LOCUS       CP100656               10912 bp    DNA     circular BCT 14-SEP-2023
DEFINITION  Salmonella enterica subsp. enterica serovar Alachua strain
            FSIS22029592 plasmid pFSIS22029592-2, complete sequence.
ACCESSION   CP100656
VERSION     CP100656.1
DBLINK      BioProject: PRJNA242847
            BioSample: SAMN16705063
KEYWORDS    .
SOURCE      Salmonella enterica subsp. enterica serovar Alachua
  ORGANISM  Salmonella enterica subsp. enterica serovar Alachua
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 10912)
  AUTHORS   Li,C.
  TITLE     The spread of pESI plasmid through clonal expansion
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10912)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2022) CVM/OR, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 05-JUL-2022
            Assembly Method                   :: HGAP v. 10
            Assembly Name                     :: SMRTLink
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 202x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 07/07/2022 16:24:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,127
            CDSs (total)                      :: 5,007
            Genes (coding)                    :: 4,829
            CDSs (with protein)               :: 4,829
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 65 of 178
            Pseudo Genes (incomplete)         :: 131 of 178
            Pseudo Genes (internal stop)      :: 39 of 178
            Pseudo Genes (multiple problems)  :: 48 of 178
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..10912
                     /organism="Salmonella enterica subsp. enterica serovar
                     Alachua"
                     /mol_type="genomic DNA"
                     /strain="FSIS22029592"
                     /serovar="Salmonella enterica subsp. enterica serovar
                     Alachua"
                     /isolation_source="Comminuted Turkey"
                     /sub_species="enterica"
                     /db_xref="taxon:913240"
                     /plasmid="pFSIS22029592-2"
                     /geo_loc_name="USA:NC"
                     /collection_date="2020"
                     /collected_by="USDA-FSIS"
     gene            281..733
                     /locus_tag="IT836_25565"
                     /pseudo=""
     CDS             281..733
                     /locus_tag="IT836_25565"
                     /inference="COORDINATES: protein motif:HMM:NF015354.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            891..1304
                     /locus_tag="IT836_25570"
     CDS             891..1304
                     /locus_tag="IT836_25570"
                     /inference="COORDINATES: protein motif:HMM:NF036929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="WNA03450.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
                     /name="IncQ-type mobilization protein MobB"
     gene            1403..2272
                     /locus_tag="IT836_25575"
     CDS             1403..2272
                     /locus_tag="IT836_25575"
                     /inference="COORDINATES: protein motif:HMM:NF028103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family DNA primase"
                     /protein_id="WNA03451.1"
                     /translation="MNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDL
                     SEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEYDADPASA
                     DSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQ
