LOCUS       CP100024                2042 bp    DNA     circular BCT 21-JUL-2022
DEFINITION  Escherichia coli strain EC602A1 plasmid p602A1-2K, complete
            sequence.
ACCESSION   CP100024
VERSION     CP100024.1
DBLINK      BioProject: PRJNA827955
            BioSample: SAMN27625687
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Tang,B., Chang,J., Yang,H., Wu,J. and Li,J.
  TITLE     A 12-year investigation on the antimicrobial resistance of
            Escherichia coli from diseased swine
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Tang,B., Chang,J., Wu,J., Yang,H., Lin,J., Guan,C., Jiang,H.,
            Chen,H. and Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
REFERENCE   3  (bases 1 to 2042)
  AUTHORS   Tang,B., Chang,J., Yang,H., Wu,J. and Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. JAN-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/29/2022 22:33:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,933
            CDSs (total)                      :: 5,804
            Genes (coding)                    :: 5,484
            CDSs (with protein)               :: 5,484
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 98
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 99 of 320
            Pseudo Genes (incomplete)         :: 227 of 320
            Pseudo Genes (internal stop)      :: 56 of 320
            Pseudo Genes (multiple problems)  :: 55 of 320
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC602A1"
                     /isolation_source="gut"
                     /db_xref="taxon:562"
                     /plasmid="p602A1-2K"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2018-03"
     gene            complement(join(1052..2042,1..26))
                     /locus_tag="MZH69_29660"
     CDS             complement(join(1052..2042,1..26))
                     /locus_tag="MZH69_29660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001707479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="UTU27359.1"
                     /translation="MRSPDWLDVRQNEGVSMANYAIMRCKKLTGMGSVASALQHCYRER
                     ETPNADAERTPENYCSVSQSADEAMGKLRELLPEKRRKDAVLAVEYVMTASPEWWNEAT
                     PRQQAEFFARSEQWLENKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGG
                     RSKMRDDQSTYAESVKKLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPR
                     VLRKGIFTKDVEDQAAIAKRLSQAVNDGFAGTVAMASQSAQNAKRARELQKTMDSQQKR
                     LQSVTEPFKGLSREQMTEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMSR"
                     /name="plasmid recombination protein"
     gene            557..988
                     /locus_tag="MZH69_29665"
     CDS             557..988
                     /locus_tag="MZH69_29665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="UTU27360.1"
                     /translation="MRRITQVDQSTGEELGGFVAVIRPKQKSSFQRHFTMNQAALITIA
                     NELNHDQMRVLMALLAELDYENYIQVAQIDIAEALTMQKTNVSRAVKNLIDFGIILEGP
                     KIGRSKTYRLNPQFGWKGTVSNHKKALKNGLSVIQGGRT"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(1056..2041)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.264"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 acatccagcc agtcggggga tcgcattgcg atgcctccgg cgggccacta tcaggatgat
       61 agtgtactca ctagactttg ctacgcaaaa tcgttcgggc agcaagctgc cagaaggtca
      121 aaatcaacgg cagtctggca ggtggaaggc gtcgctccgc gccacccccc accactgcca
      181 agcctgtttt atccggtgag tcgtcaaggg taaatggcac tcgcttcgct cgcccctgac
      241 gaaatcaggc aatgaatatc gtttttgaaa gagcaggaac cgctcgccgc aagaacggat
      301 tagggagcgg catcgccgcg agtctgtccg ttcgaggaag cggaatttcc actccccgca
      361 catagaaatt cggacactca atactggcag aaaaacacct aattttgccg atgtatgagt
      421 acggagagta ataagataca gaaggggatc tctcggaagc cgcgccacaa gcggaaagtt
      481 tttgacaata gttatctcta gagataactt agttatcttg agagataact attttttccc
      541 gaatggagat ttttagatgc ggagaatcac gcaggtagac caaagtacag gggaggaatt
      601 aggtggtttt gtcgcagtga ttcgtcctaa acaaaagtca tcattccaga ggcactttac
      661 tatgaaccaa gccgcgctaa ttaccattgc caatgagcta aaccatgacc agatgcgtgt
      721 gctgatggcc ttgctcgcag aactggatta cgaaaactac atccaagttg cgcagataga
      781 tattgcggaa gcgctgacta tgcaaaaaac aaatgtcagt cgtgcggtaa aaaacctgat
      841 tgatttcggg atcatactgg aaggaccgaa aattgggcgg agcaaaacgt accggctaaa
      901 tccccaattc ggctggaaag gtactgtcag caaccacaag aaagcactaa aaaacggcct
      961 cagcgttata cagggcggca gaacctgatc tctggaatgc cgcaaatgac ctcgttttgc
     1021 ggcgtcagac ctatcctgca tcgcatcaaa actaacgact cataccaccc ataccacgcg
     1081 atcgcgtctg tcgctgtttt tcacgttcaa tacgctgctg tttttccttt tcctggtttt
     1141 gctgcttaaa ccgctgagcc atcatcagga tttcggtcat ctgctctctg gaaaggccct
     1201 taaaaggctc tgtaacgctc tgtaggcgtt tctgctgcga atccatagtc ttctgtagct
     1261 cgcgcgctct ttttgcattc tgggcgctct gagaggccat agcgacggtt cctgcaaagc
     1321 catcgttcac tgcctggctt aaacgtttgg ctatggctgc ctgatcttcc acgtctttgg
     1381 tgaaaatacc cttccgcagt acacgcggct ccagcgcctc cggtgagatc gatacctggc
     1441 tacgggtgcc acggtttatc gattcgtagt agtgctgaac tgtctgatgc gtggcacggc
     1501 ttccctcgat gccgcgctca agacccagtt tttttaccga ttcagcgtag gtgctctgat
     1561 cgtcacgcat ctttgaccgt ccgccaataa actctttcgc ggacagccgc ccatcctgcg
     1621 tcaatggcac cacgaacgca gacaggtgcg gggtggcctc gtccctgtga accaccgccg
     1681 ccacaacgcg atctttcccg tatttgttct ccagccattg ctcagaacgg gcgaaaaact
     1741 ccgcctgctg ccgcggtgtg gcctcgttcc accactcagg gcttgccgtc ataacgtatt
     1801 caacagccag taccgcatct ttacggcgtt tttccggcaa cagctcccgc agctttccca
     1861 tcgcctcatc tgccgactga gacacagagc agtagttttc tggtgtccgt tcagcgtcag
     1921 cattcggcgt ctcacgctcc ctgtagcagt gctgcaaagc gctggccaca ctgcccattc
     1981 ccgtcagctt tttgcagcgc attatcgcgt aattcgccat gcttaccccc tcgttctggc
     2041 gt
//
