LOCUS       CP100018               12915 bp    DNA     circular BCT 21-JUL-2022
DEFINITION  Escherichia coli strain EC602A1 plasmid p602A1-12K, complete
            sequence.
ACCESSION   CP100018
VERSION     CP100018.1
DBLINK      BioProject: PRJNA827955
            BioSample: SAMN27625687
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12915)
  AUTHORS   Tang,B., Chang,J., Yang,H., Wu,J. and Li,J.
  TITLE     A 12-year investigation on the antimicrobial resistance of
            Escherichia coli from diseased swine
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12915)
  AUTHORS   Tang,B., Chang,J., Wu,J., Yang,H., Lin,J., Guan,C., Jiang,H.,
            Chen,H. and Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
REFERENCE   3  (bases 1 to 12915)
  AUTHORS   Tang,B., Chang,J., Yang,H., Wu,J. and Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. JAN-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/29/2022 22:33:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,933
            CDSs (total)                      :: 5,804
            Genes (coding)                    :: 5,484
            CDSs (with protein)               :: 5,484
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 98
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 320
            CDSs (without protein)            :: 320
            Pseudo Genes (ambiguous residues) :: 0 of 320
            Pseudo Genes (frameshifted)       :: 99 of 320
            Pseudo Genes (incomplete)         :: 227 of 320
            Pseudo Genes (internal stop)      :: 56 of 320
            Pseudo Genes (multiple problems)  :: 55 of 320
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..12915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC602A1"
                     /isolation_source="gut"
                     /db_xref="taxon:562"
                     /plasmid="p602A1-12K"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2018-03"
     gene            262..1074
                     /locus_tag="MZH69_29410"
     CDS             262..1074
                     /locus_tag="MZH69_29410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="UTU27320.1"
                     /translation="MHLEEGDLRALERAVELLEQDTITETLTQLVGTPIDYLMSKLPKG
                     AEEQIYSLVEKSLHKAADAALWSLDNEPNRIASTKTNKFFAAVSGAIGGAFGFSALAIE
                     LPVSTTIMLRSIADIARSEGFELDNIDTKLACLEVFSLGGPSDDDDAANTAYYATRSLT
                     AEIVQDLSKEISNIAVKNASSVQTGKWLASLIEKVATRFGIVITEKMAAQVVPVIGALT
                     GATLNTMFTDYYQDMARGHFIVKRLEKKYGNELIKAEYIKISSKLTNS"
                     /name="EcsC family protein"
     gene            1302..1736
                     /locus_tag="MZH69_29415"
     CDS             1302..1736
                     /locus_tag="MZH69_29415"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UTU27321.1"
                     /translation="MGFWDSIKNAAIKAKCGVGIHGGNYKLIDGETCKYSKLCPDCNRT
                     IQKEQHKYGEENYKYDFKCTTVKKCIDCGAEQEGERHERFVEIAVDDYCNVKERCVRCF
                     TERVHGKRHNWYLSGSSDTYRHYKCSVCGEEKEERKTSFR"
                     /name="hypothetical protein"
     gene            1772..2656
                     /locus_tag="MZH69_29420"
     CDS             1772..2656
                     /locus_tag="MZH69_29420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001439342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="UTU27322.1"
                     /translation="MENNTQGIKLIKEAVDVLPKEIRKSVYNKIRNVINYEPVIGIMGK
                     TGAGKSSLCNSIFKGNVCAVSDVEACTRKVQELIISFGDRTIKIIDIPGVGENEAKDIE
                     YEELYKNLLPELDLVLWVVKGDDRAFSADEHFYKNVLKPVGGDEKVLFVLNQVDKIEPF
                     REWDEEKHQPSPSQQINIRKKEEYLIERFGFTKHPVISVSANEGYNISRLVEAIVRSLP
                     KEAQSGVVHQVKDEIKTKEIVETAKDGFADTVDRAIDDIIDTVLPKPIASVAKVAKKAI
                     VSVAKSVWGLIFG"
