LOCUS       CP100033                5179 bp    DNA     circular BCT 21-JUL-2022
DEFINITION  Escherichia coli strain EC204A1 plasmid p204A1-5K1, complete
            sequence.
ACCESSION   CP100033
VERSION     CP100033.1
DBLINK      BioProject: PRJNA827955
            BioSample: SAMN27625699
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Tang,B., Chang,J., Yang,H., Wu,J. and Li,J.
  TITLE     A 12-year investigation on the antimicrobial resistance of
            Escherichia coli from diseased swine
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5179)
  AUTHORS   Tang,B., Chang,J., Wu,J., Yang,H., Lin,J., Guan,C., Jiang,H.,
            Chen,H. and Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
REFERENCE   3  (bases 1 to 5179)
  AUTHORS   Tang,B., Chang,J., Yang,H., Wu,J. and Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. JAN-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/29/2022 22:18:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,744
            CDSs (total)                      :: 5,616
            Genes (coding)                    :: 5,416
            CDSs (with protein)               :: 5,416
            Genes (RNA)                       :: 128
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 200
            CDSs (without protein)            :: 200
            Pseudo Genes (ambiguous residues) :: 0 of 200
            Pseudo Genes (frameshifted)       :: 72 of 200
            Pseudo Genes (incomplete)         :: 131 of 200
            Pseudo Genes (internal stop)      :: 33 of 200
            Pseudo Genes (multiple problems)  :: 35 of 200
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC204A1"
                     /isolation_source="gut"
                     /db_xref="taxon:562"
                     /plasmid="p204A1-5K1"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2013-03"
     gene            complement(join(4906..5179,1..653))
                     /locus_tag="MZH44_28560"
     CDS             complement(join(4906..5179,1..653))
                     /locus_tag="MZH44_28560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="UTU32748.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTAEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            830..1228
                     /locus_tag="MZH44_28565"
     CDS             830..1228
                     /locus_tag="MZH44_28565"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UTU32749.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKEIKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            complement(1422..2357)
                     /locus_tag="MZH44_28570"
     CDS             complement(1422..2357)
                     /locus_tag="MZH44_28570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009440807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UTU32750.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAIERALCEKLGADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDKRFMAL
                     CMLENGYSQKAIAAMLNVHRNTIRNWAMHK"
                     /name="replication initiation protein"
     gene            4095..4304
                     /locus_tag="MZH44_28575"
     CDS             4095..4304
                     /locus_tag="MZH44_28575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UTU32751.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     gene            complement(4370..4903)
                     /locus_tag="MZH44_28580"
     CDS             complement(4370..4903)
