LOCUS       CP100023                3906 bp    DNA     circular BCT 21-JUL-2022
DEFINITION  Escherichia coli strain EC602A1 plasmid p602A1-3K, complete
            sequence.
ACCESSION   CP100023
VERSION     CP100023.1
DBLINK      BioProject: PRJNA827955
            BioSample: SAMN27625687
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Tang,B., Chang,J., Yang,H., Wu,J. and Li,J.
  TITLE     A 12-year investigation on the antimicrobial resistance of
            Escherichia coli from diseased swine
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Tang,B., Chang,J., Wu,J., Yang,H., Lin,J., Guan,C., Jiang,H.,
            Chen,H. and Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
REFERENCE   3  (bases 1 to 3906)
  AUTHORS   Tang,B., Chang,J., Yang,H., Wu,J. and Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. JAN-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/29/2022 22:33:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,933
            CDSs (total)                      :: 5,804
            Genes (coding)                    :: 5,484
            CDSs (with protein)               :: 5,484
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 98
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 99 of 320
            Pseudo Genes (incomplete)         :: 227 of 320
            Pseudo Genes (internal stop)      :: 56 of 320
            Pseudo Genes (multiple problems)  :: 55 of 320
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC602A1"
                     /isolation_source="gut"
                     /db_xref="taxon:562"
                     /plasmid="p602A1-3K"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2018-03"
     gene            complement(join(3425..3906,1..538))
                     /locus_tag="MZH69_29645"
     CDS             complement(join(3425..3906,1..538))
                     /locus_tag="MZH69_29645"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UTU27357.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDSSVKA
                     LKYAAAIERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     PECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSENQSDKGKKS
                     AAVRWTANDDKRRRALDMYILGASTEDIAVAVGVSSRTIRRWMDNSGEWLTKKQIIKF"
                     /name="replication initiation protein"
     gene            complement(996..2546)
                     /locus_tag="MZH69_29650"
     CDS             complement(996..2546)
                     /locus_tag="MZH69_29650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="UTU27358.1"
                     /translation="MASYHCTVKTGAKGSALKHADYISRTGEYENYKSREDLEFSSSGN
                     MPSWAKKNPSELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGDRH
                     AYQYAIHNPPGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKLPERGGAKKARF
                     GETQQERKSALVELRSRWADVQNEHLARHGHESRVDHRSLKEQGINRTPEVHLGPVQAA
                     SLNGEQIVAIQERRNAERELKTARDAANAIQQEQEQKQKIKAVEPVRSARSPELLLQYR
                     KVMKTVIQGEARLARLGDANPNALKEHKLLQNAKAKKDSLSEWSRRIYEGARYLDKLGR
                     NVVSAQRELRELQEQRNALNGIRGLFRGADKREIDARILEQKSVLETAEHERNEFRNKL
                     QQAESEWDKENAAFKRTEGYKYVGDLDRYREREILAAASLENTRQKVAEEVSVARSQML
                     SLEPELSISGDEKAQMRHELLAEMAQERQQQEEKALRIQRSWAREASRSNERDQDMER"
                     /name="MobA/MobL family protein"
     gene            2718..3164
                     /locus_tag="MZH69_29655"
     CDS             2718..3164
                     /locus_tag="MZH69_29655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="UTU27356.1"
                     /translation="MTNREEWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKTL
                     SALIRAEKTAEKAAAAKARVTAIIAAERKAAARAERKARDHELYKAAGLMIVAGLVDSK
                     TGKPKFSAAELVGALAGIAELPRNHPKWQEWERRGKELLTKDSA"
                     /name="conjugal transfer protein TraD"
     Typing          complement(2..538)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.733"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          complement(3685..3901)
                     /name="rep_cluster_2344"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.165"
                     /coverage="84.0"
                     /evalue="2.37e-103"
     Typing          complement(1000..2546)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.734"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acagatggca gtttcgcccc atcccgtaat gtttatcgat gctacggcgc gctgaggcgc
       61 tcaaatcgag ataatcagcc agttcatcga gggtataggc ttcctcgcgc cactccatca
      121 cctgccattc caggtggaac ggatttttgc aaatcagtcc gctgtagttc acatctgcac
      181 aaagtttctc acacagcgaa cgttcaatcg cagcggcgta tttcagcgcc ttaaccgagc