                     /name="50S ribosome-binding GTPase"
     gene            complement(2991..3245)
                     /locus_tag="MZH69_29425"
     CDS             complement(2991..3245)
                     /locus_tag="MZH69_29425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UTU27323.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3252..3770)
                     /locus_tag="MZH69_29430"
     CDS             complement(3252..3770)
                     /locus_tag="MZH69_29430"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="UTU27324.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(3808..4239)
                     /locus_tag="MZH69_29435"
                     /pseudo=""
     CDS             complement(3808..4239)
                     /locus_tag="MZH69_29435"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4448..4771)
                     /locus_tag="MZH69_29440"
     CDS             complement(4448..4771)
                     /locus_tag="MZH69_29440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UTU27328.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4836..5027
                     /locus_tag="MZH69_29445"
     CDS             4836..5027
                     /locus_tag="MZH69_29445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UTU27325.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENH"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5204..5392
                     /locus_tag="MZH69_29450"
     CDS             5204..5392
                     /locus_tag="MZH69_29450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UTU27326.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVTTSIL
                     KRRGDTAALTLLQDTGV"
                     /name="hypothetical protein"
     gene            5402..6601
                     /locus_tag="MZH69_29455"
     CDS             5402..6601
                     /locus_tag="MZH69_29455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="UTU27327.1"
                     /translation="MLSGFTPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKMLACGT
                     RILGVKEFGCDNPDCQHVKYLTNSCGSRACPSCGKKATDLWTATQLNRLPDCDWVHLVF
                     TLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGLEPGIFCAIHTYGRRLNWHPHVH
                     VSVTCGGLNKHGQWKKLSFLKDAMRSRWMWNMRQLLLKAWSEGMAMPESLSHITTESQW
                     RSLVLKSGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLSFRYLD
                     HKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRALGMDKP
                     EPVAKVCYAQMVKQFLSRDPFECVLCGCRMVYRRAIAGLNVSGLKKNARDISLLRYMPA
                     "
                     /name="IS91 family transposase"
     gene            complement(6766..7560)
                     /locus_tag="MZH69_29460"
                     /pseudo=""
     CDS             complement(6766..7560)
                     /locus_tag="MZH69_29460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(7550..7873)
                     /locus_tag="MZH69_29465"
     CDS             complement(7550..7873)
                     /locus_tag="MZH69_29465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UTU27329.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRICLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQVVREHGVR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            7938..8129
                     /locus_tag="MZH69_29470"
     CDS             7938..8129
                     /locus_tag="MZH69_29470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UTU27314.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGGDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            8789..8944
                     /locus_tag="MZH69_29475"
     CDS             8789..8944
