LOCUS       CP103106                4101 bp    DNA     circular BCT 29-AUG-2022
DEFINITION  Bacteroides thetaiotaomicron strain BFG-446 plasmid unnamed2,
            complete sequence.
ACCESSION   CP103106
VERSION     CP103106.1
DBLINK      BioProject: PRJNA646575
            BioSample: SAMN30323354
KEYWORDS    .
SOURCE      Bacteroides thetaiotaomicron
  ORGANISM  Bacteroides thetaiotaomicron
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Genome Sequencing of Bacteroides fragilis Group Isolates with
            Nanopore Technology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Tisza,M.J., Smith,D. and Dekker,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2022) LCIM, National Institute of Allergy and
            Infectious Diseases, 9000 Rockville Pike, Bethesda, MD 20892, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 53x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/22/2022 19:44:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,322
            CDSs (total)                      :: 5,225
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 97
            rRNAs                             :: 4, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 80
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 690
            CDSs (without protein)            :: 690
            Pseudo Genes (ambiguous residues) :: 0 of 690
            Pseudo Genes (frameshifted)       :: 632 of 690
            Pseudo Genes (incomplete)         :: 68 of 690
            Pseudo Genes (internal stop)      :: 20 of 690
            Pseudo Genes (multiple problems)  :: 25 of 690
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Bacteroides thetaiotaomicron"
                     /mol_type="genomic DNA"
                     /strain="BFG-446"
                     /host="Homo sapiens"
                     /db_xref="taxon:818"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: Bethesda"
                     /collection_date="2001-01-09"
     gene            1..1026
                     /locus_tag="NXW43_26570"
     CDS             1..1026
                     /locus_tag="NXW43_26570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008657007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UVO76265.1"
                     /translation="MKKKLPITKNKDVVVSWVYTWSKQQDMSIHEQRIVLRILEACQAE
                     LKGVKLKDYAGTKRKFEHGLWDVDAQMHVSDVIFSGRDYNEIIAALDSLAGRFFTYEDD
                     EEWWKCGFISNPKYKKRTGIITFRVSNDLWDVFTKFAKGYREFELNKALALPTGYSLRF
                     YMLMSGQVYPLDISLENLKDRLGIPADKYKDKNGKDRIDHFEERVLKPAKAALDESCPY
                     TFNYVKVRENPNNKRSKVTGFRFYPVYQPQFRDEELEGKELQAKVTARYQIDSHVYEYL
                     RYSCGFTSEEINRNKETFITAQEKITDLIGELALLNGKSREKNNPKGWIINALKGKIKD
                     K"
                     /name="replication initiation protein"
     gene            complement(1063..1293)
                     /locus_tag="NXW43_26575"
     CDS             complement(1063..1293)
                     /locus_tag="NXW43_26575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005683050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="charged multivesicular body protein 2b-B"
                     /protein_id="UVO76266.1"
                     /translation="MGTTEHEEPRFFFILNKGAKSGGEITHAVLNGSIVSKPAGWDAFH
                     GLALAREKLSSEEIQQQMKELGVEMEIVPLI"
                     /name="charged multivesicular body protein 2b-B"
     gene            complement(1313..1765)
                     /locus_tag="NXW43_26580"
     CDS             complement(1313..1765)
                     /locus_tag="NXW43_26580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVO76267.1"
                     /translation="METNSHPEAAASNVKQGQYSPRKRKDTTPRVNPQLAVELVYQELK
