LOCUS       CP103116                8881 bp    DNA     circular BCT 29-AUG-2022
DEFINITION  Bacteroides ovatus strain BFG-177 plasmid unnamed2, complete
            sequence.
ACCESSION   CP103116
VERSION     CP103116.1
DBLINK      BioProject: PRJNA646575
            BioSample: SAMN30323210
KEYWORDS    .
SOURCE      Bacteroides ovatus
  ORGANISM  Bacteroides ovatus
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 8881)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Genome Sequencing of Bacteroides fragilis Group Isolates with
            Nanopore Technology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8881)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2022) LCIM, National Institute of Allergy and
            Infectious Diseases, 9000 Rockville Pike, Bethesda, MD 20892, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 277x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/22/2022 20:44:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,610
            CDSs (total)                      :: 5,516
            Genes (coding)                    :: 4,069
            CDSs (with protein)               :: 4,069
            Genes (RNA)                       :: 94
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 1,447
            CDSs (without protein)            :: 1,447
            Pseudo Genes (ambiguous residues) :: 0 of 1,447
            Pseudo Genes (frameshifted)       :: 1,346 of 1,447
            Pseudo Genes (incomplete)         :: 49 of 1,447
            Pseudo Genes (internal stop)      :: 121 of 1,447
            Pseudo Genes (multiple problems)  :: 67 of 1,447
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8881
                     /organism="Bacteroides ovatus"
                     /mol_type="genomic DNA"
                     /strain="BFG-177"
                     /host="Homo sapiens"
                     /db_xref="taxon:28116"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: Bethesda"
                     /collection_date="1985-10-18"
     gene            1..1224
                     /gene="dcm"
                     /locus_tag="NXW64_28005"
     CDS             1..1224
                     /gene="dcm"
                     /locus_tag="NXW64_28005"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007367329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003886 - DNA (cytosine-5-)-methyltransferase activity
                     [Evidence IEA]; GO_process: GO:0006304 - DNA modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="UVO71731.1"
                     /translation="MRISDTNFADIMGVSKRTLLLWEQNGKFVPQKDNKGNKFFNTDDL
                     KGVPEIKEMIESSWNEELEIKPLRRYTSIELFAGGGGLALGMEKAGFHHLMLNEIDKYA
                     CETLKMNRPKWNVINDDIHNIDFKEYRNTVDLLTGGFPCQAFSYAGNKGGFEDTRGTLF
                     FELARAIQDIRPKVVFCENVKGLLSHDKGNTFNVIKNTINELGYTLVEPRILQAIKYKV
                     PQKRERLIIIAIRNDYANKVQFHWPSPYYKVMNLRDALYAGELYETDVPDSEGQKYPEK
                     KMKIMELVPMGGYWKDLPIDIQKEYMGGSFELEGGKTGMARRLSLDEPSLTLTCAPAQK
