LOCUS       CP102660                6539 bp    DNA     circular BCT 21-AUG-2022
DEFINITION  Staphylococcus haemolyticus strain 4M plasmid unnamed2, complete
            sequence.
ACCESSION   CP102660
VERSION     CP102660.1
DBLINK      BioProject: PRJNA865942
            BioSample: SAMN30148943
KEYWORDS    .
SOURCE      Staphylococcus haemolyticus
  ORGANISM  Staphylococcus haemolyticus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6539)
  AUTHORS   Brovko,F. and Sokolov,S.
  TITLE     Identification and pathogenic (toxigenic) potential of
            Staphylococcus haemolyticus and Bacillus paranthracis consortium
            isolated from bovine mastitis in Russia
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6539)
  AUTHORS   Brovko,F. and Sokolov,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-AUG-2022) Laboratory of Immunochemistry, Shemyakin and
            Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy
            of Sciences, Miklukho-Maklaya, 16/10, Moscow 117997, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2020
            Assembly Method                   :: Flye v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 08/15/2022 09:54:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 2,597
            CDSs (total)                      :: 2,508
            Genes (coding)                    :: 2,463
            CDSs (with protein)               :: 2,463
            Genes (RNA)                       :: 89
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 45
            CDSs (without protein)            :: 45
            Pseudo Genes (ambiguous residues) :: 0 of 45
            Pseudo Genes (frameshifted)       :: 20 of 45
            Pseudo Genes (incomplete)         :: 24 of 45
            Pseudo Genes (internal stop)      :: 13 of 45
            Pseudo Genes (multiple problems)  :: 10 of 45
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from staphylococcus haemolyticus.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6539
                     /organism="Staphylococcus haemolyticus"
                     /mol_type="genomic DNA"
                     /strain="4M"
                     /isolation_source="milk"
                     /host="bovine"
                     /db_xref="taxon:1283"
                     /plasmid="unnamed2"
                     /geo_loc_name="Russia: Moscow region"
                     /collection_date="2020"
     gene            511..1773
                     /locus_tag="NRZ53_12945"
     CDS             511..1773
                     /locus_tag="NRZ53_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]; GO_process:
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="UVD90848.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYD
                     LKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFE
                     ESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQDKF
                     PEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYSDKVK
                     SDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDND
                     RLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSK
                     ISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNE
                     FELTHNREVKKERSRDQGMSL"
                     /name="plasmid recombination protein"
     gene            2017..3000
                     /locus_tag="NRZ53_12950"
     CDS             2017..3000
                     /locus_tag="NRZ53_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]; GO_process:
                     GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="UVD90849.1"
                     /translation="MCNNNSINSDEKSSVLIDKTKSGKIRPWREKKMANVDYFELLHIL
                     EFKKAERVKDCAEVLEYKVSYETGEKTLYRVWFCKSRLCPMCNWRRAMKHGIQSQKVVA
