt tttagggctt aacttcctgg tgttgctccg gtctttccgg
     2641 ctgacgatga tatgtgcgtg catatcgctg tcactttcac cgtcccggtt gaaatggatc
     2701 tttgcataat attccacatc atcgcttttc aagccttttc cgaagccctc cgcatagttt
     2761 ctcatcacat cctgccgtat gtaccgccgt aaggcttccg cacactcctg cggagttctg
     2821 cccatgcagc gcagctcctt ctctgaaggg ctgacagtga taacatagaa ctttgcgtca
     2881 gtccggctta acttagcctt gttgttgtct atcttgaatg tgacctccct tgcgctcacg
     2941 tagtcccgga actggttgaa gaacggctgc acctcctccc ctttcttcat cctctccaaa
     3001 tcctcatgct ggagatagtt cgtcaccgca acacaactgc ctgtattggc atatgtgccg
     3061 ttaccgcttc cctgtatctt cacgttcata tcttttccct tttgttcttc tcgttcttgt
     3121 acaaggcttt tatatcatcg aaaagcccgg cttttccttt cgcatcgata tagcttgcga
     3181 tataggagaa gatcttctcc tgcctttgcg tcacagcgtc cagccttgca taaatggctt
     3241 gttggttctt cagaagcata tccctcgact gaccgactgc cttttccgcc cggtccaaag
     3301 cctgctccct tatcccggca aggcttgcaa ggtcacgcat gaataggtca ttgtcttttt
     3361 ggtattggct gaactttttt tccgtaagta tcgagtccat gctcatggtc actctcatgc
     3421 ttgtactgcc catggcttca caagccggac gcaagaaatc ctgctcctgc tttctgatga
     3481 acgctatcac ctgatcacag cgtttgataa gtttctgcat ttcctttgcc ggactctcat
     3541 gctcaaccgg gttgatgccg tacttctcga agtactccag cgcacaggaa aggaactctt
     3601 tctttgatac attattggac tttgccagcc tatcaagccg ggcaaacgtt tttcggtcaa
     3661 tggtgacaga agtggtggtt tccttatcca tacaattgcg gttttaattg ctgtttatat
     3721 tattaaatct ctgatttcca aaggtatgat tttaaccaca atttcaaact aattgcggtt
     3781 aaaaaactta aacggctgat attcagccgt aaccccgtag ggcaagagga aaaacagggt
     3841 aaagcccggt ttcctcttgc tattctttcg actgccgagc aatcgacgaa caccctcccc
     3901 tgcggtacgg cgaaagctcg tacaaggggg acggggagaa gtcggagcga ggcacaaaga
     3961 aagaacctat tccacaaaca aaaaaacaca tcaaaacggc acaacaggct gcataaaata
     4021 tcaatatttt gtttcgctat atggaaacaa aatattatct ttgcaacact aaaaaacttt
     4081 atatggaagc aaaatcaaga ttcaaggtca aattatccaa cgaagcccaa gagtttttag
     4141 acggattgcc caacaaggta cgtgagaaga tcattagcaa tattcgtaaa tccacctttg
     4201 tacttgatcc agatttattc aagaaattgg atgatacgga tatttgggaa ttcagaacgt
     4261 tctataacaa aaccaagtac aggcttcttg ctttttggga taaggacgat aagataaaca
     4321 ctctggtaat cgcaacacat gggtttatca agaaaaccca aaagacacct cccaaagaaa
     4381 tagccaaagc cgaagagatt agaaaagatt attttaattc aaaaagataa agcgtatgga
     4441 agcaaagaaa ttttatacac tcgaagaaat cgaggataaa tacatcggag aaaaaggtac
     4501 tcccaaacgt gatgcctacg aagaagaact taactctttt ttgataggtg aagccattaa
     4561 acaagctcgc caatcaaaga acttaaccca agaggaatta ggtaatctga taggagtgca
     4621 acgggctcaa atctcccgaa ttgaaaacgg taagaattta accttttcca ccattgcaag
     4681 agtttttaag gctatgggga taagtgctaa acttgaaatc ggaagcatgg gtaaagttgc