                     EKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRS
                     AEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPAPRQRGMD
                     RGGPDFSM"
                     /name="RepB family DNA primase"
     gene            2333..2545
                     /locus_tag="IT836_25580"
     CDS             2333..2545
                     /locus_tag="IT836_25580"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WNA03444.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="hypothetical protein"
     gene            2547..2753
                     /locus_tag="IT836_25585"
     CDS             2547..2753
                     /locus_tag="IT836_25585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WNA03445.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="hypothetical protein"
     gene            2783..3622
                     /locus_tag="IT836_25590"
     CDS             2783..3622
                     /locus_tag="IT836_25590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WNA03446.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            3609..4460
                     /locus_tag="IT836_25595"
     CDS             3609..4460
                     /locus_tag="IT836_25595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="WNA03447.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="replication protein"
     gene            4769..5584
                     /gene="sul2"
                     /locus_tag="IT836_25600"
     ARG             4770..5581
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             6030..6448
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="52.06"
     ARG             5646..6445
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            6448..7284
                     /locus_tag="IT836_25610"
     CDS             6448..7284
                     /locus_tag="IT836_25610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WNA03440.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            7590..7832
                     /locus_tag="IT836_25615"
     CDS             7590..7832
                     /locus_tag="IT836_25615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="WNA03441.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="relaxase"
     gene            complement(7864..8514)
                     /gene="tetR(A)"
                     /locus_tag="IT836_25620"
     CDS             complement(7864..8514)
                     /gene="tetR(A)"
                     /locus_tag="IT836_25620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WNA03449.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            8620..9819
                     /gene="tet(A)"
                     /locus_tag="IT836_25625"
     ARG             8621..9816
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(9786..9974)
                     /locus_tag="IT836_25630"
                     /pseudo=""
     CDS             complement(9786..9974)
                     /locus_tag="IT836_25630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(10572..10856)
                     /locus_tag="IT836_25635"
     CDS             complement(10572..10856)
                     /locus_tag="IT836_25635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="WNA03443.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C"
     ARG             6449..7281
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          2801..3622