                     /locus_tag="MZH69_29475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="UTU27315.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAFDKSRCAREKF
                     REYLKW"
                     /name="replication initiation protein"
     gene            complement(9752..9985)
                     /locus_tag="MZH69_29480"
     CDS             complement(9752..9985)
                     /locus_tag="MZH69_29480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UTU27316.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNEQVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(9992..10390)
                     /locus_tag="MZH69_29485"
     CDS             complement(9992..10390)
                     /locus_tag="MZH69_29485"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="UTU27317.1"
                     /translation="MTSNETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLA
                     GNTGMMLLGMVIFLFTVSGGTLWYLGGRIQANLEEIRIQEETLQKLNAKTWGVEFVQDG
                     RRKFLVIPQGKSATVIPYQGKDWVQLTE"
                     /name="mobilization protein"
     gene            10530..10718
                     /locus_tag="MZH69_29490"
     CDS             10530..10718
                     /locus_tag="MZH69_29490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UTU27318.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVTTSIL
                     KRRGDTAALTLLQDTGV"
                     /name="hypothetical protein"
     gene            10728..11927
                     /locus_tag="MZH69_29495"
     CDS             10728..11927
                     /locus_tag="MZH69_29495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="UTU27319.1"
                     /translation="MLSGFTPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKMLACGT
                     RILGVKEFGCDNPDCQHVKYLTNSCGSRACPSCGKKATDLWTATQLNRLPDCDWVHLVF
                     TLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGLEPGIFCAIHTYGRRLNWHPHVH
                     VSVTCGGLNKHGQWKKLSFLKDAMRSRWMWNMRQLLLKAWSEGMAMPESLSHITTESQW
                     RSLVLKSGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLSFRYLD
                     HKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRALGMDKP
                     EPVAKVCYAQMVKQFLSRDPFECVLCGCRMVYRRAIAGLNVSGLKKNARDISLLRYMPA
                     "
                     /name="IS91 family transposase"
     Typing          complement(8208..8292)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.837"
                     /coverage="100.0"
                     /evalue="1.41e-35"
     Typing          complement(12123..12201)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.8899999999999995e-34"
     Typing          8316..8861
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.891"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          12215..12772
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.036"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2888..4458)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.809"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6767..7560)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.491"
                     /coverage="100.0"
                     /evalue="1.1900000000000001e-169"
     Typing          complement(9649..10409)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="84.291"
                     /coverage="100.0"
                     /evalue="5.05e-112"
ORIGIN
        1 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgttgg tagcggtggt
       61 ttttttgttt acaggcagca gattacgcgc agaaaaaaag gatcacaaga ggatcctttg
      121 atcttttcat cactcctgct tcacagacaa caatgatgtc ggcatctcgc ctattgaatc
      181 actgaactcg ctcaaggtaa gctcccaaga cgaatcactt acaaaagtag actatcgatt
      241 gattcataaa ggattcatta tatgcatctt gaagaaggag atctgcgggc tttagaaagg
