LOCUS       CP101708                3581 bp    DNA     linear   BCT 16-AUG-2023
DEFINITION  Synechocystis sp. B12 plasmid unnamed0.
ACCESSION   CP101708
VERSION     CP101708.1
DBLINK      BioProject: PRJNA860280
            BioSample: SAMN29837452
KEYWORDS    .
SOURCE      Synechocystis sp. B12
  ORGANISM  Synechocystis sp. B12
            Bacteria; Cyanobacteriota; Cyanophyceae; Synechococcales;
            Merismopediaceae; Synechocystis.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Pierotti,D., Mendel,D., Atum,S., Stevani,C. and Freire,R.
  TITLE     Genome sequencing of Synechocystis sp. B12
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Pierotti,D., Mendel,D., Atum,S., Stevani,C. and Freire,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUL-2022) Departamento de Quimica Fundamental,
            Instituto de Quimica - Universidade de Sao Paulo, Av. Professor
            Lineu Prestes, Sao Paulo, Sao Paulo 05508000, Brazil
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 15-JUL-2022
            Assembly Method                   :: Flye v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 128.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 07/25/2022 16:04:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.2
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,290
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 3,954
            CDSs (with protein)               :: 3,954
            Genes (RNA)                       :: 52
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 42
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 1,284
            CDSs (without protein)            :: 1,284
            Pseudo Genes (ambiguous residues) :: 0 of 1,284
            Pseudo Genes (frameshifted)       :: 1,127 of 1,284
            Pseudo Genes (incomplete)         :: 159 of 1,284
            Pseudo Genes (internal stop)      :: 135 of 1,284
            Pseudo Genes (multiple problems)  :: 135 of 1,284
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from Cassius Stevani, Av. Prof.
            Lineu Prestes, 748 B. 8T room 809  05508-000 Sao Paulo, SP -
            Brasil.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Synechocystis sp. B12"
                     /mol_type="genomic DNA"
                     /strain="B12"
                     /isolation_source="mangrove water from the port of Santos,
                     Brazil"
                     /db_xref="taxon:2764086"
                     /plasmid="unnamed0"
                     /geo_loc_name="Brazil: port of Santos"
                     /collection_date="2020-08-19"
     gene            220..543
                     /locus_tag="NON20_00005"
                     /pseudo=""
     CDS             220..543
                     /locus_tag="NON20_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /name="phage holin, lambda family"
     gene            527..1003
                     /locus_tag="NON20_00010"
     CDS             527..1003
                     /locus_tag="NON20_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003796 - lysozyme activity [Evidence IEA]; GO_process:
                     GO:0009253 - peptidoglycan catabolic process [Evidence
                     IEA]; GO_process: GO:0016998 - cell wall macromolecule
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="WLT36771.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTDY
                     SDHPRKLVTLNPKLKSTGAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKERGA
