LOCUS       CP100020                5274 bp    DNA     circular BCT 21-JUL-2022
DEFINITION  Escherichia coli strain EC602A1 plasmid p602A1-5K1, complete
            sequence.
ACCESSION   CP100020
VERSION     CP100020.1
DBLINK      BioProject: PRJNA827955
            BioSample: SAMN27625687
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Tang,B., Chang,J., Yang,H., Wu,J. and Li,J.
  TITLE     A 12-year investigation on the antimicrobial resistance of
            Escherichia coli from diseased swine
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5274)
  AUTHORS   Tang,B., Chang,J., Wu,J., Yang,H., Lin,J., Guan,C., Jiang,H.,
            Chen,H. and Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
REFERENCE   3  (bases 1 to 5274)
  AUTHORS   Tang,B., Chang,J., Yang,H., Wu,J. and Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. JAN-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/29/2022 22:33:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,933
            CDSs (total)                      :: 5,804
            Genes (coding)                    :: 5,484
            CDSs (with protein)               :: 5,484
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 98
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 99 of 320
            Pseudo Genes (incomplete)         :: 227 of 320
            Pseudo Genes (internal stop)      :: 56 of 320
            Pseudo Genes (multiple problems)  :: 55 of 320
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC602A1"
                     /isolation_source="gut"
                     /db_xref="taxon:562"
                     /plasmid="p602A1-5K1"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2018-03"
     gene            join(5255..5274,1..172)
                     /locus_tag="MZH69_29555"
     CDS             join(5255..5274,1..172)
                     /locus_tag="MZH69_29555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UTU27342.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1687..2223
                     /locus_tag="MZH69_29560"
     CDS             1687..2223
                     /locus_tag="MZH69_29560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UTU27343.1"
                     /translation="MKDNKAVVVFTGKDLNIMRAEGGSGYWLAKAERLNDAKYLIAVRN
                     RRETWAVKDLEHGTGFLIAKITGCFKNPDYDDRNVITFEEYAEISVKKAWKMLTGGQRY
                     PVAYLTAEEAFSRIGITLEQLEWKKLQTSTLSQPIPSAEAPKKLSLNEAVERAKQDISN
                     ATDIDVSSITITINI"
                     /name="hypothetical protein"
     gene            2279..3103
                     /locus_tag="MZH69_29565"
     CDS             2279..3103
                     /locus_tag="MZH69_29565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UTU27344.1"
                     /translation="MTTTIYREFEFAACDSKWSVDGHLRRDVIEKKAIHIDFKGKEAIS
                     LMCGDHPMIVLMQAMQLKVITPEQYASLMSLYMDVEDFTFGMVVLDKHSGAILVGMYTP
                     PMIAIKSKQIVYDLGSTGGEFAAHCLYYAEKRLRKKKRHRGYSTSSVGCKITSAVKYAY
                     RRDRYSGGKVYKIIWKNNGIMYNNIPLSNNSVKNHYRRILKETLKIIHSRYEEKINMRQ
                     NAQLNTAAASLSQTGQPKTQSQPHNQAPSASADKSHQMTMSRAINYARLFSD"
                     /name="hypothetical protein"
     gene            complement(3430..3663)
                     /locus_tag="MZH69_29570"
     CDS             complement(3430..3663)
                     /locus_tag="MZH69_29570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UTU27340.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSERTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3670..4188)
                     /locus_tag="MZH69_29575"
     CDS             complement(3670..4188)
                     /locus_tag="MZH69_29575"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="UTU27341.1"
                     /translation="MSNLLRTGAEFEKKLKERAESTEKMLNDEFSRLGESVSEAVTSNE
                     TKIKGAIAQFTASTEESLKKHREGVKEAMMQHRKDMLKLAGSSGMRLLGIVFLLFTASG