                     RVEVYTKRIEDATARKVQIDGKSLESAENRLKNVLADFERQGYRMKNGGYVDKRISFYS
                     ILCAVISLLFACFMCYLWTDAAKDRDNYKQYYEYYQEQAREQKGNK"
                     /name="hypothetical protein"
     gene            complement(1722..2473)
                     /locus_tag="NXW43_26585"
                     /pseudo=""
     CDS             complement(1722..2473)
                     /locus_tag="NXW43_26585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560590.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2962..3156)
                     /locus_tag="NXW43_26590"
     CDS             complement(2962..3156)
                     /locus_tag="NXW43_26590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVO76262.1"
                     /translation="MVEYCVYWLENGEPMHEVFSSLAAEMYSCAIRGKENIEWVEVSEE
                     ETIDLDELEDMFPDDFCGV"
                     /name="hypothetical protein"
     gene            complement(3167..3442)
                     /locus_tag="NXW43_26595"
     CDS             complement(3167..3442)
                     /locus_tag="NXW43_26595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004519 - endonuclease activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YoeB family toxin"
                     /protein_id="UVO76263.1"
                     /translation="MMYTIRVSDGVDKVIAKWKKSNPNLFKKYKKIYKELLEHPKTGLG
                     HPEALRGGGDITWSRHITAHDRIIYDIYEEVVEVYILEVEGHYNDK"
                     /name="type II toxin-antitoxin system YoeB family toxin"
     gene            complement(3439..3690)
                     /locus_tag="NXW43_26600"
     CDS             complement(3439..3690)
                     /locus_tag="NXW43_26600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UVO76264.1"
                     /translation="MEALSVREYRNNLAASFTKADNGEQVLIRRKNEIYALVKVGREDL
                     MITPELQARIDKAREEIKSGKCVTLKSSEDIDAYFDSL"
                     /name="hypothetical protein"
     Typing          complement(1998..2473)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="83.648"
                     /coverage="97.0"
                     /evalue="1.78e-108"
ORIGIN
        1 atgaaaaaga aactacctat taccaagaat aaagatgttg ttgtttcatg ggtatataca
       61 tggtcaaaac agcaagacat gtccatacac gaacaaagaa tagttcttcg catactggaa
      121 gcgtgccaag ctgaactaaa gggagtaaaa ctgaaagatt atgcaggcac gaaacgcaag
      181 tttgagcatg gactttggga tgttgatgca caaatgcatg tatctgacgt tattttttcc
      241 ggacgtgatt acaatgaaat catagccgca ctcgattctt tggcaggacg tttttttact
      301 tacgaagatg acgaggaatg gtggaagtgc ggatttatat ctaacccaaa atataaaaaa
      361 cgcactggca ttataacctt tcgtgtgtct aacgaccttt gggatgtgtt taccaagttc
      421 gccaaggggt acagagaatt tgagttaaac aaggctcttg cgctacctac tgggtattcc
      481 cttaggtttt acatgttaat gagtgggcag gtgtaccctt tggatatatc ccttgaaaac
      541 ttgaaagacc gtcttggcat acctgcggac aaatacaagg acaagaacgg aaaagacaga
      601 atagatcatt ttgaggaaag agttttgaaa ccagcaaagg ctgcgcttga tgagagctgc
      661 ccatacactt tcaactacgt gaaagtgcgt gaaaacccaa acaacaaacg tagcaaggtg
      721 accgggttta ggttctaccc ggtctatcaa ccacagttca gagatgaaga actggaaggg
      781 aaagaattgc aagcaaaggt tacagcacga tatcagatag acagccatgt gtacgagtac
      841 ctccgttatt cctgcggctt cacatcggaa gagataaacc ggaacaaaga gacattcata
      901 acggcacaag aaaagataac cgaccttatc ggagaactgg ctctcctaaa cggaaaatca
      961 cgagaaaaga acaatccgaa agggtggatt ataaacgccc ttaaagggaa aatcaaggat
     1021 aagtaacaag attacccggc tgaaacactc cgggtaatct tgtcaaatca gaggaacgat
     1081 ttccatttct acaccaagtt ctttcatctg ctgttgtatt tcctcgctgc ttagtttctc
     1141 tctcgccagt gcaagcccat gaaaggcgtc ccagcctgcc ggcttcgaaa cgatactacc
