LOCUS       CP102661                3048 bp    DNA     circular BCT 21-AUG-2022
DEFINITION  Staphylococcus haemolyticus strain 4M plasmid unnamed3, complete
            sequence.
ACCESSION   CP102661
VERSION     CP102661.1
DBLINK      BioProject: PRJNA865942
            BioSample: SAMN30148943
KEYWORDS    .
SOURCE      Staphylococcus haemolyticus
  ORGANISM  Staphylococcus haemolyticus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Brovko,F. and Sokolov,S.
  TITLE     Identification and pathogenic (toxigenic) potential of
            Staphylococcus haemolyticus and Bacillus paranthracis consortium
            isolated from bovine mastitis in Russia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Brovko,F. and Sokolov,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-AUG-2022) Laboratory of Immunochemistry, Shemyakin and
            Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy
            of Sciences, Miklukho-Maklaya, 16/10, Moscow 117997, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Flye v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/15/2022 09:54:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 2,597
            CDSs (total)                      :: 2,508
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 89
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 20 of 45
            Pseudo Genes (incomplete)         :: 24 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from staphylococcus haemolyticus.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Staphylococcus haemolyticus"
                     /mol_type="genomic DNA"
                     /strain="4M"
                     /isolation_source="milk"
                     /host="bovine"
                     /db_xref="taxon:1283"
                     /plasmid="unnamed3"
                     /geo_loc_name="Russia: Moscow region"
                     /collection_date="2020"
     gene            join(3031..3048,1..804)
                     /locus_tag="NRZ53_12970"
     CDS             join(3031..3048,1..804)
                     /locus_tag="NRZ53_12970"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="UVD90851.1"
                     /translation="MFFKCSICDEKLKRGYKTLACENNHTFDIAKEGYSNLISGHKKSK
                     YDKKLFIARRELNDSSNLYSILISKIIEIIKKYEIKTKNNVIDVGTGEGYILNEISKTF
                     LGENEFLGIDIAKEGVKKAAKSYKNINWLVADLADIPVMNGNVDFLINVLSPSNYDEFK
                     RVLDKEGKLIKIIPNSDYLKEIRNIVFSNHKNKDYDNSEVIDLFEANFNKIEMIDISYK
                     VKLNKEELNKLLDMTPLTWNLSDVEKEGIIKKNLSEITMSFKVLVGGFKTS"
                     /name="methyltransferase domain-containing protein"
     gene            987..1991
                     /locus_tag="NRZ53_12975"
     CDS             987..1991
                     /locus_tag="NRZ53_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020368559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="UVD90852.1"
                     /translation="MQYNTTKCIDENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNVLEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EEVVKEKPTARWLFLTLSTKNAIDGDHLEQSLKHMSKAFNKLKMYTKVKKNLVGFLRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQVEWVDLWQKALQVNYRPVANIKAI
                     KPNQKGDKDIQAAIKETSKYSVKSSDFLTDDDEKNQEIVNDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDAEDGNLINTSDEDKTTDEEEKAHSITAIWNFEKQNYYLKDLKR"
                     /name="protein rep"
     Typing          988..1991
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.806"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atatgtgatg aaaaattaaa gagaggttat aaaactttag catgtgagaa taaccatact
       61 tttgatattg caaaagaggg ttattcgaat ttaatttcag ggcataaaaa aagtaaatac