                     LPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIDV"
                     /name="glycoside hydrolase family protein"
     gene            1220..1401
                     /gene="rzoD"
                     /locus_tag="NON20_00015"
                     /pseudo=""
     CDS             1220..1401
                     /gene="rzoD"
                     /locus_tag="NON20_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /name="rzoD"
     gene            complement(1492..1785)
                     /gene="bor"
                     /locus_tag="NON20_00020"
     CDS             complement(1492..1785)
                     /gene="bor"
                     /locus_tag="NON20_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serum resistance lipoprotein Bor"
                     /protein_id="WLT36772.1"
                     /translation="MKKMLLATALALLITGCAQQTFTVQNKPAAVAPKETITHHFFVSG
                     IGQKKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
                     /name="bor"
     gene            complement(2075..2485)
                     /locus_tag="NON20_00025"
     CDS             complement(2075..2485)
                     /locus_tag="NON20_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="WLT36773.1"
                     /translation="MAQVAIFKEIFDQVRKDLDCELFYSELKRHNVSHYIYYLATDNIH
                     IVLENDNTVLIKGLKKVVNVKFSRNTHLIETSYDRLKSREITFQQYRENLAKAGVFRWV
                     TNIHEHKRYYYTFDNSLLFTESIQNTTQIFPR"
                     /name="DUF1398 domain-containing protein"
     gene            2771..2977
                     /locus_tag="NON20_00030"
     CDS             2771..2977
                     /locus_tag="NON20_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WLT36774.1"
                     /translation="MNKEQSADDPSVDLIRVKNMLNSTISMSYPDVVIACIEHKVSLEA
                     FRAIEAALVKHDNNMKDYSLVVD"
                     /name="hypothetical protein"
     gene            complement(3142..3336)
                     /locus_tag="NON20_00035"
     CDS             complement(3142..3336)
                     /locus_tag="NON20_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="WLT36775.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEAC
                     RRRLTSEKRAYTSIKSDEE"
                     /name="YlcI/YnfO family protein"
ORIGIN
        1 agttacgtat tgctgccatc agattgtgtt tgttagtcgc tttttttttt ggaatttttt
       61 tttggaattt tttttgcgct aacaacctcc tgccgttttg cccgtgcata tcggtcacga
      121 acaaatctga ttactaaaca cagtagcctg gatttgttct atcagtaatc gaccttattc
      181 ctaattaaat agagcaaatc cccttattgg gggtaagaca tgaagatgcc agaaaaacat
      241 gacctgttgg ccgccattct cgcggcaaag gaacaaggca tcggggcaat ccttgcgttt
      301 gcaatggcgt accttcgcgg cagatataat ggcggtgcgt ttacaaaaac agtaatcgac
      361 gcaacgatgt gcgccattat cgcctagttc attcgtgacc ttctcgactt cgccggacta
      421 agtagcaatc tcgcttatat aacgagcgtg tttatcggct acatcggtac tgactcgatt
      481 ggttcgctta tcaaacgctt cgctgctaaa aaagccggag tagaagatgg tagaaatcaa
      541 taatcaacgt aaggcgttcc tcgatatgct ggcgtggtcg gagggaactg ataacggacg
      601 tcagaaaacc agaaatcatg gttatgacgt cattgtaggc ggagagctat ttactgatta
      661 ctccgatcac cctcgcaaac ttgtcacgct aaacccaaaa ctcaaatcaa caggcgccgg
      721 acgctaccag cttctttccc gttggtggga tgcctaccgc aagcagcttg gcctgaaaga
      781 cttctctccg aaaagtcagg acgctgtggc attgcagcag attaaggagc gtggcgcttt