                     /locus_tag="MZH44_28580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UTU32747.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     Typing          complement(1423..2357)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.872"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4..653)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.696"
                     /coverage="99.0"
                     /evalue="5.92e-142"
     Typing          complement(4910..5178)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.778"
                     /coverage="99.0"
                     /evalue="5.24e-49"
ORIGIN
        1 gcttcaaaac ccagatgcac ttcgggacgt cgttcaatgc cctgttcttt cagcgtccgg
       61 cagtcaatga agcccctttc aaagccatgc tttctcatat gttcgttgtg cttcacctcc
      121 cagcgttttc gcaattcccg caactgctct ttttgctgag tgagagtcag gcttccggaa
      181 tcctttttcg cacctccccg ctcgggatac ctggcgttat agcgccggaa atactgttcc
      241 ggtgtacgtt caatgccatc attcacacgc tggctcatca tgatatgcgc atgaggctgt
      301 tcgccgccgt caatgctcgc tttcggattg tgaatggcaa aactccaggc gtgccgttcg
      361 ccagcttcct gacgtacaaa atcctgcacc agttcaaggc gctgttccgc tgtcagttca
      421 cggggtaatg caatttcaag ttcccgatag gtcgaaccgt tcgcacgttc aaactggtca
      481 gcagcctgcc agaaatgtga gggattatct cgcgcccatt ccggcatgtt tccgtgtgcg
      541 gaatattcca aatcctgacg gtcgcggtat ttctctttgc gctcgatgta atcggcgtgg
      601 tttgcagcct gccccttgcc accaaattta acgctgaggt gataggttgc cacggtttca
      661 gccctccgca ggagcgcact gtttctcgac gaaggagaga aacatataag tgcgcttcct
      721 tctaatttta cgaattagca cggaagatac cccctgcccc cgccgaccgc aagcgatata
      781 ctgcacgacc acaacacacc gcaaaaaaac accagggagc atcacgaaca tgcgcatcag
      841 caacatcgaa tggctgaaga aacggatcgg attcatcagg aagctcggag aacagaccgc
      901 acgacagaga cagattattg acctgctcga caacgaagca ggactcacag aacaggaacg
      961 caaactcctc cacgtactgg caacagcgga aaaaaacgac ttacaggcac aggagagcga
     1021 acgaaaacag gcggtccaaa aacgaatcga gggaaaaaaa caacgacggg aacgcaatca
     1081 ccgactcttt ctcgcggctg ggctactgat cgaagcagga ctggtagaca caaaaacagg
     1141 cgagctatgc tataaaaaag acaggatatt acaggcactt aaggaaatta agtacgatct
     1201 ggagacatcc ccaaacccag acgcgtaagt gcctccgttc acgttccggt tccctggtgc
     1261 ttcctcgctc attcgctcgc tacgctcggt catgagactg cggcgagcac gaccacctca
     1321 atcatgcggt aagccgcacc attactgggc tgaaagggga aaaccgccgg aaagcccact
     1381 gctcaaaaag acgccagaaa agcgctgtta tctgatatag gctatttgtg cattgcccaa
     1441 ttacggatcg tattacgatg tacattcagc atcgccgcaa tagctttctg agaatatcca
     1501 ttctccagca tacatagcgc catgaaacgc ttgtcatcgt aggcttctcc tttagcgatg
     1561 ccacctttcc ggcctctctt ggcctgtatt tctggtgtgt gcgtatcagc cacatactgt
     1621 gcgaaagttt ccggcgtgaa gttcctgtgc gtgtatttcg caatgctctt gccaatagcc
     1681 cgacattcag ccagtgaaag cggaacggga agcgatgcgt tgtacatttc gacacgctgg
     1741 atcaccgctt caagccactg tgagaacgct ggccagccct gacgaatcgc cctgtaagcc
     1801 catttgcgcg tcatttcgaa caagtggcag tttcgcccca tcccgtaatg tttatcgatg
     1861 ctacgacgct ctgaggcgct caaatcgaga taatcagcca gttcatcgag ggtataggct
     1921 tcctcgcgcc actccatcac ctgccattcc aggtggaacg gatttttgca aatcagtccg
     1981 ctgtagttca catcagcgcc aagtttctca cacagcgcac gctctatcgc ggcggcgtac
     2041 ttcagtgcct taaccgacgc atcaggcgcg gttctcacgg cgatgttgag tgcatagagc
     2101 aggtgagcgt gtccgttcac aggattttta acggtgatgt tcggcgcggg ggcgttccgg
     2161 tcgctccagt caatcgcagc cccctcgcgg tcaacatcaa aaaccagcca gaactgcgca
     2221 tgaggctggt tctgctgtat gtaccgcgca agaagcgcac gccctttgcc ggagatgcga
     2281 agaccaaaag cgagatcatc cgtgtgatag ggacggtggg gtaaattttc ttcgaagtat
