LOCUS       CP100036                4532 bp    DNA     circular BCT 21-JUL-2022
DEFINITION  Escherichia coli strain EC204A1 plasmid p204A1-4K2, complete
            sequence.
ACCESSION   CP100036
VERSION     CP100036.1
DBLINK      BioProject: PRJNA827955
            BioSample: SAMN27625699
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Tang,B., Chang,J., Yang,H., Wu,J. and Li,J.
  TITLE     A 12-year investigation on the antimicrobial resistance of
            Escherichia coli from diseased swine
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4532)
  AUTHORS   Tang,B., Chang,J., Wu,J., Yang,H., Lin,J., Guan,C., Jiang,H.,
            Chen,H. and Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
REFERENCE   3  (bases 1 to 4532)
  AUTHORS   Tang,B., Chang,J., Yang,H., Wu,J. and Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. JAN-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/29/2022 22:18:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,744
            CDSs (total)                      :: 5,616
            Genes (coding)                    :: 5,416
            CDSs (with protein)               :: 5,416
            Genes (RNA)                       :: 128
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 200
            CDSs (without protein)            :: 200
            Pseudo Genes (ambiguous residues) :: 0 of 200
            Pseudo Genes (frameshifted)       :: 72 of 200
            Pseudo Genes (incomplete)         :: 131 of 200
            Pseudo Genes (internal stop)      :: 33 of 200
            Pseudo Genes (multiple problems)  :: 35 of 200
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC204A1"
                     /isolation_source="gut"
                     /db_xref="taxon:562"
                     /plasmid="p204A1-4K2"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2013-03"
     gene            491..814
                     /locus_tag="MZH44_28645"
     CDS             491..814
                     /locus_tag="MZH44_28645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UTU32767.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1023..1457
                     /locus_tag="MZH44_28650"
                     /pseudo=""
     CDS             1023..1457
                     /locus_tag="MZH44_28650"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1492..1983
                     /locus_tag="MZH44_28655"
     CDS             1492..1983
                     /locus_tag="MZH44_28655"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="UTU32765.1"
                     /translation="MSNLFTASRDFEEKLKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAISAHEQNMSEAMEKNRRSVLLTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            1990..2205
                     /locus_tag="MZH44_28660"
     CDS             1990..2205
                     /locus_tag="MZH44_28660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UTU32766.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     MLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3146..4051)
                     /locus_tag="MZH44_28665"
     CDS             complement(3146..4051)
                     /locus_tag="MZH44_28665"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UTU32763.1"
                     /translation="MWKGCWLTTMPSTGTTEQKNDKTCILGLFLQTHNRIKYFFYLIGL
                     SNEIHHIGKNFHHCSFYFIPLFNLCFNKDYWCMKMQHNSSDIDLIELFLFLKTKIASII
                     LFVIFSLILSFTFLFITKDKVIIKYQLNMINNSPSMIINCGDDFYCKANAIQSIINNKS
                     KSITSNIDEKAQKINLSWSGDVRDKPVLIAEVNTIHRAIDDWYIQDYQNYKSIVNNQSN
