LOCUS       CP100022                4677 bp    DNA     circular BCT 21-JUL-2022
DEFINITION  Escherichia coli strain EC602A1 plasmid p602A1-4K, complete
            sequence.
ACCESSION   CP100022
VERSION     CP100022.1
DBLINK      BioProject: PRJNA827955
            BioSample: SAMN27625687
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Tang,B., Chang,J., Yang,H., Wu,J. and Li,J.
  TITLE     A 12-year investigation on the antimicrobial resistance of
            Escherichia coli from diseased swine
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4677)
  AUTHORS   Tang,B., Chang,J., Wu,J., Yang,H., Lin,J., Guan,C., Jiang,H.,
            Chen,H. and Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
REFERENCE   3  (bases 1 to 4677)
  AUTHORS   Tang,B., Chang,J., Yang,H., Wu,J. and Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. JAN-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/29/2022 22:33:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,933
            CDSs (total)                      :: 5,804
            Genes (coding)                    :: 5,484
            CDSs (with protein)               :: 5,484
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 98
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 99 of 320
            Pseudo Genes (incomplete)         :: 227 of 320
            Pseudo Genes (internal stop)      :: 56 of 320
            Pseudo Genes (multiple problems)  :: 55 of 320
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC602A1"
                     /isolation_source="gut"
                     /db_xref="taxon:562"
                     /plasmid="p602A1-4K"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2018-03"
     gene            1108..2406
                     /locus_tag="MZH69_29625"
     CDS             1108..2406
                     /locus_tag="MZH69_29625"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="UTU27355.1"
                     /translation="MMVNFSLSNAFSFMEAQNMSMLAVSSSKDDINLANVVSVRDERIL
                     KSALIFGANASGKSNLMKTISLMKDIVLHSVQAIESGITKNVVPFLLDEAGPTKPSEME
                     MTFIADGIKYRYGLSLFQGKIKEEWLYYTPKTRETILFERDGQLIEINKAAFSESNLFI
                     KDGVIQKTREDVPFVSVVAGFNGEHSKRVVNWFSNVVFISGSNEGMFTGITMKLINESE
                     DFKAWLKKILPAFQINDIYLDEVAPEFPFGNIKTDDDNLKNLFSSIQNFAKNQKVFSLR
                     VVKKTANGEISVPIDFESEGTKKIVYLLGPIYNAMINNNLLLVDEFDSKFHTLLSKFLF
                     RIFHAQSEGFSQVIAAVQDVNLMNTDCFRRDQIWFVDKDVKSGSSHLYSLVEYKEKQRS
                     LKPSYGNDYLSGAFDAIPLFDNLNEFEKLMPEG"
                     /name="ATP-binding protein"
     gene            2411..2848
                     /locus_tag="MZH69_29630"
     CDS             2411..2848
                     /locus_tag="MZH69_29630"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UTU27352.1"
                     /translation="MPKRGKSIRSSSRNEETKPSKSTAKTTDRFISISFKHFHNVDDVG
                     QSINTWKDDGSLPDLIEKLVYLTANGITKVISDGVFTNYTRFPESAVNDFRCPDEISSD
                     EDWGVIKNIGGQKRRVAGFLRDNVFYIVFFDRDHRFWKSQK"
                     /name="hypothetical protein"
     gene            complement(3083..3196)
                     /locus_tag="MZH69_29635"
     CDS             complement(3083..3196)
                     /locus_tag="MZH69_29635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UTU27353.1"
                     /translation="MHEPPVQSDRCAFSGNYQLESNPERHDKTPMAAATGN"
                     /name="replication initiation protein"
