LOCUS       CP101715               15515 bp    DNA     circular BCT 16-AUG-2023
DEFINITION  Synechocystis sp. B12 plasmid unnamed6, complete sequence.
ACCESSION   CP101715
VERSION     CP101715.1
DBLINK      BioProject: PRJNA860280
            BioSample: SAMN29837452
KEYWORDS    .
SOURCE      Synechocystis sp. B12
  ORGANISM  Synechocystis sp. B12
            Bacteria; Cyanobacteriota; Cyanophyceae; Synechococcales;
            Merismopediaceae; Synechocystis.
REFERENCE   1  (bases 1 to 15515)
  AUTHORS   Pierotti,D., Mendel,D., Atum,S., Stevani,C. and Freire,R.
  TITLE     Genome sequencing of Synechocystis sp. B12
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15515)
  AUTHORS   Pierotti,D., Mendel,D., Atum,S., Stevani,C. and Freire,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUL-2022) Departamento de Quimica Fundamental,
            Instituto de Quimica - Universidade de Sao Paulo, Av. Professor
            Lineu Prestes, Sao Paulo, Sao Paulo 05508000, Brazil
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 15-JUL-2022
            Assembly Method                   :: Flye v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 128.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 07/25/2022 16:04:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,290
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 3,954
            CDSs (with protein)               :: 3,954
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1,284
            CDSs (without protein)            :: 1,284
            Pseudo Genes (ambiguous residues) :: 0 of 1,284
            Pseudo Genes (frameshifted)       :: 1,127 of 1,284
            Pseudo Genes (incomplete)         :: 159 of 1,284
            Pseudo Genes (internal stop)      :: 135 of 1,284
            Pseudo Genes (multiple problems)  :: 135 of 1,284
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from Cassius Stevani, Av. Prof.
            Lineu Prestes, 748 B. 8T room 809  05508-000 Sao Paulo, SP -
            Brasil.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..15515
                     /organism="Synechocystis sp. B12"
                     /mol_type="genomic DNA"
                     /strain="B12"
                     /isolation_source="mangrove water from the port of Santos,
                     Brazil"
                     /db_xref="taxon:2764086"
                     /plasmid="unnamed6"
                     /geo_loc_name="Brazil: port of Santos"
                     /collection_date="2020-08-19"
     gene            complement(join(15061..15515,1..40))
                     /locus_tag="NON20_26135"
     CDS             complement(join(15061..15515,1..40))
                     /locus_tag="NON20_26135"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WLT40675.1"
                     /translation="MMMGGYIQNFDSIITVGRNFVKASDRRRVDKLTEDTGNAFSPDVN
                     GACLTTKVRVMELVNIQQFFQGEHTSESLQSWFNELVKVRRDIKIGLGMSINPERDTPI
                     AVAQRLLGLLGLKMQGHQHRLNGKRIRTYTLTDDLPPERVELFTRWLERDFARVPFEEI
                     A"
                     /name="hypothetical protein"
     gene            complement(join(15491..15515,1..359))
                     /locus_tag="NON20_26140"
     CDS             complement(join(15491..15515,1..359))
                     /locus_tag="NON20_26140"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WLT40676.1"
                     /translation="MSAIQNSGFKAYRESILDKLQSEGYEIKDGGDPDDGAEVKADMKE
                     AKESSYLSHCEEVANSPLLTEKEYQEVSKARAKTPQERLGSEKDRNFPTLCHPRDFPAM
                     VADDDGGLYPKLRLHYHRGQEFC"
                     /name="hypothetical protein"
     gene            complement(304..1017)
                     /locus_tag="NON20_26145"
     CDS             complement(304..1017)
                     /locus_tag="NON20_26145"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WLT40677.1"
                     /translation="MDYINGLLPTAVTPWIAVNEYTRESKRLLFNYEKPESCMADLITA
                     IAKGEKVIVHTGGQRASSQWGTINIEKCLKNKFPSLKILRIDAETVSEKGHPAFGIIGN
                     LNTVLPLYDIVIASPTLETGVSIDINHFDRVFGFATGSQTVSAVCQSLARVRADIPRHI
