LOCUS       CP100047                9532 bp    DNA     circular BCT 19-SEP-2023
DEFINITION  Shigella flexneri strain 2457T plasmid p2457T_9, complete sequence.
ACCESSION   CP100047
VERSION     CP100047.1
DBLINK      BioProject: PRJNA851422
            BioSample: SAMN29224628
KEYWORDS    .
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9532)
  AUTHORS   Gabor,C., DeLaine,B., Hazen,T.H., Vyas,G., Mehta,A., Barry,E. and
            Rasko,D.A.
  TITLE     Complete genomes of Shigella flexneri archetype strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9532)
  AUTHORS   Hazen,T.H., Vyas,G., Mehta,A., Barry,E. and Rasko,D.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUN-2022) Institute for Genome Sciences, University of
            Maryland, School of Medicine, 670 W. Baltimore St., Baltimore, MD
            21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 11.3x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/29/2022 23:13:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,143
            CDSs (total)                      :: 5,010
            Genes (coding)                    :: 3,991
            CDSs (with protein)               :: 3,991
            Genes (RNA)                       :: 133
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 103
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 1,019
            CDSs (without protein)            :: 1,019
            Pseudo Genes (ambiguous residues) :: 0 of 1,019
            Pseudo Genes (frameshifted)       :: 569 of 1,019
            Pseudo Genes (incomplete)         :: 435 of 1,019
            Pseudo Genes (internal stop)      :: 177 of 1,019
            Pseudo Genes (multiple problems)  :: 151 of 1,019
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /strain="2457T"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /plasmid="p2457T_9"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.67 N 139.65 E"
                     /collection_date="1954"
     gene            complement(join(8544..9532,1..120))
                     /gene="wzz(fepE)"
                     /locus_tag="NKG97_25630"
                     /pseudo=""
     CDS             complement(join(8544..9532,1..120))
                     /gene="wzz(fepE)"
                     /locus_tag="NKG97_25630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /name="wzz(fepE)"
     gene            1000..1164
                     /locus_tag="NKG97_25635"
     CDS             1000..1164
                     /locus_tag="NKG97_25635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WNE54478.1"
                     /translation="MARCIKSQSLTLLEKLDALDADEQAAMCKRLHELAEELQNSIQIR
                     FEAESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2189..3298)
                     /gene="wzz(fepE)"
                     /locus_tag="NKG97_25640"
     CDS             complement(2189..3298)
                     /gene="wzz(fepE)"
                     /locus_tag="NKG97_25640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0016020 - membrane [Evidence IEA]; GO_process:
                     GO:0009103 - lipopolysaccharide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="WNE54479.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVIL
                     FFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFALF
                     IKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKNDKS
                     SLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEILALD
                     EIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIATKLNI
                     KKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPSNAIIVI
                     LSSVIGGMLACGYILLRHAMEARKMSVIE"
                     /name="wzz(fepE)"
     gene            4178..4342
                     /locus_tag="NKG97_25645"
     CDS             4178..4342