                     QTERCHPIETRPLTVREYARIQTFPDDWKFAGNMSAQYKQIGNAVPVNLSYAIGRSLIR
                     LLNDIEKL"
                     /name="dcm"
     gene            1706..1846
                     /locus_tag="NXW64_28010"
     CDS             1706..1846
                     /locus_tag="NXW64_28010"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVO71732.1"
                     /translation="MMKKIVIKIKWLLYKEQEKQYPILSFILDNIDDIDSIIKNILDCF
                     L"
                     /name="hypothetical protein"
     gene            complement(1929..3584)
                     /locus_tag="NXW64_28015"
     CDS             complement(1929..3584)
                     /locus_tag="NXW64_28015"
                     /inference="COORDINATES: protein motif:HMM:NF021897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="UVO71733.1"
                     /translation="MEQRKDIMTGIIRILILFIVGSIFLAIAGYILSFVIDIIGRIIVF
                     VLQWAVFGGIAWYGYKYFKKPKPKEKPAEVIPEEAIRLYTSSGQPIVIGNPFRGIFVLG
                     AAGSGKSESIAVPLLSEFIRNGFSGLVYDFKFPTLGQDVQSFISANGNEIKHHYIDLNN
                     PEQSPRVNPIQPDYLLNTSYAREFAQAIVANLIKESIKKPDFWNRSATDLLTACIWFLK
                     EEYPAMCDIPHVCVLVSSKDEDLLRLLQKNPQTAQMTMSIFSAMERGADGQVSGVVGTL
                     QSAIAQINTPELMYIFGGNDFSLDLNNPSSPAIVTVGTYPTLVQTFAPLCSLIITVATK
                     LMNQPGKVPSFVMIDEAPTVYIPGLDILPNTGRSNKISTVLMCQDLAQLSDGYGKEKAD
                     VLFASCNTHFYGRVSSSITAERLSRQFGKEDKVYTTASQSRTGFHRTSGTSESVQERDA
                     AKPSSFLSLEPGEFYGIAVESNVPFFRKKFRQAERPIPAQLEKPSHAESIQEYTLKVRE
                     DIKSILYGLKPDEPEEHQEPDIPSDIKFKGFSVD"
                     /name="TraM recognition domain-containing protein"
     gene            complement(3614..4513)
                     /locus_tag="NXW64_28020"
     CDS             complement(3614..4513)
                     /locus_tag="NXW64_28020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5712 family protein"
                     /protein_id="UVO71734.1"
                     /translation="MNIKIQGGGSGIYANTGSCTGVTTYLQHEDLERMKNGQEVQPFFN
                     NSRDYISAQEVTFKIDNNKAKLSRNDAKFYVITVSPSSRELEKMGKTEKEQAEAMRKYV
                     RDDVMQHYAEGFGKGLNKEDIEYYGKIHFERKGADRYDMHAHIIVSRKDRSNTRKLSPK
                     TNHTGKKNCGNVKGGFDRTDFFRKCESSFDKRTGYDRAPEQTFDYLNTMKNGSPKEIFQ
                     KKEWAERVNHERLEKMKAEWSRDLQEPHQEQGREESQQQGNSISQVPEINQAPQRKKQQ
                     EEELDQPRKRSRGFGMGM"
                     /name="DUF5712 family protein"
     gene            complement(4510..5127)
                     /locus_tag="NXW64_28025"
     CDS             complement(4510..5127)
                     /locus_tag="NXW64_28025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clindamycin resistance transfer factor btgA"
                     /protein_id="UVO71725.1"
                     /translation="MEKDSKTTVAVEPVAVERTTFTKLDRLAKANNVSKMEFLTHAINY
                     FEKYGINPVEHESPAQEMQKLIKRMDQVFAFLKKQETDLVRPACEALAGASTQITISLS
