LOCUS       CP101804                6896 bp    DNA     circular BCT 04-AUG-2022
DEFINITION  Escherichia coli strain EC802A1 plasmid p802A1-6K, complete
            sequence.
ACCESSION   CP101804
VERSION     CP101804.1
DBLINK      BioProject: PRJNA827955
            BioSample: SAMN27625735
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6896)
  AUTHORS   TANG,B., Chang,J., Yang,H., Wu,J. and Li,J.
  TITLE     Complete genome sequences of Escherichia coli isolates in Zhejiang
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6896)
  AUTHORS   Tang,B., Chang,J., Wu,J., Yang,H., Lin,J., Guan,C., Jiang,H.,
            Chen,H. and Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
REFERENCE   3  (bases 1 to 6896)
  AUTHORS   TANG,B., Chang,J., Yang,H., Wu,J. and Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JUL-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. JUL-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 07/26/2022 02:44:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,401
            CDSs (total)                      :: 5,274
            Genes (coding)                    :: 4,948
            CDSs (with protein)               :: 4,948
            Genes (RNA)                       :: 127
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 326
            CDSs (without protein)            :: 326
            Pseudo Genes (ambiguous residues) :: 0 of 326
            Pseudo Genes (frameshifted)       :: 107 of 326
            Pseudo Genes (incomplete)         :: 216 of 326
            Pseudo Genes (internal stop)      :: 56 of 326
            Pseudo Genes (multiple problems)  :: 47 of 326
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC802A1"
                     /isolation_source="anal swab"
                     /db_xref="taxon:562"
                     /plasmid="p802A1-6K"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2021-08"
     gene            complement(join(6139..6896,1..808))
                     /locus_tag="MZH05_26925"
     CDS             complement(join(6139..6896,1..808))
                     /locus_tag="MZH05_26925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0016021 - integral component of membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="UUJ96378.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGGGGGTGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNEALRH
                     NSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEKEQ
                     AETERQLKLAEDEEKRLAALSEEARAVEVAQKNLAAAQSELAKVDEEINTLNTRLSSGI
                     HARDAETNTLSGKRNELDQASAKYKELDERVKLLSPRANDPLQSRPFFEATRLRARAGD
                     EMEEKQKQVTASETRLNQISSEINGIQKAISQANNKRSTAVSRIHDAEDNLKIAQTNLL
                     NSQIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAFEKYKDVL
                     NKKFSKADRDAIFNALESVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTGD
                     WKPLFLTLEKKAVDAGVSYVVVLLFSMLAGTTLGIWGIAIVTGILCAFIDKNKLNTINE
                     VLGI"
                     /name="colicin-like pore-forming protein"
     gene            1067..1504
                     /locus_tag="MZH05_26930"
     CDS             1067..1504
                     /locus_tag="MZH05_26930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="UUJ96379.1"
                     /translation="MGHCHFMAWHTLQDALKMTGRSRSQFYRDMRAGLVSYRTSQDRRR
                     EFETSELIRAYGELKQDETPERHSAGQAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDTERRRQEAEREQLQNEIAQLRQALEQEKKRGFWSRLFGR"
                     /name="entry exclusion protein 1"
     gene            1585..2004
                     /locus_tag="MZH05_26935"
     CDS             1585..2004
                     /locus_tag="MZH05_26935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="UUJ96380.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVELHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLAETISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(2565..2798)