                     /locus_tag="NKG97_25645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WNE54480.1"
                     /translation="MARCIKSQSLTLLEKLDALDADEQAAMCKRLHELAEELQNSIQIR
                     FEAESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5366..6475)
                     /gene="wzz(fepE)"
                     /locus_tag="NKG97_25650"
     CDS             complement(5366..6475)
                     /gene="wzz(fepE)"
                     /locus_tag="NKG97_25650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0016020 - membrane [Evidence IEA]; GO_process:
                     GO:0009103 - lipopolysaccharide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="WNE54476.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVIL
                     FFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFALF
                     IKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKNDKS
                     SLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEILALD
                     EIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIATKLNI
                     KKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPSNAIIVI
                     LSSVIGGMLACGYILLRHAMEARKMSVIE"
                     /name="wzz(fepE)"
     gene            7355..7519
                     /locus_tag="NKG97_25655"
     CDS             7355..7519
                     /locus_tag="NKG97_25655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WNE54477.1"
                     /translation="MARCIKSQSLTLLEKLDALDADEQAAMCKRLHELAEELQNSIQIR
                     FEAESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(452..709)
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.385"
                     /coverage="89.0"
                     /evalue="3.4e-112"
     Typing          complement(834..932)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.0"
                     /coverage="100.0"
                     /evalue="1.35e-41"
     Typing          complement(1245..1331)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.43e-38"
     Typing          complement(3630..3887)
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.385"
                     /coverage="89.0"
                     /evalue="3.4e-112"
     Typing          complement(4012..4110)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.0"
                     /coverage="100.0"
                     /evalue="1.35e-41"
     Typing          complement(4423..4509)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.43e-38"
     Typing          complement(6807..7064)
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.385"
                     /coverage="89.0"
                     /evalue="3.4e-112"
     Typing          complement(7189..7287)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.0"
                     /coverage="100.0"
                     /evalue="1.35e-41"
     Typing          complement(7600..7686)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.43e-38"
     Typing          1356..1882
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.956"
                     /coverage="98.0"
                     /evalue="2.2599999999999997e-176"
     Typing          4534..5060
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.956"
                     /coverage="98.0"
                     /evalue="2.2599999999999997e-176"
     Typing          7711..8237
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.956"
                     /coverage="98.0"
                     /evalue="2.2599999999999997e-176"
ORIGIN
        1 caatttttat ttttgctgcc cataaaacgc taatcaagcc aaaaaggtca tttcttcatt
       61 accattcggc aacatgttgt aattagaaaa atgttcgtca gagacctttg agattttcat
      121 tgaaaaaaac acaatatatt aacatatggt ttttttattc tatcatatgt taggaaaaaa
      181 cctaaaaacc attcagcaga catttaacat aacattttat tgcgcgcacc gctaaaacgc
      241 gctgaacgcg attctgccgc aaacaccgca aaaagagatg ttttccccgg ggtaaaccgg