                     SLLSEEKFRRFLKENSELFIEVMNQSSEKGKAIDRAERAVRDSDATIRKNQQAIFKRIE
                     ANETAQLKAMQHIVSFLDAKGKTGLLDDINKVYEREKERRGL"
                     /name="clindamycin resistance transfer factor btgA"
     gene            6202..6942
                     /locus_tag="NXW64_28030"
     CDS             6202..6942
                     /locus_tag="NXW64_28030"
                     /inference="COORDINATES: protein motif:HMM:NF013235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UVO71726.1"
                     /translation="MANDEDENYTRVKKALIALRSKTFEFDDGRVWKLIGIIEKPQFNY
                     KRGWVKFEIQPEIYNAVLNFSKGFRKYELKTAMEFTSQYSMRFYELMSGQERPLIYSIE
                     DLKIMFGVQDKYKRNPDFIKRIVIPAKEELDAKSPYSFEYKVLKDGRSFHSLKLYPKYQ
                     PEHRDEELEKHDLQKQVSLGWDLDRLIRNYLKQELLFTDQEIRNNIDLFKVAQKELDLM
                     LELSTLKGKSRDKKESQRLYNKRT"
                     /name="replication initiation protein"
     gene            7002..7190
                     /locus_tag="NXW64_28035"
     CDS             7002..7190
                     /locus_tag="NXW64_28035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013622867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVO71727.1"
                     /translation="MKEGDLVMVSAEAVGLGKPMEAIIDKIETFMGQTLVTVTYTQPDA
                     LSGFGGCFVDAHITPKK"
                     /name="hypothetical protein"
     gene            7195..7548
                     /locus_tag="NXW64_28040"
     CDS             7195..7548
                     /locus_tag="NXW64_28040"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVO71728.1"
                     /translation="MSSALDKLNTFIERFRHDYTIGKIDIGKYGAYLKWKFEDSPLPSF
                     YEVVDMSEKVSEDLEKYDIKYPGAASYTEPYLNDDPWQSYKMYEDDKYIVSYLSYMENE
                     LLNLQVSGLLSRN"
                     /name="hypothetical protein"
     gene            7622..8122
                     /locus_tag="NXW64_28045"
     CDS             7622..8122
                     /locus_tag="NXW64_28045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007844266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVO71729.1"
                     /translation="MQEKTNIQTSTLRVPKNILEEIKIYCRKAGKPVGEWVETAWKFIE
                     KNDFDIYDKETTPFLPVPPDIEKERNQVEALCMLMSEFITAQKQIQLPAPELIAKAAEE
                     KVRAEMKSEEQAKELQVLQEENNRLRNEIKVLQEYKEKAHRELCRVRDEQKTIGKIKVN
                     TDI"
                     /name="hypothetical protein"
     gene            8124..8879
                     /locus_tag="NXW64_28050"
     CDS             8124..8879
                     /locus_tag="NXW64_28050"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004380877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]; GO_function:
                     GO:0009036 - type II site-specific deoxyribonuclease
                     activity [Evidence IEA]; GO_process: GO:0009307 - DNA
                     restriction-modification system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco47II family restriction endonuclease"