                     WAKPYSNQRIGNGSTDPKLLIKSQQKLFQANLVTLGQTQAIAMDSNNPQHLSTWAHDVS
                     NPEQRLQSIPRIHPR"
                     /name="hypothetical protein"
     gene            complement(1143..2432)
                     /locus_tag="NON20_26150"
     CDS             complement(1143..2432)
                     /locus_tag="NON20_26150"
                     /inference="COORDINATES: protein motif:HMM:NF024367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3854 domain-containing protein"
                     /protein_id="WLT40678.1"
                     /translation="MPTLNTQHLTEWLNSGVSPDLIELNVRSIDTSKPPEENGLGTAIA
                     DDPHQLILYSENLQRHKTGRLSESVLKKYRHLNLGWWCAGLNALTLGSSQWGCFKPDSP
                     RLSQDGKPIKYEHPPKEATELFVLRVTRHIWRTVAKKAKLECPDLEAIANENISREFWQ
                     WVKDNPTVPVIITEGAKKTGSLLSHGYVAIGLPGIYGGYRSKDAEGNPLIKKELIPQLQ
                     VFLQTGREFVFCFDNDPKPSTRKAVRTAIANCGNLLAGEKRTISVMEWRQRVKGIDDLI
                     VADGEGKLDQVFAARVRLEAYKLAPYTDISPLVSHRFDSRYLGNLSAPETAQLIGLKSP
                     KGTGKTEWLAKQAAIAMDQSIPVIVVTHREQLGKELGNRLGLEYRTELTRIGKNLGYVL
                     CIDSLHPKATPHLIQIVGMMPWSSLMKPSR"
                     /name="DUF3854 domain-containing protein"
     gene            complement(2419..2601)
                     /locus_tag="NON20_26155"
     CDS             complement(2419..2601)
                     /locus_tag="NON20_26155"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WLT40679.1"
                     /translation="MSNYTLEARVYKAGEKTSRSPIGELFQAGRLTDLLEFLANHYGIE
                     GDEALAVLGNHHANS"
                     /name="hypothetical protein"
     gene            complement(3119..3319)
                     /locus_tag="NON20_26160"
     CDS             complement(3119..3319)
                     /locus_tag="NON20_26160"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WLT40680.1"
                     /translation="MFVRYEKGMIVEIIEVKKGKMYCSDGRKFDVEIVDCDVWWNGKCV
                     GKIENWDIGFDDNRGLILFGF"
                     /name="hypothetical protein"
     gene            complement(4048..4857)
                     /locus_tag="NON20_26165"
     CDS             complement(4048..4857)
                     /locus_tag="NON20_26165"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="WLT40681.1"
                     /translation="MSATPLLAIRCPSELKTRLEYEAKTSGQSVTRLVLDRIKSGLPSL
                     PLIERKSLPPVACVYFVVGQSNKLLYIGQTQDLLKRWNNHHRLGQLLGIDPECRIAWLE
                     VDDRVAVESSLIEELSPELNGTEGENGFSTTDKTKISAYLDSQDNETLEALAAKHRISK
                     SQVVIMAIRALANSDNVELRPSAKLAPSNGVTLEQVESVIKAAITPLSREIDQLKSPSW
                     WPKIPTDGHQDHQSDSRGSTHRTRIIGTSQHPRHHDPNLGEKVWLKP"
                     /name="GIY-YIG nuclease family protein"
     gene            5026..5262
                     /locus_tag="NON20_26170"
     CDS             5026..5262
                     /locus_tag="NON20_26170"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WLT40682.1"
                     /translation="MLTVQTRPLAKVLHFEGSGDHLTKLDLQHRKTGIPATLFIFAPRP
                     LSIGEVKDSIAKAFDCDRWAYRQLNVTAEYCPF"
                     /name="hypothetical protein"
     gene            5484..5705
                     /locus_tag="NON20_26175"
     CDS             5484..5705
                     /locus_tag="NON20_26175"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WLT40683.1"
                     /translation="MAIALDSKGKYHVVEGQPVKTSAPETEPVGMTAETKRLIAQYVTE
                     QRDLLAFCLDQAATIPRLKLMNRWRNLV"
                     /name="hypothetical protein"
     gene            complement(7304..7753)
                     /locus_tag="NON20_26180"
     CDS             complement(7304..7753)
                     /locus_tag="NON20_26180"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WLT40684.1"
                     /translation="MGRNFVKASDRRRVDKLTEDTGNAFSPDVNGACLTTKVRVMELVN
                     IQQFFQGEHTSESLQSWFNELVKVRRDIKIGLGMSINPERDTPIAVAQRLLGLLGLKMQ
                     GHQHRLNGKRIRTYTLTDDLPPERVELFTRWLERDFARVPFEEIA"