                     /locus_tag="MZH05_26940"
     CDS             complement(2565..2798)
                     /locus_tag="MZH05_26940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UUJ96381.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2805..3335)
                     /locus_tag="MZH05_26945"
     CDS             complement(2805..3335)
                     /locus_tag="MZH05_26945"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="UUJ96382.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSG
                     GTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETNWTM
                     ENGKQNALRLVQE"
                     /name="MbeB family mobilization protein"
     gene            complement(3373..3804)
                     /locus_tag="MZH05_26950"
                     /pseudo=""
     CDS             complement(3373..3804)
                     /locus_tag="MZH05_26950"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4013..4336)
                     /locus_tag="MZH05_26955"
     CDS             complement(4013..4336)
                     /locus_tag="MZH05_26955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UUJ96383.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVVALMAIERELRSLRLVVREQGAR
                     DDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4401..4592
                     /locus_tag="MZH05_26960"
     CDS             4401..4592
                     /locus_tag="MZH05_26960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UUJ96376.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5616..5753)
                     /locus_tag="MZH05_26965"
     CDS             complement(5616..5753)
                     /locus_tag="MZH05_26965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0019867 - outer membrane [Evidence IEA]; GO_process:
                     GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="UUJ96377.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTVAPSSSSKLTGISVQ
                     "
                     /name="colicin release lysis protein"
     gene            5915..6142
                     /locus_tag="MZH05_26970"
     CDS             5915..6142
                     /locus_tag="MZH05_26970"
                     /inference="COORDINATES: protein motif:HMM:NF015489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="UUJ96384.1"
                     /translation="MLYAIVISTILCPYSKYAIEHIFFKFIKKDFFRKRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     Typing          complement(4679..4765)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.864"
                     /coverage="100.0"
                     /evalue="1.13e-36"
     Typing          4776..5333
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.118"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2462..4023)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.697"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4020..4369)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.162"
                     /coverage="99.0"
                     /evalue="1.08e-64"
ORIGIN
        1 aaaaaggacg actctgaagc ggatcattcg ccctaggaga taacagttta accctttcat
       61 ccagttcttt atatttagca gatgcctgat ccaactcatt tcgttttccg gacagcgtat
      121 tcgtttctgc atcacgggca tgaataccgg agcttaaacg ggtattgagc gtattaatct
      181 cttcatccac tttcgccagc tcagattgtg cagcagcaag atttttttgt gccacctcca
      241 cagcccgggc ctcttcactc aatgctgcca ggcgtttctc ttcatcctca gccagtttca
      301 attggcgttc tgtttcagcc tgctccttct ctatctcttt acgtcgttgt tctgcttcct
      361 gaaaagcctt ttccgcagct tccgcttctt tacgagcttt ttcttctgct tttgcaaggc
      421 gcaaccgctc tgcttctgcc tgcatcgctg cattattggc atgagccagg tcaataacct
      481 ccggatgagt ggaattatgg cgaagcgcct cattcacaat atccttcaga tgttgagtca
      541 gcgcatcccg gtttgctttt gctttagcct gtgcttctgc ggcagctttt gctcgggcag
      601 cctgttctgc ctgcgttttc ttcaattgag cagtagacca tttagctgtg gcatgaatgg
