LOCUS       CP101805                5268 bp    DNA     circular BCT 04-AUG-2022
DEFINITION  Escherichia coli strain EC802A1 plasmid p802A1-5K, complete
            sequence.
ACCESSION   CP101805
VERSION     CP101805.1
DBLINK      BioProject: PRJNA827955
            BioSample: SAMN27625735
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5268)
  AUTHORS   TANG,B., Chang,J., Yang,H., Wu,J. and Li,J.
  TITLE     Complete genome sequences of Escherichia coli isolates in Zhejiang
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5268)
  AUTHORS   Tang,B., Chang,J., Wu,J., Yang,H., Lin,J., Guan,C., Jiang,H.,
            Chen,H. and Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
REFERENCE   3  (bases 1 to 5268)
  AUTHORS   TANG,B., Chang,J., Yang,H., Wu,J. and Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JUL-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. JUL-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 07/26/2022 02:44:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,401
            CDSs (total)                      :: 5,274
            Genes (coding)                    :: 4,948
            CDSs (with protein)               :: 4,948
            Genes (RNA)                       :: 127
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 107 of 326
            Pseudo Genes (incomplete)         :: 216 of 326
            Pseudo Genes (internal stop)      :: 56 of 326
            Pseudo Genes (multiple problems)  :: 47 of 326
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC802A1"
                     /isolation_source="anal swab"
                     /db_xref="taxon:562"
                     /plasmid="p802A1-5K"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2021-08"
     gene            complement(join(4421..5268,1..265))
                     /locus_tag="MZH05_26975"
     CDS             complement(join(4421..5268,1..265))
                     /locus_tag="MZH05_26975"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="UUJ96387.1"
                     /translation="MAKGLELAGFQHVGFVELNKHACDSLRLNFDEEKVFQGDIKNYDL
                     SSIDKIDIVAGGPPCQPFSLGGKHKAHDDSRDMFPFAIKAIEVLQPKAFIFENVKGLLR
                     KSFADYFEYIILRLTYPCLSLSNVSDWRIHLDALRKARKDKHCGCTYDVSFKLINAADY
                     GVPQIRERVIIVGVRSDLNKKWSFPSVTHTQEKLLWEQHVTGDYWRKHNIDAVIDKGIS
                     ERLMKRFGIFPPSGKSWVTVRDALKNVPQPYESHGIGDHIFKDGARVYPGHTGSYIDLP
                     SKTLKAGAHGVPGGENMIRYEDGTVRYFTVYEGKLLQTFPREFLIAGAWGEAMRQIGNA
                     VPVLLAQKIGQQLYNLLNEEVEPLLCSNFN"
                     /name="DNA cytosine methyltransferase"
     gene            complement(301..945)
                     /locus_tag="MZH05_26980"
     CDS             complement(301..945)
                     /locus_tag="MZH05_26980"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eco29kI family restriction endonuclease"
                     /protein_id="UUJ96388.1"
                     /translation="MHNKKFDRSEHVYRNDSFLELIKDAVRFFSGTPVHSLPPPERFQG
                     AGVYALYYTGHYSLYDEYSRINRLAYNLPIYVGKAVPAGWRQSRISDHETRAGSELSNR
                     IREHSRNIAKTSNLNLCDFSCRFVIFEATGSDMISTVEAALIKIYKPLWNTVVDGFGNH
                     TPGAGRFAQAKSDWDVIHPGREWAEKCTGVHSEPYFIEERIKQYFSKSNFT"
                     /name="Eco29kI family restriction endonuclease"
     gene            complement(1345..1578)
                     /locus_tag="MZH05_26985"
     CDS             complement(1345..1578)
                     /locus_tag="MZH05_26985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UUJ96389.1"
                     /translation="MTELEIHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1585..2103)
                     /locus_tag="MZH05_26990"
     CDS             complement(1585..2103)
                     /locus_tag="MZH05_26990"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="UUJ96390.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQTNLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(2141..2572)