     4741 gttatggtaa ctaactatac aacaaaaagt tacattaaat ccgggatgcg ttttcgctcc
     4801 cggaaacttc ttgttttata cattaaaagt catatttata caaaatttaa cacgccaaaa
     4861 aatcttccta atccgttgca cttggcttta cttgtttcca ctctctccca ttctccggaa
     4921 attcgtcgta tcaaaaaaca cgctgaaaag tggagcgaag cgacctctat aaaccaaaaa
     4981 agaaacactc cattgcttta cggtaatttt tagtagttct ttttagttac ttttacggca
     5041 tagcataaca cattgtgctg taatatctta caaaggtcat ctacgacaaa tttccgagca
     5101 tctacgacaa atttccgagc atctacgaca aatttccgaa aaaaacggtt atctacgaca
     5161 aatattctac gccatatatt tgtttttgtc gtagataaat atttcctttg cgaaaagcat
     5221 taaagtcatg gagaagaaag acaagaaaca gagggatata gtcctgtcgc aagacaatgc
     5281 gctgactacc gcacgctaca agttcgacct tatcgaaaaa cgtgccctat actccattat
     5341 acgaaatatc cgggcgcaat acatcgagaa cgagaacggc agacgggact tattcgatga
     5401 tctgattatc accatacacg aggaagaact aaagaaatgc ggtgacaata ccaagatgca
     5461 aaacatctac gatgcgctaa aacggcttag aaatcgcgat atagagatta acaacgaaaa
     5521 aatgtggcta agtatcggtt ttgtcaatta cgtcaagcat atcaaaaata aatcgtattt
     5581 tgaggtgcaa gtttccaaag aggtactacc ctattacgtg gaactcgccc aaaacttcac
     5641 gagttactcc atgacggtag caatcgcact caaaagcaca ttctcgcaaa ggttttacga
     5701 actgtgctgc caataccgaa acgccgggtt ctttttctac acagtcgaaa agcttcggga
     5761 aatgttcatg ttggaaaaga aatacaaacg gggatgcgat ttcaaaaggc gtgtacttga
     5821 cgatccgcaa aggagttgta caatgccgga caatgcgatt tatacttcga gttcgagcca
     5881 aagagctaca aaggcaagga agttaccgaa tacaaattct acgtttacac aaggcaaacc
     5941 gaacaacaaa agctattgga atacgaaagc gcaaaacagg cgatcatcta cataacaacc
     6001 actatttcga ggttctctaa aagtgacaaa ggatatgtta agcgtgtagt gaatgccgta
     6061 cagttacacc cggaaatagc cgtacaggtg gcgcaaaaga tacaaaagaa gtttgagcaa
     6121 tatatagaca agccagccaa acagataggg gctatcatcc gggtttgctt aatggaagat
     6181 tttggtatag aataaaaaac atacaagtat gaaaacaggt gataaagtta tcgtaccagc
     6241 cgaaataaac ggctatggca gagatttacg ggcaatagtt acagagatag agaagttcgc
     6301 cggggcaata ttcgtaacag tgatattcac cgaaccatgt ccggaagcct gcggaagagg
     6361 gggggtgttt accatgactt ccagctaata gcaaggtgat ttattttgct ataatcgcaa
     6421 taaattgcaa taatatctgt gataaattgc tataataata cttataacta cctttgcaac
     6481 agaatatagc attttacagc aatggagaaa ggacaggaaa gcaagcaaaa gaggaaaaca
     6541 ccgcttacca ccatagcgat aagccaagag tacgccaaaa aattagacga gtatttaaac
     6601 ggtacgggta taactcgcaa agaatttgtc gagttgtccg ttgattattt ccaaagaacc
     6661 gggttcgacc tccggggtga agcttttgac ctctccccat tagaaaaggt tgccgggcgg
     6721 ctggaacaat ccgcaaagat catggaacga cacaacgagg ggacggaagc ggtgcacctg
     6781 cttctccaag ctgtccggga acagaccgcc aagcaactcc cagcgccgga actgatcgcc