                     /name="hypothetical protein"
     gene            complement(7990..8775)
                     /locus_tag="NON20_26185"
     CDS             complement(7990..8775)
                     /locus_tag="NON20_26185"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WLT40666.1"
                     /translation="MDYINGLLPTAVTPWIAVNEYTRESKRLLFNYEKPESCMADLITA
                     IAKGEKVIVHTGGQRASSQWGTINIEKCLKNKFPSLKILRIDAETVSEKGHPAFGIIGN
                     LNTVLPLYDIVIASPTLETGVSIDINHFDRVFGFATGSQTVSAVCQSLARVRADIPRHI
                     WAKPYSNQRIGNGSTDPKLLIKSQQKLFQANLVTLGQTQAIAMDSNNPQHLSTWATMSA
                     IQNSGFKAYRESILDKLQSEGYEIKDGEILTTEQKLRRT"
                     /name="hypothetical protein"
     gene            complement(8901..10190)
                     /locus_tag="NON20_26190"
     CDS             complement(8901..10190)
                     /locus_tag="NON20_26190"
                     /inference="COORDINATES: protein motif:HMM:NF024367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3854 domain-containing protein"
                     /protein_id="WLT40667.1"
                     /translation="MPTLNTQHLTEWLNSGVSPDLIELNVRSIDTSKPPEENGLGTAIA
                     DDPHQLILYSENLQRHKTGRLSESVLKKYRHLNLGWWCAGLNALTLGSSQWGCFKPDSP
                     RLSQDGKPIKYEHPPKEATELFVLRVTRHIWRTVAKKAKLECPDLEAIANENISREFWQ
                     WVKDNPTVPVIITEGAKKTGSLLSHGYVAIGLPGIYGGYRSKDAEGNPLIKKELIPQLQ
                     VFLQTGREFVFCFDNDPKPSTRKAVRTAIANCGNLLAGEKRTISVMEWRQRVKGIDDLI
                     VADGEGKLDQVFAARVRLEAYKLAPYTDISPLVSHRFDSRYLGNLSAPETAQLIGLKSP
                     KGTGKTEWLAKQAAIAMDQSIPVIVVTHREQLGKELGNRLGLEYRTELTRIGKNLGYVL
                     CIDSLHPKATPHLIQIVGMMPWSSLMKPSR"
                     /name="DUF3854 domain-containing protein"
     gene            complement(10177..10359)
                     /locus_tag="NON20_26195"
     CDS             complement(10177..10359)
                     /locus_tag="NON20_26195"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WLT40668.1"
                     /translation="MSNYTLEARVYKAGEKTSRSPIGELFQAGRLTDLLEFLANHYGIE
                     GDEALAVLGNHHANS"
                     /name="hypothetical protein"
     gene            complement(10876..11076)
                     /locus_tag="NON20_26200"
     CDS             complement(10876..11076)
                     /locus_tag="NON20_26200"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WLT40669.1"
                     /translation="MFVRYEKGMIVEIIEVKKGKMYCSDGRKFDVEIVDCDVWWNGKCV
                     GKIENWDIGFDDNRGLILFGF"
                     /name="hypothetical protein"
     gene            complement(11940..12614)
                     /locus_tag="NON20_26205"
     CDS             complement(11940..12614)
                     /locus_tag="NON20_26205"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="WLT40670.1"
                     /translation="MSATPLLAIRCPSELKTRLEYEAKTSGQSVTRLVLDRIKSGLPSL
                     PLIERKSLPPVACVYFVVGQSNKLLYIGQTQDLLKRWNNHHRLGQLLGIDPECRIAWLE
                     VDDRVAVESSLIEELSPELNGTEGENGFSTTDKTKISAYLDSQDNETLEALAAKHRISK
                     SQVVIMAIRALANSDNVELRPSAKLAPSNGVTLEQVESVIKAAITPLSREIDQLKKPEL
                     VA"
                     /name="GIY-YIG nuclease family protein"
     gene            12783..13019
                     /locus_tag="NON20_26210"
     CDS             12783..13019
                     /locus_tag="NON20_26210"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WLT40671.1"
                     /translation="MLTVQTRPLAKVLHFEGSGDHLTKLDLQHRKTGIPATLFIFAPRP
                     LSIGEVKDSIAKAFDCDRWAYRQLNVTAEYCPF"
                     /name="hypothetical protein"
     gene            13078..13269
                     /locus_tag="NON20_26215"
     CDS             13078..13269
                     /locus_tag="NON20_26215"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WLT40672.1"
                     /translation="MQLLTATVKSQPRTVTTKYGERSVMDVVLPNGEEQSIWGSCQFSS
                     HRQLSQWSIGGDRFGFQR"
                     /name="hypothetical protein"