                     /locus_tag="MZH05_26995"
                     /pseudo=""
     CDS             complement(2141..2572)
                     /locus_tag="MZH05_26995"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2781..3104)
                     /locus_tag="MZH05_27000"
     CDS             complement(2781..3104)
                     /locus_tag="MZH05_27000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UUJ96385.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3168..3359
                     /locus_tag="MZH05_27005"
     CDS             3168..3359
                     /locus_tag="MZH05_27005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UUJ96386.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(3445..3530)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="8.58e-38"
     Typing          3552..4099
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.863"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1242..2791)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.565"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atacttcaat tgcttttatt gcaaatggga acatatccct agagtcatca tgagctttat
       61 gttttccgcc caatgaaaat ggttggcagg gaggcccgcc agcaacaata tctattttat
      121 ctatagatga aaggtcataa tttttaatat ctccctgaaa taccttctcc tcatcaaaat
      181 taagtcgcaa agaatcacat gcatgcttgt ttaactcaac aaatcctaca tgctgaaatc
      241 ctgccaactc tagaccttta gccaaaccgc cggctcctga aaaaatctct aatgatctca
      301 ttatgtaaaa ttactcttag aaaaatactg tttaattctc tcctcaataa agtatggctc
      361 agaatgaaca cctgtacatt tctcagccca ctccctgcca ggatgtatta catcccaatc
      421 agatttagct tgcgcaaatc gcccggcacc aggggtatga tttccaaagc catcaacaac
      481 tgtattccac aaaggcttat aaattttaat aagagcggcc tcaactgtac taatcatatc
      541 agaccccgta gcctcaaata taacaaagcg acatgaaaaa tcacacaaat ttagattcga
      601 tgtttttgct atatttcggc tatgttctct aattctatta gatagttcag aaccagccct
      661 agtttcatga tcactaattc tagactgacg ccaaccagca ggaacagcct tgccaacata
      721 aataggaagg ttatatgcca acctatttat acgagagtat tcatcatata atgaataatg
      781 ccctgtatag taaagagcat acaccccagc accttgaaac cgttccggtg gtggtaatga
      841 atgaacaggc gtaccactaa aaaaacgaac ggcatctttt attaactcta ggaaagagtc
      901 atttctataa acatgctcag atctatcaaa tttcttattg tgcattatgt tttccgaaag
      961 gtttttatat taacactcct actcaacatc acctatgata cgatcataaa gctgataccc
     1021 aaaaccatac gcatttacca taacgtcgca ttacgcgcac cgctaaaacg cgttcagcgc
     1081 gatcacggca gcagacaggt aaaaatggca acaaaccacc cgaaaaactg ccgcgatcgc
     1141 gcctgataaa ttttaaccgc atgaatacct atgcaaccag atggtacagg ccacattaac
     1201 ccctcttaat ccactgaagc tgccattttt catggtttca ccatcccagc gaaggaccat
     1261 ccagcgtgcg ttcctgtatt tccggctgac gctcccgttc cagggataac gcatgttcgc
     1321 gctcctgtat cagccgttcc tctcttatct ccagttctcg ctgtataact ggctcaagcg
     1381 ttctgtctgc tcgctcaagt gctgcacctg ctgactcaac tgcatgaccc gctcgttcag
     1441 catcgcgttg tcccgttgcg taagcgaaaa catcttctgc aattccacga aggcgctctc
     1501 ccattcgctc agccgctgca tatagtcctg ttgcagctgc tctaaggcgt tcagcaaatg
     1561 tatttccagc tctgtcactc tgtgtcactc cttcagatgt acccactctt tcccctgaaa
     1621 gggaatcacc tccgctgatt tcccgtacgg aaggacaagg aatttcctgt tcccgtcctg
     1681 cacaaactcc acgccccatg tcttcgcgtt cagtttctgc aatgtctctt cctgcttcct
     1741 gatttcttcc aggttcgtct gtatccgtcc gccaagatac cagagcgtcc cgccactcgc
     1801 ggtaaacagg agaaagacta tccccagtaa catcatgccc gtattccctg ccagctttaa
     1861 cacgtccttc ctgtgctgca tcatcgcctc tttcacccct tcccggtgtt tttccagcga
     1921 tttctctgtc gaggctgtga acagggctat agcgtctctg attttcgtct cgtttgaggt
     1981 cacagcctcg ctgacagact cgccgagctt cctgaactcg tcgttcagca ttttctctgt
     2041 agattcggct ctctctttca gctttttctc gaactccgcg cccgtctgta aaagattgct
     2101 cataaaatgc tcctttcagc ctgatattct tcccgccgtt cgggtctgca atgctgatac
