LOCUS       CP100019                8879 bp    DNA     circular BCT 21-JUL-2022
DEFINITION  Escherichia coli strain EC602A1 plasmid p602A1-8K, complete
            sequence.
ACCESSION   CP100019
VERSION     CP100019.1
DBLINK      BioProject: PRJNA827955
            BioSample: SAMN27625687
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8879)
  AUTHORS   Tang,B., Chang,J., Yang,H., Wu,J. and Li,J.
  TITLE     A 12-year investigation on the antimicrobial resistance of
            Escherichia coli from diseased swine
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8879)
  AUTHORS   Tang,B., Chang,J., Wu,J., Yang,H., Lin,J., Guan,C., Jiang,H.,
            Chen,H. and Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
REFERENCE   3  (bases 1 to 8879)
  AUTHORS   Tang,B., Chang,J., Yang,H., Wu,J. and Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. JAN-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/29/2022 22:33:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,933
            CDSs (total)                      :: 5,804
            Genes (coding)                    :: 5,484
            CDSs (with protein)               :: 5,484
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 98
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 99 of 320
            Pseudo Genes (incomplete)         :: 227 of 320
            Pseudo Genes (internal stop)      :: 56 of 320
            Pseudo Genes (multiple problems)  :: 55 of 320
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC602A1"
                     /isolation_source="gut"
                     /db_xref="taxon:562"
                     /plasmid="p602A1-8K"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2018-03"
     gene            join(8831..8879,1..101)
                     /locus_tag="MZH69_29500"
     CDS             join(8831..8879,1..101)
                     /locus_tag="MZH69_29500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UTU27333.1"
                     /translation="MHEPPVQPDRCALSGNCHLESNPERHVKTPLTAAIGNRLEELVLK
                     LCAG"
                     /name="replication initiation protein"
     gene            293..1132
                     /locus_tag="MZH69_29505"
     CDS             293..1132
                     /locus_tag="MZH69_29505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UTU27334.1"
                     /translation="MLTLIIFFVLLIAACFFCFAPPRRGYDRNEIIPYKIKLSINKYRL
                     YIYSSGKVRQYLLFLVILSLYYSIAEPFKSELIKNISYSLMAAFIFDTGLNFSKENITK
                     GVISTRWHNDLYSSFERMKAINKIYYPSNKEINTEGLSKAITSSLFNDDANSFAKRDFC
                     LMWDLSSEKYLSYKEIIIIKGDKLDAVCLRFINDDYKFLVNFNRDEEVFKYFPSIMQPS
                     LKTYRALSRLVNSIKDPSRFKFTTESLEMELLEYLELRNELFNDIEEVMGSYAQRAP"
                     /name="hypothetical protein"
     gene            complement(1168..1830)
                     /locus_tag="MZH69_29510"
     CDS             complement(1168..1830)
                     /locus_tag="MZH69_29510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010308590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UTU27335.1"
                     /translation="MPSNSIKASLSAARIGTYESILGGPAGVHRTEKALRLYMWNAQIS
                     AAFFMPLHVCEVITRNAVSEVLETVYGARWPWSIGFERSLPIPVHGYKPKDDLINGRRN
