LOCUS       CP100048                8219 bp    DNA     circular BCT 19-SEP-2023
DEFINITION  Shigella flexneri strain 2457T plasmid p2457T_8, complete sequence.
ACCESSION   CP100048
VERSION     CP100048.1
DBLINK      BioProject: PRJNA851422
            BioSample: SAMN29224628
KEYWORDS    .
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8219)
  AUTHORS   Gabor,C., DeLaine,B., Hazen,T.H., Vyas,G., Mehta,A., Barry,E. and
            Rasko,D.A.
  TITLE     Complete genomes of Shigella flexneri archetype strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8219)
  AUTHORS   Hazen,T.H., Vyas,G., Mehta,A., Barry,E. and Rasko,D.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUN-2022) Institute for Genome Sciences, University of
            Maryland, School of Medicine, 670 W. Baltimore St., Baltimore, MD
            21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 11.3x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/29/2022 23:13:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,143
            CDSs (total)                      :: 5,010
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 133
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 103
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 1,019
            CDSs (without protein)            :: 1,019
            Pseudo Genes (ambiguous residues) :: 0 of 1,019
            Pseudo Genes (frameshifted)       :: 569 of 1,019
            Pseudo Genes (incomplete)         :: 435 of 1,019
            Pseudo Genes (internal stop)      :: 177 of 1,019
            Pseudo Genes (multiple problems)  :: 151 of 1,019
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8219
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /strain="2457T"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /plasmid="p2457T_8"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.67 N 139.65 E"
                     /collection_date="1954"
     gene            join(7801..8219,1..112)
                     /locus_tag="NKG97_25660"
     CDS             join(7801..8219,1..112)
                     /locus_tag="NKG97_25660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WNE54485.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSG
                     GTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETNWTM
                     ENGKQNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            119..352
                     /locus_tag="NKG97_25665"
     CDS             119..352
                     /locus_tag="NKG97_25665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WNE54486.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(897..1289)
                     /locus_tag="NKG97_25670"
     CDS             complement(897..1289)
                     /locus_tag="NKG97_25670"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WNE54487.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQI
                     QEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGNNA
                     ETQSTHYQPYPYDKVSIWGPFPHNLT"
                     /name="hypothetical protein"
     gene            complement(2434..2625)
                     /locus_tag="NKG97_25675"
     CDS             complement(2434..2625)
                     /locus_tag="NKG97_25675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WNE54488.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2690..3013
                     /locus_tag="NKG97_25680"
     CDS             2690..3013
                     /locus_tag="NKG97_25680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WNE54489.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3222..3653
                     /locus_tag="NKG97_25685"
                     /pseudo=""
     CDS             3222..3653
                     /locus_tag="NKG97_25685"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3691..4221
                     /locus_tag="NKG97_25690"
     CDS             3691..4221
                     /locus_tag="NKG97_25690"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WNE54490.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSG
                     GTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETNWTM
                     ENGKQNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            4228..4461
                     /locus_tag="NKG97_25695"
     CDS             4228..4461
                     /locus_tag="NKG97_25695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WNE54481.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5007..5399)
                     /locus_tag="NKG97_25700"
     CDS             complement(5007..5399)
                     /locus_tag="NKG97_25700"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WNE54482.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQI
                     QEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGNNA
                     ETQSTHYQPYPYDKVSIWGPFPHNLT"
                     /name="hypothetical protein"
     gene            complement(6544..6735)
                     /locus_tag="NKG97_25705"
     CDS             complement(6544..6735)
                     /locus_tag="NKG97_25705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WNE54483.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6800..7123
                     /locus_tag="NKG97_25710"
     CDS             6800..7123
                     /locus_tag="NKG97_25710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WNE54484.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            7332..7763
                     /locus_tag="NKG97_25715"
                     /pseudo=""
     CDS             7332..7763
                     /locus_tag="NKG97_25715"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          2261..2347
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.43e-38"
     Typing          6371..6457
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.43e-38"
     Typing          complement(1693..2250)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.032"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5803..6360)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.032"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3004..4565
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.505"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7114..8219
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.103"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcacgagggg agcaacggac gttttctggt gttgcccgag gggatgaaag ccgaaacgaa
       61 ctggacgatg gaaaacggga aacagaatgc cgtaaggctg gtacaggagt gacagagcat
      121 gacggagctg gaaagccatt tgctgaacgc attagagcag ctgcaacagg actatatgca
      181 gcggctgaac gaatgggaga gcgccttcgt ggaattgcag aaaatgtttt cgcttacgca
      241 acgggacaac gcgatgctga acgagcgggt catgcagttg agtcagcagg tgctgcactt
      301 gagcgagcag acagaacgct tgagccagtt atacagcgag aactggagat aagagaggaa
      361 cggttgatac aggagccgaa catgcgttat tcctggaacg ggaacgccag ccggaaatac
      421 aggaacggac actggatggc ccttcgctgg gatggtaatc agagacaggc taaaatgttg
      481 tcagcgcgtt tccggtgcat tttttgggat aatggttggt tcgggggcac cgggaacgcg
      541 cttcagtcag accttgctga aaacacagga acgagttaaa acgcatacgg gcgcgattct
      601 ggtgtgtctt ttgtcgctgt gttttgcctc tattgccaga tcgcgctcat tcccctgaac
      661 ttctgttttc ccgctcaggt ccccgtcttc ttcgcttccg aaagcatgaa taaaatccgt
      721 ttcagctgtc tctggctggc aattcctgcg tgattcacat ggctgcatag ctatgcatgt
      781 ggttaaaatt taccgggtgc gatcgcggca gtttttcggg tggtttgttg ccatttttac
      841 ctgtctgctg ccgtgatcgc gccgaacgcg tttcagcggt gcgtacaatt aagatattac
      901 gttaaattgt gagggaatgg tccccatata ctgactttat catagggata gggttggtag
      961 tgggtcgatt gggtttcggc attgtttccg ttaatgccaa ttgcattttt gtatttgatg
     1021 caacagaagg cgtgagattc tcctgggcct ggaaaggcta agtgtttaga attgatggca
     1081 aaatatcttc cgtccgattt ttgaagtagt tgttgtttgg ctgtctccca aagggtgctg
     1141 ttacaaactt cttgaatttg tagtttttgc aggactctta ttactgcgtt gtcatgttcc
     1201 aattctttag gggagtgtat tattctctct tttaataagc tgtttaccac agggtttagt
     1261 tgttgtctaa agcacgcaga taatgccatt gctacacaat tttgttcccg aggaagcatg
     1321 tttggcgggg tatgccgtgt ttgatgtgat atgtctattc tcatagaagg tctctggctt
     1381 atagcatcaa caaatacggc tataaaacaa aattttcata gagtgattca agatcttctt
     1441 gagatccttt tttgcggcgc gtaatctgct gcctgcaaac aaaaaaacca ccgctaccaa
     1501 cggtggtttg tttgccggaa tctagagcta ccaactcttt cttccgaagg taactggctt