      241 tgtccggcgc ggttctcacg gcgatattga gcgcgtagag caggtgagca tgaccgttca
      301 caggattttt tacggtgatg ttcggcgcgg gagcgttccg gtcgctccag tcaatcgctg
      361 ccccctcgcg gtcaacgtca aaaaccagcc agaactgcgc atgaggctgg ttctgctgta
      421 tgtaccgcgc aagaagcgcg cgccctttgc cggagatgcg aagaccaaaa gcgagatcat
      481 ccgtgtgata gggacggtgc ggtaaatttt cgtcgaagta ttgaagcgcg gcgctcatgt
      541 gttacgattc tctccgggtt atgcgaccta cacgccctct gtatttcatg ttgaatcttg
      601 ccggatctgg acatgatttc aggggcgttt gtttttcagt cgcttacact acacccaacg
      661 gcaaatcctg tcgatccttc ccgaaaaact gatccattta ccataactcc tgttgtacgt
      721 accccgtaaa acgccctcag tcgcttcctg acgcaaaaaa cgcaaaaaga caggaaaaca
      781 ccaggccaaa ccagaaaaac gcgtcagaat cgttttcaga gcgttgcata actatgcacg
      841 aaagcgatgc gcgatctgtt tcgagcgttc agaagggcga acgggtcaaa acagcaaact
      901 ttctcatcag aaaacggtga acgggacgcg gaattttttc gcgttccgtg aaacgttggc
      961 ggggttcggg gcgcagtccc gatattgcta cattttcaac gctccatatc ttgatcacgt
     1021 tcgttactac gtgatgcctc cctggcccag gatcgctgta tacgcagtgc cttttcctcc
     1081 tgttgctgcc gttcctgcgc catttctgcc agtaactcat gtcgcatttg agctttttca
     1141 tctccagata tactgagttc aggctccaga gataacatct ggctacgggc aacggatact
     1201 tcttcagcaa ccttttgcct ggtattctct aaagatgccg cggccagaat ctcacgttct
     1261 ctgtacctgt ccagatcccc aacgtactta tatccctcgg tacgcttaaa agcagcgttt
     1321 tctttgtccc actcggattc agcttgctgt agcttgttcc tgaattcatt cctttcatgt
     1381 tcagcggttt ccagcacact tttttgctcc agaatacgcg catcaatctc ccttttgtca
     1441 gccccccgaa acaggccacg aatcccattc aaagcattgc gttgttcctg tagctcccgc
     1501 agctcccttt gtgcggaaac gacattacgc cccagtttgt ctaaataccg tgctccttca
     1561 tatattcgcc tgctccattc ggaaagagag tcttttttag ctttcgcatt ctgaagcagc
     1621 ttatgttctt tgagcgcatt gggattggca tcccctaaac gagcaagcct tgcctctccc
     1681 tgaatgaccg ttttcataac cttgcggtac tgaagcaata actccggtga acgggctgaa
     1741 cgaacaggct caactgcctt gattttttgc ttttgctcct gttcctgctg aatggcattt
     1801 gcggcatctc tggcggtctt gagttcccgc tcggcattcc tccgttcctg gattgcaaca
     1861 atctgctcac cattcagact ggcggcctga acggggccaa gatgaacttc cggcgtgcga
     1921 ttgataccct gttctttcag cgaacgatga tctacgcgcg actcgtgacc gtgacgcgca
     1981 aggtgctcat tctgcacatc cgcccagcgg gaacgtaatt caacaagggc agattttcgc
     2041 tcttgttgtg tttcaccaaa gcgtgccttt tttgcgcccc ctctctccgg taatttgctg
     2101 ttagcacgtt taaaaaactg ctgcggatcg cgttcaatac catcattaat ccgctcgcaa
     2161 aacatgatat gcgcgtgtgg ttgttccttt ccgtcgattg cacctggcgg attgtggatc
     2221 gcgtactggt aggcatggcg atcgccaagt tccttttgca caaaatcttc aacaagttct
     2281 atccgttgtt cgcgtgtcag ttcacgcggt aacgcgatct caatttcacg gtatgccgtc
     2341 ccgttttttc gttcaaattc atctgcggct ttccatagct cggagggatt tttcttcgcc
     2401 catgatggca tgttaccgga tgacgaaaac tccagatcct cccgcgattt atagttctcg
     2461 tattcgccgg ttcttgaaat gtaatcagca tgttttaagg cactgccttt tgcaccagtt
     2521 ttaacggtgc aatggtaact agccatctgc cttttagccc tccgcaggag cgcacagttt
     2581 ctcgacgaag gagagaaacg tctaagtgcg catccctgaa taaatcaggg atgattttac
     2641 gttctcacac gcaggcagac aaggtgatat tatgcacaat aacaactcac attaacggca
     2701 aaagtgcaga ggtaaggatg acaaatcgtg aagagtggtt aagtgcaaaa attgcctaca
     2761 tcaacggact gaaatcaccg tcagagcagc agagattact tgtgttactg gcagagaaaa
     2821 aaaaccgaac aacgacggat gaaaaaacgt tgtccgcact gatacgggca gaaaaaaccg
     2881 cagagaaagc agccgcagca aaagccaggg taacggcaat catagcggca gaacggaagg
     2941 cagccgcaag ggcggaacga aaggcgcggg atcatgaact ctacaaggcc gcaggcctca
     3001 tgattgtggc aggactggtg gacagcaaaa ccggaaaacc aaagtttagt gctgccgaac
     3061 tggtaggcgc actcgcagga atcgcagagc taccgcgcaa ccacccaaaa tggcaggaat
     3121 gggaaagacg cgggaaggag ctactaacca aagacagtgc gtaagagcct ccgttcacgt
     3181 tccgtttccc tggtgcttcc tcgctcattc gctcgctacg ctcggtcatg agactgcggc
     3241 gagcacaacc acctcaatca tgcggtaagc cgcaccattg cagggctgag aggagaaaac
     3301 cgccggaaag cccgctgctc aaaaagacgc cgaaaggcgc tgttatctga tataggcttg
     3361 ttcatgtccg cgaatttaac cacccattaa caaaaacagg gggtaattgt catttttctg
     3421 ttaattaaaa tttaattatt tgtttcttgg ttaaccattc tccagaatta tccatccagc
     3481 gtcgaatagt ccggctgcta acaccaaccg ccactgcaat atcttccgtt gacgctccca
     3541 agatatacat atcaagcgcc ctacgacgtt tgtcgtcgtt agcagtccac cgaacagcgg
     3601 cgcttttttt ccctttgtcc gactgatttt ctgacgaatt ggccttcccg cccttgcgac
     3661 cgcgtgctgc ctgaatttct ggcgtgtgcg tatcagccac atactgtgcg aaagtttccg
     3721 gcgtgaagtt cctgtgcgtg tatttcgcaa tgctcttgcc aatagcccga cattcaggcg
     3781 gtgaaagcgg aacgggaagc gatgcgttgt acatttcgac acgctgaatc acggcatcaa
     3841 gccactgtga gaatgccggc cagccctgac gaatcgccct gtaagcccat ttgcgcgtca
     3901 tttcga
//