                     QPTTGKVIPELKFVFWQKMLTGRFDNRLWNNHILTAFPHAAAQGLNAAQLRQNLYNDLE
                     TVRKLRNRIAHHEPILNRNLEDDFATIKRIIAYRCQHSLEWMLKNQVLLPLLTLKPL"
                     /name="hypothetical protein"
     gene            2771..2965
                     /locus_tag="MZH69_29515"
     CDS             2771..2965
                     /locus_tag="MZH69_29515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UTU27336.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQVRFETESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3137..3325
                     /locus_tag="MZH69_29520"
     CDS             3137..3325
                     /locus_tag="MZH69_29520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UTU27337.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVTTSIL
                     KRRGDTAALTLLQDTGV"
                     /name="hypothetical protein"
     gene            3335..4534
                     /locus_tag="MZH69_29525"
     CDS             3335..4534
                     /locus_tag="MZH69_29525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="UTU27338.1"
                     /translation="MLSGFTPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKMLACGT
                     RILGVKEFGCDNPDCQHVKYLTNSCGSRACPSCGKKATDLWTATQLNRLPDCDWVHLVF
                     TLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGLEPGIFCAIHTYGRRLNWHPHVH
                     VSVTCGGLNKHGQWKKLSFLKDAMRSRWMWNMRQLLLKAWSEGMAMPESLSHITTESQW
                     RSLVLKSGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLSFRYLD
                     HKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRALGMDKP
                     EPVAKVCYAQMVKQFLSRDPFECVLCGCRMVYRRAIAGLNVSGLKKNARDISLLRYMPA
                     "
                     /name="IS91 family transposase"
     gene            complement(4707..4877)
                     /locus_tag="MZH69_29530"
                     /pseudo=""
     CDS             complement(4707..4877)
                     /locus_tag="MZH69_29530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /name="nuclease"
     gene            complement(4867..5190)
                     /locus_tag="MZH69_29535"
     CDS             complement(4867..5190)
                     /locus_tag="MZH69_29535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UTU27339.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5256..5447
                     /locus_tag="MZH69_29540"
     CDS             5256..5447
                     /locus_tag="MZH69_29540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UTU27330.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6649..6837
                     /locus_tag="MZH69_29545"
     CDS             6649..6837
                     /locus_tag="MZH69_29545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UTU27331.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVTTSIL
                     KRRGDTAALTLLQDTGV"
                     /name="hypothetical protein"
     gene            6847..8046
                     /locus_tag="MZH69_29550"
     CDS             6847..8046
                     /locus_tag="MZH69_29550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="UTU27332.1"
                     /translation="MLSGFTPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKMLACGT
                     RILGVKEFGCDNPDCQHVKYLTNSCGSRACPSCGKKATDLWTATQLNRLPDCDWVHLVF
                     TLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGLEPGIFCAIHTYGRRLNWHPHVH
                     VSVTCGGLNKHGQWKKLSFLKDAMRSRWMWNMRQLLLKAWSEGMAMPESLSHITTESQW
                     RSLVLKSGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLSFRYLD
                     HKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRALGMDKP
                     EPVAKVCYAQMVKQFLSRDPFECVLCGCRMVYRRAIAGLNVSGLKKNARDISLLRYMPA
                     "
                     /name="IS91 family transposase"
     Typing          complement(2237..2492)
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.86"
                     /coverage="89.0"
                     /evalue="1.25e-96"
     Typing          2668..2746
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.747"
                     /coverage="81.0"
                     /evalue="5.15e-16"
     Typing          complement(5526..5610)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.837"
                     /coverage="100.0"
                     /evalue="1.41e-35"
     Typing          complement(8246..8331)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.701"
                     /coverage="100.0"
                     /evalue="1.86e-34"
     Typing          complement(3..294)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.317"
                     /coverage="83.0"
                     /evalue="4.93e-148"
     Typing          5621..6178
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8344..8879
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.434"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4874..5223)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.436"
                     /coverage="99.0"
                     /evalue="1.46e-68"
ORIGIN
        1 gtcatcttga gtccaacccg gaaagacacg taaaaacgcc actgacagca gccattggta
       61 accgtttaga ggagttagtc ttgaagttat gcgccggtta aggctaaact gaaaggacag
      121 atttggtggc tgtgctcctc caagccagtt accttggttc aaagagttgc tgctctgaga
      181 accttcgaaa agccgccttg ctgggcggtt ttttcgttta cggacaagac taccttcagg
      241 atcgttagat ctcaagacaa tcatgcatag tgagcataca caggagccga acttgcttac
      301 actgatcatc ttttttgttt tattaattgc tgcatgtttc ttttgttttg ctccaccacg
      361 aagaggatat gacagaaatg agatcattcc atacaaaata aaactatcaa taaataaata
      421 tcgcctttac atatactcat cgggaaaggt aagacaatac cttttatttt tagttatttt
      481 atcactgtac tactccatag cggaaccatt taagtctgag ttaataaaaa acatatcata
      541 ctcactgatg gctgcattta tttttgatac agggcttaat tttagcaagg agaatataac
      601 taaaggggtg atttcaacaa ggtggcataa tgacctatat tcatcatttg agagaatgaa
      661 agcaattaat aaaatttact atccatccaa caaagagata aacacagaag gtctatccaa
      721 agccatcaca tcatctcttt tcaatgatga cgcgaactcg ttcgcaaaga gagatttttg
      781 cttgatgtgg gacttatctt ctgaaaaata tctttcctac aaagaaataa taataataaa
      841 aggcgataag cttgacgctg tatgcttgag atttataaat gatgactaca agtttttagt
      901 taatttcaac agagatgaag aggttttcaa atactttcct tctataatgc aaccttcact
      961 caaaacttat agagcgttat caagactagt aaactcaatc aaagatcctt ctagatttaa
     1021 gttcacgaca gagtcattag aaatggagtt actagaatac ttagagttaa gaaatgaatt
