LOCUS       CP100037                3373 bp    DNA     circular BCT 21-JUL-2022
DEFINITION  Escherichia coli strain EC204A1 plasmid p204A1-3K, complete
            sequence.
ACCESSION   CP100037
VERSION     CP100037.1
DBLINK      BioProject: PRJNA827955
            BioSample: SAMN27625699
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Tang,B., Chang,J., Yang,H., Wu,J. and Li,J.
  TITLE     A 12-year investigation on the antimicrobial resistance of
            Escherichia coli from diseased swine
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Tang,B., Chang,J., Wu,J., Yang,H., Lin,J., Guan,C., Jiang,H.,
            Chen,H. and Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
REFERENCE   3  (bases 1 to 3373)
  AUTHORS   Tang,B., Chang,J., Yang,H., Wu,J. and Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. JAN-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/29/2022 22:18:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,744
            CDSs (total)                      :: 5,616
            Genes (coding)                    :: 5,416
            CDSs (with protein)               :: 5,416
            Genes (RNA)                       :: 128
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 200
            CDSs (without protein)            :: 200
            Pseudo Genes (ambiguous residues) :: 0 of 200
            Pseudo Genes (frameshifted)       :: 72 of 200
            Pseudo Genes (incomplete)         :: 131 of 200
            Pseudo Genes (internal stop)      :: 33 of 200
            Pseudo Genes (multiple problems)  :: 35 of 200
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC204A1"
                     /isolation_source="gut"
                     /db_xref="taxon:562"
                     /plasmid="p204A1-3K"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2013-03"
     gene            complement(1126..1359)
                     /locus_tag="MZH44_28675"
     CDS             complement(1126..1359)
                     /locus_tag="MZH44_28675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UTU32770.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHEVTQRNNA
                     ILNERVVILSQQVQRLAGQVDRLSRLFIANNS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1366..1926)
                     /locus_tag="MZH44_28680"
     CDS             complement(1366..1926)
                     /locus_tag="MZH44_28680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="UTU32771.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISEHTTSVKKQLDTLSTTVSMQFSTTEAELSRQQKKLLWRVIKGRILFPALTA
                     LSVTGGIFLGGWGLMEWQESKIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGVKGENTWTVGDKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(1955..2395)
                     /locus_tag="MZH44_28685"
                     /pseudo=""
     CDS             complement(1955..2395)
                     /locus_tag="MZH44_28685"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2604..2927)
                     /locus_tag="MZH44_28690"
     CDS             complement(2604..2927)
                     /locus_tag="MZH44_28690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UTU32768.1"
                     /translation="MLTIRVTDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     APPLLRHLAAIGNNLNQTARKVNSGQWSSIDRVHVVAALMAIEGELRQLRQAVREQGVR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2991..3182
                     /locus_tag="MZH44_28695"
     CDS             2991..3182
                     /locus_tag="MZH44_28695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UTU32769.1"
                     /translation="MTKQEKTALNMARFIRNQTLTLLEKLNDLDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(3264..3344)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.585"
                     /coverage="93.0"
                     /evalue="4.04e-16"
     Typing          complement(1044..2614)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.439"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agtaaaaagc cgcacagatg cgtaaaaaca gcgcgcccga taaacaacac acacgcggac
       61 actggcacag gcgcgtaagt gcacaatgct tgcctctgtg actcttccgc ttcctcgccc
