LOCUS       CP100034                5128 bp    DNA     circular BCT 21-JUL-2022
DEFINITION  Escherichia coli strain EC204A1 plasmid p204A1-5K2, complete
            sequence.
ACCESSION   CP100034
VERSION     CP100034.1
DBLINK      BioProject: PRJNA827955
            BioSample: SAMN27625699
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Tang,B., Chang,J., Yang,H., Wu,J. and Li,J.
  TITLE     A 12-year investigation on the antimicrobial resistance of
            Escherichia coli from diseased swine
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5128)
  AUTHORS   Tang,B., Chang,J., Wu,J., Yang,H., Lin,J., Guan,C., Jiang,H.,
            Chen,H. and Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
REFERENCE   3  (bases 1 to 5128)
  AUTHORS   Tang,B., Chang,J., Yang,H., Wu,J. and Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. JAN-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/29/2022 22:18:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,744
            CDSs (total)                      :: 5,616
            Genes (coding)                    :: 5,416
            CDSs (with protein)               :: 5,416
            Genes (RNA)                       :: 128
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 200
            CDSs (without protein)            :: 200
            Pseudo Genes (ambiguous residues) :: 0 of 200
            Pseudo Genes (frameshifted)       :: 72 of 200
            Pseudo Genes (incomplete)         :: 131 of 200
            Pseudo Genes (internal stop)      :: 33 of 200
            Pseudo Genes (multiple problems)  :: 35 of 200
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC204A1"
                     /isolation_source="gut"
                     /db_xref="taxon:562"
                     /plasmid="p204A1-5K2"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2013-03"
     gene            join(5092..5128,1..95)
                     /locus_tag="MZH44_28585"
     CDS             join(5092..5128,1..95)
                     /locus_tag="MZH44_28585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UTU32754.1"
                     /translation="MHEPPVQSDCCACSGNYRLESNPERHDKAPLAAAMGKGVYKIY"
                     /name="replication initiation protein"
     gene            344..1228
                     /locus_tag="MZH44_28590"
     CDS             344..1228
                     /locus_tag="MZH44_28590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="UTU32755.1"
                     /translation="MAVTVGFISEKGGVGKTTSCYHIAEGLSRFHEKRVLVIDADYQRG
                     GITGRFFPKLIENFGNQAPQGTTLFNKYQQLYSAAQQTTDIDIIKFNSGIDVIPADPRL
                     STVSTDKLPSTNNIRSNNAILLQHLKTISAVLSPIADNYDYILIDSHPEVSDVMRSIIY
                     ASDYCVSPVKLDRQSSIGVATVIGEINNVNEDIEMLRKALQVGDSYKDTVFAGAMGMMA
                     REYAEELKQTEQYEYNRLRQAGDIFKHYITEGDGLRVAAAERVSVYDVQISNAYKQASQ
                     FKNLTIEFMRTCR"
                     /name="ParA family protein"
     gene            1225..1626
                     /locus_tag="MZH44_28595"
     CDS             1225..1626
                     /locus_tag="MZH44_28595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UTU32756.1"
                     /translation="MIDIQKLISWLGVEGAKAGLDKSEMTNAELIESFGNLLPKNPSKL
                     KRSDLVEEIILATRRMTHKSVEELMEMSKEDLYSYFHDQKYSRKELLDLLYTLEIRPGS
                     SAKKNLTEFTISEISDIGMYRRVAKGNHA"
                     /name="hypothetical protein"
     gene            complement(2446..2679)
                     /locus_tag="MZH44_28600"
     CDS             complement(2446..2679)
                     /locus_tag="MZH44_28600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UTU32757.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNEQVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2686..3204)
                     /locus_tag="MZH44_28605"
     CDS             complement(2686..3204)
                     /locus_tag="MZH44_28605"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="UTU32752.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVLFLFTVSG