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.878"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          144..2269
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggcaatccct cccctacttt gtaggggaga gcgcacttac cggtttctct tcgagaaact
       61 ggcctaacgg ccacccttcg ggcggtgcgc tctccgaggg ccattgcatg gagccgaaaa
      121 gcaaaagcaa cagcgaggca gcatggcgat ttatcacctt acggcgaaaa ccggcagcag
      181 gtcgggcggc caatcggcca gggccaaggc cgactacatc cagcgcgaag gcaagtatgc
      241 ccgcgacatg gatgaagtct tgcacgccga atccgggcac atgccggagt tcgtcgagcg
      301 gcccgccgac tactgggatg ctgccgacct gtatgaacgc gccaatgggc ggctgttcaa
      361 ggaggtcgaa tttgccctgc cggtcgagct gaccctcgac cagcagaagg cgctggcgtc
      421 cgagttcgcc cagcacctga ccggtgccga gcgcctgccg tatacgctgg ccatccatgc
      481 cggtggcggc gagaacccgc actgccacct gatgatctcc gagcggatca atgacggcat
      541 cgagcggccc gccgctcagt ggttcaagcg gtacaacggc aagaccccgg agaagggcgg
      601 ggcacagaag accgaagcgc tcaagcccaa ggcatggctt gagcagaccc gcgaggcatg
      661 ggccgaccat gccaaccggg cattagagcg ggctggccac gacgcccgca ttgaccacag
      721 aacacttgag gcgcagggca tcgagcgcct gcccggtgtt cacctggggc cgaacgtggt
      781 ggagatggaa ggccggggca tccgcaccga ccgggcagac gtggccctga acatcgacac
      841 cgccaacgcc cagatcatcg acttacagga ataccgggag gcaatagacc atgaacgcaa
      901 tcgacagagt gaagaaatcc agaggcatca acgagttagc ggagcagatc gaaccgctgg
      961 cccagagcat ggcgacactg gccgacgaag cccggcaggt catgagccag acccagcagg
     1021 ccagcgaggc gcaggcggcg gagtggctga aagcccagcg ccagacaggg gcggcatggg
     1081 tggagctggc caaagagttg cgggaggtag ccgccgaggt gagcagcgcc gcgcagagcg
     1141 cccggagcgc gtcgcggggg tggcactgga agctatggct aaccgtgatg ctggcttcca
     1201 tgatgcctac ggtggtgctg ctgatcgcat cgttgctctt gctcgacctg acgccactga
     1261 caaccgagga cggctcgatc tggctgcgct tggtggcccg atgaagaacg acaggacttt
     1321 gcaggccata ggccgacagc tcaaggccat gggctgtgag cgcttcgata tcggcgtcag
     1381 ggacgccacc accggccaga tgatgaaccg ggaatggtca gccgccgaag tgctccagaa
     1441 cacgccatgg ctcaagcgga tgaatgccca gggcaatgac gtgtatatca ggcccgccga
     1501 gcaggagcgg catggtctgg tgctggtgga cgacctcagc gagtttgacc tggatgacat
     1561 gaaagccgag ggccgggagc ctgccctggt agtggaaacc agcccgaaga actatcaggc
     1621 atgggtcaag gtggccgacg ccgcaggcgg tgaacttcgg gggcagattg cccggacgct
     1681 ggccagcgag tacgacgccg acccggccag cgccgacagc cgccactatg gccgcttggc
     1741 gggcttcacc aaccgcaagg acaagcacac cacccgcgcc ggttatcagc cgtgggtgct
     1801 gctgcgtgaa tccaagggca agaccgccac cgctggcccg gcgctggtgc agcaggctgg
     1861 ccagcagatc gagcaggccc agcggcagca ggagaaggcc cgcaggctgg ccagcctcga
     1921 actgcccgag cggcagctta gccgccaccg gcgcacggcg ctggacgagt accgcagcga
     1981 gatggccggg ctggtcaagc gcttcggtga tgacctcagc aagtgcgact ttatcgccgc
     2041 gcagaagctg gccagccggg gccgcagtgc cgaggaaatc ggcaaggcca tggccgaggc
     2101 cagcccagcg ctggcagagc gcaagcccgg ccacgaagcg gattacatcg agcgcaccgt
     2161 cagcaaggtc atgggtctgc ccagcgtcca gcttgcgcgg gccgagctgg cacgggcacc
     2221 ggcaccccgc cagcgaggca tggacagggg cgggccagat ttcagcatgt agtgcttgcg
     2281 ttggtactca cgcctgttat actatgagta ctcacgcaca gaagggggtt ttatggaata
     2341 cgaaaaaagc gcttcagggt cggtctacct gatcaaaagt gacaagggct attggttgcc
     2401 cggtggcttt ggttatacgt caaacaaggc cgaggctggc cgcttttcag tcgctgatat
     2461 ggccagcctt aaccttgacg gctgcacctt gtccttgttc cgcgaagaca agcctttcgg
     2521 ccccggcaag tttctcggtg actgatatga aagaccaaaa ggacaagcag accggcgacc
     2581 tgctggccag ccctgacgct gtacgccaag cgcgatatgc cgagcgcatg aaggccaaag