                     EVIKQKPTVRWLFLTLTVRNVYDGTELNKSLSDMSKGFNRMMKYKKINKNLVGFMRATE
                     VTVNNIDNSYNQHMHVLVCVESTYFYNTENYVNQKQWIQFWKRAMKLDYDPNVKVQMIR
                     PKNKHKSDIKSAIDETAKYPVKDIDFTTNDEEKNLKRLSDLEEGLHRKRLISYGGLLKE
                     IHKKLNLDDAEDGDLIHTDDEEKADEDGFSIIAMWNWERQNYFIKE"
                     /name="protein rep"
     gene            3079..3471
                     /locus_tag="NRZ53_12955"
     CDS             3079..3471
                     /locus_tag="NRZ53_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="UVD90850.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCYT
                     LLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRACH
                     VYEKLGGKNDYKDEIVYVYDYEKGDK"
                     /name="GNAT family N-acetyltransferase"
     gene            3472..4911
                     /gene="aph(2'')-Ia"
                     /locus_tag="NRZ53_12960"
     CDS             3472..4911
                     /gene="aph(2'')-Ia"
                     /locus_tag="NRZ53_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="UVD90846.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLES
                     LKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFIGE
                     PNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLPEHE
                     LHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNE
                     YIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTF
                     LTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIY
                     NDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGI
                     IDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIK
                     NIKQEFIENGRKEIYKRTYKD"
                     /name="aph(2'')-Ia"
     gene            5110..6486
                     /gene="tet(L)"
                     /locus_tag="NRZ53_12965"
     ARG             5111..6483
                     /name="tet(L)"
                     /gene="tet(L)"
                     /gene_name="tetracycline efflux MFS transporter Tet(L)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             3473..4908
                     /name="aac(6')-Ie/aph(2'')-Ia"
                     /gene="aac(6')-Ie/aph(2'')-Ia"
                     /gene_name="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             3473..4911
                     /name="AAC(6')-Ie-APH(2'')-Ia bifunctional protein"
                     /gene="AAC(6')-Ie-APH(2'')-Ia bifunctional protein"
                     /gene_name="aminoglycoside bifunctional resistance protein"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          2018..2999
                     /name="rep_cluster_2104"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.727"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          512..1770
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.19"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acaggaattc aaatcttttt gttccattaa agggcgcgat tgctgaataa aagatacgag
       61 agacctctct tgtatctttt ttattttgag tggttttgtc cgttacacta gaaaaccgaa
      121 agacaataaa aattttattc ttgctgagtc tggctttcgg taagctagac aaaacggaca
      181 aaataaaaat tggcaagggt ttaaaggtgg agattttttg agtgatcttc tcaaaaaata
      241 ctacctgtcc cttgctgatt tttaaacgag cacgagagca aaacccccct ttgctgaggt
      301 ggcagagggc aggttttttt gtttcttttt tctcgtaaaa aaaagaaagg tcttaaaggt
      361 tttatggttt tggtcggcac tgccgacagc ctcgcagagc acacacttta tgaatataaa
      421 gtatagtgtg ttatacttta cttggaagtg gttgccggaa agagcgaaaa tgcctcacat
      481 ttgtgccacc taaaaaggag cgatttacat atgagttatg cagtttgtag aatgcaaaaa
      541 gtgaaatcag ctggactaaa aggcatgcaa tttcataatc aaagagagcg aaaaagtaga