                     GTLWYLGGRIQTNLEEIRVQEETLRKLNAKTWGVEFVQDGRRKFLVLPYGKSAEVTHYQ
                     GKDWIQLTE"
                     /name="MbeB family mobilization protein"
     gene            complement(4226..4657)
                     /locus_tag="MZH69_29580"
                     /pseudo=""
     CDS             complement(4226..4657)
                     /locus_tag="MZH69_29580"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4866..5189)
                     /locus_tag="MZH69_29585"
     CDS             complement(4866..5189)
                     /locus_tag="MZH69_29585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UTU27345.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQVVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(258..343)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="8.58e-38"
     Typing          358..892
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.05"
                     /coverage="99.0"
                     /evalue="8.1899999999999995e-171"
     Typing          complement(3327..4876)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.099"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgcccttaac atggcccgct ttatcagaag ccagacatta acgctgctgg agaaactcaa
       61 cgaactggac gcggatgaac aggcagacat ctgtgaatcg cttcacgacc acgctgatga
      121 gctttaccgc agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
      181 gctcccggag acggtcacag cttgcctgtg agcggatgcc gggtgcagac aagcccgtca
      241 gggcgcgtca gcaggtttta gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga
      301 tagcggagtg tatactggct taaccatgcg gcatcagtgc agattgtaag aaaagagcgc
      361 catatgcggt gtgaaatgcc gcacagatgc gtaaggagaa aatgcacgtt ccggcgctct
      421 tccgcttcct cgctcactga ctcgctacgc tcggtcgttc ggctgcggcg agcggtgtct
      481 gctcactcaa atgcggagat gcggttatcc acagaatcag gggataacac caaaagaaac
      541 atgtgagcaa aaaacgagaa cccggaaaag gccacgccgc tggcgttttt ccataggctc
      601 cgcccccctg acgagcatca caaaatcgac gctcaagtca gaggtggcga aacccgacag
      661 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccta
      721 cctttcggtt tactggtgtc actccgctgt tatggccgcg tttgtctcat tccacgcctg
      781 acactcggtt ccgggaaggc agttcgctcc aagctggact gtatgcacga accccccgtt
      841 cagtccgacc gctgcgtctt atccggtaac tatcgacttg agtccaaccc ggaaagacac
      901 gacaaaacgc cactggcagc ggccattggt aaacaggatg tgcgagagat gtatcgagag
      961 ttcttgaagt ggtgccctta gtgcggctac actggaagga cagatttggt ggcatgtctc
     1021 gcacaagact gttaccacgg ttagagaatc tgccaggatt aactaacctt cgaaaaacca
     1081 cctgccaggg tggttttttc gttttcagag ctagagatta cgcgtagaaa aaaaggatct
     1141 caagaagatc ctttttgtag acagcttata gatgattccc gtgaactgaa cgcgtatatc
     1201 caatagctat attaaacatt tcagcttgac ctggattcag atagcacgtt ctcagatcta
     1261 ctctcacaag caacccatta ccttcccgct cagcatagat accacgagac ctgcaaagat
     1321 ctattgattg ctctaccagc ttttcagtaa tgcgctgtct accgggagag cgcaggtcaa
     1381 gatagaaatt actaaccatc gtaccagctt cgctagaaag acgattgatg gtcgtttcaa
     1441 tacatgaatc tatttgctgc atagatatcg acatataacc cccaaaaaga ataacaccaa
     1501 aataatatac atataaaaaa ataaaatcaa ataaaagcaa cttaagcaca aaataagcaa
     1561 aaataagaaa gagataagca cagaatttgt agacttgctt ttatcgcata aataaaaccc
     1621 cacccctcat ttaatcgtat ataattaaaa tcatgacgat aaaaaactaa agcatgggat
     1681 ttctatatga aagataataa agcagttgtc gtatttacag gcaaagatct aaacataatg
     1741 agagctgagg gaggttcagg ttattggcta gcaaaagcag aacggcttaa tgacgccaaa
     1801 tatctcatcg cagtaagaaa ccggagagaa acatgggcgg tgaaagacct cgaacacgga
     1861 actggctttc tgattgcaaa gatcaccgga tgctttaaaa accctgatta cgacgacaga
     1921 aacgtaatta catttgagga atatgctgaa atttcagtga aaaaggcatg gaaaatgcta
     1981 actggcggtc agcgttatcc ggttgcttat ctgacagcag aggaagcttt ttcccgtata
     2041 gggataacgc tagagcagct ggagtggaaa aaactccaga cttcaacact atctcagcca
     2101 atcccctctg cagaagcgcc gaaaaaacta agcctcaacg aggcagttga aagagctaag