     gene            complement(3833..4027)
                     /locus_tag="MZH69_29640"
     CDS             complement(3833..4027)
                     /locus_tag="MZH69_29640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UTU27354.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQIRFEAESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          3676..3760
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.837"
                     /coverage="100.0"
                     /evalue="1.41e-35"
     Typing          4064..4162
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.0"
                     /coverage="100.0"
                     /evalue="1.35e-41"
     Typing          4287..4542
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.86"
                     /coverage="89.0"
                     /evalue="1.25e-96"
     Typing          complement(3125..3662)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.993"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gcctgacgca tttttccggt ttaccccggg gaaaacatct ctttttgcgg tgtttgcggc
       61 agaatcgcgt tcagcgcgtt ttagcggtgc gcgcaatcgg atgttatggt aaatccagct
      121 gattggcagg tataaaaaag ccgtccgtag acggcctagt aagttacttc gtctttgtca
      181 tcactaccaa ttcccatcgg atgtcacctg ggacattatg ctgcctatga ttttgcggtg
      241 gcaaaggatc atctttattg cacgtcactt catcaccaca gttcgtgcag cggtaaatgc
      301 cggaagccgg acaagtattt cccggcttat agtaataccg ccatacactc ccttcaggct
      361 cggttgtttt tgttaaaaat ttcgctgtgc tatctacata aaaggccatg ttgctttcct
      421 tacataaact aagttaggga ccgaagcttg catgcttcgg tggttattgt tttttctttt
      481 cccgattaat tgggatctta aacattcccc cgttaggacg ggttatccgt ttgcccttta
      541 ccgttatgta agggcgaaaa atgtatgcct gaggctcgtc cgtctgcatg ttgcatttcc
      601 tattttcgcc acatgaatgt tgatttttgc tatcaaaatt gtactgtagc aaccaacaga
      661 tcttgtggct ggctttatca aagcacttga ggtggtactc aagcgccgca tttgcgtatg
      721 gtttgttacg ggttagttct ggtggtacag gctaacccac ttacttcaac aatttttgct
      781 tctcctcatt catgtttcac tccctacgta tttttttaca gtgtcagcgc cactgagcat
      841 aaacgcgaca aaaaggaacc atccccaccc agataccgag tgaaaagcca gcactgctga
      901 gctaatcact gaggcaaatg ggaccatttg gtatagaaaa aacttcatat acacctcttt
      961 ttaagctggt cgttaactta tctactcata atgtgctgtc tattggaccc ttttgttgtt
     1021 ttagtacggg gttatgctat tcttaatcta gaattttgtc aatattgtgc aatttttgag
     1081 tttaagggtg ttgttagggg tgttcttatg atggttaact ttagtctttc aaacgcattt
     1141 tccttcatgg aagctcaaaa tatgagcatg ttggcggtgt cgtcatcgaa agacgatata
     1201 aacttggcta atgtagtgtc agtaagggat gagcggatac tgaagtctgc tttgattttc
     1261 ggagctaacg ctagcggaaa atctaatctc atgaagacaa tatctttaat gaaagatatt
     1321 gtcctgcatt ctgtgcaagc tatcgaaagt ggcattacga agaatgtagt ccctttttta
     1381 cttgatgagg cggggcctac taaaccatct gaaatggaaa tgacttttat tgcggatggg
     1441 attaaataca gatacggatt atctttattt caagggaaga taaaagaaga gtggttgtac
     1501 tacacgccaa aaacaaggga gactattctc tttgagcgag atggtcaact tattgaaata
     1561 aataaagcag catttagcga gtcaaatctt tttataaagg atggggtcat tcagaagaca
     1621 agagaagatg ttccctttgt ttctgttgtt gctggattta atggggagca ctcaaagcgt
     1681 gttgtgaatt ggtttagtaa tgtggtattt atttctggtt ctaacgaagg aatgtttact
     1741 ggaattacca tgaagcttat aaatgaatct gaagatttta aagcttggct aaaaaaaata
     1801 ttaccagcct ttcagattaa tgatatttat cttgatgaag ttgcgcctga attcccgttt
     1861 ggcaacataa aaacagatga tgataattta aaaaatttat tcagctcgat tcagaatttt