     2341 tgaagcgcgg cgctcatgtg ttacgattcc ttccaggtta tcgaccgaca tcgcccctgt
     2401 attttcgtga aagtcttggc ggatagacac gaatttcagg ggcgtttgtt tttcagtcgc
     2461 ttacactacc cccaacgaca aaacctgtcg agccttcccg caaatccgat ccatttacca
     2521 taactcccgt tgtacgtacc atgtaaaacg ccctcaatcg ctttctggcg caaaaaacgc
     2581 aaaaagacaa gaaaacacca ggccaaacca gaaaaacgcg tcagaatcgt tttcagggcg
     2641 ttgcataact atgcacgaaa gcgatgtgcg atcggtttcg tgcgttctga acaccgaacg
     2701 gctcaaaacg acaagttttc tcccctggtg cgtggcgctc tgacggggtt tcctgtttcc
     2761 aaacttcaca tctcgccagg acaaaaaaaa ccgacctggg gtcggtggca agaatcagag
     2821 tctatgggaa atattttagt tatcggatgc tcaatattca caaacgcaaa aaatgcctca
     2881 gtgatgccct aaaacaacct ttccagaagg agtccgcgct ttacgatgca cggaagagat
     2941 aaaaaacccc acaaaagtgg ggaaccaaga gaaacaacaa ttacaggttg ttggttgtgt
     3001 tcgaacaacg atgaagtagc tacgctaaat aataattacc gtacaggccc tacatattca
     3061 acactaaatt taagatcctg acgaaggatt tataaatgca cgaaaacaca tagacagaca
     3121 aattcactaa attcatacac aaaaacaagt gtagttttct aagtaaatta gattcgacaa
     3181 ccaattagtt tatccacatc gcattccact cttgctacac caatcacatt tcttgatatg
     3241 taatagcaga aaaaataaaa ccagacacca atagaccaaa aaacaaacat tcaacaagaa
     3301 acattttgaa caatatcaca aaactgtcac aaccatcatc agactattga aaaaaactgg
     3361 ttgctgtttt ataaaagcga ctgtaacaac ctccactcta tagttccatt agtttaaagt
     3421 gtcactgtca cacaaactga gcatccattt gttttcctta cttttgcccc tgtattccaa
     3481 ggggtttttt tataacttaa agattagcac accacacaaa aacaataaaa aaacgacaag
     3541 ttatacaata aaaccagtac tatccataca gctaattaca tataaatact attaaaccat
     3601 ctggacatta aaaataaact cttataacat aaaattaatg cggttccggt accatatgtc
     3661 agggataatg tgcaaatgga ggtatcggaa ctgcatttca gttcattatt aattaatgac
     3721 aaaaaaaacc tctgcgagcg cagagggggt aaggaatgtt cccagtcata atatgtcgat
     3781 agtgtttata tacatacaga acaagaacaa caaccagcca actaataaat attttatttt
     3841 ttagagctga aaccttaaag ctcacttcca ttccatacat tgttataaac cttatgttga
     3901 aaactgaaag taagtacggc agacttatta agttgcgtgc agtcttttca cgttatacaa
     3961 acaagaacac ctgcgaagtt atttctcctt acatatcccc ctatgccaac gggggatttt
     4021 ttctggttca ctaacgcata aataatagtg acaccctatc attcacaaac cgtgataata
     4081 taaggattat catcattgca aaattaggtg aaaacacccc acatattatc gatgctactg
     4141 tagattttat ggcatcaagc caggcatttc gtgagtatct gaaaaaactt ccccctgata
     4201 acacaatacc gccagctctt actgatgagc acgtaccgtt atatatacga aggctggaat
     4261 actatcgtct gctttatcga ccaaagcaga cagaatcata ttgaaaaaaa cggtgaacgg
     4321 ttcgcgaaat cctttcgcgg gtcgtgaggc gttggacaag cgaagcgcgt cagtttttcc
     4381 ggctcttccc cgtgacaggt ttctccacca gtttatcttc cagtgtcaca actttctgat
     4441 acaccgccgc tgtggcgttg tagttcctgt cgagtattgt tttcattccc catccgagca
     4501 cacagaacat aaccagaaaa gccactgaca ccccacccag ccatttccag ctataccctt
     4561 taagggtgtt gtattcctgc acgatattgc tgtgaattgt cgcaatatcc ctgttcaggg
     4621 catccgcgtt tgccttcatg tttgcgactg actcttccag ccgctgaaaa tcctggctga
     4681 tgcgctcacc tatcagtcct gccacgtcca gcttctgcac ctcatttctg acttcatccc
     4741 tgacctgttt tgccacaccg tcagaaatat cccgatatcc gcgaagagtt tcccttgcca
     4801 cagccagaga atcagcaaca gctttctgtt gctccattgc ggtatgttgc agatgctcca
     4861 ccgataacag gagcgtgagt aatttttctt ctgtgctggc catgttcaga atcccataga
     4921 aaatccacgt gacggtgatt taacccgttc aggtgcagtt cggttctgtc tgctcactct
     4981 gtctctcatg tcaaagccat gctcccgcgc aacatcatgt tgcagccttc tgaccagttc
     5041 atgttgctta accggagtgt ccagccattc cctgatgtca tgcccgaccg ttcgcgcctg
     5101 ttcaagatgg cgggaatagt ccggttcaga ccgtttttgc atgatgtcat gacgctgggc
     5161 atcatcaagc cgtccggca
//