                     /protein_id="UVO71730.1"
                     /translation="MRNYNLGFISDELIFNHVKATMLQYSRKIDLKSFNKNIIDPIKLT
                     FDSKIYGQTIEEAITAECMRQIDKTNNNRIGYFHQYIFKHAGNNWVVPDNGQDGGFDII
                     NDNLHIYVEMKNKHNTMNSASASSTYIKMQHKILEDDQATCMLVEVIAKRSGNDTWNIT
                     IDENGRKRQYANNRIRKVSIDKFYEIVYNDKHAFLKLCKALPMILDDVLEEEPSAKLVN
                     TVYDELDKTDFYKSLYLLAFSTYEGFNEF"
                     /name="Eco47II family restriction endonuclease"
     Typing          5992..6972
                     /name="rep_cluster_1085"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.756"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3618..4513)
                     /name="MOBB"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.328"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagaataa gcgatactaa ttttgccgat ataatgggtg tatctaaaag gactttatta
       61 ttgtgggaac aaaacgggaa atttgttcca caaaaagata ataaaggtaa taagttcttt
      121 aacaccgatg atttgaaagg agttccagaa ataaaggaaa tgatagaatc aagctggaat
      181 gaagaacttg aaatcaagcc tttaaggaga tacacaagta tagaactttt tgctggtggc
      241 ggtggtttgg ctcttggcat ggaaaaagcc ggattccacc atttaatgct aaatgaaata
      301 gataaatatg cctgtgagac attaaaaatg aacagaccca aatggaatgt tataaatgat
      361 gatattcata atatagattt caaagaatac agaaatacag tagatctttt aacaggtgga
      421 ttcccttgtc aggcatttag ttatgctggg aataaaggag gtttcgaaga tacaagaggt
      481 actttgtttt ttgaattagc aagagcaata caagatataa gacctaaagt cgttttctgc
      541 gagaatgtaa aaggattact ttcacatgac aaagggaaca cgttcaatgt tataaaaaat
      601 actatcaatg aattagggta cacacttgta gaacctcgta ttcttcaagc tataaaatac
      661 aaagttccac aaaagagaga acgattaatt attatagcaa taagaaatga ctatgctaat
      721 aaagtacaat ttcattggcc ttctccatat tataaagtta tgaatcttcg tgatgcctta
      781 tatgctggag aactatatga aaccgatgtc ccagattctg aaggtcaaaa atatccagaa
      841 aagaaaatga aaataatgga gttagttcca atgggaggat attggaaaga tctccctatt
      901 gacattcaaa aggagtacat gggtggaagc tttgaattag aagggggcaa aacaggtatg
      961 gccagaaggt tatcgttaga tgaaccctct ttgacattaa catgcgctcc ggcacagaaa
     1021 caaacagaaa gatgtcatcc tattgaaaca aggccattaa cagttaggga atatgcaaga
     1081 atacagacat tcccagacga ttggaaattt gctggaaata tgtcagcaca atataaacaa
     1141 atagggaatg cagtaccagt taatttatct tatgcaatag gacgttcact aataaggtta
     1201 ctaaatgaca tagagaaact ctaaagaaaa atagctgtag aagaagtaaa atctacagct
     1261 attttttttg tccctacctc gccaagcaat ttttcttcat ttgcaaacct ttagagaggc
     1321 tacaagctta aaaaactgaa ggtttaagca aatgaaatca gacttcaaaa atttatttta
     1381 tcaattaatg gtatatgaaa atatcattga atacacatta gaaaatatag atgatatcat
     1441 tgaataccca catcttgtat tattctgtat aaatattgct ttacttattg tatatacacc
     1501 tttaatcatt caagagaaaa gaaggaagta cattagattg tagtacctat taatcaggca
     1561 tttacataag ctaaattttt gtcctttgaa atatgaaatt caaaatctaa cttcgcagaa
     1621 gcagaagcag aaagcagttt gatttcaaag ctttttggga gtagtggcct ttaacaatcc
     1681 actacatagg acaaaagcta tttttatgat gaagaaaata gttataaaaa tcaaatggct