      661 ctgcagaact ttcacttttg ctacctccag ttccaccacc accgccagag ccactcccgt
      721 ctggattacc attcaaaaga gtaatgatta cctgcccctt atcatcataa ggaacaccat
      781 ctttatagta cgctacagct gtttccatta taaaatcctc tttgacatta aaaacaaata
      841 gttaaaagca atatactgta tatataacca ctggttttat atacagctta aaattacgcc
      901 gctgcatttt ccctgtcaac cctgtggatt ttcatttttg tgaaaacgat caaaaaacag
      961 ccacccacac cacgtgctat ttccacccga tgccacaaaa accagcacaa acattaccgt
     1021 tctccggcct cagtatgttt tactgaaacc atgatatgag acaccaatgg gacactgtca
     1081 cttcatggca tggcacacac tccaggacgc actgaaaatg acaggcagat cgcgttcaca
     1141 gttttaccgt gatatgcgcg caggccttgt cagttaccgt accagccagg acagacgacg
     1201 ggagtttgaa accagtgaac tgatccgggc atacggcgaa ttaaagcagg atgagacacc
     1261 agaaaggcac agtgcgggac aggcagaaaa tccacatgat cagcagacag aacgcattct
     1321 ccgggagctg aatgagctga aacaatgcct gacgctgatg cttgaggata aacaagccca
     1381 ggatacggag cgccggcgcc aggaagcaga acgggaacag ctacagaatg agatagccca
     1441 gctcaggcag gcgctggagc aggaaaagaa acggggattc tggtcccggt tgtttggtcg
     1501 ctgaacgctg tcatggactg atgataaaat agtcttctga taataactca ccgaaaataa
     1561 atactttaag gtagggagac tctcatgaga cgtaccggaa acaaactttg tcttatcgcc
     1621 atgataacag caacagtagc tctcacagcc tgtaccccaa agggcagcgt ggaactacat
     1681 acccggcatt acgtatatgc ttctgatgac ggttttgatc ccaacttttc cacccaaaaa
     1741 gccgacacaa cacgaatgat ggtgcctttt ttccggcagt tctgggatat gggagcaaag
     1801 gataaagcga caggaaaatc acggagtgat gtgcagcaac gcattcagca gtttcacagc
     1861 caggaatttt taaactcact ccggggcaca actcaatttg cgggtactga ttaccgcagc
     1921 aaagacctta ccccgaaaaa atccaggctg ctggctgaga cgatttctgc ggtttatctc
     1981 gatggctatg agggaagaca gtaagtggat ttaccataat cccttaattg tacgcaccgc
     2041 tgaaacgcgt tcggcgcgat cacggcagca gacaggtaaa aatggcaaca aaccacccga
     2101 aaaactgccg cgatcgcacc cggtaaattt taaccacatg catagctatg cagccatgtg
     2161 aatcacgcag gaattgccag ccagagacag ctgaaacgga tttttttcat gctttcggaa
     2221 gcgaagaaga cggggaccgg agcgggaaaa cagaagttca ggggaatgag cgcgatctgg
     2281 caatagaggc aaaacacagc aacaaaagac acaccagaat cgcgcccgta tgcgttttaa
     2341 cgcgttcctg tgttttcagc aaggtctgac tgaagcgcgt tcccggtgcc cccgaaccaa
     2401 ccattatccc aaaaaatgca ccggaaacgc gctgacaaca ttttagcctg tctctgatta
     2461 ccatcccagc gaagggccat ccagtgtccg ttcctgtatt tccagctggc gctcacgctc
     2521 cagggataac tcatgttcgc gttcctgtat cagccgttcg tcccttatct cctgttctcg
     2581 ctgtataact ggctcaagcg ttctgtctgc tcgctcaagt gctgcacctg ctgactcaac
     2641 tgcatgaccc gctcgttcag catcgcgttg tcccgttgcg taagcgaaaa cattttctgc
     2701 aattccacga aggcgctctc ccattcgttc agccgctgca tatagtcctg ttgcagttgc
     2761 tctaatgcgt tcagcaaatg tctttccagt tccgtcatgc tctgttactc ctgtaccagc
     2821 cttaaggcat tctgtttccc gttttccatc gtccagttcg tttcggcttt catcccctcg
     2881 ggcaacacca gaaaacgtcc gttgctcccc tcgtgataac gcacgcccca tgtcttcgcg
     2941 ttcagcttct ccagactctc gttctgcttc ctgatttctt ccaggttcgc ctgtatcatc
     3001 ccgccaagat accagagcgt cccgccactc acggtaaaca ggaaaatgac catccccagt
     3061 aacatcatgc ccgtattccc tgccagcttt aacacgtcct tcctgtgctg catcatcgcc
     3121 tctttcaccc cttcccggtg ttttttcagt gattcctctg tcgaagctgt gaacagggct
     3181 atagcatctt tgattttcgt ctcgtttgat gtcacagcct tgcttacaga ttcttcgagc
     3241 ttcctgaact cgtcgttcag cattttctct gtagattcgg ctctctcttt cagctttttc
     3301 tcgaactccg cgcccgtctg taaaagattg ctcatagaat gctcctttca gtctgatatt
     3361 cctcccgccg ttcgggtctg caatgctgat actgcttcgc gtcaccctga ccacttcaag
     3421 tcccgcctcc gtgagcgcct gaatcacatc ctgacgacct tttatctctc cggcatggta
     3481 aagcgcgtct atcccgcgtg taacgccctc agcaatcgcc tgtttcgttt ccggcagatt
     3541 atcagggagt gtcagcgtcc tgcggttctc cggggcgttc gggtcatgca gcccgtaatg
     3601 gtgattaacc agcgtctgcc aggcatcaat tctaggcctg tctgcccggt cgtagtacgg
     3661 ctggaggcgt tttccgctct gtagctccat gttcggaatg acaaaattca gctcaagccg
     3721 tcccttgtcc tggtgctcca cccacaggat gctgtactga tttttttcga gaccgggcat