     1081 attcaatgat atagaagaag taatgggtag ctatgctcag agggcaccat agatcatgat
     1141 agccctcgat agtacttact tatattttta gagaggttta agtgtaagta gagggagtaa
     1201 cacctggttt ttcagcatcc actccaatga atgctgacac cgatacgcga taattctctt
     1261 tatcgtagca aaatcatctt ccagattgcg gttgagaata ggttcatgat gcgcaatacg
     1321 attgcgcagt tttctcaccg tttccagatc gttgtagaga ttctgccgta actgtgctgc
     1381 attcaatccc tgtgcagccg catgaggaaa agccgtcagt atatgattgt tccagaggcg
     1441 gttatcgaat ctcccggtca gcattttctg ccagaaaaca aacttaagtt caggaataac
     1501 tttccctgtc gtgggttggt ttcgtcgtcc attgatgagg tcatctttgg gtttataacc
     1561 atgaacaggg ataggaagac ttcgttcaaa accaatggac cagggccatc tagctccgta
     1621 taccgtttcg agaacctctg aaacagcatt tcgcgtgata acctcgcata catgcaaggg
     1681 cataaagaaa gcagcggata tctgcgcgtt ccacatgtaa agcctcagag ctttttccgt
     1741 ccggtgaacg cctgccggtc cacctaaaat gctttcatag gttcctattc tggcagcaga
     1801 caagctagcc tttatgctgt ttgacggcat cgtcaacgat ccttataatg aagttactag
     1861 ccacgggact tgttagcagt aatgcttccc cccgaggaca aacgacagag atatacaaca
     1921 aaggcccgct gtatagagta cagcgggcct ttgttgtttt aattcccgca atttaaaata
     1981 ctccgtattt accataacgt ctccttgcgc gcaccgctaa aacgcgctga acgcgattct
     2041 gccgcaaaca ccgcaaaaag atatgttttc cccggggtaa accggaaaaa tgcgtcagga
     2101 tcgctttcag ctcactgcat agctatgcat gaaagtgaat gacgatcggt ttgggcgcat
     2161 acagtgccca tgagacacat tttcggacat tttcagttcg tgaatccctg actcattgct
     2221 acatatctga acagacaccc ctggcagcgc ccccaggggc gtcctgtaaa aaaacacacc
     2281 gcgccgctag cagcacccct aatataagat gatgtttttt atgaaaatag tcagtaccac
     2341 ccctaaaaag cggtgtcggc gcggtgttgt agctgcgcca acaccgcttt tttaaatatc
     2401 acaaagagag caagaagaac taatttgtca taactctcta tttataaaga aaaatcagca
     2461 aaaacttgtt tttgcgtggg gtgtgggcat ttcaggaatg agaacagact cgcgtttttc
     2521 cggagtatca gcggaattat tgattaagaa acactcgacg cagagcgaaa ttttatgcaa
     2581 cagcggctgc actgcacagg gattatttat cagttgatat gctgtgccat gatacgtcct
     2641 aaaaaaacag gacaggacag gacaggacag gacaggatgt gcaattgtaa taccgtcgcc
     2701 cgcaacatct tacaattacc acctgctcag ggtccccccg acacccggaa ctgacggaga
     2761 acaacggaag gtgaacaaac aacaacaaac tgcgctgaat atggcgcgat ttatcaaaag
     2821 ccagagcctg acactgcttg aaaaactgga tgctctggat gccgacgaac aggcggccat
     2881 gtgcgaacga ctgcacgaac tcgcggaaga actccagaac agcatccagg ttcgctttga
     2941 gaccgaaagt gaaacaggaa cataacgaag ctgacggagg cgctcacggc ttgtccgcga
     3001 gcgttttcgg cagcagagaa ccacggcagc ctggcggctg caaattgaaa accgccccgg
     3061 ttcgtcctgt tgttgccgca cgccccgcag aatatgtatg ctctgaggac ctgttttatt
     3121 aaggactcat ccccttatgt tgacccgcaa aagtatcgat acagttctgc tctctgttgg
     3181 tgctgagaag ctctcacaac gagaatggga ctggatgaaa atgctcaaac ccatggaccc
     3241 accacctgcg atggtgacga cctccatcct gaagcgccgt ggcgatacag cagcccttac
     3301 cctgctacag gataccgggg tttaatccat cctcatgttg tccggtttta ctccccgccc
     3361 tctgaaacgt ctgttcacag ccaaccagtg ctggacgtcc ttcctggatg cgggcggtct
     3421 gcgcgatatc gaggttgaag ccgtcaccaa aatgctggcc tgcggcacac ggatactggg
     3481 tgtaaaagag ttcgggtgtg ataacccgga ctgtcagcac gtaaagtacc tgaccaactc
     3541 atgcggcagc cgtgcctgcc cgtcctgcgg aaagaaggcc acagacctgt ggacagcaac
     3601 acagctgaat cgtcttcctg actgcgactg ggtacatctg gtcttcaccc tgccggacac
