LOCUS       CP100021                5060 bp    DNA     circular BCT 21-JUL-2022
DEFINITION  Escherichia coli strain EC602A1 plasmid p602A1-5K2, complete
            sequence.
ACCESSION   CP100021
VERSION     CP100021.1
DBLINK      BioProject: PRJNA827955
            BioSample: SAMN27625687
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5060)
  AUTHORS   Tang,B., Chang,J., Yang,H., Wu,J. and Li,J.
  TITLE     A 12-year investigation on the antimicrobial resistance of
            Escherichia coli from diseased swine
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5060)
  AUTHORS   Tang,B., Chang,J., Wu,J., Yang,H., Lin,J., Guan,C., Jiang,H.,
            Chen,H. and Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
REFERENCE   3  (bases 1 to 5060)
  AUTHORS   Tang,B., Chang,J., Yang,H., Wu,J. and Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. JAN-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/29/2022 22:33:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,933
            CDSs (total)                      :: 5,804
            Genes (coding)                    :: 5,484
            CDSs (with protein)               :: 5,484
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 98
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 99 of 320
            Pseudo Genes (incomplete)         :: 227 of 320
            Pseudo Genes (internal stop)      :: 56 of 320
            Pseudo Genes (multiple problems)  :: 55 of 320
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC602A1"
                     /isolation_source="gut"
                     /db_xref="taxon:562"
                     /plasmid="p602A1-5K2"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2018-03"
     gene            complement(join(5058..5060,1..774))
                     /locus_tag="MZH69_29590"
                     /pseudo=""
     CDS             complement(join(5058..5060,1..774))
                     /locus_tag="MZH69_29590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(764..1087)
                     /locus_tag="MZH69_29595"
     CDS             complement(764..1087)
                     /locus_tag="MZH69_29595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UTU27351.1"
                     /translation="MLTIRVTDEEHARLLERCEGKRLAKWMRRVCLGEPVARTGKLPTL
                     APPLLRHLAAIGNNLNQTARKVNSGQWSSIDRVHVVAALMAIEGELRQLRQAVREQGVR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1151..1342
                     /locus_tag="MZH69_29600"
     CDS             1151..1342
                     /locus_tag="MZH69_29600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UTU27350.1"
                     /translation="MTKQEKTALNMARFIRNQTLTLLEKLNDLDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2658..2891)
                     /locus_tag="MZH69_29605"
     CDS             complement(2658..2891)
                     /locus_tag="MZH69_29605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UTU27346.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHEVTQRNNA
                     ILNERVVILSQQVQRLAGQVDRLSRLFIANNS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2898..3284)
                     /locus_tag="MZH69_29610"
     CDS             complement(2898..3284)
                     /locus_tag="MZH69_29610"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UTU27347.1"
                     /translation="MKKQLDTLSTTVSMQFSTTEAELSRQQKKLLWRVIKGRILFPALT
                     ALSVTGGIFLGCWGLMEWQESKIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLV
                     LPDGVKGENTWTVGDKNAVRLVRE"
                     /name="hypothetical protein"
     gene            3488..3676
                     /locus_tag="MZH69_29615"
     CDS             3488..3676
                     /locus_tag="MZH69_29615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UTU27348.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVTTSIL
                     KRRGDTAALTLLQDTGV"
                     /name="hypothetical protein"