     1741 tctgtataaa gaacaagaaa aacaatatcc aattctttct tttattcttg ataatataga
     1801 tgatattgac agcattataa aaaacatttt agattgtttt ctttagaagt atgcggtggc
     1861 tttatccacc gcataaatac cgttcagaaa acaaatttgg caaatacaga agtgtaaaag
     1921 tgtcccggtt agtcaacgga aaagcctttg aacttaatat ctgaagggat atccggttcc
     1981 tgatgttctt ccggttcatc aggcttcagc ccgtaaagta tagactttat atcctccctt
     2041 accttcagag tgtattcctg tatgctttcg gcatggctcg gtttctccag ctgtgccggg
     2101 attggccgtt ctgcctgtct gaacttcttt cggaaaaacg gcacattgct ttccaccgct
     2161 ataccgtaaa actccccagg ctccagagaa aggaagctgg aaggtttggc tgcatccctt
     2221 tcctgtactg attcgctggt tcctgaagtc cggtgaaagc ctgttctcga ttgtgatgcg
     2281 gttgtataga ctttatcttc cttcccgaat tggcgagaaa gtctttctgc cgttatgctc
     2341 gaagataccc taccataaaa gtgggtatta caggacgcaa acagcacatc ggccttttcc
     2401 ttgccgtaac cgtcagaaag ctgtgcaaga tcctggcaca tcagaactgt agatatttta
     2461 ttgcttcgtc ccgtgttcgg caatatgtca agaccaggta tatatacagt cggagcttcg
     2521 tcgatcatta caaaagaagg aactttgccg ggttggttca tcagctttgt ggccactgtt
     2581 ataataagac tgcataacgg tgcaaaggtc tgtacaaggg taggatatgt ccctacggtt
     2641 acaatagccg gagaagaagg attgttcaga tccagagaaa aatcatttcc accaaaaata
     2701 tacatcagtt ccggagtgtt tatctgtgct attgcagact gcaaagttcc tacaactccg
     2761 ctgacttgtc cgtctgctcc cctctccatt gcactgaaaa tactcattgt catttgtgcg
     2821 gtttgcggat tcttctgcaa aagccggaga agatcctcgt ccttactcga taccaaaacg
     2881 cacacatgag gtatatcgca catggccggg tattcttctt tcaggaacca gatacaggca
     2941 gttagaaggt ctgttgccga cctgttccag aaatcaggtt tctttatact ctcttttatc
     3001 aggttggcca caatagcctg tgcaaactct ctggcatagc ttgtattcaa caaataatct
     3061 ggctgtatag gatttactct cggtgactgt tccgggttat tcaagtctat ataatggtgc
     3121 tttatctcat tcccgtttgc actgataaaa gactgtacat cttgccccaa agtagggaac
     3181 ttaaaatcat atacaagacc ggaaaaaccg tttctgataa actctgacaa taacggcaca
     3241 gctatacttt cagactttcc agatccggca gcacccaaaa caaaaatacc cctgaaaggg
     3301 tttccgataa caatcggctg tcctgaagat gtatataacc gtatagcttc ttcaggtatt
     3361 acttcagctg gtttctcctt cggtttaggt ttcttgaaat acttataacc ataccaggca
     3421 ataccaccga aaacagccca ctgaagtaca aagactataa tacggccaat aatatcaatg
     3481 acaaaggaaa ggatatatcc ggctatggcc agaaatatac tacctacaat aaacagaatt
     3541 aagattctga taatacctgt cattatgtcc tttctttgtt ccatacgctt ttgattttgt
     3601 tacataatca aatttacata cccattccaa aaccccggct tcttttcctg ggctggtcga
     3661 gttcttcctc ctgttgcttc ttccgctgcg gtgcctggtt gatttccgga acttgggata
     3721 tactgtttcc ctgttgttgg ctctcttccc ggccttgttc ctggtgcggt tcctgaagat
     3781 cccggctcca ttcggctttc atcttttcga gccgttcatg gttcacacgt tccgcccatt
     3841 ctttcttctg gaagatttct ttcgggcttc cgttcttcat ggtattgaga tagtcgaagg
     3901 tctgttccgg agcccggtca taccctgtgc gcttgtcaaa tgaagattca cactttcgga
     3961 aaaaatctgt cctgtcaaaa cctcccttca cgtttccgca gttcttcttc ccggtgtggt
     4021 tggtctttgg gcttagcttc ctggtgttgc tcctgtcctt ccggctgacg atgatatgtg
     4081 cgtgcatgtc gtaccggtcg gctcccttcc tctcgaaatg gatttttccg taatactcga