     2641 ggatgcgtca gcgcaagttc tggctgaccg acgacgaata cgaggcgctg cgcgagtgcc
     2701 tggaagaact cagagcggcg cagggcgggg gtagtgaccc cgccagcgcc taaccaccaa
     2761 ctgcctgcaa aggaggcaat caatggctac ccataagcct atcaatattc tggaggcgtt
     2821 cgcagcagcg ccgccaccgc tggactacgt tttgcccaac atggtggccg gtacggtcgg
     2881 ggcgctggtg tcgcccggtg gtgccggtaa atccatgctg gccctgcaac tggccgcaca
     2941 gattgcaggc gggccggatc tgctggaggt gggcgaactg cccaccggcc cggtgatcta
     3001 cctgcccgcc gaagacccgc ccaccgccat tcatcaccgc ctgcacgccc ttggggcgca
     3061 cctcagcgcc gaggaacggc aagccgtggc tgacggcctg ctgatccagc cgctgatcgg
     3121 cagcctgccc aacatcatgg ccccggagtg gttcgacggc ctcaagcgcg ccgccgaggg
     3181 ccggcgcctg atggtgctgg acacgctgcg ccggttccac atcgaggaag aaaacgccag
     3241 cggccccatg gcccaggtca tcggtcgcat ggaggccatc gccgccgata ccgggtgctc
     3301 tatcgtgttc ctgcaccatg ccagcaaggg cgcggccatg atgggcgcag gcgaccagca
     3361 gcaggccagc cggggcagct cggtactggt cgataacatc cgctggcagt cctacctgtc
     3421 gagcatgacc agcgccgagg ccgaggaatg gggtgtggac gacgaccagc gccggttctt
     3481 cgtccgcttc ggtgtgagca aggccaacta tggcgcaccg ttcgctgatc ggtggttcag
     3541 gcggcatgac ggcggggtgc tcaagcccgc cgtgctggag aggcagcgca agagcaaggg
     3601 ggtgccccgt ggtgaagcct aagaacaagc acagcctcag ccacgtccgg cacgacccgg
     3661 cgcactgtct ggcccccggc ctgttccgtg ccctcaagcg gggcgagcgc aagcgcagca
     3721 agctggacgt gacgtatgac tacggcgacg gcaagcggat cgagttcagc ggcccggagc
     3781 cgctgggcgc tgatgatctg cgcatcctgc aagggctggt ggccatggct gggcctaatg
     3841 gcctagtgct tggcccggaa cccaagaccg aaggcggacg gcagctccgg ctgttcctgg
     3901 aacccaagtg ggaggccgtc accgctgatg ccatggtggt caaaggtagc tatcgggcgc
     3961 tggcaaagga aatcggggca gaggtcgata gtggtggggc gctcaagcac atacaggact
     4021 gcatcgagcg cctttggaag gtatccatca tcgcccagaa tggccgcaag cggcaggggt
     4081 ttcggctgct gtcggagtac gccagcgacg aggcggacgg gcgcctgtac gtggccctga
     4141 accccttgat cgcgcaggcc gtcatgggtg gcggccagca tgtgcgcatc agcatggacg
     4201 aggtgcgggc gctggacagc gaaaccgccc gcctgctgca ccagcggctg tgtggctgga
     4261 tcgaccccgg caaaaccggc aaggcttcca tagatacctt gtgcggctat gtctggccgt
     4321 cagaggccag tggttcgacc atgcgcaagc gccgccagcg ggtgcgcgag gcgttgccgg
     4381 agctggtcgc gctgggctgg acggtaaccg agttcgcggc gggcaagtac gacatcaccc
     4441 ggcccaaggc ggcaggctga ccccccccca ctctattgta aacaagacat ttttatcttt
     4501 tatattcaat ggcttatttt cctgctaatt ggtaatacca tgaaaaatac catgctcaga
     4561 aaaggcttaa caatattttg aaaaattgcc tactgagcgc tgccgcacag ctccataggc
     4621 cgctttcctg gctttgcttc cagatgtatg ctcttctgct cctgcagcta atggatcacc
     4681 gcaaacaggt tactcgcctg gggattccct ttcgacccga gcatccgtat gagactcatg
     4741 ctcgattatt attattatag aagcccccat gaataaatcg ctcatcattt tcggcatcgt
     4801 caacataacc tcggacagtt tctccgatgg aggccggtat ctggcgccag acgcagccat
     4861 tgcgcaggcg cgtaagctga tggccgaggg ggcagatgtg atcgacctcg gtccggcatc
     4921 cagcaatccc gacgccgcgc ctgtttcgtc cgacacagaa atcgcgcgta tcgcgccggt
     4981 gctggacgcg ctcaaggcag atggcattcc cgtctcgctc gacagttatc aacccgcgac
     5041 gcaagcctat gccttgtcgc gtggtgtggc ctatctcaat gatattcgcg gttttccaga
     5101 cgctgcgttc tatccgcaat tggcgaaatc atctgccaaa ctcgtcgtta tgcattcggt
     5161 gcaagacggg caggcagatc ggcgcgaggc acccgctggc gacatcatgg atcacattgc
     5221 ggcgttcttt gacgcgcgca tcgcggcgct gacgggtgcc ggtatcaaac gcaaccgcct
     5281 tgtccttgat cccggcatgg ggttttttct gggggctgct cccgaaacct cgctctcggt
     5341 gctggcgcgg ttcgatgaat tgcggctgcg cttcgatttg ccggtgcttc tgtctgtttc