     gene            3686..4885
                     /locus_tag="MZH69_29620"
     CDS             3686..4885
                     /locus_tag="MZH69_29620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="UTU27349.1"
                     /translation="MLSGFTPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKMLACGT
                     RILGVKEFGCDNPDCQHVKYLTNSCGSRACPSCGKKATDLWTATQLNRLPDCDWVHLVF
                     TLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGLEPGIFCAIHTYGRRLNWHPHVH
                     VSVTCGGLNKHGQWKKLSFLKDAMRSRWMWNMRQLLLKAWSEGMAMPESLSHITTESQW
                     RSLVLKSGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLSFRYLD
                     HKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRALGMDKP
                     EPVAKVCYAQMVKQFLSRDPFECVLCGCRMVYRRAIAGLNVSGLKKNARDISLLRYMPA
                     "
                     /name="IS91 family transposase"
     Typing          complement(1424..1504)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.585"
                     /coverage="93.0"
                     /evalue="4.04e-16"
     Typing          complement(2..774)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.062"
                     /coverage="100.0"
                     /evalue="1.1e-167"
     Typing          complement(2576..3372)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="87.454"
                     /coverage="100.0"
                     /evalue="1.45e-120"
ORIGIN
        1 tcgctgttca gcatgttctc ggtagaagcg gctcgctctt tcagtttctt ctcgaagtct
       61 gttcccattt gtaaaagatt gctcatacag cgcccccttt agcctgagat tccgcccccc
      121 ctccgggtcg gcaatactga tgctgctttt ggttgtcctc accacctcaa aacctgccgc
      181 tgtaagcgcc tctgtgacat cctgacgcgt ttttattgct ccggcatggt aaagagcctc
      241 aagaccccgt gtaatcgctt ctgcggcctc ctgtttcgct tttggcagat tatttggcgt
      301 gacaagcgtc cgtctgttct ccggtgcgtt cgggtcacgc agcccgtaat gatgattaac
      361 cagcgtctgc caggcattta tcctcggcct gtccgcacgg tcgtagtacg gctggaggcg
      421 cttaccgctc tgtagctcca tgttcgggat gacaaaattc agttcgagcc gccccttatc
      481 ctggtgttcc acccagagaa tgctgtactg gtctttttcg agaccgggca taagaacacg
      541 ctcaaagctc gtcatcactc tttcgcgttc acccggtggt agctccttct ccgcgaacga
      601 cagaacgcct gacgtgtatt tcttcgaaaa tggcgtggaa tcaatgagtt cccgcacttc
      661 ttccgggtta ccccggagca ccgttgcgcc ttcgcggtta cgctcccgcc ccagcaggta
      721 atcgaccggg ccacttccac cgcctttccc cctggcatga aatttaacta tcatcccgta
      781 ccccctgttc cctgacagcc tgccgcaact ggcgcaattc accctcgatg gccatcagtg
      841 cggccaccac atgaacccgg tcaatcgaag accactggcc gctgttcacc ttccgggctg
      901 tctggttcag gttgttcccg atggcagcca gatgccgcag caatggcggt gccagtgtcg
      961 gcagttttcc tgtgcgtgca accggctccc ccagacagac ccgccgcatc catttcgcca
     1021 gtcgtttacc ctcgcagcgt tccagtaacc gggcgtgctc ctcatcggta acccgtatcg
     1081 tgagcatctt ctcgcgtttc atcggtatca ttccccctaa caaatccccc atacaaggag
     1141 ccttaagacc atgaccaaac aggaaaaaac agcccttaac atggctcgct ttatcagaaa
     1201 ccagacgcta accctgctgg aaaaactcaa cgatctggac gcggacgaac aggcagacat
     1261 ctgcgaatca cttcacgacc acgccgacga actctaccgc agttgcctcg cacgcttcgg
     1321 cgatgacggt gaaagcaact gaaacatgca gctcccggag acgttcacag cttgcctgtg
     1381 agcggatgcc gggagcagac aagcccgcca gggcgcgtca gtgcgggtgt cggggcgcag
     1441 ccctgaccca gttcacagag cgatagcgat gtgtatactg gctttcctca ttttgcatcg
     1501 gtgcagagtg gatgggaagt gcaccatgtc aggagtaaaa agccgcacag atgcgtaaaa
     1561 acagcgcgcc cgataaacaa cacacacgcg gacactggca caggcgcgta agtgcacaat
     1621 gcttgcctct gtgactcttc cgcttcctcg cccactcgct cactacgttc ggtcgctggg
     1681 gctgcggcga gcggtgggat cactctcgtt gtgatgcaga tccgtttttt ttttgcactg
     1741 gagttatcag gagtagcggt gcacatggga tcgtgcgata tgctggatgg tggaaggtcg
     1801 gagggaacgg caacaactca cccctcccag tgaagggacg ggtagtttgc cgctctcgat
     1861 atgccggacg gagccaaggc catcgatcac ggggcgcgtc agtggaagaa cccgcgcctg
