LOCUS       CP100038                2009 bp    DNA     circular BCT 21-JUL-2022
DEFINITION  Escherichia coli strain EC204A1 plasmid p204A1-2K, complete
            sequence.
ACCESSION   CP100038
VERSION     CP100038.1
DBLINK      BioProject: PRJNA827955
            BioSample: SAMN27625699
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Tang,B., Chang,J., Yang,H., Wu,J. and Li,J.
  TITLE     A 12-year investigation on the antimicrobial resistance of
            Escherichia coli from diseased swine
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Tang,B., Chang,J., Wu,J., Yang,H., Lin,J., Guan,C., Jiang,H.,
            Chen,H. and Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
REFERENCE   3  (bases 1 to 2009)
  AUTHORS   Tang,B., Chang,J., Yang,H., Wu,J. and Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. JAN-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/29/2022 22:18:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,744
            CDSs (total)                      :: 5,616
            Genes (coding)                    :: 5,416
            CDSs (with protein)               :: 5,416
            Genes (RNA)                       :: 128
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 200
            CDSs (without protein)            :: 200
            Pseudo Genes (ambiguous residues) :: 0 of 200
            Pseudo Genes (frameshifted)       :: 72 of 200
            Pseudo Genes (incomplete)         :: 131 of 200
            Pseudo Genes (internal stop)      :: 33 of 200
            Pseudo Genes (multiple problems)  :: 35 of 200
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC204A1"
                     /isolation_source="gut"
                     /db_xref="taxon:562"
                     /plasmid="p204A1-2K"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2013-03"
     gene            complement(136..465)
                     /locus_tag="MZH44_28700"
     CDS             complement(136..465)
                     /locus_tag="MZH44_28700"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UTU32772.1"
                     /translation="MVSFIAQGCAPNPANASRYQIKNLSAFTVPEFVKQKIAGSRAFGV
                     GSDFVIDDGTGKKHWGWWLVSRSEANHLQDGFVVHKISGKACECFVGKTLSGLLFLPPE
                     NQLRF"
                     /name="hypothetical protein"
     gene            complement(875..1861)
                     /locus_tag="MZH44_28705"
     CDS             complement(875..1861)
                     /locus_tag="MZH44_28705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="UTU32773.1"
                     /translation="MENPELQNLTDYSPSDAPWDAHRSASDDVGGIYLLAAEYERYGAR
                     MASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDARWMFLTLTVRNCAIGELGETLSRMNMAFQRLKDRKEFRPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMVPPSMFTRDYVKHARWVELWRECLRVDYDPSVDVRAVKPRKPKDGE
                     SLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGALKDVLKL
                     DQETDADMVIGDDISEGDDDGSRIAFEWKTEVRKYRRSPTKDKTESG"
                     /name="protein rep"
     Typing          complement(792..873)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.181"
                     /coverage="90.0"
                     /evalue="7.3e-29"
     Typing          958..1149
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.964"
                     /coverage="100.0"
                     /evalue="6.14e-93"
ORIGIN
        1 ctttcgacag aatacttcta aaaccttcta caacgcaaat aagccgcaaa gccttgccac
       61 acaaggcctg tagctatttt ttacccccag agttatccct taagtataaa tacaaggatc
      121 gcgcgcgaga tttgctcaaa atctgagctg attttctggc ggcaaaaaca acaaccccga
      181 aagcgttttc ccgacaaaac actcacaggc ttttccactg attttgtgaa caacaaaacc
      241 gtcttgcagg tggttggcct cactgcgtga caccaaccac catccccagt gtttttttcc
      301 cgtgccgtcg tcaatcacaa aatcggaacc cacgccaaag gcgcgggacc ccgcgatttt
      361 ctgtttgacg aattcgggaa cggtgaacgc tgacagattt ttaatctgat agcgtgaggc
      421 gttggcgggg ttcggggcgc agccctgagc gatgaacgaa accaatgcag gatagacaaa
      481 ccgatgtcaa ttgttaccaa ttttcaaatt gggcatttgt catcggatta tcaacctgtg
      541 cctgccgcag gctcttttca aaaatcaaaa acgcgtgttt ttaaaaacac actctttcca
      601 aaatccagaa ttttaatttt tgttttcacc gaaaattaaa aacagagcct gcggcaggcg
      661 gacaatctgg gcaacgtgtt aacgttctga ccatggttgt ctatcctgca ttggtttcgt
      721 ttgtcctgca ttggtttcgt ttgtcctgcg gcctgctacg ctcaaaaaac cgagatacgg
      781 cgagaatttt cgggacagga tgtgcaattg taataccgtc acacgcgacg ctatcacaat
      841 tgccatctgg tcaggggtgc accccgacac cccgctagcc tgattcagtt ttatctttcg
      901 tcggtgagcg acggtacttt ctgacctcgg ttttccactc gaacgcgatg cgtgaaccgt
      961 catcgtcgcc ctcggaaata tcatccccga tcaccatgtc ggcgtcggtt tcctgatcga
     1021 gtttcagcac gtctttgagt gcgccgccgg tcgccacaaa gcggcgtttg tgcgtctggc
     1081 gagtcagttc gaggaaccat tcaggatcgg ccaccatgtc agccggcttc gtggaatatt
     1141 tcagggtttc ggccaccgca ccgcgcacca gttcggcggt cgcgcaggcc agcgattcgc
     1201 cgtccttcgg cttgcggggt ttcactgccc ggacatcaac actcgggtcg taatctaccc
     1261 gcagacactc ccgccacaat tcaacccagc gcgcatgttt gacatagtca cgcgtgaaca
     1321 tcgagggcgg caccatcatc agggtatgga agtgcgggtg tgcgctaccg tcgctgcctc
     1381 gagtaacctc cgtcgtgcgg atccagccct gaacaggtcg aaactctttg cgatccttca
     1441 gtcgctggaa ggccatgttc attcggctca gtgtctcgcc cagctcccct atcgcacagt
     1501 tgcgcacggt cagggtcaga aacatccatc gtgcgtctgg atagtcagcc acaatacgcg
     1561 gcaaagactg ataaaaacgg gcttgccaca tcagggagcg cctccactgg cagacaggac
     1621 agtgccggac acggcagaaa tgcgcctcac gcagcctcag tcgcgtttct ccggtttcct
     1681 tgagggtaga ccacccaaaa cgcagtaaac cgccacagga ggccatacgc gccccgtaac
     1741 gctcatattc agctgcaagc aggtaaatcc ctcccacgtc gtcagaagcg cttctgtgag
     1801 cgtcccatgg cgcatctgag ggggaatagt ccgtgaggtt ctggagttca ggattttcca
     1861 tgcacaccac ccgattgtgg gcgatggctc atctgacgtg ttatcattgc catatcacct
     1921 gtgttgtcgt gtcaccaatt cgaattccta caggtggtca cacccccgaa ctgttagcgc
     1981 agttgcgggg gtcgctttta atgcgacca
//