     1921 gcaaaaaatc aaaaagtatt ttctcttaga gtcgtgaaaa aaactgctaa tggggagatt
     1981 tcggttccca ttgattttga atcagagggg accaaaaaga tagtctatct ccttgggcct
     2041 atttataacg ccatgattaa caataactta ttattggttg atgagttcga ttcaaagttc
     2101 catacccttt tatctaagtt tttatttagg atatttcatg cgcaaagtga agggtttagc
     2161 caagtaatcg ctgcagttca ggatgttaat ttaatgaata ctgattgctt tagacgagac
     2221 caaatttggt ttgttgacaa ggatgtaaag tcagggagta gccatcttta ttctttggtt
     2281 gagtacaaag agaaacaaag atcattgaag ccatcatatg ggaatgatta tttatcaggt
     2341 gcttttgatg caatcccgtt atttgataat cttaatgaat ttgaaaagct aatgcctgag
     2401 ggatgattat atgccaaaga gaggcaaaag cattcgaagc tcttcgagga atgaggaaac
     2461 aaaaccttca aagagcacag cgaaaacgac tgatagattt atatctatta gttttaagca
     2521 ttttcataac gtcgatgatg ttgggcagtc cataaataca tggaaagatg atggtagcct
     2581 acctgatctc attgaaaaat tggtttatct aacggcgaat ggaatcacta aagtgatttc
     2641 tgacggtgtg tttactaact acactcgatt tcctgagtca gctgtaaacg attttagatg
     2701 cccagatgag atatcctctg atgaggattg gggggttata aaaaacattg gcgggcagaa
     2761 aagaagagtc gccggatttc tgagagataa tgtgttctat atcgtgtttt ttgatagaga
     2821 tcatcgcttc tggaagagtc aaaaatagat gagatacagt tgtattgtat gttaagaaaa
     2881 gatcttcttg agattgtgat gctctcgtcg taatctcttg ctctgtaaac gaaaaaaccg
     2941 ccttggcggg cggtttttcg acggttctaa gagttggcgc tctgtgaacc aaggtaactg
     3001 gcttatgagg agcgcagtaa ccaaatctgt tctttcagtt tagccttaac aggcgcataa
     3061 cttcaagact aactcctcta aatcagttac cagtggctgc tgccattggc gttttgtcgt
     3121 gtctttccgg gttggactca agttgatagt taccggaaaa ggcgcagcgg tcggactgaa
     3181 cggggggttc gtgcatacag tccagcttgg agcgaactgc ctacccggaa ctgagtgtca
     3241 ggcgtggaat gagacaaacg cggccataac agcggagtga caccggtaaa ccgaatggca
     3301 ggaacaggag agcgcacgag ggagtcacca gggggaaacg cctggtatct ttaagtcctg
     3361 tcgggtttcg ccacctctga cttgagcgtc ggtttttgtg atgctcgtca ggggggcgga
     3421 gcctatggaa aaacgccagc ggcgcggcct tcctcctgtt cctggtcttt tgctcacatg
     3481 ttctttccgg ccttatcccc tgattctgtg gataactgtg ttaccgtttt tgtgtgagtc
     3541 agtaccgctc gccgcagtcg aacgaccgag cgtagcgagt cagtgagcga ggaagcggaa
     3601 aagcgcctgg acatgcattt tctccttacg catctgtgcg gcatttcaca cccggcatgg
     3661 cgtacttttc atacaatccg cactgatgcc gcatggttaa gccagtatac actccgctat
     3721 cgctacgtga ctggttcagg gcttcgcccc gaaaccccct gacgcgccct gacgggcttg
     3781 tctgctgacg aaaacgccca cgggaaagcc gtgaccgcct ccgtcagctt cgttatgttc
     3841 ctgtttcact ttcggcctca aagcgaatct ggatgctgtt ctggagttct tctgcgagtt
     3901 cgtgcagtcg ttcgcacatg gccgcctgtt cgtcggcatc cagagcatcc agtttttcaa
     3961 gcagtgtcag gctctggctt ttgataaatc gcgccatatt cagcgcagtt tgttgttgtt
     4021 tgttcacctt ccgttgttct ccgtcagttc cgggtgtcgg ggggaccctg agcaggtggt
     4081 aattgtaaga cgttgcgggc gacggtatta caattgcaca tcctgtcctg tttctttagg
     4141 acgtatcatg gcacagcata tcaactgata aataatccct gtgcagtgca gccgctgttg
     4201 cataaaattt cgctctgcgt cgagtgtttc ttaatcaata attccgctga tactccggaa
     4261 aaacgcgagt ctgttctcat tcctgaaatg cccacacccc acgcaaaaac aagtttttgc
     4321 tgatttttct ttataaatag agagttatga caaattagtt cttcttgctc tctttgtgat
     4381 atttaaaaaa gcggtgttgg cgcagctaca acaccgcgcc gacaccgctt tttaggggtg
     4441 gtactgacta ttttcataaa aaacatcatc ttatattagg ggtgctgcta gcggcgcggt
     4501 gtgttttttt acaggacgcc cctgggggcg ctgccagggg tgtctgttca gatatgtagc
     4561 aatgagtcag ggattcacga actgaaaatg tccgaaaatg tgtctcatgg gcactgtatg
     4621 cgcccaaacc gatcgtcatt cactttcatg catagctatg cagtgagctg aaagcga
//