      301 aaaaatgcgt caggatcgct ttcagctcac tgcatagcta tgcatgaaag tgaatgacga
      361 tcggtttggg cgcatacagt gcccatgaga cacattttcg gacattttca gttcgtgaat
      421 ccctgactca ttgctacata tctgaacaga cacccctagc agcgccccca ggggcgtcct
      481 gtaaaaaaac acaccgcgcc gctagcagca cccctaatat aagataatgt tttttttatg
      541 aaaatagtca gtaccacccc taaaaagcgg tgttggcgca gctacaacac cgcgccgaca
      601 ccgctttttt aaatatcaca aagagagcaa gaagaactaa tttgtcataa ctctctattt
      661 ataaagaaaa atcagcaaaa acttgttttt gcgtggggtg tgggcatttc aggcatgaga
      721 acagactcgc gtttttccgg agtatcagcg gaattattga ttaagaaaca ctcgacgcag
      781 agcgaaattt tatgcaacag cggctgcact gcacagggat tatttatcag ttgatatgct
      841 gtgccatgat acgtcctaaa gaaacaggac aggatgtgca attgtaatac cgtcgcccgc
      901 aacgtcttac aattaccacc tgctcagggt ccccccccga cacccggaac tgacggagaa
      961 caacggaagg tgaacaaata acaacaaact gcgctgaata tggcgcgatg tatcaaaagc
     1021 cagagcctga cactgcttga aaaactggat gctctggatg ccgacgaaca ggcggccatg
     1081 tgcaaacgac tgcacgaact cgcagaagaa ctccagaaca gcatccagat tcgctttgag
     1141 gccgaaagtg aaacaggaac ataacgaagc tgacggaggc ggtcacggct ttcccgtggg
     1201 cgttttcgtc agcagacaag cccgtcaggg cgcgtcaggg ggttttagcg ggtgtcgggg
     1261 cgcagccatg acccagtcac gtagcgataa cggagtgtat actggcttaa ttatgaggcg
     1321 gcagagcaga tggtgagaga acagcgacat atgaggtgtg aaatgctgca cagatacgta
     1381 aggagaaaat gcagcttccg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     1441 gtcgttcggc tgcggcgagc ggtgtctgct cacgcaaaga cggatatacg gttatccaca
     1501 gaatcagggg ataacaccag aaaaaacatg tgagcaaaaa agaggccccg aaaaaaggcc
     1561 gcgccggtgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     1621 tcaagtcaga ggtggcgaaa cccgacagga cttaaagata ccaggcgttt ccccctggtg
     1681 gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcatt ccgctgttat
     1741 ggccgcgttc gtctcattcc acgcctgaca ctcagttccg ggtaggcagt tcgctccaag
     1801 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     1861 cgtcttgagt ccaacccggt aagacacgac ttaacgccat tggcagcggc cactggtaaa
     1921 caggatgtgc gagagatgta ttgagagttc ttgaagtggc agccttactg aggctacact
     1981 agaaggacag atttggtgac atgtctcgca caagactgtt accacggttc agaagttccc
     2041 caacttactg aaccttcgaa aaaccacctc cccaggtggt tttttcgttt tctagagtag
     2101 aagactacgg ctgtaaggga atgatctcaa gaagatcctt tgaacttttt ataaaagttg
     2161 cttataaaat gatgcatcag cacaactttt actcaatcac actcatcttt ctggcctcca
     2221 tggcatgacg taaaagtata tagccacatg caagcatccc tccaatgaca gaagaaagaa
     2281 ttacaattat ggcattactt ggcccatcct ttctgaccgg taacgttggg ctcaattgat
     2341 actgaaatgg cataaaatca acatccccca ctttcgccac aaccaattga ttcacaacat
     2401 attgacgatt tcgcaactcc ccactcaatt ccgaaacatc cttgattgat tttttgatgt
     2461 tcaatttagt tgctatacca tcagaaccga gagccacagg aaaatctggg tcatccttca
     2521 taatctgacc attgctgtat acaggttttt ttattccagc cgcattagca acctccagtg
     2581 aataattgag gcgtcgaata tctgcattca gctggtttct aatttttatc tcatccagtg
     2641 caagaatttc tttttcaaca ttagttttaa cttctagttt atttcttata tcttccatca
     2701 aatcccttac aacaagggaa gaaacataat taatataccc agacaacaca tcgtgagctt
     2761 ctttacttgt tggagcagta aaactcagag tccatgatac atacaatgat gacttgtcgt
     2821 ttttcttact ggcattatca tcaatcgact tcatattttt actcaactca acaattgcac
     2881 gatgcagttc gagagcgcta atgttattct ttttaataat ctccataaca taaggagatg
     2941 atctcatata ctcattcaac gaattaacgg actgaaattt cttaataaac agagcaaatg
     3001 cgctatcacg attgatatca aaatccactc ccagaacatg aagcttggaa agctccttat
     3061 ttaattcact ccattgaaca gactcagcag gagtaaccac agcagaacta gtccattttt
     3121 gtggcaaaac aaaagaaaca agaaagccaa tacaagcaaa aaatagaatc accccaacaa
     3181 tttttatttt tgctgcccat aaaacgctaa tcaagccaaa aaggtcaatt tcttcattac
     3241 cattcggcaa catgttgtaa ttagaaaaat gttcgtcaga gacctttgag attttcattg
     3301 aaaaaaacac aatatattaa catatggttt ttttattcta tcatatgtta ggaaaaaacc