      601 acgaatgatg atattgacca tgagcgaaca cgtgaaaatt atgatttgaa aaatgataaa
      661 aatattgatt acaacgaacg tgtcaaagaa attattgaat cacaaaaaac aggtacaaga
      721 aaaacgagga aagatgctgt tcttgtaaat gagttgctag taacatctga ccgagatttt
      781 tttgagcaac tggatccagg agaacaaaaa cgattttttg aggaaagtta taaattattt
      841 tccgaacgat atggcaagca aaatattgct tatgcaacag ttcataatga tgagcaaacc
      901 cctcacatgc atttaggtgt tgtgcctatg cgtgatggaa aactgcaagg aaaaaatgtg
      961 tttaatcgtc aagaactgtt atggctacaa gataaattcc ccgagcatat gaaaaaacag
     1021 ggttttgagt tgaagcgtgg tgaacgtggc tctgaccgta aacatattga gacagctaaa
     1081 tttaaaaaac aaactttgga aaaagagatt gattttctag aaaaaaattt agcagttaaa
     1141 aaagatgaat ggactgctta tagcgataaa gttaaatcag atttagaagt accagcgaaa
     1201 cgacacatga aaagtgttga agtgccaacg ggtgaaaagt ccatgtttgg tttgggaaaa
     1261 gaaataatga aaacagaaaa gaaaccaacc aaaaatgttg ttatatcgga gcgtgattat
     1321 aaaaacttag tgactgctgc gagagataac gataggttaa aacagcatgt tagaaatctc
     1381 atgagtactg atatggcgag agaatataaa aaattaagta aagaacatgg gcaagttaaa
     1441 gaaaaatata gtggtcttgt agagcgattt aatgaaaatg taaatgatta taatgagttg
     1501 cttgaagaaa acaagtcttt aaagtctaaa ataagcgatt taaagcgtga tgtgagttta
     1561 atctatgaaa gcactaagga attccttaag gaacgtacag acggcttaaa agcctttaaa
     1621 aacgttttta aggggtttgt agacaaggta aaggataaaa cagcacaatt ccaagaaaaa
     1681 cacgatttag aacctaaaaa gaacgaattt gaactaactc ataaccgaga ggtaaaaaaa
     1741 gaacgaagtc gagatcaggg aatgagttta taaaataaaa aaagcacctg aaaaggtgtc
     1801 tttttttgat ggttttgaac ttgttctttc ttatcttgat acatatagaa acaacaagtt
     1861 tttattttta cagtttacat tacgcttaaa gtattggtgt gattggtttt ataaggttaa
     1921 tgaaccttta aaaatccttg cacagaaaaa taccctatgt tattgtttaa gttaccacac
     1981 aaaaacaaat ttacataggg ggtttctgta ttcattatgt gcaataataa tagcattaat
     2041 tcagatgaaa aatcaagtgt actaatcgac aaaacaaaaa gtggaaaaat aagaccttgg
     2101 cgagaaaaga aaatggcaaa tgtagattat tttgaactct tgcatattct tgaatttaaa
     2161 aaggctgaac gtgtaaaaga ttgtgcagag gtacttgaat ataaagtgag ttatgaaaca
     2221 ggagaaaaaa cgttatatcg tgtgtggttt tgtaaatctc gattatgtcc aatgtgcaac
     2281 tggaggagag caatgaagca tggtattcag tcacaaaagg ttgttgcaga agttattaaa
     2341 caaaaaccaa cagttcgttg gcttttcctt acgttgaccg ttagaaatgt ttatgatggt
     2401 actgaattaa ataaaagttt atcggatatg tcaaaaggtt ttaatcgtat gatgaaatat
     2461 aagaaaatta ataaaaactt agttggtttt atgcgagcaa cagaagtaac tgtaaataat
     2521 atagataatt cttataacca acacatgcat gtgttggttt gtgtagaaag cacttatttt
     2581 tataacactg aaaactatgt gaatcaaaag caatggattc aattttggaa aagagctatg
     2641 aaattagatt atgatccaaa cgttaaagtc caaatgattc gaccaaaaaa taaacataaa
     2701 tctgatataa aatcagccat tgatgaaact gcaaaatatc cagtaaagga tattgatttt
     2761 acaacaaatg acgaagaaaa gaatttgaag cgtttatctg atttggagga aggtttacac
     2821 cgtaaaaggt taatctctta tggtggtttg ctaaaagaaa tacataaaaa attaaacctt
     2881 gatgatgccg aagatgggga tttgattcat acagatgatg aagaaaaagc agatgaagat
     2941 ggattttcaa ttattgcaat gtggaactgg gaaagacaaa attattttat aaaagagtag
     3001 ttcaacaaac gggccatatt gttgtataag tgatgaaata ctgaatttaa aacttagttt
     3061 atatgtggta aaaagtttat ggaagaagtt ttaattgatg ataatatggt ttttgatatt
     3121 gataatttaa aaggatttct taatgatacc agttcatttg ggtttatagc taaagaaaat
     3181 aataaaatta taggatttgc atattgctat acacttttaa gacctgatgg aaaaacaatg
     3241 ttttatttac actcaatagg aatgttacct aactatcaag acaaaggtta tggttcaaaa
     3301 ttattatctt ttattaagga atattctaaa gagattggtt gttctgaaat gtttttaata
     3361 actgataaag gtaatcctag agcttgccat gtatatgaaa aattaggtgg taaaaatgat
     3421 tataaagatg aaatagtata tgtatatgat tatgaaaaag gtgataaata aatgaatata
     3481 gttgaaaatg aaatatgtat aagaacttta atagatgatg attttccttt gatgttaaaa
     3541 tggttaactg atgaaagagt attagaattt tatggtggta gagataaaaa atatacatta