     2161 tgcttcgcgt caccctgacc acttcaagcc ccgcctctgt gagcgcctga atcacatcct
     2221 gacggccttt tatctctccg gcatggtaaa gtgcgtctat accgcgtgta acgctctcag
     2281 caagtgcctg tttcgtttcc ggcaggttat caggaagggt cagcgtccgg cggttctccg
     2341 gggcgttggg gtcatgcagt ccgtaatggt gattcaccag cgtctgccag gcatcaattc
     2401 tcggcctgtc tgcgcggtcg tagtacggct ggagacgttt tccggtctgt agctccatgt
     2461 tcggaatgac aaaattcagc tcaagccgtc ccttgtcctg gtgttccacc cacaggatgc
     2521 tgtactgatt tttttcgaga ccgggcatca gtacacgctc aaagctcgcc atcacttttt
     2581 cacgtcctcc cggtggcagc tccttctccg cgaacgacag cacaccggac gtgtatttct
     2641 tcgcaaatgg cgtggcatcg atgagttccc gtacttcttc cggattaccc tgaagcaccg
     2701 ttgccccttc ccggttacgc tcccttccca gcaggtaatc aaccggacca ctgccaccac
     2761 cttttcccct ggcatgaaac ttaactatca tcccgcgtcc cctgttccct gacggccaga
     2821 cgcagccggc gcagctcatc cccgatggcc atcagtgcgg ccaccacctg aacccggtca
     2881 ccggaagacc actgcccgct gttcacctta cgggctgtct gattcaggtt atttccgatg
     2941 gcggccagct gacgcagtaa cggcggtgcc agtgtcggca gttttccgga acgggcaacc
     3001 ggctcaccca ggcagacccg ccgcatccat accgccagtt gtttaccctc acagcgttca
     3061 agtaaccggg catgttcatc atcagtaacc cgtattgtga gcatcctctc gcgtttcatc
     3121 ggtatcatta ccccatgaac agaaatcccc cttacacgga ggcatcagtg actaaacagg
     3181 aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca gacattaacg ctgctggaga
     3241 agctcaacga actggacgca gatgaacagg ccgatatttg tgaatcgctt cacgaccacg
     3301 ccgatgagct ttaccgcagc tgcctcgcac gtttcgggga tgacggtgaa aacctctgac
     3361 acatgcagct cccggagacg gtcacagctt gtctgtgagc gaatgccggg agcagacaag
     3421 cccgtcaggg cgcgtcagca ggttttagcg ggtgtcgggg cgcagccctg acccagtcac
     3481 gtagcgatag cggagtgtat actggcttaa ccatgcggca tcagtgcaga taataagaaa
     3541 agagcaccat atacggtgtg aaataccgca cagatgcgta aggagaaaat gccgcatcag
     3601 gcgctcttcc gcttcctcgc tcactgactc gctacgctcg gtcgttcggc tgcggcgagc
     3661 ggtatcagct cactcaaaga cggtaatgcg gttatccaca gaatcagggg ataacgccaa
     3721 aagaaacatg tgagcaaaaa acaagaaccc ggaaaaggcc acgccgctgg cgtttttcca
     3781 taggctccgc ccccctgacg agcatcacaa aatcgacgct caagtcagag gtggcgaaac
     3841 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     3901 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     3961 ctttctcata gctcacgctg ttggtatctc agttcggtgt aggtcgttcg ctccaagctg
     4021 ggctgtgtgc acgaaccccc cgttcagccc gactgctgcg ccttgcccgg taactatcgt
     4081 cttgagtcca acccggtaag acacgcctta acgccactgg cagcagccat tggtaactgg
     4141 acaaggtgga ttgagatatt aagagttctt gaagtggtgg cctaactgag gctacactag
     4201 aaggacagta tttggtatct gcgctccacc aagccagtta ccttcgggaa aaagagttgg
     4261 tagctcttga ttccggcaaa cgaaccaccg ttggtagcgg tggttttttt gtttacaggc
     4321 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt ttttgcaaat
     4381 tgcctctatg gctgtatcac caccatagag acacaaactt ttaattgaag ttagagcaca
     4441 ataaaggctc aacctcttca ttcaataagt tatacaattg ctgtcctatc ttctgagcta
     4501 gcaacacagg aactgcatta ccaatctgcc tcatagcctc cccccaagct cctgcaatca
     4561 gaaactctcg aggaaaagtc tgaagaagct tcccttcata aaccgtaaaa taacgaacag
     4621 tcccatcctc atacctaatc atattttcac ctccaggtac gccatgagcg ccagccttaa
     4681 gtgtttttga aggcaaatcg atataacttc cagtatgacc aggataaact cgagcgccat
     4741 ctttaaagat atgatctcca ataccatgtg actcatatgg ttgaggaaca ttctttaatg
     4801 cgtccctaac agtaacccag cttttccccg atggggggaa aataccaaaa cgtttcatta
     4861 aacgctcaga aataccttta tctataaccg catcaatatt atgctttcgc caatagtcac
     4921 cagtaacatg ctgttcccac agtaattttt cttgagtatg cgttacgctt ggaaaagacc
     4981 attttttatt caaatcactt cgaacaccaa caattatgac ccgttcccta atttgtggaa
     5041 ctccataatc agctgcattg atcaatttaa aactgacatc atatgtacac ccacagtgtt
     5101 tatccttgcg agcttttctt aatgcatcta gatgtattct ccaatcactt acattactta
     5161 acgataaaca aggataagtc aaacgaagaa ttatgtattc aaaataatcc gcaaatgatt
     5221 ttctaagcaa ccctttaaca ttttcaaaaa taaaagcttt tggttgca
//