     2161 caagatataa gcaatgcaac tgatatcgat gtatcttcta ttacgataac tatcaatatc
     2221 tgattttttc gtatatctgg tatcttaacg atagtcgaaa aagaattaag ccattgatat
     2281 gacaacaaca atatacagag agtttgaatt tgctgcttgt gatagcaaat ggtcagttga
     2341 tggacatctt cgaagagatg ttattgaaaa aaaagcaata catatagatt tcaaaggaaa
     2401 agaagcaata tcccttatgt gtggtgatca tccgatgatc gtccttatgc aagctatgca
     2461 gctgaaagtc ataactccag agcagtacgc tagcttaatg agtctctaca tggatgtaga
     2521 agacttcacc ttcggcatgg tggtcctcga taaacactca ggtgctatat tagtaggtat
     2581 gtataccccc ccgatgattg cgataaaaag caaacaaata gtttacgatt taggttcaac
     2641 tggcggcgaa tttgcagcac attgtctcta ctatgcggaa aaaaggctta gaaaaaaaaa
     2701 gcgtcatcgt ggatactcta catcgtcagt gggatgtaag atcaccagcg ctgtcaaata
     2761 tgcatacaga cgagatcgct actccggtgg taaagtatac aaaatcattt ggaaaaataa
     2821 cggcatcatg tacaacaaca taccgttatc taacaactcg gtaaaaaacc attaccggcg
     2881 gattttaaaa gaaacattaa agatcattca tagcaggtac gaagagaaaa taaacatgag
     2941 acagaatgcc cagttaaata cagcggcagc ctcgctctct cagacgggtc agccgaaaac
     3001 acaaagtcag ccacataacc aagcacctag tgcgagtgct gataaaagcc atcagatgac
     3061 tatgtctcga gccattaact atgctcgcct tttttctgac tgatactgta cagctagatt
     3121 taccataatc ccttattgta cgcaccgcta aaacgcgttc atcgcgatca cggcagcaga
     3181 caggtaaaaa tggcaacaaa ccacccgaaa aactgccgcg atcgcgcctg ataaatttta
     3241 accgcatgaa tacctatgca accagatgat acaggccaca ttaacccctc ttaatccact
     3301 gaagctgcca tttttcatgg tttcaccatc ccagcgaagg accatccagc gtgcgttcct
     3361 gtatttccgg ctgacgctcc cgttccaggg ataacgcatg ttcgcgctcc tgtatcagcc
     3421 gttcctctct tatctccagt tctcgctgta taactggctc aagcgttctg tccgctcgct
     3481 caagtgctgc acctgctgac tcaactgcat gacccgctcg ttcagcatcg cgttgtcccg
     3541 ttgcgtaagc gaaaacatct tctgcaattc cacgaaggcg ctctcccatt cgttcagccg
     3601 ctgcatataa tcctgttgca gctgctctaa ggcgttcagc aaatgtgttt ccagctctgt
     3661 cattccgtat cactccgtca gttgtatcca gtccttcccc tgatagtgag tcacctctgc
     3721 tgatttcccg tacggaagga ccagaaattt ccttctcccg tcctgaacaa actccacgcc
     3781 ccatgtcttc gcgttcagtt tccgcaatgt ctcttcctgt accctgattt cttccaggtt
     3841 cgtctgtatc ctcccgccga gataccagag cgtcccgcca ctcgcggtaa acaggagaaa
     3901 gactatcccc agcaacctca tgcccgaact ccctgccagc tttaacatgt ccttccggtg
     3961 ctgcatcatc gcctctttca ccccttcccg gtgttttttc agcgattcct ccgtcgaggc
     4021 cgtaaactgg gctatagcgc ctttgatttt cgtctcgttt gaggtcacag cctcgcttac
     4081 agattcgccg agcctgctga attcgtcgtt cagcattttc tctgtagatt cggctctctc
     4141 tttcagcttt ttctcgaact ccgcgcccgt ccgtaaaaga ttgctcataa aacgctcctt
     4201 tcagtctgat attcctcccg ccgttcgggt ctgcaatgct gatactgctt cgcgtcaccc
     4261 tgaccacttc aagccccgcc tctgtgagcg cctgaatcac atcctgacgg ccttttatct
     4321 ccccggcatg gtaaagcacc tccaggcctc gtgtaacgct ctcagcaagc gcctgtttcg
     4381 tttccggcag gttatcaggg agggtcagcg ttctgcggtt ctccggggcg ttggggtcat
     4441 gcagcccgta atggtgattc accagtgtct gccaggcatc aattctcggc ctgtctgccc
     4501 ggtcgtagta cggctggagg cgttttccgc tctgtagctc catgttcgga atgacaaaat
     4561 taagctcaag ccgccccttg tcctggtgct ccacccacag gatgctgtac tgattttttt
     4621 cgagaccggg catcagtacg cgctcaaagc tctccatcac tttttcacgt cctcccggcg
     4681 gcagttcctt ctctgcgaac gacagaacac cggacgtgta tttcttcgca aatggcgtgg
     4741 catcaataag ctcccggact tcttccgggt taccctgaag taccgttgca ccttcccggt
     4801 tacgctccct ccccagaagg taatcaaccg gaccactgcc accacctttt ccccgggcat
     4861 gaaacttaac tatcatcccg cgctccctgt tccctgacga cctgccgtaa gctgcgcaac
     4921 tctctctcga tggccatcag tgcggccacc acctgaaccc ggtcgccgga agaccactgc
     4981 ccgctgttca ccttcctcgc cgtctgattc aggttattcc cgatggcagc cagctgacgc
     5041 agtaacggcg gtgccagtgt cggcagcttc ccggaacggg caaccggctc acccaggcag
     5101 acccgccgca tccataccgc cagttgttta ccctcacagc gttccagtaa ccgggcatgt
     5161 tcatcatcag taacccgtat cgtgagcatc ctctcgcgtt tcatcggtat cattaccccc
     5221 atgaacagaa atcccccctt acacggaggc atcagtgact aaacaggaaa aaac
//