     gene            13241..13462
                     /locus_tag="NON20_26220"
     CDS             13241..13462
                     /locus_tag="NON20_26220"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WLT40673.1"
                     /translation="MAIALDSKGKYHVVEGQPVKTSAPETEPVGMTAETKRLIAQYVTE
                     QRDLLAFCLDQAATIPRLKLMNRWRNLV"
                     /name="hypothetical protein"
     gene            14543..14743
                     /locus_tag="NON20_26225"
     CDS             14543..14743
                     /locus_tag="NON20_26225"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WLT40674.1"
                     /translation="MLSGVKDVAEVSKPVQGGRRSADESVQEVKKEKKKNYPPHHPDSV
                     QEASPKSVNPVQKKKSRRLQI"
                     /name="hypothetical protein"
ORIGIN
        1 taatggagtc gaagttttgg atataacccc ccatcatcat cggccaccat tgcggggaaa
       61 tctcttgggt ggcataacgt cgggaaattt cggtcttttt ctgagcctaa tctttcttgg
      121 ggggtcttgg ccctggcttt actgacctct tgatattcct tctcggtcag aagcggagaa
      181 ttggccactt cttcacagtg ggacaaataa ctggattcct tggcctcctt catgtccgcc
      241 ttaacttctg ctccgtcgtc aggatctccc ccgtctttga tttcgtagcc ttcgctttgg
      301 agcttatcta ggatggattc tcggtatgct ttgaagccgc tgttctggat tgctgacatc
      361 gtgagcccag gtggaaagat gctgggggtt attggaatcc atggcgatcg cctgagtttg
      421 tccgagggta actagattgg cttggaataa tttttgctgg gacttaatca acaatttcgg
      481 gtcagtggaa ccgttaccaa tgcgctgatt ggagtagggt tttgcccaaa tgtgacgggg
      541 aatatctgct cggactctgg ccaatgactg acagaccgca ctaacagttt gactgccggt
      601 ggcaaatcca aaaactcggt caaagtgatt aatgtcgatg ctcactcccg tttctagggt
      661 tggggaggca atcacgatgt catatagggg taatacagta ttcagattcc ctatgatgcc
      721 aaaggcggga tgcccttttt ctgagacggt ttcggcatca atccgcagga ttttcaggga
      781 tgggaattta tttttcaggc atttctcaat attgatggtt ccccactgac tgctggcccg
      841 ttggccgccg gtgtgaacta taactttttc tcctttggcg atcgccgtaa ttaaatccgc
      901 catgcaggat tcgggctttt cgtagttgaa caggagtctt tttgattccc ttgtatattc
      961 attgactgct atccaagggg tcactgcggt aggtaacaag ccgttgatgt agtccacact
     1021 ggtgcgggtt aaatcggcat cactgaggat gattttggag gcatttccta atagttcggt
     1081 gagggtgcta aggatagcgg gccggttaaa tttgcaggtg tcggagttga gaatgtgcca
     1141 aattacctgc tcggcttcat caaggatgac cagggcatca tgccaacaat ctggattaaa
     1201 tggggggtcg ctttggggtg tagtgagtcg atgcagagga catagcccaa gtttttgcca
     1261 attcgggtta attcggtgcg atattccaag ccaagacgat tcccaagttc tttccccagt
     1321 tgctctcggt gcgtcactac tatgacgggg atactttggt ccatggcgat cgccgcttgt
     1381 ttggctaacc attcagtttt gccggtgcct tttggtgatt taagtccgat taattgggcc
     1441 gtttctgggg ctgagagatt acctaaataa cgggagtcaa agcgatggga aaccagcggg
     1501 gaaatgtcgg tgtagggggc tagtttgtag gcttcgagtc ggactctggc ggcaaaaact
     1561 tggtctagtt tgccttcccc atcggcaacg attaggtcat caatgccttt aactctttgc
     1621 ctccattcca ttactgagat ggtgcgcttt tctccggcta agaggttccc gcagttggcg
     1681 atcgctgttc taactgcttt tctggtgctg ggcttggggt cgttgtcaaa gcagaaaaca
     1741 aattcccgac cggtttggag aaatacttgg agctggggga ttaattcttt tttgattaag
     1801 gggttgccct cggcatcttt ggaacggtaa cccccataaa tgcctggtaa gccaatggcc
     1861 acatatccat ggctgagtaa tgaccctgtt ttcttggccc cttcagtaat gatcaccggc
     1921 acggtggggt tatctttcac ccattgccaa aattccctgg aaatattttc gttggcgatc
     1981 gcctccaggt cggggcattc tagtttggcc tttttggcca ctgttctcca gatgtggcga
     2041 gttacccgca gaacaaataa ttctgttgcc tcttttgggg gatgttcgta tttgatgggc
     2101 ttgccatctt gggagagtcg ggggctgtcg ggcttgaagc atccccattg actgctaccc
     2161 agggtgaggg catttaaccc agcacaccac caacctaggt ttaggtgccg atatttcttc
     2221 aggactgatt cactgagccg gccagttttg tgtcgctgga ggttttctga gtaaaggatg
     2281 agttggtgtg ggtcatcggc gatcgctgtc cctagtccgt tttcttccgg gggcttggat
     2341 gtgtcaatgc tgcgaacatt gagttcgatt aggtcggggc tgactccact attgagccat
     2401 tcggttaggt gctgggtgtt aagagttggc atgatgattt cctaggactg ctagggcttc
     2461 atccccttca atcccgtaat ggtttgctaa aaattcgagt aaatcggtca gccggccggc
     2521 ttggaatagc tctcctattg gcgatcggct agttttttcc cccgccttgt aaacacgggc