     3601 gaatcattaa aaaaacatta tacagagcct tgggaagatg aagtttttag agtaattatt
     3661 gaatataaca atgttcctat tggatatgga caaatatata aaatgtatga tgagttatat
     3721 actgattatc attatccaaa aactgatgag atagtctatg gtatggatca atttatagga
     3781 gagccaaatt attggagtaa aggaattggt acaagatata ttaaattgat ttttgaattt
     3841 ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa taatccaaga
     3901 gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc agaacatgaa
     3961 ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga tgataatgcc
     4021 acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt caaagtagat
     4081 agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa taatgaatac
     4141 atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga aaaagcaata
     4201 tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat tgaatattcg
     4261 tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac ttttttaaca
     4321 ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg agatattgcc
     4381 agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg tactattgat
     4441 aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta taatgattta
     4501 actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc aacaacagtt
     4561 tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct attgttagat
     4621 ggcaataata gattaactgg aataattgat tttggagatt ctggaattat agatgaatat
     4681 tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa ttttggagaa
     4741 gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata tcaagatata
     4801 gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat taaacaggaa
     4861 tttatcgaaa atggtagaaa agaaatttat aaaaggactt ataaagattg attatataat
     4921 atatgaaaag ctattataaa agacattagt attaaatagt ttaaaaaaat gaaaaataat
     4981 aaaggtttta atcaagttta ggaggaatta attatgaagt gtaatgaatg taacagggtt
     5041 caattaaaag agggaagcgt atcattaacc ctataaacta cgtctgccct cattattgga
     5101 gggtgaaatg tgaatacatc ctattcacaa tcgaatttac gacacaacca aattttaatt
     5161 tggctttgca ttttatcttt ttttagcgta ttaaatgaaa tggttttgaa cgtctcatta
     5221 cctgatattg caaatgattt taataaacca cctgcgagta caaactgggt gaacacagcc
     5281 tttatgttaa ccttttccat tggaacagct gtatatggaa agctatctga tcaattaggc
     5341 atcaaaaggt tactcctatt tggaattata ataaattgtt tcgggtcggt aattgggttt
     5401 gttggccatt ctttcttttc cttacttatt atggctcgtt ttattcaagg ggctggtgca
     5461 gctgcatttc cagcactcgt aatggttgta gttgcgcgct atattccaaa ggaaaatagg
     5521 ggtaaagcat ttggtcttat tggatcgata gtagccatgg gagaaggagt cggtccagcg
     5581 attggtggaa tgatagccca ttatattcat tggtcctatc ttctactcat tcctatgata
     5641 acaattatca ctgttccgtt tcttatgaaa ttattaaaga aagaagtaag gataaaaggt
     5701 cattttgata tcaaaggaat tatactaatg tctgtaggca ttgtattttt tatgttgttt
     5761 acaacatcat atagcatttc ttttcttatc gttagcgtgc tgtcattcct gatatttgta
     5821 aaacatatca ggaaagtaac agatcctttt gttgatcccg gattagggaa aaatatacct
     5881 tttatgattg gagttctttg tgggggaatt atatttggaa cagtagcagg gtttgtctct
     5941 atggttcctt atatgatgaa agatgttcac cagctaagta ctgccgaaat cggaagtgta
     6001 attattttcc ctggaacaat gagtgtcatt attttcggct acattggtgg gatacttgtt
     6061 gatagaagag gtcctttata cgtgttaaac atcggagtta catttctttc tgttagcttt
     6121 ttaactgctt cctttctttt agaaacaaca tcatggttca tgacaattat aatcgtattt
     6181 gttttaggtg ggctttcgtt caccaaaaca gttatatcaa caattgtttc aagtagcttg
     6241 aaacagcagg aagctggtgc tggaatgagt ttgcttaact ttaccagctt tttatcagag
     6301 ggaacaggta ttgcaattgt aggtggttta ttatccatac ccttacttga tcaaaggttg
     6361 ttacctatgg aagttgatca gtcaacttat ctgtatagta atttgttatt acttttttca
     6421 ggaatcattg tcattagttg gctggttacc ttgaatgtat ataaacattc tcaaagggat
     6481 ttctaaatcg ttaagggatc aactttggga gagagttcaa aattgatcct ttttttata
//