      301 gccgtagaat tattagaaca agatacaatt actgaaacat taacccaatt agttggaacc
      361 ccaatagatt acttaatgag taaacttccc aaaggggcag aagagcaaat ttatagtcta
      421 gtagaaaaat ctctccacaa agctgctgat gcagccttat ggagtctaga caatgaacct
      481 aaccgaatag catcaactaa gacaaataaa ttctttgcgg ccgtatctgg agccattggt
      541 ggcgcttttg gcttttctgc attagcaatt gagttaccgg tttcaacgac aattatgctt
      601 cgttcaattg ctgacatagc aagaagtgaa ggttttgagc tagataatat tgataccaaa
      661 ctggcctgct tagaggtgtt ttccttggga ggaccatctg atgacgatga tgcagctaat
      721 actgcatact atgcaacaag aagtttgact gccgagatcg tacaagattt atccaaagaa
      781 atttctaaca ttgctgtcaa aaacgcatct tcagtacaaa caggaaaatg gttagcttcg
      841 ttaatagaaa aagttgcaac aagatttggt atagttatta cggaaaaaat ggcagctcaa
      901 gtcgttcccg ttattggagc actaactgga gccacattaa acacaatgtt taccgactat
      961 tatcaagata tggctcgagg tcatttcatt gttaaaaggc tcgagaaaaa atatggcaat
     1021 gagttaataa aggctgaata tataaaaatc tcaagcaaac tcacaaattc gtaaaatatt
     1081 tatcttggat gaattagaca atgactttcc aatactatct ttgcgggaaa aacacgaagt
     1141 ttatccagtg cccccacttt ttcagctcta tatttaatat aacaacaaca ttatacagag
     1201 cataaatcca tactcatttt tatatatcca cacactatac gtgtataatc atgtcgatga
     1261 attgttcaac ttatatcaac taatcgtagg gagatttttc catgggcttt tgggatagca
     1321 taaaaaatgc agcaatcaag gctaagtgtg gggtaggtat acacggagga aactataagc
     1381 ttattgatgg tgagacttgt aaatattcta aactatgccc tgactgcaat cgcactatac
     1441 aaaaagaaca gcataaatac ggagaggaaa attataaata cgatttcaag tgcacaactg
     1501 taaaaaagtg tatcgactgt ggcgctgaac aagaaggcga aaggcatgaa agatttgttg
     1561 aaattgcagt cgatgattat tgcaatgtga aagaaagatg cgtgagatgt tttacagagc
     1621 gagttcatgg taaaagacac aattggtatc ttagtgggag ttctgatacc tatcgccatt
     1681 acaaatgttc tgtatgcggg gaagaaaaag aagaaagaaa aacaagcttc cgttaattca
     1741 tatgaacaac aaaatgagga aaatagtaac tatggaaaac aatacgcaag gtataaaatt
     1801 aatcaaagaa gctgtggatg ttctacctaa agagattcgc aagtctgtct ataacaaaat
     1861 tagaaatgtt attaattacg aaccagtaat agggattatg ggtaaaaccg gcgctggtaa
     1921 atccagccta tgtaattcta tctttaaagg taatgtatgt gcagtgagtg atgtagaagc
     1981 atgcacgaga aaggttcagg aattaataat ttcatttggt gatagaacaa ttaaaataat
     2041 agatatacca ggtgtaggtg aaaatgaagc taaagatata gaatatgaag agttgtataa
     2101 aaatcttctc ccagaattag atctggtgct ttgggttgtt aaaggtgatg atcgagcatt
     2161 ttcagctgat gagcactttt ataaaaatgt tttaaaacca gttggtggtg atgaaaaagt
     2221 gttgtttgtt ttaaaccaag tcgacaagat tgagccattc cgcgaatggg atgaagaaaa
     2281 acaccaacca tcaccctcac aacaaataaa tattagaaaa aaggaagaat acttaataga
     2341 acgattcggt tttaccaaac atcccgttat atctgtatct gcgaatgaag gttataacat
     2401 ttcaaggtta gtggaggcaa tagtaagatc gctcccaaag gaagcacaga gcggagtggt
     2461 gcatcaagta aaagacgaaa taaaaacaaa agaaattgta gaaacggcaa aggacgggtt
     2521 cgcagacacc gtagatagag ctattgatga tataatagac accgtactac ctaaaccaat
     2581 agcgtcagtg gctaaagtcg ccaagaaagc cattgtttct gtagctaaaa gtgtttgggg
     2641 attgatcttc ggataatcaa tcaggaaagc ccatcagtat ttaacataac aacagtttgt
     2701 acgtaccgct aaaacgcgtt cagcgcgatc acggcagcag acaggtaaaa atagcaacaa
     2761 accacccgaa aaactgccgc gatcgcgcct gataaatttt aaccgtatga atacctatgc
     2821 aaccagatgg cacagagcac attagtccgc cttaatccaa tgaacctgcc atttttcatg
     2881 gtttcaccat cccagcgaag ggccatccag cgtgcgttcc tgtatttccg gctgacgctc
     2941 ccgttccagg gataacacat gttcgcgctc ctgtatcagc cgttcctctc ttatctccag
     3001 ttctcgctgt ataactggct caagcgttct gtctgctcgc tcaagcgttc tgtctgctcg
     3061 ctcaagtgct gcacctgctg actcaactgc atgacccgct cgttcagcat cgcgttgtcc
     3121 cgttgcgtaa gcgaaaacat cttctgcaat tccacgaagg cgctctccca ttcgctcagc
     3181 cgctgcatat agtcctgttg cagctgctct aaggcgttca gcaaatgtgt ttccagctct
     3241 gtcactctgt gtcactcctt cagatgtacc cactctttcc cctgaaaggg aatcacctcc
     3301 gctgatttcc cgtacggaag gacaaggaat ttcctgttcc cgtcctgcac aaactccacg
