LOCUS       CP100035                4677 bp    DNA     circular BCT 21-JUL-2022
DEFINITION  Escherichia coli strain EC204A1 plasmid p204A1-4K1, complete
            sequence.
ACCESSION   CP100035
VERSION     CP100035.1
DBLINK      BioProject: PRJNA827955
            BioSample: SAMN27625699
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Tang,B., Chang,J., Yang,H., Wu,J. and Li,J.
  TITLE     A 12-year investigation on the antimicrobial resistance of
            Escherichia coli from diseased swine
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4677)
  AUTHORS   Tang,B., Chang,J., Wu,J., Yang,H., Lin,J., Guan,C., Jiang,H.,
            Chen,H. and Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
REFERENCE   3  (bases 1 to 4677)
  AUTHORS   Tang,B., Chang,J., Yang,H., Wu,J. and Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. JAN-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/29/2022 22:18:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,744
            CDSs (total)                      :: 5,616
            Genes (coding)                    :: 5,416
            CDSs (with protein)               :: 5,416
            Genes (RNA)                       :: 128
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 200
            CDSs (without protein)            :: 200
            Pseudo Genes (ambiguous residues) :: 0 of 200
            Pseudo Genes (frameshifted)       :: 72 of 200
            Pseudo Genes (incomplete)         :: 131 of 200
            Pseudo Genes (internal stop)      :: 33 of 200
            Pseudo Genes (multiple problems)  :: 35 of 200
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC204A1"
                     /isolation_source="gut"
                     /db_xref="taxon:562"
                     /plasmid="p204A1-4K1"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2013-03"
     gene            518..631
                     /locus_tag="MZH44_28625"
     CDS             518..631
                     /locus_tag="MZH44_28625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UTU32760.1"
                     /translation="MHEPPVQSDRCAFSGNYQLESNPERHDKTPMAAATGN"
                     /name="replication initiation protein"
     gene            complement(866..1303)
                     /locus_tag="MZH44_28630"
     CDS             complement(866..1303)
                     /locus_tag="MZH44_28630"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UTU32761.1"
                     /translation="MPKRGKSIRSSSRNEETKPSKSTAKTTDRFISISFKHFHNVDDVG
                     QSINTWKDDGSLPDLIEKLVYLTANGITKVISDGVFTNYTRFPESAVNDFRCPDEISSD
                     EDWGVIKNIGGQKRRVAGFLRDNVFYIVFFDRDHRFWKSQK"
                     /name="hypothetical protein"
     gene            complement(1308..2606)
                     /locus_tag="MZH44_28635"
     CDS             complement(1308..2606)
                     /locus_tag="MZH44_28635"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="UTU32762.1"
                     /translation="MMVNFSLSNAFSFMEAQNMSMLAVSSSKDDINLANVVSVRDERIL
                     KSALIFGANASGKSNLMKTISLMKDIVLHSVQAIESGITKNVVPFLLDEAGPTKPSEME
                     MTFIADGIKYRYGLSLFQGKIKEEWLYYTPKTRETILFERDGQLIEINKAAFSESNLFI
                     KDGVIQKTREDVPFVSVVAGFNGEHSKRVVNWFSNVVFISGSNEGMFTGITMKLINESE
                     DFKAWLKKILPAFQINDIYLDEVAPEFPFGNIKTDDDNLKNLFSSIQNFAKNQKVFSLR
                     VVKKTANGEISVPIDFESEGTKKIVYLLGPIYNAMINNNLLLVDEFDSKFHTLLSKFLF