                     GTLWYLGGRIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVLPYGKSATVIPYQ
                     EKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            complement(3242..3673)
                     /locus_tag="MZH44_28610"
                     /pseudo=""
     CDS             complement(3242..3673)
                     /locus_tag="MZH44_28610"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3882..4205)
                     /locus_tag="MZH44_28615"
     CDS             complement(3882..4205)
                     /locus_tag="MZH44_28615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UTU32758.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQVVREHGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4270..4461
                     /locus_tag="MZH44_28620"
     CDS             4270..4461
                     /locus_tag="MZH44_28620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UTU32753.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(4557..4730)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.571"
                     /coverage="100.0"
                     /evalue="1.23e-64"
     Typing          4697..5128
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.843"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          complement(2343..3892)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.985"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gttccggtaa ctatcgactt gagtccaacc cggaaagaca cgacaaagcg ccactggcag
       61 cagccatggg taaaggggtg tacaagattt actgagtgag agttcttgaa gtatcagcct
      121 tagagaggct aaactggaat aacagaattt ggtttcagcg gtcttgtaca ccctgttacc
      181 acggttcaga agttccccaa cttactgaac cctcgaaaaa accgactccc cagtcggttt
      241 ttttcgtttg atggctaaga gtacgctgac gttatactga acgtcaagga aagcgttgac
      301 gctgttccct ctaccaaaaa aaagagattt gtaaggaata aaaatggctg tgacagttgg
      361 gtttattagt gagaaaggtg gggttggtaa aacaacgtcc tgttatcaca ttgccgaagg
      421 acttagtcgt tttcatgaaa aacgtgtttt agttatagat gcagattatc aacgcggggg
      481 tataactgga agatttttcc ctaaactgat cgaaaacttt ggaaatcaag caccgcaagg
      541 aacaacgtta tttaacaagt atcagcaatt atatagcgcg gcacaacaaa cgactgacat
      601 agacataatt aaatttaata gtggtatcga tgtcattcca gccgaccctc gcttatcaac
      661 cgtttcaact gataagctac ctagtacaaa caatataaga tcgaacaatg ccattctttt
      721 acagcattta aaaaccataa gcgctgtatt atcacctatt gcagataatt atgactacat
      781 attgattgat tcacaccccg aagtttctga tgtcatgcgc tctataatct acgcatcaga
      841 ttattgtgta tcaccggtaa aactagacag gcaatcatct attggtgtgg ccacggtcat
      901 aggtgagata aataatgtaa atgaagatat tgaaatgcta agaaaggcac tgcaagtcgg
      961 cgattcatat aaggacacag ttttcgccgg agcaatggga atgatggcca gagaatatgc
     1021 agaagaactg aagcaaacag agcaatatga atacaacagg cttcggcaag caggtgacat
     1081 cttcaagcac tacataacgg aaggtgatgg gctaagagta gctgcagcag agcgcgtttc
     1141 agtgtatgat gtacagataa gtaacgctta caaacaagcg tcacagttta agaacttgac
     1201 gattgagttt atgaggactt gtcgatgata gatatccaaa aactgatatc ttggcttggc
     1261 gttgagggag ctaaagctgg cttggataaa agtgaaatga ctaatgccga gctaattgaa
     1321 tcctttggta atttacttcc aaagaaccca agcaaattaa aacgctccga tctcgttgag
     1381 gagattattt tagcaacaag gagaatgaca cataaatcag ttgaagaact gatggaaatg
     1441 tcaaaagaag atctgtattc atatttccac gatcagaaat atagcagaaa agaacttctt
     1501 gatttgttat atactcttga aatcagacct gggagctcag caaagaaaaa tctaaccgag
     1561 ttcacaataa gtgagataag tgatattggc atgtatcgtc gcgtagcgaa agggaatcat
     1621 gcataaataa aagccacctg tgaggtggct ttttatagga taaaaaaatg ccatttaata
     1681 taggcgatat agtttactta aagtcagaat caaaagcgat gacggtcatc ggatatggat
     1741 cgtctcaaaa taacggttac atagatctag ctaattgtgc ttggctagat aaagacaacc
     1801 gctattgcca aatactattc ttgtttcaaa cgatcgaaaa aagaatcagt taaaaactgg
     1861 cttatcttca ttgagcatac cggacgaacc tatttaccat aacatccgtt gcccgcaccg
     1921 cagaaacgcg ttcggtgcga tcacggcagc agacaggtaa aaatggcaac aaaccacccg