     4141 tatcctcttt gttcaagcct tttccgaacc cttccgcata gtgctgcatc acatcatcac
     4201 ggacatactt tctcatggct tcagcctgtt ccttctcggt cttgcccatt ttctccagct
     4261 cccggctgga agggcttacc gtgatcacat agaacttcgc atcgttccgg cttaattttg
     4321 ccttgttgtt gtctatctta aaggttactt cctgtgcgct tatatagtca cgtgagttat
     4381 tgaaaaacgg ctgcacttcc tggccgttct tcatgcgctc cagatcctca tgctggaggt
     4441 aggtcgttac tcctgtgcag ctccctgtat tggcatagat cccggaaccg ccaccctgaa
     4501 tttttatatt catagtcccc tcctttcctt ttccctctca tagaccttgt ttatatcatc
     4561 gagcagcccg gtctttcctt ttgcatcgag gaaggaaact atatgttgca tggctttaag
     4621 ttgtgctgtt tcgttggctt ctatccgctt gaaaatggcc tgctggttct tcctgatagt
     4681 tgcatcagaa tcccgtaccg ctctttcagc tctgtcaata gcttttccct tttcgctgga
     4741 ttggttcatc acctcgatga aaagttcgct gttctccttc aggaaccgcc tgaacttttc
     4801 ttccgaaagt aaggatgaaa ggcttatggt tatctgtgtg ctggctccgg ccagagcttc
     4861 acaagccgga cgcacaagat ccgtttcctg tttcttcagg aatgcgaaca cctgatccat
     4921 gcgcttgata agcttctgca tttcctgtgc cgggctttca tgctcgaccg ggtttatacc
     4981 gtacttctca aaatagttta tagcatgtgt aaggaactcc atttttgaca cattgtttgc
     5041 cttggccaga cggtcaagtt tagtaaaagt agtacgttct actgctaccg gttctactgc
     5101 taccgttgtt ttgctgtctt tttccatgat attataaggc ttattataat gaattaatat
     5161 actaacttat tggtttacaa gatagtatat ttattcatta taaccaaatt atattagtat
     5221 aatttcctaa ataattgatt tacagctaaa aaccccgtag ggcaagagga aaaaacaggg
     5281 cttgcccggt ttcctcttac tgttctatcg aacaccctcc ccagcgcgaa attagcaaag
     5341 gggacgggga gagatagaac ctatcccaca cacgtgaaca cacacgaaaa ccgcacacct
     5401 ggtacacaac agaattacct aacaaaaccg cacaacagaa cgtgtccgga acaccacaaa
     5461 aaattcatcg gtcggggagc gccgcacggc aaggggcggg accacccgtc ccgacgagtg
     5521 cagccgtttg ctatgttttg attgtgtcac aaaatcaaaa gaagcgccaa atataaacac
     5581 atatatacaa aataacacac attttaacgc gccagaaaat tcgatccttt taaaacgctg
     5641 cgctggcttt tgccttgctt tcaacaacat tcaaaaaatc gtttttcacg gattgggagg
     5701 tttagaagcc acattccggc taaaatttta tgacttgttt ttaatcatag cttgttatat
     5761 gcttatcaaa ctctaaaaaa agattgcaaa gtgttattat tcagcttgat acacaaataa
     5821 aaactaacct aaaaacgaca tattgaatgt tataaacgac atattgaatg ttttttttct
     5881 ccttaaaaac gacatgttga atgtttttga cgacatatat ttttaaatgt cgtttttatt
     5941 tcatacattt gtgaacattc attatgtcgt tttttaaata acaaagttat atggctatac
     6001 gaataacaaa taaagacgtg atacagagct atttaatgac tgtatctaag tataattttt
     6061 ccgtatatga aaaacgcata gtttatagac ttgttgaagc tatgcagtgt gaaatagaag
     6121 gggaaaaaac tataccctgg gcttcggata gaaaagaatc tatatgatga tcgggtcgta
     6181 tccatgccta tatctgcgtt tttggctaat gacgaagacg aaaattacac ccgtgttaag
     6241 aaagctctta tagcactaag aagcaagacc tttgaatttg acgatggccg agtttggaag
     6301 ctgattggta ttatagaaaa gccgcaattc aactacaaaa gaggatgggt taagtttgag
     6361 atacaaccgg aaatatataa tgccgttctt aacttctcaa aaggcttcag gaagtatgaa
     6421 cttaaaacgg ccatggagtt tacaagtcag tactcgatgc gtttctatga actcatgagt