     3661 gctgtggccg gtgttcgaaa gcaaccgctg gctgctgaat gacgtgtgcc gtctggcggt
     3721 ggagaatctg ctgtatgccg cccgaaaacg ggggctggaa cccggtatct tctgcgccat
     3781 ccacacgtat ggccgtcgtc tcaactggca tccgcatgta catgtgtctg taacctgtgg
     3841 aggtctgaat aagcatggtc agtggaaaaa gctgagcttc ctgaaagacg cgatgcgttc
     3901 acggtggatg tggaatatgc ggcagctgct tctgaaagcg tggtcagagg ggatggcaat
     3961 gccggagtcg ttgtcacata tcacgacgga atcacagtgg agaagcctgg tgctgaaatc
     4021 cggcggaaaa tactggcatg tgtacatgtc gaagaagaca gccggagggc ggaatacggc
     4081 acgctacctg ggtcgttatc tgaagaagcc accaatagcg gcttcccgac tggctcatta
     4141 caacggaggg gcaagcctga gcttccgtta cctggaccac aaaacgggag aaacggcgac
     4201 ggaaacgctg acacagcgtg agctggtcgc gaggctgaaa cagcacatcc cggagaagtt
     4261 ttttaagatg gtgaggtact tcgggttcct tgccaaccgt gtgtgtggag agaagctgcc
     4321 gcaggtgtac cgtgcactgg ggatggataa accggaacca gtggcgaaag tgtgctatgc
     4381 acaaatggtg aaacagttcc tgagtcgtga cccgttcgaa tgcgtgctgt gtggctgccg
     4441 gatggtatac cgccgggcca ttgcggggct gaatgtatca gggctgaaga aaaacgcgcg
     4501 ggatatcagt ctactgaggt atatgccggc ctgacacagg ctaagtgtgc ccgggaatcg
     4561 gtgaaagtat aaaaaacagc ctgttacggt ataaaaatcg accatacaga gagggaaacc
     4621 accggagtgg tgggaaaagc agaaaggctc acgtggcata aggcgtggac aatggtatcg
     4681 actggttttt caatttccta tacgtgaaca ccggacgtgt atttcttcgc aaatggcgtg
     4741 gcatcgatga gttcccggac ttcttccgga ttaccctgaa gcaccgttgc gccttcgcga
     4801 ttacgctccc tccccagcag gtaatcaacc ggaccactgc caccaccttt tcccctggca
     4861 tgaaatttaa caatcatccc gcgccccctg ttccctgacg gccagacgca gccggcgcag
     4921 ctcatccccg atggccatca gtgcggccac cacctgaacc cggtcgccgg aagaccactg
     4981 cccgctgttc accttcctcg ccgtctgatt caggttattc ccgatggcgg ccaactgacg
     5041 cagtaacggc ggagccaaag ttggcagctt cccggaacgg gcaaccggct cacccaggca
     5101 gacccgccgc atccataccg ccagttgttt accctcacag cgttccagta accgggcatg
     5161 ttcatcatca gtaacccgta tcgtgagcat cctctcgcgt ttcatcggta tcattacccc
     5221 catgaacaga aatcccccct tacacggagg catcagtgac taaacaggaa aaaactgccc
     5281 ttaacatggc ccgctttatc agaagccaga cattaacgct gctggagaaa ctcaacgaac
     5341 tggacgcgga tgaacaggca gatatctgtg aatcgcttca cgaccacgcc gatgagcttt
     5401 accgcagctg cctcgcgcgc ttcggggatg acggtgaaaa cctctgacac atacagctcc
     5461 cggagacggt cacagcttgc ctgtgagcgg ataccgggag cagacaagcc cgtcagggcg
     5521 cgtcaggggg tttcggggcg aagccctgaa ccagtcacgt agcgatagcg gagtgtatac
     5581 tggcttaacc atgcggcatc agtgcagatt gtatgaaaag tgccccatgc cgggtgtgaa
     5641 atgccgcaca gatgcgtaag gagaaaatgc gcgtccaggc gcttttccgc ttcctcgctc
     5701 actgactcgc tacgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaagacg
     5761 gtaatgcggt tatccacaga atcaggggat aacgccaaag gaaacatgtg agcaaaaaac
     5821 aagaacccgg aaaaggccac gccgctggcg tttttccata ggctccgccc ccctgacgag
     5881 catcacagaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     5941 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     6001 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     6061 tggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     6121 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     6181 cacgccttaa cgccactggc agcagccact ggtaactgga caaggtggat tgagatatta
     6241 agagttcttg aagtggtggc ctaactgcgg ctacactaga aggacagtat ttggtatctg