     1561 ggtggagcac agataccaaa tgctgtcctt ctagtgtagc cgcagttagg ccaccacttc
     1621 aagaactctt aatatctcaa tccaccttgt ccagttacca atggctgctg ccagtggcgt
     1681 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     1741 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     1801 gagataccaa cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     1861 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     1921 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcggtt
     1981 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccatcggcg cggccttctt
     2041 cctgttcctg gtcttttgct cacatgttct ttccggcgtt atcccctgat tctgtggata
     2101 acagcatttc gtttttgagt gagtagatac cgctcgccgc agtcgaacga ccgagcgtag
     2161 cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg cattttctcc ttacgcatct
     2221 gtgcggcatt tcacacccgg tatggcgcac ttttcataca atccgcactg atgccgcatg
     2281 gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac
     2341 ccgctaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgctcacagc
     2401 ttatctgtga gcggatgccg ggagctgcat gcgtcagagg ttttcaccgt catccccgaa
     2461 gcgagcgagg caactgcggt aaagctcatc ggcgtggtcg taaagcgatt cacagatgtc
     2521 tgcctgttca tccgcgtcca gttcgttgag cttctccagc agcgttaatg tctggcttct
     2581 gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta
     2641 agggggattt ctgttcatgg gggtaatgat accgatgaaa cgcgagagga tgctcacgat
     2701 acgggttact gatgatgaac atgcccggtt actggaacgc tgtgagggta aacagctggc
     2761 ggtatggatg cggcgggtct gcctgggtga gccggttgcc cgttccggga ggctgccgac
     2821 actggcaccg ccgttactgc gtcagctggc cgccatcggg aataacctga atcaggcagc
     2881 gaggaaggtg aacagcgggc agtggtcttc cggtgaccgg gttcaggtgg tggccgcact
     2941 gatggccatc ggggatgagc tgcgccggct gcgtctggcc gtcagggaac aggggacgcg
     3001 ggatgatagt taagtttcat gccaggggaa aaggcggtgg cagtggtccg gttgattacc
     3061 tgctgggaag ggagcgtaac cgggaagggg caacggtgct tcagggtaat ccggaagaag
     3121 tccgggaact catcgatgcc acgccatttg cgaagaaata cacgtccggt gttctctcgt
     3181 tcgcggagaa ggagctgccg ccgggagggc gtgaaaaagt gatggcgagc tttgagcgtg
     3241 tactgatgcc cggtctcgaa aaaaatcagt acagcattct gtgggtggag caccaggaca
     3301 aggggcggct tgagctgaat tttgtcattc cgaacatgga gctacagacc ggaaaacgcc
     3361 tccagccgta ctacgaccgc gcagacaggc cgagaattga tgcctggcag acgctggtga
     3421 atcaccatta cgggctgcat gacccgaacg ccccggagaa ccgccggacg ctgactctcc
     3481 ctgataacct gccggaaacg aaacaggcgc ttgctgagag cattacacgg tggatagacg
     3541 ctctttatca tgtcggggag ataaaaggcc gtcaggatgt gattcaggcg ctcacagagg
     3601 cggggcttga agtggtcagg gtgacgcgaa gcagtatcag cattgcagac ccgaacggcg
     3661 ggaagaatat cagactgaaa ggagcattct atgagcaatc ttttacagac gggcgcggag
     3721 ttcgagaaaa agctgaaaga gagagccgaa tctacagaga aaatgctgag cgacgagttc
     3781 aggaagctcg aagaatctgt aagcaaggct gtgacatcaa acgagacgaa aatcaaagac
     3841 gctatagccc tgttcacagc ttcgacagag gaatcactga aaaaacaccg ggaaggggtg
     3901 aaagaggcga tgatgcagca caggaaggac gtgttaaagc tggcagggaa tacgggcatg
     3961 atgttactgg ggatggtcat tttcctgttt accgtgagtg gcgggacgct ctggtatctt
     4021 ggcgggatga tacaggcgaa cctggaagaa atcaggaagc agaacgagag tctggagaag
     4081 ctgaacgcga agacatgggg cgtgcgttat cacgagggga gcaacggacg ttttctggtg
     4141 ttgcccgagg ggatgaaagc cgaaacgaac tggacgatgg aaaacgggaa acagaatgcc
     4201 gtaaggctgg tacaggagtg acagagcatg acggagctgg aaagccattt gctgaacgca
     4261 ttagagcagc tgcaacagga ctatatgcag cggctgaacg aatgggagag cgccttcgtg
     4321 gaattgcaga aaatgttttc gcttacgcaa cgggacaacg cgatgctgaa cgagcgggtc
     4381 atgcagttga gtcagcaggt gctgcacttg agcgagcaga cagaacgctt gagccagtta
     4441 tacagcgaga actggagata agagaggaac ggttgataca ggagcgcgaa catgcgttat
     4501 tcctggaacg ggaacgccag ccggaaatac aggaacggac actggatggc ccttcgctgg
     4561 gatggtaatc agagacaggc taaaatgttg tcagcgcgtt tccggtgcat tttttgggat
     4621 aatggttggt tcgggggcac cgggaacgcg cttcagtcag accttgctga aaacacagga
     4681 acgagttaaa acgcatacgg gcgcgattct ggtgtgtctt ttgtcgctgt gttttgcctc
     4741 tattgccaga tcgcgctcat tcccctgaac ttctgttttc ccgctcaggt ccccgtcttc
     4801 ttcgcttccg aaagcatgaa taaaatccgt ttcagctgtc tctggctggc aattcctgcg
     4861 tgattcacat ggctgcatag ctatgcatgt ggttaaaatt taccgggtgc gatcgcggca