      121 actcgctcac tacgttcggt cgctggggct gcggcgagcg gtgggatcac tctcgttgtg
      181 atgcagatcc gttttttttt ttgcactgga gttatcagga gtagcggtgc acatgggatc
      241 gtgcgatatg ctggatggtg gaaggtcgga gggaacggca acaactcacc cctcccagtg
      301 aagggacggg tagtttgccg ctctcgatat gccggacgga gccaaggcca tcgatcacgg
      361 ggcgcgtcag tggaagaacc cgcgcctgcg tgacgtatgc gtcttcgccg caccatctca
      421 cccgtcacgc caccacatac cgggaactgc cgccaaggtc taactcaacc aatctagccg
      481 cgctacatac ggtttttgtg gcaactcatc aggtgtccgc tgtcgatgaa actctctgcg
      541 ccggtagcgg aactctcacg cgtgcggtcg ccgtcgtcac agctccccca accgcaaacg
      601 attctcgcaa atacgcaagt atctgcaaaa acaggttttt ttcggggttc ctgggagact
      661 gtgctgttga aaaactcttg aagaatacag aaggctcatg caaactgaat gaactggttt
      721 ctgttgtgga tctcaacagt ttccacagga acattgagga ggtcagagac ctcaatgaac
      781 ccgaaacagc tcgccttcct ggcgagctgt tttcgttcta cgcccaccga cgaaagatcg
      841 caagtgcaat aacgagattt ttcacgtctt tacgctcaaa aagtaaaaaa accatgtttt
      901 cccctggctg aatccccaaa aacgcgtgaa atgcgttttc aggatgttgc atagctatgc
      961 acaaatacgg cacacgattt gtttcgctcg ccccagccgt cgaacgggtc aaaatggcaa
     1021 gatttcgtca cttctccggt cacatctcca ttgatggccc actccgaacc cgtgccgcgc
     1081 gttcctgtac ctcatgtctg taccgggcac gctcctgttc atcccttagc tgttgttcgc
     1141 gataaataac cgactcaagc gatccacttg cccggctaat ctctgcacct gctgactcaa
     1201 tatcacgacg cgctcgttca gtatcgcgtt gttccgttgc gtaacctcgt gcatcttctg
     1261 caattccgcg aaggcgctct cccactcgtt cagccgccgc atatagtcct gttgcaaatg
     1321 ctctaaggcg ttcagcaaat gcccttccag ttccgtcata ctcgtttact ccctgaccag
     1381 cctcacggcg ttcttgtcgc ccaccgtcca ggtgttttcc cctttcaccc cgtccggcaa
     1441 caccagaaat ttcccgttct cgccattgac gaacgtcacc ccccaggttt ttgcctcgag
     1501 tttcgccagc gtgttttcct gctctctgat agtcaggata tttttcgcaa tcttgctttc
     1561 ctgccactcc atcagccccc agccccccag gaaaatcccg cccgtgacgc tgagcgcggt
     1621 cagcgccgga aacagtatcc gccctttgat cacccgccag agcagttttt tctgctgccg
     1681 ggacagttcg gcctctgtgg tgctgaactg catgctcacg gtggtgctca gcgtatccag
     1741 ttgctttttc acggatgttg tatgttcgct gatagcgtct ctgattttct gcctgtttaa
     1801 gctcaatgcc ttgtctacag actcttcgag cttcctgaac tcgctgttca gcatgttctc
     1861 ggtagaagcg gctcgctctt tcagtttctt ctcgaagtct gttcccattt gtaaaagatt
     1921 gctcatacag cgcccccttt agcctgagat tccgcccccc ctccgggtcg gcaatactga
     1981 tgctgctttt ggttgtcctc accacctcaa aacctgccgc tgtaagcgcc tctgtgacat
     2041 cctgacgcgt ttttattgct ccggcatggt aaagagcctc aagaccccgt gtaatcgctt
     2101 ctgcggcctc ctgtttcgct tttggcagat tatttggcgt gacaagcgtc cgtctgttct
     2161 ccggtgcgtt cgggtcacgc agcccgtaat gatgattaac cagcgtctgc caggcattta
     2221 tcctcggcct gtccgcacgg tcgtagtacg gctggaggcg cttaccgctc tgtagctcca
     2281 tgttcgggat gacaaaattc agttcgagcc gccccttatc ctggtgttcc acccagagaa
     2341 tgctgtactg gtctttttcg agaccgggca taagaacacg ctcaaagctc gtcatcactc
     2401 tttcgcgttc acccggtggt agctccttct ccgcgaacga cagaacgcct gacgtgtatt
     2461 tcttcgaaaa tggcgtggaa tcaatgagtt cccgcacttc ttccgggtta ccccggagca
     2521 ccgttgcgcc ttcgcggtta cgctcccgcc ccagcaggta atcgaccggg ccacttccac
     2581 cgcctttccc cctggcatga aatttaacta tcatcccgta ccccctgttc cctgacagcc
     2641 tgccgcaact ggcgcaattc accctcgatg gccatcagtg cggccaccac atgaacccgg
     2701 tcaatcgaag accactggcc gctgttcacc ttccgggctg tctggttcag gttgttcccg
     2761 atggcagcca gatgccgcag caatggcggt gccagtgtcg gcagttttcc tgtgcgtgca
     2821 accggctccc ccagacagac ccgccgcatc cattccgcca gtcgtttacc ctcgcagcgt
     2881 tccagtaacc gggcgtgctc ctcatcggta acccgtatcg tgagcatctt ctcgcgtttc
     2941 atcggtatca ttccccctaa caaatccccc atacaaggag ccttaagacc atgaccaaac
     3001 aggaaaaaac agcccttaac atggctcgct ttatcagaaa ccagacgcta accctgctgg
     3061 aaaaactcaa cgatctggac gcggacgaac aggcagacat ctgcgaatca cttcacgacc
     3121 acgccgacga actctaccgc agttgcctcg cacgcttcgg cgatgacggt gaaagcaact
     3181 gaaacatgca gctcccggag acgttcacag cttgcctgtg agcggatgcc gggagcagac
     3241 aagcccgcca gggcgcgtca gtgcgggtgt cggggcgcag ccctgaccca gttcacagag
     3301 cgatagcgat gtgtatactg gctttcctca ttttgcatcg gtgcagagtg gatgggaagt
     3361 gcaccatgtc agg
//