     2581 ttcgagggta taattagaca tagcaaagcc tcctctcccc gccggaagga ttacaggccc
     2641 aagcattttg tttgttgacc ccttgctgcg tcccctccta cagttcgaca gcttggggta
     2701 ttcaattttc tgggtttcat gctacgtccc tatcctctac ggggttgttg gctaaatatt
     2761 cggtggtagc cttcctgatt aattcagtcc gggtcatatt cctgcgtctg gcttccgttg
     2821 acaattcttt tccccactct cgcccgtagg ttacgaactt attagtgtgg taatgcttgg
     2881 acatggggac ttctccttaa ctatcttttg taattctacg gtgtgcactc tcagtgtcta
     2941 gagggactcc gtgtttacaa gggaaatttt attttttggg ggtcgatggc tacagaaatc
     3001 catggctcgg aaaatctttt cttggggtgg gtgggttggg gtctgatgtg gggcaatgac
     3061 aaaaaaatca ttgatacctt tcgataccaa tgattaaact caaactcaaa ctaataaact
     3121 aaaaaccaaa taatattaac cccctattat catcaaaacc aatatcccaa ttctcaattt
     3181 ttccaacaca ttttccattc caccaaacat cacaatcaac aatttcaaca tcaaactttc
     3241 ttccatcact acaatacatt ttccctttct taacttcaat tatttcaaca atcattccct
     3301 tttcatatct tacaaacata actaaatcct caataactac gagaacttag gaatggaatg
     3361 tgtcaacccc gaaatagaac caatataaaa tgatgcgaag catctccacc tagtcgttct
     3421 attgagcaag gtttagggtt gactatgtag ttataatgga aaagctgtta ttgatgcgtc
     3481 cactccggtt ttcgtaagta aataacctct cgttcccaaa aaaggcgatc ggcgacaata
     3541 tgcgtgccct tcgctcgaga tgtgtttttg ataaccccct ttttttgtat tccagaagtg
     3601 ctctgggata ctagggccgt tccctttcaa aatcccggca aaaaattaac caaaataggt
     3661 cgtcgattaa gggtgtgata atggagcttc ccacattctt gtgtgttcca gagtcgctca
     3721 gggctgagga tttgcttttg gagtttaggc tcttgggttg gattttcaca gcctaaaccg
     3781 tctttatctc tgtcgagtcg gtggtcgtcg ggcccggtta ctctgactgg ttttttgctg
     3841 ttgggaatat cagagcaatt aatccggcga ttggttggga ggcagtggtc aggatagctg
     3901 gggtggcatt gaccaggctg gggattttgg gtgaggagga gaattaattc aatcattggc
     3961 gatcgcccac cgaataccag agtccatctt tgagttcatg gggcggaatg ggacgggaaa
     4021 atttagctcc tttgggtttg taggattcta aggtttgagc catactttct cccccaggtt
     4081 cggatcgtgg tgccggggat gctgagacgt tccgataatt ctcgttctgt gagtgcttcc
     4141 ccttgagtcg ctttggtgat cttggtgtcc gtcggtgggg attttaggcc accagctcgg
     4201 gctttttagt tggtcaattt ctctggagag tggggttatt gccgctttga tgacggattc
     4261 gacttgctcc aaagtaactc cattgctggg ggctagctta gcgctgggtc gtaactccac
     4321 attgtcgctg tttgctaatg ccctgatggc cataatgaca acttgagatt tactaatgcg
     4381 gtgcttcgcc gccaatgctt ctagtgtctc gttgtcctgg gagtctaggt atgcgctgat
     4441 tttggtcttg tctgtcgtgc tgaaaccatt ctcgccttcc gtcccattta gctctggact
     4501 taactcttca ataaggcttg attcaactgc aacccggtca tctacttcga gccacgctat
     4561 gcgacattcc gggtcaatac ctagcaattg tccgagacgg tggtgattgt tccatctttt
     4621 caaaaggtct tgggtctgac cgatgtataa cagtttgttg ctttgcccta cgacaaagta
     4681 cacgcaagcc acaggaggca aggattttct ctcaatcaac ggcaatgatg gcaaccccga
     4741 tttgatgcgg tcaagtacca gcctagttac gctctgtcct gacgtttttg cttcatattc
     4801 taggcgagtc ttaagctcac tggggcaacg tatcgctaac aatggtgtag cactcattga
     4861 ttactcctgt aaaacactat caatgccagt ctatcaaaga aaagctgaaa taggctaaca
     4921 gaatgatgcc taactgtata acagaatgat ataatagagt tatacagcaa agggggctac
     4981 cgctcttacc tgataacccc ctttcaccta cttgaggcaa tagccatgtt aacagttcaa
     5041 acccgtccgc ttgcgaaggt tctccatttt gagggttctg gtgatcactt aactaagctt
     5101 gatttacagc atcggaaaac tggcattcct gccacgctgt ttatttttgc tcctcgccct
     5161 ctttctattg gggaggttaa ggattcgatt gctaaggctt tcgattgtga ccgttgggct
     5221 tatcggcaac tcaatgtaac tgctgagtat tgccctttct agattgatgg accattaggc
     5281 caccttgagc ccacttcgac acatttacaa aggattaagc catgcaatta cttaccgcta
     5341 ccgtcaaaag tcagccccgc accgtgacca ctaaatatgg tgagcgttcc gttatggatg
     5401 tcgttttacc aaatggggaa gaacaatcca tctgaggccc tgccaatttt cctcccatcg
     5461 ccaattgtcg caatggtcaa tcggtggcga tcgctttgga ttccaaaggt aaatatcatg
     5521 ttgtggaggg tcagcctgtc aaaacttctg cccctgaaac tgagccggtg gggatgactg
     5581 cggaaactaa gcgcttgatt gcccaatatg tgacggagca acgggatttg ttggcgtttt
     5641 gccttgacca ggcggctacg attcccaggc tcaaactgat gaatcggtgg agaaacttgg
     5701 tgtaactctt tacctgagtg cccaacggaa atttaaactc gcttaattaa taaaaagtag
     5761 gtacatcatg gaatcaatcg ctcatggtca ggatgtaaat tcttccccta atactttcgg