     1921 cgtgacgtat gcgtcttcgc cgcaccatct cacccgtcac gccaccacat accgggaact
     1981 gccgccaagg tctaactcaa ccaatctagc cgcgctacat acggtttttg tggcaactca
     2041 tcaggtgtcc gctgtcgatg aaactctctg cgccggtagc ggaactctca cgcgtgcggt
     2101 cgccgtcgtc acagctcccc caaccgcaaa cgattctcgc aaatacgcaa gtatctgcaa
     2161 aaacaggttt ttttcggggt tcctgggaga ctgtgctgtt gaaaaactct tgaagaatac
     2221 agaaggctca tgcaaactga atgaactggt ttctgttgtg gatctcaaca gtttccacag
     2281 gaacattgag gaggtcagag acctcaatga acccgaaaca gctcgccttc ctggcgagct
     2341 gttttcgttc tacgcccacc gacgaaagat cgcaagtgca ataacgagat ttttcacgtc
     2401 tttacgctca aaaagtaaaa aaaccatgtt ttcccctggc tgaatcccca aaaacgcgtg
     2461 aaatgcgttt tcaggatgtt gcatagctat gcacaaatac ggcacacgat ttgtttcgct
     2521 cgccccagcc gtcgaacggg tcaaaatggc aagatttcgt cacttctccg gtcacatctc
     2581 cattgatggc ccactccgaa cccgtgccgc gcgttcctgt acctcatgtc tgtaccgggc
     2641 acgctcctgt tcatccctta gctgttgttc gcgataaata accgactcaa gcgatccact
     2701 tgcccggcta atctctgcac ctgctgactc aatatcacga cgcgctcgtt cagtatcgcg
     2761 ttgttccgtt gcgtaacctc gtgcatcttc tgcaattccg cgaaggcgct ctcccactcg
     2821 ttcagccgcc gcatatagtc ctgttgcaaa tgctctaagg cgttcagcaa atgcccttcc
     2881 agttccgtca tactcgttta ctccctgacc agcctcacgg cgttcttgtc gcccaccgtc
     2941 caggtgtttt cccctttcac cccgtccggc aacaccagaa atttcccgtt ctcgccattg
     3001 acgaacgtca ccccccaggt ttttgcctcg agtttcgcca gcgtgttttc ctgctctctg
     3061 atagtcagga tatttttcgc aatcttgctt tcctgccact ccatcagccc ccagcacccc
     3121 aggaaaatcc cgcccgtgac gctgagcgcg gtcagcgccg gaaacagtat ccgccctttg
     3181 atcacccgcc agagcagttt tttctgctgc cgggacagtt cggcctctgt ggtgctgaac
     3241 tgcatgctca cggtggtgct cagcgtatcc agttgctttt tcacggatgt tgtatgttcg
     3301 ctgatagcgt ctctgatttt ctgcctgttt aagctcaatg ccttgtctac agactcttcg
     3361 agcttcctga accacggcag cctggcggct gcaaattgaa aaccgccccg gttcgtcctg
     3421 ttgttgccgc acgccccgca gaatatgtat gctctgagga cctgttttat taaggactca
     3481 tccccttatg ttgacccgca aaagtatcga tacagttctg ctctctgttg gtgctgagaa
     3541 gctctcacaa cgagaatggg actggatgaa aatgctcaaa cccatggacc caccacctgc
     3601 gatggtgacg acctccatcc tgaagcgccg tggcgataca gcagccctta ccctgctaca
     3661 ggataccggg gtttaatcca tcctcatgtt gtccggtttt actccccgcc ctctgaaacg
     3721 tctgttcaca gccaaccagt gctggacgtc cttcctggat gcgggcggtc tgcgcgatat
     3781 cgaggttgaa gccgtcacca aaatgctggc ctgcggcaca cggatactgg gtgtaaaaga
     3841 gttcgggtgt gataacccgg actgtcagca cgtaaagtac ctgaccaact catgcggcag
     3901 ccgtgcctgc ccgtcctgcg gaaagaaggc cacagacctg tggacagcaa cacagctgaa
     3961 tcgtcttcct gactgcgact gggtacatct ggtcttcacc ctgccggaca cgctgtggcc
     4021 ggtgttcgaa agcaaccgct ggctgctgaa tgacgtgtgc cgtctggcgg tggagaatct
     4081 gctgtatgcc gcccgaaaac gggggctgga acccggtatc ttctgcgcca tccacacgta
     4141 tggccgtcgt ctcaactggc atccgcatgt acatgtgtct gtaacctgtg gaggtctgaa
     4201 taagcatggt cagtggaaaa agctgagctt cctgaaagac gcgatgcgtt cacggtggat
     4261 gtggaatatg cggcagctgc ttctgaaagc gtggtcagag gggatggcaa tgccggagtc
     4321 gttgtcacat atcacgacgg aatcacagtg gagaagcctg gtgctgaaat ccggcggaaa
     4381 atactggcat gtgtacatgt cgaagaagac agccggaggg cggaatacgg cacgctacct
     4441 gggtcgttat ctgaagaagc caccaatagc ggcttcccga ctggctcatt acaacggagg
     4501 ggcaagcctg agcttccgtt acctggacca caaaacggga gaaacggcga cggaaacgct
     4561 gacacagcgt gagctggtcg cgaggctgaa acagcacatc ccggagaagt tttttaagat
     4621 ggtgaggtac ttcgggttcc ttgccaaccg tgtgtgtgga gagaagctgc cgcaggtgta
     4681 ccgtgcactg gggatggata aaccggaacc agtggcgaaa gtgtgctatg cacaaatggt
     4741 gaaacagttc ctgagtcgtg acccgttcga atgcgtgctg tgtggctgcc ggatggtata
     4801 ccgccgggcc attgcggggc tgaatgtatc agggctgaag aaaaacgcgc gggatatcag
     4861 tctactgagg tatatgccgg cctgacacag gctaagtgtg cccgggaatc ggtgaaagta
     4921 taaaaaacag cctgttacgg tataaaaatc gaccatacag agagggaaac caccggagtg
     4981 gtgggaaaag cagaaaggct cacgtggcat aaggcgtgga caatggtatc gactggtttt
     5041 tcaatttcct atacgtgaac
//