     1201 gtttaggacg gcatgggtga tttcgccgcc ggacttcgca cccttgttca gtatgaagaa
     1261 gaaccggggt tcttcgtgtt ccgttgttcc cataattcgt aaagttagat gtttatttat
     1321 tccccttttg ttcccttgcc tgctcttggt agtattcata atactgcttg tagttgtccc
     1381 ggtctttggc tgcatccgtc caaagatagc acatgaaaca agcgaacaat agggagataa
     1441 cagcacaaag gattgagtag aacgaaattc gtttgtccac ataaccgccg tttttcattc
     1501 tgtacccctg ccgttcaaag tccgccaaca cgttttttag gcgattttcg gcactttcaa
     1561 ggcttttccc gtctatttgt acctttcggg ctgttgcgtc ctctatgcgc ttcgtataga
     1621 cttcaacacg ctttagttct tgatacacca gttctaccgc aagctgcggg tttacccttg
     1681 gcgttgtatc ttttcttttc cgggggctgt actgtccctg cttaacgttg gaagctgccg
     1741 cttccgggtg tgagtttgtt tccatgagat atgttcattg agttgcactt cagtttgctt
     1801 tgtgtccgct cgatatgggc gagagtgtag cctttcccta tctcgcttgc cttatactcc
     1861 gtaccgcttc gggctgtgac atagtagccg ttaagtttcc cggtgcttgc cctggcttcc
     1921 cggactttaa aacctctctt accaagttct tccttgaatt tggtaaagtc aaagccctgc
     1981 atcttcttca atacgcgtcc tgcgactgta caaagttccg ttccttggct atggcattgg
     2041 cggcttccgt tgcctttttc cctatccagt tgtcccggta cagttccccg gaaagggaaa
     2101 cccggtttgc cagtatgtgc aggtgggctt gctctttctt gctctccgta ccgctgtgcc
     2161 ggatgatgat gtactggtgg ttcgccaacc ccatgcgctg catgaagtcg ttaccaagtt
     2221 ccgcccagtc cgcatccgta aaggtggcgc tttcctctat cgaggggctg acctcgaaac
     2281 gtaggcagtt gttttttacg ttcggaaaat caatgtgata cggtttcatt tcctgcacca
     2341 tttccgcacc agtacaccca taaagttcat gccgggctat ctcggtggct actgcctgcc
     2401 cgtttatctc tttggcgagg tcgtactcca atgccgccac accatgtgat atgcttttcc
     2461 ctttccctat catcgtgaga gtattttttt gagctctgta attagtgccc ggttctcctc
     2521 gaacacttcc cggtactggc gaccaccgaa gtagttgttc agccgttgga gcgttccttt
     2581 caactgggct atgtcccgga gaagctgccg ttcctcctcg gtgtacctct cccttgggcg
     2641 ccccccaagg gacaaactcc ggcagtattc cgatacgctt agactgcacc gggctgcctg
     2701 ctccgcaagt gcgtcctttt caagttcggt acaccggaat gtaatctttt cgttccgtct
     2761 tagttccatg ccacaaaggt aatcttaaaa tctttttgag caaagcgaga aaagcaagaa
     2821 ttgtccgaca aggacacttc ttgctatata tacacatgcc gcatgggaat acatccacgc
     2881 caaccgccca gcaaaaagcc ccggcgtgtt agaaaatccc ccacagccta acaaattcta
     2941 ccccatttct acccaagggg attacacgcc gcagaagtca tccgggaaca tgtcttccag
     3001 ttcgtccaaa tcaatggttt cttcttcgga cacctccacc cattcaatgt tttctttccc
     3061 tcttatcgca catgagtaca tctcggcggc aagagaagag aacacctcgt gcataggctc
     3121 gccgttctct aaccagtaaa cacaatattc gaccataaca gttttatcat ttatcgttgt
     3181 aatgcccctc tacctccaag atgtagacct ctaccacttc ctcgtaaatg tcatagatta
     3241 tccggtcatg ggcggtaatg tgtcgagacc atgtaatatc cccaccgccc cttaacgctt
     3301 cgggatgccc aagcccagtc ttagggtgct ctaacaattc tttgtagatt ttcttatact
     3361 tcttgaacaa attggggttt gatttcttcc atttggcaat caccttatcc accccgtcgg
     3421 aaaccctaat tgtgtacatc atagactatc gaaataagcg tcaatatcct cactgctttt
     3481 gagggtaaca cactttccgg atttgatttc ttcccttgcc ttgtcaatcc ttgcttgcag
     3541 ctccggggtt atcatcaaat cttcacgacc aacttttacc aaagcgtaaa tctcgttttt
     3601 cctgcgaatt agtacctgct cgccattgtc agctttggtg aaagacgctg cgaggttgtt
     3661 acggtattct cttactgata atgcttccat atctgtttgc ttttaaattc agcacaaaga
     3721 tagctatatt tcaataaaat acaaacattt tgtacacaaa cgtgtacacg ccataaaaac
     3781 ccgtttccaa tcctaccgcc cgttggttgg ttttgctttg ctctttttcc ctatcgtttt
     3841 tctttttccg acagtaaatc aaccacttag ctctataaat tccccttgtc tgttataagc
     3901 atcgtctgta atatgctcta tcacttcttc gcaaacgccc ataaataaag ggattagaac
     3961 atgtgtttcc ccacactttg ggagtttagt ccccacactt tgggagttta aaagtaaatt
     4021 agtccccaca ctttgggagt tttacccctc ttttttgttt taatgtgggg attgtgtata
     4081 tttgcagcaa atcaatcgtt t
//