     1981 aaaaactgcc gcgatcgcac ccggtaaatt ttaaccacat gcatagctat gcagccatgt
     2041 gaatcacgca ggaattgcca gccagagaca gctgaaacgg atttttttca tgctttcgga
     2101 agcgaagaag acggggaccg gagcgggaaa acagaagttc aggggaatga gcgcgatctg
     2161 gcaatagagg caaaacacag caacaaaaga cacaccagaa tcgcgcccgt atgcgtttta
     2221 acgcgttcct gtgttttcag caaggtctga ctgaagcgcg ttcccggtgc ccccgaacca
     2281 accattatcc caaaaaatgc accggaaacg cgctgacaac attttagcct gtctctgatt
     2341 accatcccag cgaagggcca tccagtgtcc gttcctgtat ttccggctgg cgctcacgct
     2401 ccagggataa ctcatgttcg cgctcctgta tcagccgttc gtcccttatc tcctgttctc
     2461 gctgtataac tggctcaagc gttctgtccg ctcgctcaag tgctgcacct gctgactcaa
     2521 ctgcatgacc tgctcgttca gcatcgcgtt gtcccgttgc gtaagcgaaa acattttctg
     2581 caattccacg aaggcgctct cccattcgct cagccgctgc atatagtcct gttgcagttg
     2641 ctctaaggcg ttcagcaaat gtctttccag ctccgtcact ctgtgtcact ccgtcagttg
     2701 tacccagtcc ttctcctgat agggaatcac cgttgccgat ttcccgtacg gaaggaccag
     2761 aaatttcctt ctcccgtcct gcacaaactc cacgccccat gtcttcgcgt tcagtttctg
     2821 caatgtttct tcctgtatcc tgatttcttc caggttcgcc tgtatccgcc cgccaagata
     2881 ccagagcgtc ccgccactca cggtaaacag gaaaaggacc atccccagta acatcatgcc
     2941 cgtattccct gccagcttta acacgtcctt cctgtgctgc atcatcgcct ctttcacccc
     3001 ttcccggtgt tttttcagtg attcctctgt cgaagctgtg aacagggcta tagcgtcttt
     3061 gattttcgtc tcgtttgatg tcacagcctt gcttacagat ttttcgagct tgctgaactc
     3121 gtcgttcagc attgtctctg tagattcggc tctctctttc agctttttct cgaactccgc
     3181 gcccgtctgt aaaagattgc tcatagaatg ctcctttcag tctgatattc ttcccgccgt
     3241 tcgggtctgc aatgctgata ctgcttcgcg tcaccctgac cacttcaagc cctgcctctg
     3301 tgagcgcctg aatcacatcc tgacggcctt ttatctcccc gacatgataa agagcgtcta
     3361 tcccgcgtgt gacgctctct gcaagcgcct gtttcgtttc cggcaggtta tcagggagag
     3421 tcagtatccg gcggttctcc ggggcgttgg ggtcatgcag cccgtaatgg tgattaacca
     3481 gcgtctgcca ggcatcaatt ctcggcctgt ctgcccggtc gtagtacggc tggaggcgtt
     3541 ttccgctctg tagctccatg ttcggaatga caaaattcag ctcaagccgt cccttgtcct
     3601 ggtgctccac ccacaggatg ctgtactgat ttttttcgag accgggcatc agtacacgct
     3661 caaagctcgc catcaccttt tcacgtcctc ccggcggcag ctccttctcc gcgaacgaca
     3721 gaacacctga cgtgtatttc ttcgcaaatg gcgtggcatc gatgagttcc cggacttctt
     3781 ccggattacc ctgaagcacc gttgcccctt cccggttacg ctcccttccc agcaggtaat
     3841 caaccggacc actgccaccg ccttttcccc tggcatgaaa cttaactatc atcccgcgct
     3901 ccatgttccc tgacgacctg ccgtaagctg cgcaactctc tctcgatggc catcagtgcg
     3961 gccaccacct gaacccggtc accggaagac cactgcccgc tgttcacctt cctcgccgtc
     4021 tgattcagat tattcccgat ggcagccagc tggcgcagta acggcggtgc cagtgtcggc
     4081 agcttcccgg aacgggcaac cggctcaccc agacagaccc gccgcatcca taccgccagc
     4141 tgtttaccct cacagcgttc cagtaaccgt gcatgttcat catcagtaac ccgtatcgtg
     4201 agcatcctct cgcgtttcat cggtatcatt accccatgaa cagaaatccc cccttacacg
     4261 gaggcatcag tgactaaaca ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc
     4321 cagacattaa cgctgctgga aaagctcaac gaactggacg cggatgaaca ggcagacatc
     4381 tgtgaatcgc ttcacgacca cgccgatgag ctttaccgca gctgcctcgc acgcttcggg
     4441 gatgacggtg aaaacctctg acacatacag ctcccggaga cggtcacagc ttgtctgtga
     4501 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag gccgttgacc ttgcgggtgt
     4561 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttactatgt
     4621 tggcatgagt gcgggtatca ggaaagtgct tcatgtggca ggagaaaaaa ggcggcaccg
     4681 gagcgtcagc agaatatgtg atacaggata tattccgctt cctcgctcac tgactcgcta
     4741 cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg aacggggcgg agatttcctg
     4801 gaagatgcca ggaagatact taacagggaa gcgatagggt cgcggcgaag ccgtttttcc
     4861 ataggctccg cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa
     4921 acccgacagg actataaaga taccaggcgt ttccccctgg tggctccctc gtgcgctctc
     4981 ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttatctcatt
     5041 ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa
     5101 ccccccgttc agtccgactg ctgcgcct
//