     5821 ggggcatgac atttctgagg aggatatcgt ttgtcttcta acgtcacttc cacttaacga
     5881 caagatttat ctatctgctt gtgttttgtt tgagtcgttg gatactttag accgttaccg
     5941 tttcctaagt cgttatcgcc aggagtttat caggtcttag ttattcctac tatttaatga
     6001 ttcgccccgg ttattggctg gggtttttct tgttccccca gtggtggggg ctagggggtc
     6061 aatcaacccc acagtaaacg ctaaatcgtg gcagttgggg actcaattta aacccaataa
     6121 ctcgtactaa tcttgccttg taaacatgga aaaaagatgg tacaatatag ataattggag
     6181 gggcaaaaat gacattaaat gagacgctaa aaacgctaaa agctaaacat cccgacttgc
     6241 aatttgctta ctatccgagc catcgggagc cgaagcttcg ccagtccgtg ataattcgca
     6301 ttcctgacgg taggcatcag aaacccatcg ctgataccct ttacagattg ctttggcaag
     6361 aactgaaaat tccttattat tggcaacctc gggataattg tcttttttgt tgcgtcgtgc
     6421 cccattttta cgattagcgc cacccggcga ccaacccccc tgttcatgtc cggttccccc
     6481 cttggggggt tagggggagg ctccccaacg gggcgggggg gataacacct cgagcgactg
     6541 cgagaccaca tattcttgct ttcttgcttc cgggtgtatt ggtataggga ctgaaataat
     6601 attttttgtc ggtttaggtg tggctgaccc tgtaattccc ccttattcct gagagtttgc
     6661 aggtttttca aagtagttca agggatttta aaccccacca cttaaaatga gaccgccgat
     6721 cttgaccttt ccaccccatc aataaggctg agacgtaagc gggcgaccaa taaataaacg
     6781 ggggtcttgc tttccggggt caaggatgtt gccgaagtca gtaaaccagt gcaaggaggt
     6841 cggcgatcgg cagatgaatc agtgcaagaa gtcaaaaaag aaaaaagaaa aattatcctc
     6901 ctcatcatcc ggattcagtg caagaagctt cgccgaagtc agtaaaccca gtgcaaaaaa
     6961 agaaaagtcg tcgtctccaa atctgagaaa aatctgaatc tccccctggg ggtcgggggg
     7021 gacaaagacc ggattcattc cttagctgtc ttgctttctc acccccactc ggggggtcgc
     7081 cggtcagcat ctatggcaat tttcctaaaa actttcagtc cggcgggggg tggggaggca
     7141 ataacaaaac tggagcggag cgacgaccac aaagtgccgg gaaccagatg tcttgctttc
     7201 ttataatgca gagaaccggg gggtaacaaa agatacgggg atttagattg tgtcacacct
     7261 ctcccaatga cttgcttagt tatacataag aggggtgtga cggttaggcg atttcctcaa
     7321 aaggaacccg ggcaaaatcc ctttctaacc aacgggtaaa aagttcaact cgttctggtg
     7381 ggaggtcatc ggtcagggtg taggtgcgga tccgcttgcc attgagtcgg tgttgatgtc
     7441 cctgcatctt tagccccagt aaaccgagta atctttgagc tacggcgatc ggggtatctc
     7501 gctcaggatt gatgctcatc cccaacccga ttttgatgtc ccggcggact ttgaccagtt
     7561 cattgaacca ggattggagg ctttctgagg tgtgctctcc ttggaagaat tgttgaatgt
     7621 tgaccaactc cattacccgg actttggtgg ttaaacaggc cccgttaaca tcgggactaa
     7681 aggcgtttcc ggtatcctcg gtcagcttat ctaccctccg gcgatcgctg gctttaacaa
     7741 aattcctgcc cacggtgagg aagtaatgga gtcgaagttt tggatataac ccccatcatc
     7801 atcggccacc attgcgggga aatctcttgg gtggcataac gtcgggaaat ttcggtcttt
     7861 ttctgggcca atctttcttg ggggtcttgg ccctggcttt actgacctct tgatattcct
     7921 tctcggtcag aagcggagaa ttggccactt cttcacagtg ggacaaataa ctggattcct
     7981 tggcctcctt catgtccgcc ttaacttctg ctccgtcgtc aggatctccc cgtctttgat
     8041 ttcgtagcct tcgctttgga gcttatctag gatggattct cggtatgctt tgaagccgct
     8101 gttctggatt gctgacatcg tggcccaggt ggaaagatgc tgggggttat tggaatccat
     8161 ggcgatcgcc tgagtttgtc cgagggtaac tagattggct tggaataatt tttgctggga
     8221 cttaatcaac aatttcgggt cagtggaacc gttaccaatg cgctgattgg agtagggttt
     8281 tgcccaaatg tgacggggaa tatctgctcg gactctggcc aatgactgac agaccgcact
     8341 aacagtttga ctgccggtgg caaatccaaa aactcggtca aagtgattaa tgtcgatgct
     8401 cactcccgtt tctagggttg gggaggcaat cacgatgtca tataggggta atacagtatt
     8461 cagattccct atgatgccaa aggcgggatg ccctttttct gagacggttt cggcatcaat
     8521 ccgcaggatt ttcagggatg ggaatttatt tttcaggcat ttctcaatat tgatggttcc
     8581 ccactgactg ctggcccgtt ggccgccggt gtgaactata actttttctc ctttggcgat
     8641 cgccgtaatt aaatccgcca tgcaggattc gggcttttcg tagttgaaca ggagtctttt
     8701 tgattccctt gtatattcat tgactgctat ccaaggggtc actgcggtag gtaacaagcc
     8761 gttgatgtag tccacactgg tgcgggttaa atcggcatca ctgaggatga ttttggaggc
     8821 atttcctaat agttcggtga gggtgctaag gatagcgggc cggttaaatt tgcaggtgtc
     8881 ggagttgaga atgtgccaaa ttacctgctc ggcttcatca aggatgacca gggcatcatg
     8941 ccaacaatct ggattaaatg gggggtcgct ttggggtgta gtgagtcgat gcagaggaca
     9001 tagcccaagt ttttgccaat tcgggttaat tcggtgcgat attccaagcc aagacgattc