                     NYINGSEIYAKIALLTNSNTAGENKFISISNETVSKSYKPALFMTLSLILSVLFSSAYH
                     ITKRSVLEYKNKFNRSFDNL"
                     /name="hypothetical protein"
     gene            complement(4262..4375)
                     /locus_tag="MZH44_28670"
     CDS             complement(4262..4375)
                     /locus_tag="MZH44_28670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UTU32764.1"
                     /translation="MHEPPVQSDCCALSGNYQLESNPERHDKAPLAAATGN"
                     /name="replication initiation protein"
     Typing          205..378
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.571"
                     /coverage="100.0"
                     /evalue="2.6e-76"
     Typing          complement(2..238)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.958"
                     /coverage="96.0"
                     /evalue="8.55e-101"
     Typing          complement(4056..4532)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.714"
                     /coverage="95.0"
                     /evalue="8.08e-141"
     Typing          492..808
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.396"
                     /coverage="99.0"
                     /evalue="2.47e-61"
     Typing          805..2297
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.6"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agtcctgtcg ggtttcgcca ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg
       61 gggcggagcc tatggaaaaa cggctccgcc gcggccttat cgcttccctg ttaagtctct
      121 tcttggcatc tcctaggaaa tttccgcccc gccagtaagc cggtaccgct cgccgcagcc
      181 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga atatatcctg tatcgcatat
      241 tctgcttacg cgccggtgcc gccttttttc tccttccaca tgaagcactt cactgatttc
      301 cgcatccgtg ccaacatagt aagccagtat acactccgct agcgctacgt gactggttca
      361 gggctgcgcc ccgaaacccg ccaaaagcct gacgcgcctg cggcttgtcc aaccccgcgc
      421 cgaccgggaa aggctttccc gcccgtcccg gcgttatcag gaggccggtt tgtcagagaa
      481 acggaacaag atgctcacca tgtgggtgac ggaggacgag caccggcggc tgcttgagcg
      541 ctgcgacggc aggcagctgg cagcgtggat gcggcagacc tgcctggacg aaaagccggc
      601 acgttccgga aagcttccct caatctcgcc ggcgctgctg cgccagcttg ccggcatggg
      661 caataacctg aaccagatag cccgccgtgt taacgccggc gggggtaccg gccatgacag
      721 ggtgcagatt gttgcggcgc tgatggccat cgatgccgga cttgagcggc tgcggcacgc
      781 cgttcttgag aaaggaacgg acgatgatcg ttaaatttca tccacgcggt cgcggcggtg
      841 gcgccgggcc ggtggactat ctgctgggta aagaccggca gcgtgaaggc gccaccgtcc
      901 tgcaggggaa gccggaagaa gtccgggagc tgattgacgc gtcgccttac gttaagaaat
      961 acacctccgg cgtcctgtcg tttgcggagg ccgatctccc tcccggccag cgggaaaaac
     1021 tgatggccag cttcgagcga gttctgatgc ccggactcga taaagatcag tacagcattc
     1081 tgtgggtgga gcacgcggac aaggggcgtc tggagctgaa ctttctgatc cccaataccg
     1141 agctgctgac cggcaggcgg ctccagcctt actacgaccg cgccgaccgg ccgcgcatcg
     1201 atgcctggca gactatcgtg aacggcagac tggggctgca cgacccgaac gcgccggaaa
     1261 accgccgggc gctggtcacg ccgtccgggc tgccgaaaac gaagcaggag gccgcagaag
     1321 ccattacccg gggactactg agcttagcct cgtccgggga gctgaaaagc cgtcaggatg
     1381 tcactgacac tcttgaaggt gcaggttttg aggtggtgcg caccacgaaa agcagtatca
     1441 gcattgccga cccggacggg gggcgaaaca tccgactgaa gggagctatc tatgagcaat
     1501 ctttttacgg cctcgaggga cttcgaggag aaattgaaag cgcagcagca gagtaccggc
     1561 gagatgctga aagccgcatt cagcgagcac gagcagtctg tcagagcgga actgagcgca
     1621 agcgcgagga gaatcagcga cgccatcagc gcccacgagc agaatatgag cgaggcgatg
     1681 gagaaaaacc ggcggagcgt gctgctgacg gccgggagga catggctcac catcctgatg