      121 gataaaaaat tgtttattgc tagaagagaa ttgaatgata gtagcaattt gtattcgata
      181 ttaatttcaa aaatcataga aattattaaa aaatatgaga ttaaaactaa aaataatgtg
      241 atagatgttg gtactggtga gggttatatt ttaaatgaaa ttagtaaaac atttcttggt
      301 gaaaatgaat tcttaggtat agacattgca aaagaaggtg taaaaaaagc agcaaaatca
      361 tataaaaata taaattggtt ggttgctgat ctagctgata taccagttat gaatgggaat
      421 gtagactttt taattaatgt gcttagccct tctaattatg atgagtttaa aagagttcta
      481 gataaagagg ggaaattgat aaaaataatt cctaatagtg attatttaaa agaaataagg
      541 aatatagtct ttagtaatca taaaaataag gattatgata attctgaggt tatagattta
      601 tttgaagcta attttaataa aattgaaatg atagacatta gttataaggt taaattaaat
      661 aaagaagaat tgaataaatt acttgatatg acgcctttaa cgtggaattt aagtgacgta
      721 gaaaaagaag gaataattaa gaaaaactta agtgaaatta cgatgagttt taaggttttg
      781 gttggtggtt ttaaaacgag ttgaaaacga gtttcttctt gtcttgatac tatatagaaa
      841 taactcaaag atataaaaag ttgctttatc gttggtatga cagtgttttt atgtattttt
      901 gtgcgtgaaa gaaaaaattg acataaaaaa gctccagtaa tatgattaag ttacgacgct
      961 taaatcaata ctggaggtct ttttatatgc aatataatac tactaaatgt atagacgaaa
     1021 atcaagataa taaaacatta aaagatatga cgaaaagtgg gaaacaacgc ccatggagag
     1081 aaaagaagat agataatgta agttatgcag atatactgga aatcttaaaa attaaaaagg
     1141 cttttaacgt aaaacaatgt ggtaacgtct tagaattcaa gccaactgat gaaggttatt
     1201 tgaaattaca taagacatgg ttttgtaagt caaaattatg cccagtttgt aattggaggc
     1261 gtgctatgaa aaatagttat caagctcaaa aagtgattga agaagtggtt aaagaaaagc
     1321 caactgctcg ttggttattt cttactttat cgactaaaaa tgcaatagac ggtgaccact
     1381 tagaacaaag tttaaaacac atgtctaagg cttttaataa attaaagatg tatacaaaag
     1441 ttaaaaagaa tttggtgggt tttttacgct caactgaagt aacagttaat aaaaatgacg
     1501 gtagttataa tcaacatatg cacgttttat tgtgtgttga aaatgcgtat ttcagaaaaa
     1561 aagaaaacta cattacgcaa gttgaatggg ttgatttatg gcaaaaagca ttacaggtta
     1621 attatcgacc agtagcaaat attaaagcga tcaaaccaaa tcaaaaaggc gataaagata
     1681 ttcaagcagc tatcaaagaa acctctaaat attcagttaa gtcatctgat tttttaactg
     1741 atgatgacga aaaaaatcaa gaaatcgtga atgatttgga aaaaggttta taccgaaaac
     1801 gtatgttgag ttatggtgga ttgcttaaac aaaaacataa aattttaaat ttagacgatg
     1861 ccgaagatgg caatttgatt aatacaagtg acgaagataa aacaacagac gaagaagaaa
     1921 aagcacattc aattacggca atttggaatt ttgaaaaaca aaattattac ttaaaagatt
     1981 tgaaacgtta gcttaaaagc tagcgttttt tttatgtctt tttgcccctg cgggaactat
     2041 agcgttcaac cggcatggtt gcccaatttt catgttaatt tttaaatttg catgtaactg
     2101 ggcagtgttt attttttctt gcccattttg ctaactttta agtttgcata taaatgggca
     2161 gtgtctaaaa aattccccac tggtcatgaa caaaaaaatg agctaaacaa tttaactttt
     2221 taagttatat aaattttagg gagttccaat caaattagaa aaatatataa aagagctata
     2281 tcgtttttct ggcttatgtt taaaaagtac aagtgattgt atttaaaatg actttagcaa
     2341 tttaagtaat atgtcttttg aatacaagtt aaatgtcaga aagatgtacc cttattttgc
     2401 tcatatttgc gttttaagaa atgaatatga ttatacgtat ataaaactat ataaatttag
     2461 agtttgctct aaataccccc ttaaaattca aaataaaggc atttaaaaat taagaaaata
     2521 aaaaagctac tagcaaaaaa ctagtagctt aaatcttagt taaatttcta tgtagtatta
     2581 atttttatat ctacgctaca acgtcgattc tagttcgtgc agtattaatt tttatatctg
     2641 cgccacaacg ccgattcctt aaaactaaga taaaaattga cgaaggtcga ttatggtttt
     2701 ttacgcgttc tttttagaac gcataagtgc gcccttacgg gatttaacta gattataacg
     2761 acgaaaaata gacctgtaaa gcaataacag ttgggtctac tttgtctact gggcagacaa
     2821 gaaaagaaca cgcatttatg ccgagaaaat ttattgatgt tgagaagaac ccttaactaa
     2881 acttgcagac gaatgtcggc atagcgtgag ctattaagcc gaccattcga caagttttgg
     2941 gattgttaag ggttccgagg ctcaacgtca ataaagcaat tggaataaag caactttgtt
     3001 atttataatt atttgattgg aggtgtgaat atgtttttta agtgttct
//