     9061 ccaagttctt tccccagttg ctctcggtgc gtcactacta tgacggggat actttggtcc
     9121 atggcgatcg ccgcttgttt ggctaaccat tcagttttgc cggtgccttt tggtgattta
     9181 agtccgatta attgggccgt ttctggggct gagagattac ctaaataacg ggagtcaaag
     9241 cgatgggaaa ccagcgggga aatgtcggtg tagggggcta gtttgtaggc ttcgagtcgg
     9301 actctggcgg caaaaacttg gtctagtttg ccttccccat cggcaacgat taggtcatca
     9361 atgcctttaa ctctttgcct ccattccatt actgagatgg tgcgcttttc tccggctaag
     9421 aggttcccgc agttggcgat cgctgttcta actgcttttc tggtgctggg cttggggtcg
     9481 ttgtcaaagc agaaaacaaa ttcccgaccg gtttggagaa atacttggag ctgggggatt
     9541 aattcttttt tgattaaggg gttgccctcg gcatctttgg aacggtaacc cccataaatg
     9601 cctggtaagc caatggccac atatccatgg ctgagtaatg accctgtttt cttggcccct
     9661 tcagtaatga tcaccggcac ggtggggtta tctttcaccc attgccaaaa ttccctggaa
     9721 atattttcgt tggcgatcgc ctccaggtcg gggcattcta gtttggcctt tttggccact
     9781 gttctccaga tgtggcgagt tacccgcaga acaaataatt ctgttgcctc ttttggggga
     9841 tgttcgtatt tgatgggctt gccatcttgg gagagtcggg ggctgtcggg cttgaagcat
     9901 ccccattgac tgctacccag ggtgagggca tttaacccag cacaccacca acctaggttt
     9961 aggtgccgat atttcttcag gactgattca ctgagccggc cagttttgtg tcgctggagg
    10021 ttttctgagt aaaggatgag ttggtgtggg tcatcggcga tcgctgtccc tagtccgttt
    10081 tcttccgggg gcttggatgt gtcaatgctg cgaacattga gttcgattag gtcggggctg
    10141 actccactat tgagccattc ggttaggtgc tgggtgttaa gagttggcat gatgatttcc
    10201 taggactgct agggcttcat ccccttcaat cccgtaatgg tttgctaaaa attcgagtaa
    10261 atcggtcagc cggccggctt ggaatagctc tcctattggc gatcggctag ttttttcccc
    10321 cgccttgtaa acacgggctt cgagggtata attagacata gcaaagcctc ctctccccgc
    10381 cggaaggatt acaggccaag cattttgttt gttgacccct tgctgcgtcc cctcctacag
    10441 ttcgacagct tggggtattc aattttctgg gtttcatgct acgtccctat cctctacggg
    10501 gttgttggct aaatattcgg tggtagcctt cctgattaat tcagtccggg tcatattcct
    10561 gcgtctggct tccgttgaca attcttttcc ccactctcgc ccgtaggtta cgaacttatt
    10621 agtgtggtaa tgcttggaca tggggacttc tccttaacta tcttttgtaa ttctacggtg
    10681 tgcactctca gtgtctagag ggactccgtg tttacaaggg aaattttatt tttggggggt
    10741 cgatggctac agaaatccat ggctcggaaa atcttttctt ggggtgggtg ggttggggtc
    10801 tgatgtgggg caatgacaaa aaaatcattg atacctttcg ataccaatga ttaaactcaa
    10861 actcaaacta ataaactaaa aaccaaataa tattaacccc ctattatcat caaaaccaat
    10921 atcccaattc tcaatttttc caacacattt tccattccac caaacatcac aatcaacaat
    10981 ttcaacatca aactttcttc catcactaca atacattttc cctttcttaa cttcaattat
    11041 ttcaacaatc attccctttt catatcttac aaacataact aaatcctcaa taactacgag
    11101 aacttaggaa tggaatgtgt caaccccgaa atagaaccaa tataaaatga tgcgaagcat
    11161 ctccacctag tcgttctatt gagcaaggtt tagggttgac tatgtagtta taatggaaaa
    11221 gctgttattg atgcgtccac tccggttttc gtaagtaaat aacctctcgt tcccaaaaaa
    11281 ggcgatcggc gacaatatgc gtgcccttcg ctcgagatgt gtttttgata accccctttt
    11341 tttgtattcc agaagtgctc tgggatacta gggccgttcc ctttcaaaat cccggcaaaa
    11401 aattaaccaa aataggtcgt cgattaaggg tgtgataatg gagcttccca cattcttgtg
    11461 tgttccagag tcgctcaggg ctgaggattt gcttttggag tttaggctct tgggttggat
    11521 tttcacagcc taaaccgtct ttatctctgt cgagtcggtg gtcgtcgggc ccggttactc
    11581 tgactggttt tttgctgttg ggaatatcag agcaattaat ccggcgattg gttgggaggc
    11641 agtggtcagg atagctgggg tggcattgac caggctgggg attttgggtg aggaggagaa
    11701 ttaattcaat cattggcgat cgcccaccga ataccagagt ccatctttga gttcatgggg
    11761 cggaatggga cgggaaaatt tagctccttt gggtttgtag gattctaagg tttggccata
    11821 ctttctcccc caggttcgga tcgtggtgcc ggggatgctg agacgttccg ataattctcg
    11881 ttctgtgagt gcttcccctt gagtcgcttt ggtgatcttg gtgtccgtcg gtggggattt
    11941 taggccacca gctcgggctt ttttagttgg tcaatttctc tggagagtgg ggttattgcc
    12001 gctttgatga cggattcgac ttgctccaaa gtaactccat tgctgggggc tagcttagcg
    12061 ctgggtcgta actccacatt gtcgctgttt gctaatgccc tgatggccat aatgacaact
    12121 tgagatttac taatgcggtg cttcgccgcc aatgcttcta gtgtctcgtt gtcctgggag
    12181 tctaggtatg cgctgatttt ggtcttgtct gtcgtgctga aaccattctc gccttccgtc
    12241 ccatttagct ctggacttaa ctcttcaata aggcttgatt caactgcaac ccggtcatct