     5401 gcgcaaatcc tttctgcgcg cgctcacagg ccgtggtccg ggggatgtcg gggccgcgac
     5461 actcgctgca gagcttgccg ccgccgcagg tggagctgac ttcatccgca cacacgagcc
     5521 gcgccccttg cgcgacgggc tggcggtatt ggcggcgctg aaagaaaccg caagaattcg
     5581 ttaactgcac attcgggata tttctctata ttcgcgcttc atcagaaaac tgaaggaacc
     5641 tccattgaat cgaactaata ttttttttgg tgaatcgcat tctgactggt tgcctgtcag
     5701 aggcggagaa tctggtgatt ttgtttttcg acgtggtgac gggcatgcct tcgcgaaaat
     5761 cgcacctgct tcccgccgcg gtgagctcgc tggagagcgt gaccgcctca tttggctcaa
     5821 aggtcgaggt gtggcttgcc ccgaggtcat caactggcag gaggaacagg agggtgcatg
     5881 cttggtgata acggcaattc cgggagtacc ggcggctgat ctgtctggag cggatttgct
     5941 caaagcgtgg ccgtcaatgg ggcagcaact tggcgctgtt cacagcctat cggttgatca
     6001 atgtccgttt gagcgcaggc tgtcgcgaat gttcggacgc gccgttgatg tggtgtcccg
     6061 caatgccgtc aatcccgact tcttaccgga cgaggacaag agtacgccgc agctcgatct
     6121 tttggctcgt gtcgaacgag agctaccggt gcggctcgac caagagcgca ccgatatggt
     6181 tgtttgccat ggtgatccct gcatgccgaa cttcatggtg gaccctaaaa ctcttcaatg
     6241 cacgggtctg atcgaccttg ggcggctcgg aacagcagat cgctatgccg atttggcact
     6301 catgattgct aacgccgaag agaactgggc agcgccagat gaagcagagc gcgccttcgc
     6361 tgtcctattc aatgtattgg ggatcgaagc ccccgaccgc gaacgccttg ccttctatct
     6421 gcgattggac cctctgactt ggggttgatg ttcatgccgc ctgtttttcc tgctcattgg
     6481 cacgtttcgc aacctgttct cattgcggac accttttcca gcctcgtttg gaaagtttca
     6541 ttgccagacg ggactcctgc aatcgtcaag ggattgaaac ctatagaaga cattgctgat
     6601 gaactgcgcg gggccgacta tctggtatgg cgcaatggga ggggagcagt ccggttgctc
     6661 ggtcgtgaga acaatctgat gttgctcgaa tatgccgggg agcgaatgct ctctcacatc
     6721 gttgccgagc acggcgacta ccaggcgacc gaaattgcag cggaactaat ggcgaagctg
     6781 tatgccgcat ctgaggaacc cctgccttct gcccttctcc cgatccggga tcgctttgca
     6841 gctttgtttc agcgggcgcg cgatgatcaa aacgcaggtt gtcaaactga ctacgtccac
     6901 gcggcgatta tagccgatca aatgatgagc aatgcctcgg aactgcgtgg gctacatggc
     6961 gatctgcatc atgaaaacat catgttctcc agtcgcggct ggctggtgat agatcccgtc
     7021 ggtctggtcg gtgaagtggg ctttggcgcc gccaatatgt tctacgatcc ggctgacaga
     7081 gacgaccttt gtctcgatcc tagacgcatt gcacagatgg cggacgcatt ctctcgtgcg
     7141 ctggacgtcg atccgcgtcg cctgctcgac caggcgtacg cttatgggtg cctttccgca
     7201 gcttggaacg cggatggaga agaggagcaa cgcgatctag ctatcgcggc cgcgatcaag
     7261 caggtgcgac agacgtcata ctagatatca agcgacttct cctatcccct gggaacacat
     7321 caatctcacc ggagaatatc gctggccaaa gccttagcgt aggattccgc cccttcccgc
     7381 aaacgacccc tggaagcccc gtagcgacgc ggagaggggc gagacaagcc aagggcgcag
     7441 gctcgatgcg cagcacgaca tagccggttc tcgcaaggac gagaatttcc ctgcggtgcc
     7501 cctcaagtgt caatgaaagt ttccaacgcg agccattcgc gagagccttg agtccacgct
     7561 agatctatct catctgcgca aggcagaacg tgaagacggc cgccctggac ctcgcccgcg
     7621 agcgccaggc gcacgaggcc ggcgcgcgga cccgcgccac ggcccacgag cggacgccgc
     7681 agcaggagcg ccagaaggcc gccagagagg ccgagcgcgg ccgtgaggct tggacgctag
     7741 ggcagggcat gaaaaagccc gtagcgggct gctacgggcg tctgacgcgg tggaaagggg
     7801 gaggggatgt tgtctacatg gctctgctgt agtgagtggg ttgcgctccg gcagcggtcc
     7861 tgatcaatcg tcaccctttc tcggtccttc aacgttcctg acaacgagcc tccttttcgc
     7921 caatccatcg acaatcaccg cgagtccctg ctcgaacgct gcgtccggac cggcttcgtc
     7981 gaaggcgtct atcgcggccc gcaacagcgg cgagagcgga gcctgttcaa cggtgccgcc
     8041 gcgctcgccg gcatcgctgt cgccggcctg ctcctcaagc acggccccaa cagtgaagta
     8101 gctgattgtc atcagcgcat tgacggcgtc cccggccgaa aaacccgcct cgcagaggaa