     4921 gtttttcggg tggtttgttg ccatttttac ctgtctgctg ccgtgatcgc gccgaacgcg
     4981 tttcagcggt gcgtacaatt aagatattac gttaaattgt gagggaatgg tccccatata
     5041 ctgactttat catagggata gggttggtag tgggtcgatt gggtttcggc attgtttccg
     5101 ttaatgccaa ttgcattttt gtatttgatg caacagaagg cgtgagattc tcctgggcct
     5161 ggaaaggcta agtgtttaga attgatggca aaatatcttc cgtccgattt ttgaagtagt
     5221 tgttgtttgg ctgtctccca aagggtgctg ttacaaactt cttgaatttg tagtttttgc
     5281 aggactctta ttactgcgtt gtcatgttcc aattctttag gggagtgtat tattctctct
     5341 tttaataagc tgtttaccac agggtttagt tgttgtctaa agcacgcaga taatgccatt
     5401 gctacacaat tttgttcccg aggaagcatg tttggcgggg tatgccgtgt ttgatgtgat
     5461 atgtctattc tcatagaagg tctctggctt atagcatcaa caaatacggc tataaaacaa
     5521 aattttcata gagtgattca agatcttctt gagatccttt tttgcggcgc gtaatctgct
     5581 gcctgcaaac aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta
     5641 ccaactcttt cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt
     5701 ctagtgtagc cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt
     5761 ccagttacca atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag
     5821 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     5881 cagcttggag cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag
     5941 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     6001 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     6061 gtttcgccac ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct
     6121 atggaaaaac gccatcggcg cggccttctt cctgttcctg gtcttttgct cacatgttct
     6181 ttccggcgtt atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac
     6241 cgctcgccgc agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg
     6301 cctggacgtg cattttctcc ttacgcatct gtgcggcatt tcacacccgg tatggcgcac
     6361 ttttcataca atccgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta
     6421 cgtgactggg tcatggctgc gccccgacac ccgctaacac ccgctgacgc gccctgacgg
     6481 gcttgtctgc tcccggcatc cgctcacagc ttatctgtga gcggatgccg ggagctgcat
     6541 gcgtcagagg ttttcaccgt catccccgaa gcgagcgagg caactgcggt aaagctcatc
     6601 ggcgtggtcg taaagcgatt cacagatgtc tgcctgttca tccgcgtcca gttcgttgag
     6661 cttctccagc agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt
     6721 ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat
     6781 accgatgaaa cgcgagagga tgctcacgat acgggttact gatgatgaac atgcccggtt
     6841 actggaacgc tgtgagggta aacagctggc ggtatggatg cggcgggtct gcctgggtga
     6901 gccggttgcc cgttccggga ggctgccgac actggcaccg ccgttactgc gtcagctggc
     6961 cgccatcggg aataacctga atcaggcagc gaggaaggtg aacagcgggc agtggtcttc
     7021 cggtgaccgg gttcaggtgg tggccgcact gatggccatc ggggatgagc tgcgccggct
     7081 gcgtctggcc gtcagggaac aggggacgcg ggatgatagt taagtttcat gccaggggaa
     7141 aaggcggtgg cagtggtccg gttgattacc tgctgggaag ggagcgtaac cgggaagggg
     7201 caacggtgct tcagggtaat ccggaagaag tccgggaact catcgatgcc acgccatttg
     7261 cgaagaaata cacgtccggt gttctctcgt tcgcggagaa ggagctgccg ccgggagggc
     7321 gtgaaaaagt gatggcgagc tttgagcgtg tactgatgcc cggtctcgaa aaaaatcagt
     7381 acagcattct gtgggtggag caccaggaca aggggcggct tgagctgaat tttgtcattc
     7441 cgaacatgga gctacagacc ggaaaacgcc tccagccgta ctacgaccgc gcagacaggc
     7501 cgagaattga tgcctggcag acgctggtga atcaccatta cgggctgcat gacccgaacg
     7561 ccccggagaa ccgccggacg ctgactctcc ctgataacct gccggaaacg aaacaggcgc
     7621 ttgctgagag cattacacgg tggatagacg ctctttatca tgtcggggag ataaaaggcc
     7681 gtcaggatgt gattcaggcg ctcacagagg cggggcttga agtggtcagg gtgacgcgaa
     7741 gcagtatcag cattgcagac ccgaacggcg ggaagaatat cagactgaaa ggagcattct
     7801 atgagcaatc ttttacagac gggcgcggag ttcgagaaaa agctgaaaga gagagccgaa
     7861 tctacagaga aaatgctgag cgacgagttc aggaagctcg aagaatctgt aagcaaggct
     7921 gtgacatcaa acgagacgaa aatcaaagac gctatagccc tgttcacagc ttcgacagag
     7981 gaatcactga aaaaacaccg ggaaggggtg aaagaggcga tgatgcagca caggaaggac
     8041 gtgttaaagc tggcagggaa tacgggcatg atgttactgg ggatggtcat tttcctgttt
     8101 accgtgagtg gcgggacgct ctggtatctt ggcgggatga tacaggcgaa cctggaagaa
     8161 atcaggaagc agaacgagag tctggagaag ctgaacgcga agacatgggg cgtgcgtta
//