     6841 catgctgccg aagaaaaagc aaaggcagaa gccaaaagcg aagaacaaga aagggagatt
     6901 gcccggctac gctccgaaaa ctccgctttg ctggagtaca aagaaaaggc ataccgggaa
     6961 ctatgccgga tacgtgatga acaaaaaacc atagggaaga taaaagtaaa tatagagtta
     7021 taaaagaaca tttataaata aaaaaatcat gaataaagaa attttattta tttatccttt
     7081 tgccggatta tgcagcacat gaagctgtgt acttttcaca ggctattaca gctgatgatt
     7141 ttgccatgaa aaaaaatcca aaatatgtca ataaggttgt cgcacccacc ttagaaccaa
     7201 taagttcgat tggtggattc cgcacattac ccgattattc gtttgacaca atgcctgatg
     7261 attatgtagc attggtatta attggcggtt ttggttggaa tacacctgtt gcagaaaagg
     7321 ttatgccaat tattaaaagt gctattgaac aagaaaaggt tataggtgcg atttgtaatg
     7381 cagcttcatt catggctaag tgtggtattg accgagaaac aacatcaaaa actttgcaga
     7441 agtcatccgt aatacagaaa atctcagtaa ctttgtcctc ggtaatcata gcaattattg
     7501 tctgtaaact tttgatattt aacactctaa gattacaaca atttttcgct aatcaccatt
     7561 tttcaggcac ttatatttcg tcgaactcac gttaaattat ttaatataca tacaatcttg
     7621 gatacttgct tagaccttat tgctcttcaa tatacttgca tgattgtgat ggccatgctt
     7681 gcattttttg tgtttgtgtc gcgctactat gtaacttgcc gtaacagaca gtttgagcag
     7741 tcgcgttgga tgattgttgt ggcattacta ttgtttgttg tacactacat gtgtcagatg
     7801 cggttgggtt ggcgacaaca ggggaacgat gtgggtgttc tgtttaatct gctgttttat
     7861 tcgccttcgg ctattttgtt gtcatggtcg cagcttaata ttctgcgcgc tgggcataga
     7921 cgttggagtt ttatgcgcta tggcgttgtt ggatatgctc ttatggtatt gtgcatagtc
     7981 gcaggtgtta tttcaaatgg cagtctgcat attggtccta tgctatatgt tgctgatgct
     8041 attcatttct ttactctatt gtattataca tgggcaccgc tgagggaact tggtaatgtt
     8101 caacgtcggc tcgacagtga acatggtaat cctgcagatg cctacctgcg cactatgcgc
     8161 tttggttttt ttgtggtgtg tgcttttgct gtaatttcgc ctctttatat attgtcgcgg
     8221 ccgctgctct ttgtctttgg acctttggga ttgatttcgc tttcgctgtt tattgttagt
     8281 tttacggcgc ttggatttag tatgtccgag ggagtaactg agattgttac cgagactaac
     8341 gacgagatag ccgctgctaa agtctttagg gagattgggc ctgagcgtat tgccgaaata
     8401 gatatggcgg taagcaaatg gcgaagcgag ggcggtttta gagatagcaa cttgacatta
     8461 tcctcgtttg ctcgtcgcat aatggttaat cgtaccgacg ttgcttcgta tctgacttcc
     8521 atatatggta agaatttccg tacatggctt tcgggtattc gcgtggagga ggcaaaggca
     8581 ctcatggctg cacaccccga atatagtaac gaggttgttt ctatggagtg cggtttctcg
     8641 tcgcgtgtgt attttcagcg tctgttcaag gaaaggacgg gcttgactcc cgccgagtgg
     8701 cgccgtcaac ggtaatatac tggttatgtg tgcgtttgct actgaatgta aggtgtaact
     8761 tttgtacgaa ggttacataa taaggcaaag tgtaaccttt cttactgtga cgtatccttt
     8821 ctataagagt gacacacaag tgatgtcttt gaggtttttg atggttgatg cttcctgtat
     8881 ctttgcatga ggaaattcaa actattcaaa tataaattct caaaaacaat tcact
//