     1741 gtgtcagcgc tgctgatcgg cacgagcggg agcattctgt ggtggcaggg gcagcagata
     1801 accgacaatt acacgcatct tcgtcagcag gaagataccc tggcgaaaat gaccgcccgg
     1861 acgtggggag tgcgctatca ggagagcagc gacggccgga gattccttat cctgccgccg
     1921 gggatgcaaa ccgaagccat cccttacgac gggacgacat ggatccgcct gaaacaggag
     1981 tagcggaaaa tgacagagct ggaaaagcag ttgctgagcg cattagagca gctacagcag
     2041 gactactcgc aaaggctgga cgaatgggag agcgccttcg cggaatggcg gagcatgtgt
     2101 ggtcttatgc aacgggagaa cgcgatgctg aacgagcgcg tgtcggactt gagcacgcag
     2161 gtgctgagtt taagcgagca gctgcgccgc ttgtccggga actgaatgcg atcgagctcc
     2221 ggcagaggga gaatgagtat cagaaaacac tggagctgga gcgccagccg cagaaaacct
     2281 accgcgggcc gacactttaa accatccagg cagctgacaa cccgcacgaa gaggggctct
     2341 tatttctccc gctaaatagg ttcgtgatca aaaaggtcac gacttattca ggagcgggaa
     2401 atgaagctgt taaatcttat tgaatcattg cttaactgcc tatacctggc ggcggagctg
     2461 tgggactggc tggagaagca tttgtcggtt tttaaggcat tggcgcggat tttagtgtga
     2521 ctttaacatc catgcatctg gctgcatgac tatgcaccag tgtaatattc agcggctgcg
     2581 tttctggtgt tttttcgtta tgtctgtcgc cactttctcg cgtctggcac cggaaacgca
     2641 gccaaccccg tttcagtggt gcggccaacg gatgttatgg taaatttcgg tgcattgtga
     2701 ctcgtttgga tatgttattt ggttgcagta ttgaacgacc cagaggatta aaaatggatc
     2761 tagcaaaagc actgaaggaa gcggaaaccg gatatagcct caacttaacc agtacagatg
     2821 aggctgaaat gctggcttat atctcaaccg tacttgaact ccacgctaaa ggtgagctac
     2881 tacatgagtc tgccatagac agggctatga cgttatgtgt gcttattaac aaaggaaacc
     2941 gacaggagtt aagccggacg cttcgcgggt ttaacaatgg tggtaactgg cctagcacta
     3001 cgggagcttc gtcaggctgc ggaaggggaa acgcttagcg ctgaaatccc ggaggtattt
     3061 aagatcttat cgaaaagccc actgatagta ggcttttctg catctggcgt atatcggatg
     3121 ttatgaggaa tatggttatt aaccgttata ggttatcaaa agaccggttg aatttgtttt
     3181 tatattcaag cacagatctt ttggttatat gatatgcgga tgaaaaaaga acagaaagaa
     3241 ttaaagatag agtcataaac aaggctggtt tgtaactttt gcttaccgtt tcattgctga
     3301 ttgaaatgaa tttattttct ccagcagtgt tgctgtttgt taacaatgca atcttcgcat
     3361 atatctcaga accatttata tagttgttac tctgattgtt aactatgctt ttataatttt
     3421 gataatcttg gatgtaccaa tcatcaatag ctcgatggat agtgtttact tcagcgatta
     3481 aaacaggttt gtcacggacg tcgccagacc atgaaaggtt tattttttgt gctttttcat
     3541 ctatgtttga tgttatgctc ttgcttttgt tgtttattat tgattgtatg gcgttcgctt
     3601 tacaataaaa atcatcccca caattaataa tcatgctggg tgaattgttg atcatgttta
     3661 attggtattt tatgattact ttgtcttttg ttataaacag gaaggtaaat gacaaaatta
     3721 atgagaaaat aacaaataaa atgatgctcg caatttttgt cttaagaaag agaaatagct
     3781 ctattaaatc aatatccgat gaattgtgtt gcattttcat acaccaatag tctttattaa
     3841 agcataagtt aaataaagga atgaaataaa aagagcagtg atgaaaattt ttgccgatgt
     3901 ggtgtatttc atttgaaagg ccaataagat aaaaaaaata ttttatccta ttatgtgttt
     3961 gtaggaaaag tcctaaaatg caagttttgt cgtttttttg ttcggttgtg cctgtggagg
     4021 gcattgttgt cagccaacat cctttccaca tattttaaga tcttcttgag atccttgcgt
     4081 tacagccgta atctcttgct ctgaaaacga aaaaaccacc tgccagggtg gttttttcga
     4141 aggttctctg agctaccaac tctttgaacc gaggtaactg gcttggagga gcacagtcac
     4201 caaaactgtt cgtttagttt agccttaacc ggcgcataac ttcaagacta actcctctaa
     4261 atcagttacc agtggctgct gccagtggtg ctttgtcatg tctttccggg ttggactcaa
     4321 gttgatagtt accggataag gcgcagcagt cggactgaac ggggggttcg tgcatacagt
     4381 ccagcttgga gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg agataaacgc
     4441 ggccataaca gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg
     4501 gagccaccag ggggaaacgc ctggtatctt ta
//