     6481 ggtcaagaaa ggcctttgat ttacagtata gaggatctga aaatcatgtt tggtgtacag
     6541 gacaaataca aacgtaaccc ggatttcata aaaagaatag taataccagc aaaagaagaa
     6601 ctggatgcca aaagcccata ttcatttgaa tacaaagtct tgaaagacgg ccgttctttt
     6661 cactcactaa agttgtaccc aaaatatcag ccggaacaca gggatgaaga actcgaaaag
     6721 cacgatttac aaaagcaagt tagtttaggt tgggacttag acagacttat ccgtaattat
     6781 ctaaaacaag aactactgtt cacggatcag gagataagaa acaatataga tttgtttaag
     6841 gtagcacaaa aagaacttga tttaatgctg gagctttcaa ctttgaaagg taaatcaaga
     6901 gataaaaaag aatcccaaag gctatataat aaacgcactt aagggaaaag taagagataa
     6961 aggataattt gattctgtca cacaatcaaa aaaattaaga tatgaaagaa ggtgatttgg
     7021 taatggtttc agcagaagcc gtaggcttag ggaaaccaat ggaagccata atagacaaga
     7081 ttgaaacatt catggggcaa acccttgtaa cagtcactta tacgcaacca gacgctttat
     7141 ctggcttcgg tggttgtttt gtagatgcac atataacccc aaaaaaatag tatcatgagt
     7201 agtgctttag ataaactaaa tacattcata gaaagattca gacatgacta tactatcgga
     7261 aaaatagata taggcaaata cggtgcatat ctgaaatgga aatttgagga ttctcctttg
     7321 ccttctttct atgaggtagt agatatgtca gaaaaggttt ctgaggatct ggagaaatac
     7381 gacattaaat atcctggagc agcttcatat actgaaccat acctgaatga tgatccatgg
     7441 caaagctaca agatgtatga agatgataaa tatatcgtat catatcttag ctatatggaa
     7501 aatgaactgc ttaatcttca ggtaagtggg ctattatcca ggaattaacc taaatatatt
     7561 ttttgtagaa agtgtatatt gataaatata ttctttatat ttttgcacat aaatcattga
     7621 tatgcaagaa aaaacaaata tacagacatc aactctaaga gtacctaaaa acatactcga
     7681 agaaataaaa atatattgcc gaaaagccgg aaagcctgtc ggtgaatggg ttgaaacagc
     7741 ctggaaattc atagagaaga atgacttcga catctatgac aaggaaacaa cgccatttct
     7801 gcctgttcct ccagacatcg agaaggaaag gaaccaggta gaagccttgt gtatgcttat
     7861 gagtgaattt ataactgcgc agaagcagat acagcttccg gctccggaac ttatcgcaaa
     7921 ggcagctgaa gaaaaagtac gtgctgaaat gaagtctgaa gaacaggcaa aagaactcca
     7981 ggttcttcag gaagaaaaca accgattgcg taacgaaatc aaagttcttc aggagtataa
     8041 ggaaaaggca caccgggaac tatgccgggt acgtgacgaa cagaaaacaa tagggaaaat
     8101 aaaagtaaat acagatatat aatatgagaa attataattt aggattcatc tctgatgaat
     8161 taatcttcaa tcacgtaaag gctacaatgt tgcaatacag ccgtaaaata gacttaaagt
     8221 cattcaacaa gaatataata gatcctataa agctgacttt cgattcgaaa atttatggcc
     8281 aaactataga agaagctata acagccgaat gtatgcgcca gattgataag accaataata
     8341 acagaatagg ttattttcat cagtacatat ttaagcatgc aggcaataac tgggttgttc
     8401 ctgataacgg ccaggatggt ggatttgaca ttataaatga caatctacat atatatgtag
     8461 aaatgaagaa caaacataac accatgaatt cggcaagtgc atcaagtaca tacataaaga
     8521 tgcaacataa gatattagag gatgatcagg caacatgtat gcttgttgaa gttattgcta
     8581 agagatccgg aaacgacacc tggaatataa ccattgacga aaacggaaga aaaagacaat
     8641 atgctaataa tagaattagg aaagtatcaa tagacaaatt ctatgaaata gtatataatg
     8701 acaaacatgc gttcttaaag ctttgtaaag cattgcctat gatattggac gatgttctcg
     8761 aggaggagcc ttcagcaaaa cttgtaaata ccgtatatga cgagctcgat aaaactgact
     8821 tttataagag tctgtatctt cttgcattta gtacttacga agggtttaat gagttttaag
     8881 a
//