     3781 cagtacacgc tcaaagctcg ccatcacctt ttcacgtcct cccggcggca gctccttctc
     3841 cgcgaacgac agaacacctg acgtgtattt cttcgcaaat ggcgtggcat cgatgagttc
     3901 ccggacttct tccggattac cctgaagcac cgttgcacct tcccggttac gctcccttcc
     3961 caacaggtaa tcaaccggac cactgccacc gccttttccc ctggcatgaa atttaacaat
     4021 catcccgcgc tccctgctcc ctgacgacca gccgtaagct gcgcaactct ctctcgatgg
     4081 ccatcagtgc gaccaccacc tgaacccggt caccggaaga ccactgcccg ctgtttacct
     4141 tcctcgctgt ctgattcagg ttattcccga tggcggccag ctgacgcagt aacggcggtg
     4201 ccagtgtcgg cagcttcccg gaacgggcaa ccggctcacc cagacagacc cgccgcatcc
     4261 ataccgccag ctgtttaccc tcacagcgtt ccagtaaccg tgcatgttca tcatcagtaa
     4321 cccgtatcgt gagcatcctc tcgcgtttca tcggtatcat tacccccatg aacagaaatc
     4381 ccccttacac ggaggcatca gtgaccaaac aggaaaaaac cgcccttaac atggcccgct
     4441 ttatcagaag ccagacatta acgctgctgg agaagctcaa cgaactggac gcggatgaac
     4501 aggcagacat ctgtgaatct cttcacgacc acgccgatga gctttaccgc agttgcctcg
     4561 ctcgcttcgg ggatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     4621 cttgtctgtg agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggttttt
     4681 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct
     4741 taaccatgcg gcatcagtgc ggattgtatg aaaagtgcgc catgccgggt gtgaaatgcc
     4801 gcacagatgt gtaaggagaa aatgcaactt ccgacgctct tccgcttcct cgctcactga
     4861 ctcgctacgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa agacggtaat
     4921 gcggttatcc acagaatcag gggataacac caaaagaaac atgtgagcaa aaaacaagaa
     4981 cccagagaag gccacgccgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     5041 caaaaaccga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     5101 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     5161 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgttggta
     5221 tctcggctcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     5281 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     5341 cttagcgcca ctggcagcag ccgttggtaa cttggttagc agagcgatga tggcgcaaat
     5401 ggtgcaacag agttcttgaa gtagtggccc gactacggct acactggaag gacagtattt
     5461 ggtatctgcg ctctgctgaa gccagttacc tcggttaagc agttccccaa ctgacttaac
     5521 cttcgatcaa accgcctccc caggcggttt tttcgtttac aggcagcaga ttacgcgcag
     5581 aaaaaaagga tctcaagaag atcctttgat cttttctact gaacagagat ccccgtcaat
     5641 ttagaagaag atgatggagc cacagtccct ccctgaacat cacgtatata gttagcctga
     5701 catccaacaa ggaggtttat agcgaatatt cccacaaaaa atcttttcct cataactcga
     5761 tccttataaa atgaaaagaa tatataacga ggtttaattt atgagtttaa gatactacat
     5821 aaaaaatatt ttgtttggcc tatactgcgc acttatatat atatatctta taacaaaaaa
     5881 caacgaaggg tattatttcc tagcgtcaga taagatgcta tacgcaatag tgataagcac
     5941 tattttatgc ccatattcaa aatatgctat tgaacacata ttttttaagt tcataaagaa
     6001 agattttttc agaaaaagaa aaaacctaaa taatgccccc gtggcaaaat taaacctatt
     6061 tatgctatac aatctacttt gtttggtcct agcaatccca tttggattgc taggactgtt
     6121 tatatcaata aagaataatt aaatacccaa cacctcattt atagtattaa gtttattctt
     6181 atcaataaaa gcacatagaa tgcctgtaac aatagcaatc ccccagatac ctaatgtagt
     6241 tccagcaagc atactaaaaa gtaaaacaac aacataacta actccagcat ctacagcttt
     6301 cttctctaat gtaagaaata gtggcttcca gtcacctgta tccttaattt ttaggatatc
     6361 agataccaca tcatatccaa aagaaacatg tcccgtaatc ttcaagtact tagcaaactg
     6421 atctaaatgc ttagcccagt cttcatactt aaccgactcc agtgcattga aaattgcatc
     6481 acggtctgct ttgctgaatt tcttatttaa aacatccttg tatttttcaa aagcagctag
     6541 agcttcattc acattgctga ttttcttacc tttagattta tcagcaagtt cctgtgccat
     6601 ttttgaatat ttttcaccat atttttcaga tagcgtttga taaaagctaa ctgttgcatc
     6661 cacagcatcc ttaatctgcg agttcaggag attagtctgt gctattttca aattatcttc
     6721 agcatcatga atacgtgaaa ctgctgtact tcgcttatta ttagcctgag aaatagcctt
     6781 ttggattcca tttatctcag agctaatctg gttaagacgc gtttctgatg ctgttacctg
     6841 cttttgtttt tcctccatct catcaccagc tctcgcccgt agtctggtgg cctcaa
//
