LOCUS       CP102662                2362 bp    DNA     circular BCT 21-AUG-2022
DEFINITION  Staphylococcus haemolyticus strain 4M plasmid unnamed4, complete
            sequence.
ACCESSION   CP102662
VERSION     CP102662.1
DBLINK      BioProject: PRJNA865942
            BioSample: SAMN30148943
KEYWORDS    .
SOURCE      Staphylococcus haemolyticus
  ORGANISM  Staphylococcus haemolyticus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Brovko,F. and Sokolov,S.
  TITLE     Identification and pathogenic (toxigenic) potential of
            Staphylococcus haemolyticus and Bacillus paranthracis consortium
            isolated from bovine mastitis in Russia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Brovko,F. and Sokolov,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-AUG-2022) Laboratory of Immunochemistry, Shemyakin and
            Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy
            of Sciences, Miklukho-Maklaya, 16/10, Moscow 117997, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Flye v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/15/2022 09:54:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 2,597
            CDSs (total)                      :: 2,508
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 89
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 20 of 45
            Pseudo Genes (incomplete)         :: 24 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from staphylococcus haemolyticus.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Staphylococcus haemolyticus"
                     /mol_type="genomic DNA"
                     /strain="4M"
                     /isolation_source="milk"
                     /host="bovine"
                     /db_xref="taxon:1283"
                     /plasmid="unnamed4"
                     /geo_loc_name="Russia: Moscow region"
                     /collection_date="2020"
     gene            complement(join(2321..2362,1..693))
                     /gene="erm(C)"
                     /locus_tag="NRZ53_12980"
     ARG             complement(2..693)
                     /name="erm(C)"
                     /gene="erm(C)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(C)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="94.67"
     gene            complement(1397..1873)
                     /locus_tag="NRZ53_12985"
     CDS             complement(1397..1873)
                     /locus_tag="NRZ53_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="UVD90853.1"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIVQ
                     LISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEEGN
                     IIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEKQENALSDYYSFKD"
                     /name="replication/maintenance protein RepL"
     ARG             complement(2..693)
                     /name="ErmC"
                     /gene="ErmC"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="99.57"
                     /coverage="94.67"
     Typing          complement(1388..1873)
                     /name="rep_cluster_1947"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.591"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttgttcaaag ctaatattgt ttaaatcgtc aattcctgca tgttttaagg aattgttaaa
       61 ttgatttttt gtaaatattt tcttgtattc tttgttaacc catttcataa cgaaataatt
      121 atacttctgt ttatctttgt gtgatattct tgattttttt ctatttaatc tgataagtga
      181 gctattcact ttaggtttag gatgaaaata ttctcttgga accatactta atatagaaat
      241 atcaacttct gccattaaaa ataatgccaa tgagcgtttt gtatttaata atcttttagc
      301 aaacccgtat tccacgatta aataaatctc atcagctata ctatcaaaaa caattttgcg
      361 tattatatcc gtacttatgt tataaggtat attaccaaat attttatagg attggttttt
      421 aggaaattta aactgcaata tatccttgtt taaaacttgg aaattatcgt gatcaacaag
      481 tttattttct gtagttttgc ataatttatg gtctatttca atggcagtta cgaaattaca
      541 cctctgtact aattcaaggg taaaatgccc ttttcctgag ccgatttcaa agatattatc
      601 atgttcattt aatcttatat ttgtcattat tttatctata ttatgttttg aagtaataaa
      661 gttttgactg tgttttatat ttttctcgtt cattataacc ctcttttttc ctccttataa
      721 aattagtata attatagcac gagctctgat aaatatgaac atgatgagtg atcgttaaat
      781 ttatactgca atctgatgcg attattgaat aaaagatatg agagatttat ctagtttctt
      841 tttttacaag aaaaaagaaa gttcttaaag gttttatact tttggtcgta gagcacacgg
      901 tttaacgact taattacgaa gtaaataagt ctagtgtgtt agactttaat gtttttttaa
      961 ggcattagtg catttaagcg tcagagcatg gctttatgcc gagaaaacta ttggttggaa
     1021 tggcgtgtgt gttagccaaa gctttggcga gttggttggg ggtttcatgg gattaatccc
     1081 atgaaagtac caactcaaca acacactaac gcctgttggt tccaaccaat aggaaattgg
     1141 aataagcaat tagtataatg agagtataat gttggtataa cgttagtata atgatgcttt
     1201 ttttcattat attttttatg tactttaaac ctgcacgctt atgtgaatta gaaaaagctt
     1261 aatcgcattt catagattga cctcccaata actacgtggt gttattggga ggtcaatcta
     1321 tttcatttgc gttaagcttt tgatctacat taagcgtgca ggttttttgt ctatttgtag
     1381 acgattttat gttatactag tccttgaaag aataataatc agataatgca ttttcttgtt
     1441 tttcatttgc ctcttgctca aagttcccaa attcgagtaa gaggtatttt tgtttttggt
     1501 cgtcgcctct cattagtagt tcagggttta acattaatac tccagttttt ctttttataa
     1561 tatttccttc ttctaagatt ttaagtgttg ttattactgt ttgtagactt gttcctgtag
     1621 cttttgctat ttctcttgtt gtagctatca ttgtattgtt acttaagtgg acattatcta
     1681 ggatatagtt aacgatttta agtttttttc cgccaatcat atctaacata cttattaatt
     1741 gcactatata tgcctttacg aagttaccag acgtttgttt acggtataac ttgtctacct
     1801 ctatgacttc tccactttct tcgtctatga gcctctgaga gcctttatag actgttccat
     1861 atctttcttt catctttttc tcactcctta ttttaaacta ttctaactat atcataactg
     1921 ttctaaaaaa aaaagaacat ttgttaaaag aaattagaac aaaatgagtg aaaaattaga
     1981 acaaacaaat tccttataaa ccttatcatc tcaacctata ttaagatttt acctagttga
     2041 atcttctttt ctatataaag cgtcggagca tatcaggggg ttatctaacg taaatgctac
     2101 ccttcggctc gctttcgctc ggcattgacg tcagatactg caccccctga acccccatgc
     2161 tccaacagca aaaaggaaac tttttgctgc ttttccgacg cttattcgct tcgctcatat
     2221 ttatatagaa aagaagtgaa tgcgcaaaag acataatcga ttcacaaaaa ataggtacac
     2281 gaaaaacaag ttaagggatg cataaactgc atcccttaac ttacttatta aataatttat
     2341 agctattgaa aagagataag aa
//