                     RIFHAQSEGFSQVIAAVQDVNLMNTDCFRRDQIWFVDKDVKSGSSHLYSLVEYKEKQRS
                     LKPSYGNDYLSGAFDAIPLFDNLNEFEKLMPEG"
                     /name="ATP-binding protein"
     gene            4364..4558
                     /locus_tag="MZH44_28640"
     CDS             4364..4558
                     /locus_tag="MZH44_28640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UTU32759.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQIRFEAESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(3850..4118)
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.037"
                     /coverage="93.0"
                     /evalue="7.52e-94"
     Typing          complement(4230..4328)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.0"
                     /coverage="100.0"
                     /evalue="1.35e-41"
     Typing          50..587
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.993"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 agtgtatact ggcttaacca tgcggcatca gtgcggattg tatgaaaagt acgccatgcc
       61 gggtgtgaaa tgccgcacag atgcgtaagg agaaaatgca tgtccaggcg cttttccgct
      121 tcctcgctca ctgactcgct acgctcggtc gttcgactgc ggcgagcggt actgactcac
      181 acaaaaacgg taacacagtt atccacagaa tcaggggata aggccggaaa gaacatgtga
      241 gcaaaagacc aggaacagga ggaaggccgc gccgctggcg tttttccata ggctccgccc
      301 ccctgacgag catcacaaaa accgacgctc aagtcagagg tggcgaaacc cgacaggact
      361 taaagatacc aggcgtttcc ccctggtgac tccctcgtgc gctctcctgt tcctgccatt
      421 cggtttaccg gtgtcactcc gctgttatgg ccgcgtttgt ctcattccac gcctgacact
      481 cagttccggg taggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc
      541 cgaccgctgc gccttttccg gtaactatca acttgagtcc aacccggaaa gacacgacaa
      601 aacgccaatg gcagcagcca ctggtaactg atttagagga gttagtcttg aagttatgcg
      661 cctgttaagg ctaaactgaa agaacagatt tggttactgc gctcctcata agccagttac
      721 cttggttcac agagcgccaa ctcttagaac cgtcgaaaaa ccgcccgcca aggcggtttt
      781 ttcgtttaca gagcaagaga ttacgacgag agcatcacaa tctcaagaag atcttttctt
      841 aacatacaat acaactgtat ctcatctatt tttgactctt ccagaagcga tgatctctat
      901 caaaaaacac gatatagaac acattatctc tcagaaatcc ggcgactctt cttttctgcc
      961 cgccaatgtt ttttataacc ccccaatcct catcagagga tatctcatct gggcatctaa
     1021 aatcgtttac agctgactca ggaaatcgag tgtagttagt aaacacaccg tcagaaatca
     1081 ctttagtgat tccattcgcc gttagataaa ccaatttttc aatgagatca ggtaggctac
     1141 catcatcttt ccatgtattt atggactgcc caacatcatc gacgttatga aaatgcttaa
     1201 aactaataga tataaatcta tcagtcgttt tcgctgtgct ctttgaaggt tttgtttcct
     1261 cattcctcga agagcttcga atgcttttgc ctctctttgg catataatca tccctcaggc
     1321 attagctttt caaattcatt aagattatca aataacggga ttgcatcaaa agcacctgat
     1381 aaataatcat tcccatatga tggcttcaat gatctttgtt tctctttgta ctcaaccaaa
     1441 gaataaagat ggctactccc tgactttaca tccttgtcaa caaaccaaat ttggtctcgt
     1501 ctaaagcaat cagtattcat taaattaaca tcctgaactg cagcgattac ttggctaaac
     1561 ccttcacttt gcgcatgaaa tatcctaaat aaaaacttag ataaaagggt atggaacttt
     1621 gaatcgaact catcaaccaa taataagtta ttgttaatca tggcgttata aataggccca
     1681 aggagataga ctatcttttt ggtcccctct gattcaaaat caatgggaac cgaaatctcc
     1741 ccattagcag tttttttcac gactctaaga gaaaatactt tttgattttt tgcaaaattc
     1801 tgaatcgagc tgaataaatt ttttaaatta tcatcatctg tttttatgtt gccaaacggg
     1861 aattcaggcg caacttcatc aagataaata tcattaatct gaaaggctgg taatattttt
     1921 tttagccaag ctttaaaatc ttcagattca tttataagct tcatggtaat tccagtaaac
