LOCUS       CP100039                1901 bp    DNA     circular BCT 21-JUL-2022
DEFINITION  Escherichia coli strain EC204A1 plasmid p204A1-1K, complete
            sequence.
ACCESSION   CP100039
VERSION     CP100039.1
DBLINK      BioProject: PRJNA827955
            BioSample: SAMN27625699
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Tang,B., Chang,J., Yang,H., Wu,J. and Li,J.
  TITLE     A 12-year investigation on the antimicrobial resistance of
            Escherichia coli from diseased swine
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Tang,B., Chang,J., Wu,J., Yang,H., Lin,J., Guan,C., Jiang,H.,
            Chen,H. and Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
REFERENCE   3  (bases 1 to 1901)
  AUTHORS   Tang,B., Chang,J., Yang,H., Wu,J. and Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. JAN-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/29/2022 22:18:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,744
            CDSs (total)                      :: 5,616
            Genes (coding)                    :: 5,416
            CDSs (with protein)               :: 5,416
            Genes (RNA)                       :: 128
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 200
            CDSs (without protein)            :: 200
            Pseudo Genes (ambiguous residues) :: 0 of 200
            Pseudo Genes (frameshifted)       :: 72 of 200
            Pseudo Genes (incomplete)         :: 131 of 200
            Pseudo Genes (internal stop)      :: 33 of 200
            Pseudo Genes (multiple problems)  :: 35 of 200
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC204A1"
                     /isolation_source="gut"
                     /db_xref="taxon:562"
                     /plasmid="p204A1-1K"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2013-03"
     gene            complement(join(1802..1901,1..26))
                     /locus_tag="MZH44_28710"
     CDS             complement(join(1802..1901,1..26))
                     /locus_tag="MZH44_28710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="UTU32775.1"
                     /translation="MWLPVLFRFLVDSLATLGHRLAASGITSRMLSVIPLLFFQG"
                     /name="replication protein"
     gene            520..1068
                     /locus_tag="MZH44_28715"
     CDS             520..1068
                     /locus_tag="MZH44_28715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="firmicute plasmid replication protein RepL"
                     /protein_id="UTU32776.1"
                     /translation="MLEQQFEDFEQQKRRDHQVQDQSKNAKQKRDRLNPPDWSQVTKAG
                     WNMLDAISEKVPRAFRLYSFFAQNIDKSCGAVVCDQKFLADYFGVSLRTIQYWIGDLES
                     IGAIVKIPVGIYYAYALDPYQVWRGYNNTKKYAAFNAKTLTQKDGVIDRKLRLMLSRRE
                     EFESSLKYSNHDSEQGDAL"
                     /name="firmicute plasmid replication protein RepL"
     gene            1065..1352
                     /locus_tag="MZH44_28720"
     CDS             1065..1352
                     /locus_tag="MZH44_28720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UTU32774.1"
                     /translation="MRIKYESRWRLEELKLEICSSIDELINKYGIEYATGINLYMNCED
                     KEFNLVDVINEDGNKIMFVVSNDEKIQKPKKIAKKKTPAPVIELDKYRKK"
                     /name="hypothetical protein"
     Typing          complement(1589..1849)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.237"
                     /coverage="100.0"
                     /evalue="3.82e-131"
ORIGIN
        1 aagcggaaga gaaccggaag ccacattgag cacttacgca ctgatgcggg gtgtcggggt
       61 gaagccctga ccaggtggca tttgtctgat tgcgtgtgcg cggtccgaca aatgcacatc
      121 ctgtcccgat ttctgaggct ttttagcggt gaacggacgg caaaaccatg atggagttgt
      181 tgaacgttga gagcatgaaa tctggcaaaa atcacggctg gtaaggcttg cagaggagta
      241 agccagtata cactccgcta tcgctacgtg actgggtcag ggctgcgccc cgacacccca
      301 aaagcagtta ggtagtgtta aatcatgagc agtaaaaaaa cgctttgata gatctttaga
      361 gcaaaaattg acatttcttt aaaaatcatt gacttaagac accatcatac gaaacgttat
      421 ttgcatgtgt atacgcaaac atgtttgcat gtgttgtttc gtactgtacg atataaattt
      481 cgtaaggtgc gaaatatcat tgcaggtaag gatgcaagta tgttggaaca gcaatttgaa
      541 gactttgaac agcaaaaacg gcgagatcat caggtacaag atcaatccaa gaacgctaag
      601 caaaaacgag atcggttgaa tcctccagac tggtcgcagg ttactaaggc tggttggaat
      661 atgttggacg ctatatcaga aaaagttcct agagcatttc ggttatattc tttttttgcc
      721 cagaatatag ataaatcatg tggggctgtt gtatgtgatc aaaaattctt agctgattat
      781 ttcggtgtta gtttaagaac gatccaatat tggattgggg atttagaaag tattggcgca
      841 atagttaaaa taccagtagg catatattat gcctatgcat tagatcctta tcaggtatgg
      901 cgtggatata acaatactaa aaaatatgca gcatttaacg caaaaacatt aactcaaaaa
      961 gatggggtta tagatcgtaa attgcggctt atgctttcta ggcgtgaaga gtttgaaagt
     1021 tccttaaagt actctaatca tgactcagag cagggtgatg cactatgaga attaaatatg
     1081 aatcacgttg gagattagaa gaactaaaat tagaaatatg cagttcaata gatgagttaa
     1141 ttaataaata tggtattgaa tatgcaacag gaataaatct ttatatgaat tgtgaagata
     1201 aagaatttaa tcttgttgat gttatcaatg aagatggaaa taaaataatg tttgtcgtta
     1261 gcaacgatga aaaaatacaa aagccaaaaa aaatagcaaa gaaaaaaact ccagcacctg
     1321 taattgagct agataaatat cgaaaaaaat gaccattgag ccagcggcag gcggacaatc
     1381 aggggctacg tgttaacgtt ctgaccatga ttgtctatcc tgcattgctc ttttgccgcc
     1441 tcgaatccct tacgtgtttt tgttccccgc tctccagaac gaccgagccg ccacggttcc
     1501 gatagcgcct tgagcgcaac cggaaaccgt ctggggagaa ccccagaccc ccatcgctgt
     1561 aacaccaatg gtctacgacc acctaccaac cgaatagctg cctgtgccgt gcaaataggc
     1621 tttgcagggg gctgacgctg ttcctagcga cctgaaagcc cctggttagg ggtatcggcg
     1681 cgcctgacct cctgaaacgc tctgtaagcg tttttcgcgc ctcccatgcc ctgacgggca
     1741 tttaagaccc gtttagtgcg tctgagagct tctgagcgcg tttgaggggc tatctgacga
     1801 ctcatccctg aaagaacaac aacgggatca ccgacaacat gcgagaggtg ataccgctcg
     1861 ccgcaagtcg atgaccgagc gtagcgagcg aatcgacgag g
//