     3361 ccccatgtct tcgcgttcag tttctgcaat gtctcttcct gcttcctgat ttcttccagg
     3421 ttcgcctgta tcctccctcc aagataccag agcgtcccgc cactcgcggt aaacaggaga
     3481 aagactatcc ccagtaacat catgcccgta ttccctgcca gctttaacac gtccctcctg
     3541 tgctgcatca tcgcctcttt caccccttcc cggtgttttt ccagcgattc ctctgtcgag
     3601 gctgtgaaca gggctatagc gtctctgatt ttcgtctcgt ttgatgtcac agcctcgctt
     3661 acagattcgc cgagcctcct gaactcgttg ttcagcattt tctctgtaga ttcggctctc
     3721 tctttcagct ttttctcgaa ctccgcgccc gtctgcaaaa gattgctcat aaaatgctcc
     3781 tttcagcctg atattcttcc cgccgttcgg atctgcaatg ctgatactgc ttcgcgtcac
     3841 cctgaccact tccagccccg cctcagtgag cgcctgaatc acatcctgac ggccttttat
     3901 ctctccggca tggtaaagtg catctatacc tcgcgtgacg ccctcagcaa gcgcctgttt
     3961 cgtttcaggc aggttatcag ggagtgtcag cgtcctgcgg ttctccgggg cgttcgggtc
     4021 atgcagcccg taatggtgat ttaccagcgt ctgccaggca tcaattctag gcctgtctgc
     4081 gcggtcgtag tacggctgga ggcgttttcc ggtctgtagc tccatgttcg gaatgacaaa
     4141 attcagctca agccgtccct tgtcctggtg ctccacccac aggatgctgt actgattttt
     4201 ttcgagaccg ggcatcagta cacgctcaaa gctcgccatc actttttcac gtcctcccgg
     4261 cggcagctcc ttctccgcga acgacagaac accggacgtg tatttcttcg caaatggcgt
     4321 ggcatcgatg agttcccgga cttcttccgg attaccctga agcaccgttg cgccttcgcg
     4381 gttacgctcc ctccccagca ggtaatcaac cggaccactg ccaccacctt ttcccctggc
     4441 atgaaattta actatcatcc cgcgccccct gttccctgac agccagacgc agccggcgca
     4501 gctcatcccc gatggccatc agtgcggcca ccacctgaac ccggtcaccg gaagaccact
     4561 gcccgctgtt caccttacgg gctgtctgat tcaggttatt tccgatggcg gccagctgac
     4621 gcagtaacgg cggtgccagt gtcggcagtt ttccggaacg ggcaaccggc tcccccaggc
     4681 agacccgccg catccatacc gccagttgtt taccctcaca gcgttccagt aaccgggcat
     4741 gttcatcatc agtaacccgt atcgtgagca tcctctcgcg tttcatcggt atcattaccc
     4801 ccatgaacag aaatccccct tacacggagg catcagtgac aaaacaggaa aaaaccgccc
     4861 ttaacatggc ccgctttatc agaagccaga cattaacgct gctggagaag ctcaacgaac
     4921 tggacgcgga tgaacaggca gatatctgtg aatcgcttca cgaccacgcc gatgagcttt
     4981 accgcagctg cctcgcacgc ttcggggatg acggtgaaaa ccactgacac atgcagctcc
     5041 cggagacggt cacagcttgt ctgtgagcgg atgccgggag cagagaacca cggcagcctg
     5101 gcggctgcaa attgaaaacc gccccggttc gtcctgttgt tgccgcacgc cccgcagaat
     5161 atgtatgctc tgaggacctg ttttattaag gactcatccc cttatgttga cccgcaaaag
     5221 tatcgataca gttctgctct ctgttggtgc tgagaagctc tcacaacgag aatgggactg
     5281 gatgaaaatg ctcaaaccca tggacccacc acctgcgatg gtgacgacct ccatcctgaa
     5341 gcgccgtggc gatacagcag cccttaccct gctacaggat accggggttt aatccatcct
     5401 catgttgtcc ggttttactc cccgccctct gaaacgtctg ttcacagcca accagtgctg
     5461 gacgtccttc ctggatgcgg gcggtctgcg cgatatcgag gttgaagccg tcaccaaaat
     5521 gctggcctgc ggcacacgga tactgggtgt aaaagagttc gggtgtgata acccggactg
     5581 tcagcacgta aagtacctga ccaactcatg cggcagccgt gcctgcccgt cctgcggaaa
     5641 gaaggccaca gacctgtgga cagcaacaca gctgaatcgt cttcctgact gcgactgggt
     5701 acatctggtc ttcaccctgc cggacacgct gtggccggtg ttcgaaagca accgctggct
     5761 gctgaatgac gtgtgccgtc tggcggtgga gaatctgctg tatgccgccc gaaaacgggg
     5821 gctggaaccc ggtatcttct gcgccatcca cacgtatggc cgtcgtctca actggcatcc
     5881 gcatgtacat gtgtctgtaa cctgtggagg tctgaataag catggtcagt ggaaaaagct
     5941 gagcttcctg aaagacgcga tgcgttcacg gtggatgtgg aatatgcggc agctgcttct
     6001 gaaagcgtgg tcagagggga tggcaatgcc ggagtcgttg tcacatatca cgacggaatc
     6061 acagtggaga agcctggtgc tgaaatccgg cggaaaatac tggcatgtgt acatgtcgaa
     6121 gaagacagcc ggagggcgga atacggcacg ctacctgggt cgttatctga agaagccacc
     6181 aatagcggct tcccgactgg ctcattacaa cggaggggca agcctgagct tccgttacct
     6241 ggaccacaaa acgggagaaa cggcgacgga aacgctgaca cagcgtgagc tggtcgcgag
     6301 gctgaaacag cacatcccgg agaagttttt taagatggtg aggtacttcg ggttccttgc