     3361 taaaaaccat tcagcagaca tttaacataa cattttattg cgcgcaccgc taaaacgcgc
     3421 tgaacgcgat tctgccgcaa acaccgcaaa aagagatgtt ttccccgggg taaaccggaa
     3481 aaatgcgtca ggatcgcttt cagctcactg catagctatg catgaaagtg aatgacgatc
     3541 ggtttgggcg catacagtgc ccatgagaca cattttcgga cattttcagt tcgtgaatcc
     3601 ctgactcatt gctacatatc tgaacagaca cccctagcag cgcccccagg ggcgtcctgt
     3661 aaaaaaacac accgcgccgc tagcagcacc cctaatataa gataatgttt tttttatgaa
     3721 aatagtcagt accaccccta aaaagcggtg ttggcgcagc tacaacaccg cgccgacacc
     3781 gcttttttaa atatcacaaa gagagcaaga agaactaatt tgtcataact ctctatttat
     3841 aaagaaaaat cagcaaaaac ttgtttttgc gtggggtgtg ggcatttcag gcatgagaac
     3901 agactcgcgt ttttccggag tatcagcgga attattgatt aagaaacact cgacgcagag
     3961 cgaaatttta tgcaacagcg gctgcactgc acagggatta tttatcagtt gatatgctgt
     4021 gccatgatac gtcctaaaga aacaggacag gatgtgcaat tgtaataccg tcgcccgcaa
     4081 cgtcttacaa ttaccacctg ctcagggtcc ccccccgaca cccggaactg acggagaaca
     4141 acggaaggtg aacaaataac aacaaactgc gctgaatatg gcgcgatgta tcaaaagcca
     4201 gagcctgaca ctgcttgaaa aactggatgc tctggatgcc gacgaacagg cggccatgtg
     4261 caaacgactg cacgaactcg cagaagaact ccagaacagc atccagattc gctttgaggc
     4321 cgaaagtgaa acaggaacat aacgaagctg acggaggcgg tcacggcttt cccgtgggcg
     4381 ttttcgtcag cagacaagcc cgtcagggcg cgtcaggggg ttttagcggg tgtcggggcg
     4441 cagccatgac ccagtcacgt agcgataacg gagtgtatac tggcttaatt atgaggcggc
     4501 agagcagatg gtgagagaac agcgacatat gaggtgtgaa atgctgcaca gatacgtaag
     4561 gagaaaatgc agcttccggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     4621 cgttcggctg cggcgagcgg tgtctgctca cgcaaagacg gatatacggt tatccacaga
     4681 atcaggggat aacaccagaa aaaacatgtg agcaaaaaag aggccccgaa aaaaggccgc
     4741 gccggtggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     4801 aagtcagagg tggcgaaacc cgacaggact taaagatacc aggcgtttcc ccctggtggc
     4861 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
     4921 ccgcgttcgt ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct
     4981 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     5041 tcttgagtcc aacccggtaa gacacgactt aacgccattg gcagcggcca ctggtaaaca
     5101 ggatgtgcga gagatgtatt gagagttctt gaagtggcag ccttactgag gctacactag
     5161 aaggacagat ttggtgacat gtctcgcaca agactgttac cacggttcag aagttcccaa
     5221 cttactgaac cttcgaaaaa ccacctcccc aggtggtttt ttcgttttct agagtagaag
     5281 actacggctg taagggaatg atctcaagaa gatcctttga actttttata aaagttgctt
     5341 ataaaatgat gcatcagcac aacttttact caatcacact catctttctg gcctccatgg
     5401 catgacgtaa aagtatatag ccacatgcaa gcatccctcc aatgacagaa gaaagaatta
     5461 caattatggc attacttggc ccatcctttc tgaccggtaa cgttgggctc aattgatact
     5521 gaaatggcat aaaatcaaca tcccccactt tcgccacaac caattgattc acaacatatt
     5581 gacgatttcg caactcccca ctcaattccg aaacatcctt gattgatttt ttgatgttca
     5641 atttagttgc tataccatca gaaccgagag ccacaggaaa atctgggtca tccttcataa
     5701 tctgaccatt gctgtataca ggttttttta ttccagccgc attagcaacc tccagtgaat
     5761 aattgaggcg tcgaatatct gcattcagct ggtttctaat ttttatctca tccagtgcaa
     5821 gaatttcttt ttcaacatta gttttaactt ctagtttatt tcttatatct tccatcaaat
     5881 cccttacaac aagggaagaa acataattaa tatacccaga caacacatcg tgagcttctt
     5941 tacttgttgg agcagtaaaa ctcagagtcc atgatacata caatgatgac ttgtcgtttt
     6001 tcttactggc attatcatca atcgacttca tatttttact caactcaaca attgcacgat
     6061 gcagttcgag agcgctaatg ttattctttt taataatctc cataacataa ggagatgatc
     6121 tcatatactc attcaacgaa ttaacggact gaaatttctt aataaacaga gcaaatgcgc
     6181 tatcacgatt gatatcaaaa tccactccca gaacatgaag cttggaaagc tccttattta
     6241 attcactcca ttgaacagac tcagcaggag taaccacagc agaactagtc catttttgtg
     6301 gcaaaacaaa agaaacaaga aagccaatac aagcaaaaaa tagaatcacc ccaacaattt
     6361 ttatttttgc tgcccataaa acgctaatca agccaaaaag gtcaatttct tcattaccat
     6421 tcggcaacat gttgtaatta gaaaaatgtt cgtcagagac ctttgagatt ttcattgaaa