     6301 tgctccacca agccagttac ccggttaagc agtccccaac tgacttaacc ttcgactaaa
     6361 ccgcctcccc aggcggtttt ttcgtttaca ggcagcagat tacgcgcaga aaaaaaggat
     6421 ctcaagagga tcctttgatc tttttactga aggagggcaa atctcctaaa actcccagcc
     6481 accttctaac ttcatttttg tatatctaat attgatacac gcatttattg aaccacggca
     6541 gcctggcggc tgcaaattga aaaccgcccc ggttcgtcct gttgttgccg cacgccccgc
     6601 agaatatgta tgctctgagg acctgtttta ttaaggactc atccccttat gttgacccgc
     6661 aaaagtatcg atacagttct gctctctgtt ggtgctgaga agctctcaca acgagaatgg
     6721 gactggatga aaatgctcaa acccatggac ccaccacctg cgatggtgac gacctccatc
     6781 ctgaagcgcc gtggcgatac agcagccctt accctgctac aggataccgg ggtttaatcc
     6841 atcctcatgt tgtccggttt tactccccgc cctctgaaac gtctgttcac agccaaccag
     6901 tgctggacgt ccttcctgga tgcgggcggt ctgcgcgata tcgaggttga agccgtcacc
     6961 aaaatgctgg cctgcggcac acggatactg ggtgtaaaag agttcgggtg tgataacccg
     7021 gactgtcagc acgtaaagta cctgaccaac tcatgcggca gccgtgcctg cccgtcctgc
     7081 ggaaagaagg ccacagacct gtggacagca acacagctga atcgtcttcc tgactgcgac
     7141 tgggtacatc tggtcttcac cctgccggac acgctgtggc cggtgttcga aagcaaccgc
     7201 tggctgctga atgacgtgtg ccgtctggcg gtggagaatc tgctgtatgc cgcccgaaaa
     7261 cgggggctgg aacccggtat cttctgcgcc atccacacgt atggccgtcg tctcaactgg
     7321 catccgcatg tacatgtgtc tgtaacctgt ggaggtctga ataagcatgg tcagtggaaa
     7381 aagctgagct tcctgaaaga cgcgatgcgt tcacggtgga tgtggaatat gcggcagctg
     7441 cttctgaaag cgtggtcaga ggggatggca atgccggagt cgttgtcaca tatcacgacg
     7501 gaatcacagt ggagaagcct ggtgctgaaa tccggcggaa aatactggca tgtgtacatg
     7561 tcgaagaaga cagccggagg gcggaatacg gcacgctacc tgggtcgtta tctgaagaag
     7621 ccaccaatag cggcttcccg actggctcat tacaacggag gggcaagcct gagcttccgt
     7681 tacctggacc acaaaacggg agaaacggcg acggaaacgc tgacacagcg tgagctggtc
     7741 gcgaggctga aacagcacat cccggagaag ttttttaaga tggtgaggta cttcgggttc
     7801 cttgccaacc gtgtgtgtgg agagaagctg ccgcaggtgt accgtgcact ggggatggat
     7861 aaaccggaac cagtggcgaa agtgtgctat gcacaaatgg tgaaacagtt cctgagtcgt
     7921 gacccgttcg aatgcgtgct gtgtggctgc cggatggtat accgccgggc cattgcgggg
     7981 ctgaatgtat cagggctgaa gaaaaacgcg cgggatatca gtctactgag gtatatgccg
     8041 gcctgacaca ggctaagtgt gcccgggaat cggtgaaagt ataaaaaaca gcctgttacg
     8101 gtataaaaat cgaccataca gagagggaaa ccaccggagt ggtgggaaaa gcagaaaggc
     8161 tcacgtggca taaggcgtgg acaatggtat cgactggttt ttcaatttcc tatacgtcaa
     8221 gcccgtcagg gcgcgtcagg gggttttagc gggtgtcagg gcgcagccat gacccagtca
     8281 cgtagcgata gcggagtgta tacgggctta accatgcggc atcagtgcag attgtatgaa
     8341 aagtgcccca tgccaggtgt gaaatgccgc acagatgcgt aaggagaaaa tgcacgtcca
     8401 ggcgcttttc cgcttcctcg ctcactgact cgctacgctc ggtcgttcgg ctgcggcgag
     8461 cggtactgac tcacacaaaa acggtaacac agttatccac agaatcaggg gataaggccg
     8521 gaaagaacat gtgagcaaca aggcaaagct aggacaaaag gccgcgccgc tggcgttttt
     8581 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8641 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8701 tcctgttccg accctgccgc ttaccggata cctctccgcc tttctccctt cgggaagcgt
     8761 ggcgctttct catagctcac gctgttggta tctcagttcg gtgtaggtcg ttcgctccaa
     8821 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaact
//