    12301 acttcgagcc acgctatgcg acattccggg tcaataccta gcaattgtcc gagacggtgg
    12361 tgattgttcc atcttttcaa aaggtcttgg gtctgaccga tgtataacag tttgttgctt
    12421 tgccctacga caaagtacac gcaagccaca ggaggcaagg attttctctc aatcaacggc
    12481 aatgatggca accccgattt gatgcggtca agtaccagcc tagttacgct ctgtcctgac
    12541 gtttttgctt catattctag gcgagtctta agctcactgg ggcaacgtat cgctaacaat
    12601 ggtgtagcac tcattgatta ctcctgtaaa acactatcaa tgccagtcta tcaaagaaaa
    12661 gctgaaatag gctaacagaa tgatgcctaa ctgtataaca gaatgatata atagagttat
    12721 acagcaaagg gggctaccgc tcttacctga taaccccctt tcacctactt gaggcaatag
    12781 ccatgttaac agttcaaacc cgtccgcttg cgaaggttct ccattttgag ggttctggtg
    12841 atcacttaac taagcttgat ttacagcatc ggaaaactgg cattcctgcc acgctgttta
    12901 tttttgctcc tcgccctctt tctattgggg aggttaagga ttcgattgct aaggctttcg
    12961 attgtgaccg ttgggcttat cggcaactca atgtaactgc tgagtattgc cctttctaga
    13021 ttgatggacc attgggccac cttgacccac ttcgacacat ttacaaagga ttaagccatg
    13081 caattactta ccgctaccgt caaaagtcag ccccgcaccg tgaccactaa atatggtgag
    13141 cgttccgtta tggatgtcgt tttaccaaat ggggaagaac aatccatctg gggctcctgc
    13201 caattttcct cccatcgcca attgtcgcaa tggtcaatcg gtggcgatcg ctttggattc
    13261 caaaggtaaa tatcatgttg tggagggtca gcctgtcaaa acttctgccc ctgaaactga
    13321 gccggtgggg atgactgcgg aaactaagcg cttgattgcc caatatgtga cggagcaacg
    13381 ggatttgttg gcgttttgcc ttgaccaggc ggctacgatt cccaggctca aactgatgaa
    13441 tcggtggaga aacttggtgt aactctttac ctgagtgccc aacggaaatt taaactcgct
    13501 taattaataa aaagtaggta catcatggaa tcaatcgctc atggtcagga tgtaaattct
    13561 tcccctaata ctttcggggg gcatgacatt tctgaggagg atatcgtttg tcttctaacg
    13621 tcacttccac ttaacgacaa gatttatcta tctgcttgtg ttttgtttga gtcgttggat
    13681 actttagacc gttaccgttt cctaagtcgt tatcgccagg agtttatcag gtcttagtta
    13741 ttcctactat ttaatgattc gccccggtta ttggctgggg tttttcttgt tcccccagtg
    13801 gtgggggcta gggggtcaat caaccccaca gtaaacgcta aatcgtggca gttggggact
    13861 caatttaaac ccaataactc gtactaatct tgccttgtaa acatggaaaa aagatggtac
    13921 aatatagata attggagggg caaaaatgac attaaatgag acgctaaaaa cgctaaaagc
    13981 taaacatccc gacttgcaat ttgcttacta tccgagccat cgggagccga agcttcgcca
    14041 gtccgtgata attcgcattc ctgacggtag gcatcagaaa cccatcgctg atacccttta
    14101 cagattgctt tggcaagaac tgaaaattcc ttattattgg caacctcggg ataattgtct
    14161 tttttgttgc gtcgtgcccc atttttacga ttagcgccac ccggcgacca accccctgtt
    14221 catgtccggt tccccccttg gggggttagg gggaggctcc ccaacggggc gggggggata
    14281 acacctcgag cgactgcgag accacatatt cttgctttct tgcttccggg tgtattggta
    14341 tagggactga aataatattt tttgtcggtt taggtgtggc tgaccctgta attccccctt
    14401 attcctgaga gtttgcaggt ttttcaaagt agttcaaggg attttaaacc ccaccactta
    14461 aaatgagacc gccgatcttg acctttccac cccatcaata aggctgagac gtaagcgggc
    14521 gaccaataaa taaacggggg tcttgctttc cggggtcaag gatgttgccg aagtcagtaa
    14581 accagtgcaa ggaggtcggc gatcggcaga tgaatcagtg caagaagtca aaaaagaaaa
    14641 aaagaaaaat tatcctcctc atcatccgga ttcagtgcaa gaagcttcgc cgaagtcagt
    14701 aaacccagtg caaaaaaaga aaagtcgtcg tctccaaatc tgagaaaaat ctgaatctcc
    14761 ccctgggggg tcggggggac aaagaccgga ttcattcctt agctgtcttg ctttctcacc
    14821 cccactcggg gggtcgccgg tcagcatcta tggcaatttt cctaaaaact ttcagtccgg
    14881 cggggggtgg ggaggcaata acaaaactgg agcggagcga cgaccacaaa gtgccgggaa
    14941 ccagatgtct tgctttctta taatgcagag aaccgggggg taacaaaaga tacggggatt
    15001 tagattgtgt cacacctctc ccaatgactt gcttagttat acataagagg ggtgtgacgg
    15061 ttaggcgatt tcctcaaaag gaacccgggc aaaatccctt tctaaccaac gggtaaaaag
    15121 ttcaactcgt tctggtggga ggtcatcggt cagggtgtag gtgcggatcc gcttgccatt
    15181 gagtcggtgt tgatgtccct gcatctttag ccccagtaaa ccgagtaatc tttgagctac
    15241 ggcgatcggg gtatctcgct caggattgat gctcatcccc aacccgattt tgatgtcccg
    15301 gcggactttg accagttcat tgaaccagga ttggaggctt tctgaggtgt gctctccttg
    15361 gaagaattgt tgaatgttga ccaactccat tacccggact ttggtggtta aacaggcccc
    15421 gttaacatcg ggactaaagg cgtttccggt atcctcggtc agcttatcta ccctccggcg
    15481 atcgctggct ttaacaaaat tcctgcccac ggtga
//