     1981 attccttcgt tagaaccaga aataaatacc acattactaa accaattcac aacacgcttt
     2041 gagtgctccc cattaaatcc agcaacaaca gaaacaaagg gaacatcttc tcttgtcttc
     2101 tgaatgaccc catcctttat aaaaagattt gactcgctaa atgctgcttt atttatttca
     2161 ataagttgac catctcgctc aaagagaata gtctcccttg tttttggcgt gtagtacaac
     2221 cactcttctt ttatcttccc ttgaaataaa gataatccgt atctgtattt aatcccatcc
     2281 gcaataaaag tcatttccat ttcagatggt ttagtaggcc ccgcctcatc aagtaaaaaa
     2341 gggactacat tcttcgtaat gccactttcg atagcttgca cagaatgcag gacaatatct
     2401 ttcattaaag atattgtctt catgagatta gattttccgc tagcgttagc tccgaaaatc
     2461 aaagcagact tcagtatccg ctcatccctt actgacacta cattagccaa gtttatatcg
     2521 tctttcgatg acgacaccgc caacatgctc atattttgag cttccatgaa ggaaaatgcg
     2581 tttgaaagac taaagttaac catcataaga acacccctaa caacaccctt aaactcaaaa
     2641 attgcacaat attgacaaaa ttctagatta agaatagcat aaccccgtac taaaacaaca
     2701 aaagggtcca atagacagca cattatgagt agataagtta acgaccagct taaaaagagg
     2761 tgtatatgaa gttttttcta taccaaatgg tcccatttgc ctcagtgatt agctcagcag
     2821 tgctggcttt tcactcggta tctgggtggg gatggttcct ttttgtcgcg tttatgctca
     2881 gtggcgctga cactgtaaaa aaatacgtag ggagtgaaac atgaatgagg agaagcaaaa
     2941 attgttgaag taagtgggtt agcctgtacc accagaacta acccgtaaca aaccatacgc
     3001 aaatgcggcg cttgagtacc acctcaagtg ctttgataaa gccagccaca agatctgttg
     3061 gttgctacag tacaattttg atagcaaaaa tcaacattca tgtggcgaaa ataggaaatg
     3121 caacatgcag acggacgagc ctcaggcata catttttcgc ccttacataa cggtaaaggg
     3181 caaacggata acccgtccta acgggggaat gtttaagatc ccaattaatc gggaaaagaa
     3241 aaaacaataa ccaccgaagc atgcaagctt cggtccctaa cttagtttat gtaaggaaag
     3301 caacatggcc ttttatgtag atagcacagc gaaattttta acaaaaacaa ccgagcctga
     3361 agggagtgta tggcggtatt actataagcc gggaaatact tgtccggctt ccggcattta
     3421 ccgctgcacg aactgtggtg atgaagtgac gtgcaataaa gatgatcctt tgccaccgca
     3481 aaatcatagg cagcataatg tcccaggtga catccgatgg gaattggtag tgatgacaaa
     3541 gacgaagtaa cttactaggc cgtctacgga cggctttttt atacctgcca atcagctgga
     3601 tttaccataa catccgattg cgcgcaccgc taaaacgcgc tgaacgcgat tctgccgcaa
     3661 acaccgcaaa aagagatgtt ttccccgggg taaaccggaa aaatgcgtca ggctcgcttt
     3721 cagctcactg catagctatg catgaaagtg aatgacgatc ggtttgggcg catacagtgc
     3781 ccatgagaca cattttcgga cattttcagt tcgtgaatcc ctgactcatt gctacatatc
     3841 tgaacagaca cccctggcag cgcccccagg ggcgtcctgt aaaaaaacac accgcgcagc
     3901 tagcagcacc cctaatataa gatgatgttt tttatgaaaa tagtcagtac cacccctaaa
     3961 aagcggtgtc ggcgcggtgt tgtagctgcg ccaacaccgc ttttttaaat atcacaaaga
     4021 gagcaagaag aactaatttg tcataactct ctatttataa agaaaaatca gcaaaaaatt
     4081 gtttttgcgt ggggtgtggg catttcgggc atgagaacag actcgcgttt ttccggagta
     4141 tcagcggaat tattgattaa gaaacactcg acgcagagcg aaattttatg caacagcggc
     4201 tgcactgcac agggattatt tatcagttga tatgctgtgc catgatacgt cctaaagaaa
     4261 caggacagga tgtgcaattg taataccgtc gcccgcaacg tcttacaatt accacctgct
     4321 cagggtcccc ccgacacccg gaactgacgg agaacaacgg aaggtgaaca aacaacaaca
     4381 aactgcgctg aatatggcgc gatttatcaa aagccagagc ctgacactgc ttgaaaaact
     4441 ggatgctctg gatgccgacg aacaggcggc catgtgcgaa cgactgcacg aactcgcaga
     4501 agaactccag aacagcatcc agattcgctt tgaggccgaa agtgaaacag gaacataacg
     4561 aagctgacgg aggcggtcac ggctttcccg tgggcgtttt cgtcagcaga caagcccgtc
     4621 agggcgcgtc agggggtttc ggggcgaagc cctgaaccag tcacgtagcg atagcgg
//