     6481 aaaacacaat atattaacat atggtttttt tattctatca tatgttagga aaaaacctaa
     6541 aaaccattca gcagacattt aacataacat tttattgcgc gcaccgctaa aacgcgctga
     6601 acgcgattct gccgcaaaca ccgcaaaaag agatgttttc cccggggtaa accggaaaaa
     6661 tgcgtcagga tcgctttcag ctcactgcat agctatgcat gaaagtgaat gacgatcggt
     6721 ttgggcgcat acagtgccca tgagacacat tttcggacat tttcagttcg tgaatccctg
     6781 actcattgct acatatctga acagacaccc ctagcagcgc ccccaggggc gtcctgtaaa
     6841 aaaacacacc gcgccgctag cagcacccct aatataagat aatgtttttt ttatgaaaat
     6901 agtcagtacc acccctaaaa agcggtgttg gcgcagctac aacaccgcgc cgacaccgct
     6961 tttttaaata tcacaaagag agcaagaaga actaatttgt cataactctc tatttataaa
     7021 gaaaaatcag caaaaacttg tttttgcgtg gggtgtgggc atttcaggca tgagaacaga
     7081 ctcgcgtttt tccggagtat cagcggaatt attgattaag aaacactcga cgcagagcga
     7141 aattttatgc aacagcggct gcactgcaca gggattattt atcagttgat atgctgtgcc
     7201 atgatacgtc ctaaagaaac aggacaggat gtgcaattgt aataccgtcg cccgcaacgt
     7261 cttacaatta ccacctgctc agggtccccc cccgacaccc ggaactgacg gagaacaacg
     7321 gaaggtgaac aaataacaac aaactgcgct gaatatggcg cgatgtatca aaagccagag
     7381 cctgacactg cttgaaaaac tggatgctct ggatgccgac gaacaggcgg ccatgtgcaa
     7441 acgactgcac gaactcgcag aagaactcca gaacagcatc cagattcgct ttgaggccga
     7501 aagtgaaaca ggaacataac gaagctgacg gaggcggtca cggctttccc gtgggcgttt
     7561 tcgtcagcag acaagcccgt cagggcgcgt cagggggttt tagcgggtgt cggggcgcag
     7621 ccatgaccca gtcacgtagc gataacggag tgtatactgg cttaattatg aggcggcaga
     7681 gcagatggtg agagaacagc gacatatgag gtgtgaaatg ctgcacagat acgtaaggag
     7741 aaaatgcagc ttccggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     7801 tcggctgcgg cgagcggtgt ctgctcacgc aaagacggat atacggttat ccacagaatc
     7861 aggggataac accagaaaaa acatgtgagc aaaaaagagg ccccgaaaaa aggccgcgcc
     7921 ggtggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     7981 tcagaggtgg cgaaacccga caggacttaa agataccagg cgtttccccc tggtggctcc
     8041 ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg tcattccgct gttatggccg
     8101 cgttcgtctc attccacgcc tgacactcag ttccgggtag gcagttcgct ccaagctggg
     8161 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     8221 tgagtccaac ccggtaagac acgacttaac gccattggca gcggccactg gtaaacagga
     8281 tgtgcgagag atgtattgag agttcttgaa gtggcagcct tactgaggct acactagaag
     8341 gacagatttg gtgacatgtc tcgcacaaga ctgttaccac ggttcagaag ttccccaact
     8401 tactgaacct tcgaaaaacc acctccccag gtggtttttt cgttttctag agtagaagac
     8461 tacggctgta agggaatgat ctcaagaaga tcctttgaac tttttataaa agttgcttat
     8521 aaaatgatgc atcagcacaa cttttactca atcacactca tctttctggc ctccatggca
     8581 tgacgtaaaa gtatatagcc acatgcaagc atccctccaa tgacagaaga aagaattaca
     8641 attatggcat tacttggccc atcctttctg accggtaacg ttgggctcaa ttgatactga
     8701 aatggcataa aatcaacatc ccccactttc gccacaacca attgattcac aacatattga
     8761 cgatttcgca actccccact caattccgaa acatccttga ttgatttttt gatgttcaat
     8821 ttagttgcta taccatcaga accgagagcc acaggaaaat ctgggtcatc cttcataatc
     8881 tgaccattgc tgtatacagg tttttttatt ccagccgcat tagcaacctc cagtgaataa
     8941 ttgaggcgtc gaatatctgc attcagctgg tttctaattt ttatctcatc cagtgcaaga
     9001 atttcttttt caacattagt tttaacttct agtttatttc ttatatcttc catcaaatcc
     9061 cttacaacaa gggaagaaac ataattaata tacccagaca acacatcgtg agcttcttta
     9121 cttgttggag cagtaaaact cagagtccat gatacataca atgatgactt gtcgtttttc
     9181 ttactggcat tatcatcaat cgacttcata tttttactca actcaacaat tgcacgatgc
     9241 agttcgagag cgctaatgtt attcttttta ataatctcca taacataagg agatgatctc
     9301 atatactcat tcaacgaatt aacggactga aatttcttaa taaacagagc aaatgcgcta
     9361 tcacgattga tatcaaaatc cactcccaga acatgaagct tggaaagctc cttatttaat
     9421 tcactccatt gaacagactc agcaggagta accacagcag aactagtcca tttttgtggc
     9481 aaaacaaaag aaacaagaaa gccaatacaa gcaaaaaata gaatcacccc aa
//