     8161 gcgaagctgc gcgtcggccg tttccatctg cggtgcgccc ggtcgcgtgc cggcatggat
     8221 gcgcgcgcca tcgcggtagg cgagcagcgc ctgcctgaag ctgcgggcat tcccgatcag
     8281 aaatgagcgc cagtcgtcgt cggctctcgg caccgaatgc gtatgattct ccgccagcat
     8341 ggcttcggcc agtgcgtcga gcagcgcccg cttgttcctg aagtgccagt aaagcgccgg
     8401 ctgctgaacc cccaaccgtt ccgccagttt gcgtgtcgtc agaccgtcta cgccgacctc
     8461 gttcaacagg tccagggcgg cacggatcac tgtattcggc tgcaactttg tcatgcttga
     8521 cactttatca ctgataaaca taatatgtcc accaacttat cagtgataaa gaatccgcgc
     8581 gttcaatcgg accagcggag gctggtccgg aggccagacg tgaaacccaa cagacccctg
     8641 atcgtaattc tgagcactgt cgcgctcgac gctgtcggca tcggcctgat tatgccggtg
     8701 ctgccgggcc tcctgcgcga tctggttcac tcgaacgacg tcaccgccca ctatggcatt
     8761 ctgctggcgc tgtatgcgtt gatgcaattt gcctgcgcac ctgtgctggg cgcgctgtcg
     8821 gatcgtttcg ggcggcggcc ggtcttgctc gtctcgctgg ccggcgctgc tgtcgactac
     8881 gccatcatgg cgacggcgcc tttcctttgg gttctctata tcgggcggat cgtggccggc
     8941 atcaccgggg cgactggggc ggtagccggc gcttatattg ccgatatcac tgatggcgat
     9001 gagcgcgcgc ggcacttcgg cttcatgagc gcctgtttcg ggttcgggat ggtcgcggga
     9061 cctgtgctcg gtgggctgat gggcggtttc tccccccacg ctccgttctt cgccgcggca
     9121 gccttgaacg gcctcaattt cctgacgggc tgtttccttt tgccggagtc gcacaaaggc
     9181 gaacgccggc cgttacgccg ggaggctctc aacccgctcg cttcgttccg gtgggcccgg
     9241 ggcatgaccg tcgtcgccgc cctgatggcg gtcttcttca tcatgcaact tgtcggacag
     9301 gtgccggccg cgctttgggt cattttcggc gaggatcgct ttcactggga cgcgaccacg
     9361 atcggcattt cgcttgccgc atttggcatt ctgcattcac tcgcccaggc aatgatcacc
     9421 ggccctgtag ccgcccggct cggcgaaagg cgggcactca tgctcggaat gattgccgac
     9481 ggcacaggct acatcctgct tgccttcgcg acacggggat ggatggcgtt cccgatcatg
     9541 gtcctgcttg cttcgggtgg catcggaatg ccggcgctgc aagcaatgtt gtccaggcag
     9601 gtggatgagg aacgtcaggg gcagctgcaa ggctcactgg cggcgctcac cagcctgacc
     9661 tcgatcgtcg gacccctcct cttcacggcg atctatgcgg cttctataac aacgtggaac
     9721 gggtgggcat ggattgcagg cgctgccctc tacttgctct gcctgccggc gctgcgtcgc
     9781 gggctttgga gaaattcttc aaattcccgt tgcacatagc ccggcaattc ctttccctgc
     9841 tctgccataa gcgcagcgaa tgccgggtaa tactcgtcaa cgatctgata gagaagggtt
     9901 tgctcgggtc ggtggctctg gtaacgacca gtatcccgat cccggctggc cgtcctggcc
     9961 gccacatgag gcatgttccg cgtccttgca atactgtgtt tacatacagt ctatcgctta
    10021 gcggaaagtt cttttaccct cagccgaaat gcctgccgtt gctagacatt gccagccagt
    10081 gcccgtcact cccgtactaa ctgtcacgaa cccctgcaat aactgtcacg cccccctgca
    10141 ataactgtca cgaacccctg caataactgt cacgcccccc tgcaataact gtcacgaacc
    10201 cctgcaataa ctgtcacgcc cccaaacctg caaacccagc aggggcgggg gctggcgggg
    10261 tgttggaaaa atccatccat gattatctaa gaataatcca ctaggcgcgg ttatcagcgc
    10321 ccttgtgggg cgctgctgcc cttgcccaat atgcccggcc agaggccgga tagctggtct
    10381 attcgctgcg ctaggctaca caccgcccca ccgctgcgcg gcagggggaa aggcgggcaa
    10441 agcccgctaa accccacacc aaaccccgca gaaatacgct ggagcgcttt tagccgcttt
    10501 agcggccttt ccccctaccc gaagggtggg ggcgcgtgtg cagccccgca gggcctgtct
    10561 cggtcgatca ttcagcccgg ctcatccttc tggcgtggcg gcagaccgaa caaggcgcgg
    10621 tcgtggtcgc gttcaaggta cgcatccatt gccgccatga gccgatcctc cggccactcg
    10681 ctgctgttca ccttggccaa aatcatggcc cccaccagca ccttgcgcct tgtttcgttc
    10741 ttgcgctctt gctgctgttc ccttgcccgc acccgctgaa tttcggcatt gattcgcgct
    10801 cgttgttctt cgagcttggc cagccgatcc gccgccttgt tgctcccctt aaccatcttg
    10861 acaccccatt gttaatgtgc tgtctcgtag gctatcatgg aggcacagcg gc
//