      841 acctatgatt gatcgtggtg atatccgtca ggcaatcgac cgttgcagca atatctgggc
      901 ttcactgccg ggcgctggtt atggtcagtt cgagcataag gctgacagcc tgattgcaaa
      961 attcaaagaa gcgggcggaa cggtcagaga gattgatgta tgagcagagt caccgcgatt
     1021 atctccgctc tggttatctg catcatcgtc tgcctgtcat gggctgttaa tcattaccgt
     1081 gataacgcca ttacctacaa agcccagcgc gacaaaaatg ccagagaact gaagctggcg
     1141 aacgcggcaa ttactgacat gcagatgcgt cagcgtgatg ttgctgcgct cgatgcaaaa
     1201 tacacgaagg agttagctga tgctaaagct gaaaatgatg ctctgcgtga tgatgttgcc
     1261 gctggtcgtc gtcggttgca catcaaagca gtctgtcagt cagtgcgtga agccaccacc
     1321 gcctccggcg tggataatgc agcctccccc gactggcaga caccgctgaa cgggattatt
     1381 tcaccctcag agagaggctg atcactatgc aaaaacaact ggaaggaacc cagaagtata
     1441 ttaatgagca gtgcagatag agttgcccat atcgatgggc aactcatgca attattgtga
     1501 gcaatacaca cgcgcttcca gcggagtata aatgcctaaa gtaataaaac cgagcaatcc
     1561 atttacgaat gtttgctggg tttctgtttt aacaacattt tctgcgccgc cacaaatttt
     1621 ggctgcatcg acagttttct tctgcccaat tccagaaacg aagaaatgat gggtgatggt
     1681 ttcctttggt gctactgctg ccggtttgtt ttgaacagta aacgtctgtt gagcacatcc
     1741 tgtaataagc agggccagcg cagtagcgag tagcattttt ttcatggtgt tattcccgat
     1801 gctttttgaa gttcgcagaa tcgtatgtgt agaaaattaa acaaacccta aacaatgagt
     1861 tgaaatttca tattgttaat atttattaat gtatgtcagg tgcgatgaat cgtcattgta
     1921 ttcccggatt aactatgtcc acagccctga cggggaactt ctctgcggga gtgtccggga
     1981 ataattaaaa cgatgcacac agggtttagc gcgtacacgt attgcattat gccaacgccc
     2041 cggtgctgac acggaagaaa ccggacgtta tgatttagcg tggaaagatt tgtgtagtgt
     2101 tctgaatgct ctcagtaaat agtaatgaat tatcaaaggt atagtaatat cttttatgtt
     2161 catggatatt tgtaacccat cggaaaactc ctgctttagc aagattttcc ctgtattgct
     2221 gaaatgtgat ttctcttgat ttcaacctat cataggacgt ttctataaga tgcgtgtttc
     2281 ttgagaattt aacatttaca acctttttaa gtccttttat taacacggtg ttatcgtttt
     2341 ctaacacgat gtgaatatta tctgtggcta gatagtaaat ataatgtgag acgttgtgac
     2401 gttttagttc agaataaaac aattcacagt ctaaatcttt tcgcacttga tcgaatattt
     2461 ctttaaaaat ggcaacctga gccattggta aaaccttcca tgtgatacga gggcgcgtag
     2521 tttgcattat cgtttttatc gtttcaatct ggtctgacct ccttgtgttt tgttgatgat
     2581 ttatgtcaaa tattaggaat gttttcactt aatagtattg gttgcgtaac aaagtgcggt
     2641 cctgctggca ttctggaggg aaatacaacc gacagatgta tgtaaggcca acgtgctcaa
     2701 atcttcatac agaaagattt gaagtaatat tttaaccgct agatgaagag caagcgcatg
     2761 gagcgacaaa atgaataaag aacaatctgc tgatgatccc tccgtggatc tgattcgtgt
     2821 aaaaaatatg cttaatagca ccatttctat gagttaccct gatgttgtaa ttgcatgtat
     2881 agaacataag gtgtctctgg aagcattcag agcaattgag gcagcgttgg tgaagcacga
     2941 taataatatg aaggattatt ccctggtggt tgactgatca ccataactgc taatcattca
     3001 aactatttag tctgtgacag agccaacacg cagtctgtca ctgtcaggaa agtggtaaaa
     3061 ctgcaactca attactgcaa tgccctcgta attaagtgaa tttacaatat cgtcctgttc
     3121 ggagggaaga acgcgggatg ttcattcttc atcactttta attgatgtat atgctctctt
     3181 ttctgacgtt agtctccgac ggcaggcttc aatgacccag gctgagaaat tcccggaccc
     3241 tttttgctca agagcgatgt taatttgttc aatcatttgg ttaggaaagc ggatgttgcg
     3301 ggttgttgtt ctgcgggttc tgttcttcgt tgacatgagg ttgccccgta ttcagtgtcg
     3361 ctgatttgta ttgtctgaag ttgtttttac gttaagttga tgcagatcaa ttaatacgat
     3421 acctgcgtca taattgatta tttgacgtgg tttgatggcc tccacgcacg ttgtgatatg
     3481 tagatgataa tcattatcac tttacgggtc ctttccggtg aaaaaaggta ccaaaaaaac
     3541 atcgtcgtga gtagtgaacc gtaagcagca atacgtaact g
//