     6361 caaccgtgtg tgtggagaga agctgccgca ggtgtaccgt gcactgggga tggataaacc
     6421 ggaaccagtg gcgaaagtgt gctatgcaca aatggtgaaa cagttcctga gtcgtgaccc
     6481 gttcgaatgc gtgctgtgtg gctgccggat ggtataccgc cgggccattg cggggctgaa
     6541 tgtatcaggg ctgaagaaaa acgcgcggga tatcagtcta ctgaggtata tgccggcctg
     6601 acacaggcta agtgtgcccg ggaatcggtg aaagtataaa aaacagcctg ttacggtata
     6661 aaaatcgacc atacagagag ggaaaccacc ggagtggtgg gaaaagcaga aaggctcacg
     6721 tggcataagg cgtggacaat ggtatcgact ggtttttcaa tttcctatac gtcgagcttg
     6781 ctgaactcgt cgttcagcat tgtctctgta gattcggctc tctctttcag ctttttctcg
     6841 aactccgcgc ccgtctgtaa aagattgctc atagaatgct cctttcagcc tgatattctt
     6901 cccaccgttc gggtctgcaa tgctgatact gcttcgcgtc accctgacca cttcaagccc
     6961 tgcctctgtg agcgcctgaa tcacatcctg acggcctttt atctccccga catgataaag
     7021 agcgtctatc ccgcgtgtga cgctctctgc aagcgcctgt ttcgtttccg gcaggttatc
     7081 agggagagtc agtatccggc ggttctccgg ggcgttgggg tcatgcagcc cgtaatggtg
     7141 attcaccagc gtctgccagg catcaattct cggcctgtct gcccggtcgt agtacggctg
     7201 gaggcgtttt ccggtctgta gctccatgtt cggaatgaca aaattcagct caagccgtcc
     7261 cttgtcctgg tgctccaccc acaggatgct gtactgattc ttttcgagac cgggcatcag
     7321 tacacgctca aagctcgcca tcaccttttc acgtcctccc ggcggcagct ccttctccgc
     7381 gaacgacaga acacctgacg tgtatttctt cgcaaatggc gtggcatcga tgagttcccg
     7441 gacttcttcc ggattaccct gaagcaccgt tgccccttcc cggtttcgct cccttcccag
     7501 caggtaatca accggaccac tgccaccgcc ttttcccctg gcatgaaact taactatcat
     7561 cccgcactcc atgttccctg acgacctgcc gtaagctgcg caactctctc tcgatggcca
     7621 tcagtgcggc caccacctga acccggtcac cggaagacca ctgcccgctg ttcaccttcc
     7681 tcgccgtctg attcaggtta ttcccgatgg cagccagctg gcgcagtaac ggcggtgcca
     7741 gtgtcggcag cttcccggaa cgggcaaccg gctcacccag gcagatccgc cgcatccata
     7801 ccgccagctg tttaccctca cagcgttcca gtaaccgtgc atgttcatca tcagtaaccc
     7861 gtatcgtgag catcctctcg cgtttcatcg gtatcattac cccatgaaca gaaatccccc
     7921 cttacacgga ggcatcagtg actaaacagg aaaaaaccgc ccttaacatg gcccgcttta
     7981 taagaagcca gacattaacg ctgctggaga agctcaacga actggatgca gatgaacagg
     8041 ccgatatttg tgaatcactt cacgaccacg ccgacgagct ttaccgcagc tgcctcgcac
     8101 gcttcggggg tgacggtgaa aacctctgac acatacagct cccggagacg gtcacagctt
     8161 gtctgtgagc ggatgccggg agcagacaag cccgtcaggg cgcgtcaggg ggtttcgggg
     8221 cgaagccctg aaccagtcac gtagcgatag cggagtgtat actggcttaa ccatgcggca
     8281 tcagtgcaga ttgtatgaaa agtgcgccat gccgggtgtg aaatgccgca cagatgcgta
     8341 aggagaaaat gcagcttccg acgctcttcc gcttcctcgc tcactgactc gctacgctcg
     8401 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     8461 gaatcagggg ataacgccgg aaagaacatg tgcgcaaaag gcaaaggccg ggagaaaggc
     8521 tacgccgatg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     8581 ctctagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     8641 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     8701 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgttggtatc tcagttcggt
     8761 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8821 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact taacgccact
     8881 ggcagcagcc tttgataaaa gtcggtgtgc gagagagaaa tttagagagt acttgaagtg
     8941 gtgaccggag tccggctaca ctgaaaggac agttttggtg actcgtctcg cacaagacag
     9001 ttacctggtt aaacagtctg caagctggct taaccttcga tcaaaccgcc ctgcaaggcg
     9061 gttttttcgt ttacaaggca agagattacg cgcagcagcc atcgatctca agaggatctt
     9121 acttggatcc ctagaaaaca gcccaccatc acatcacgta acatacaaaa tcagccaccc
     9181 ggattgaata tacataatag tactatgtgc gttattgtac gcaccactga aacgcgttcg
     9241 gtgcgatcac ggcagcagac aggtaaaaat ggcaacaaac cacccgaaaa actgccgcga
     9301 tcgcacccgg taaattttaa ccacatgcat agctatgcag ccatgtgaat cacgcaggaa
     9361 tttccagcca gagactgctg aaacggattt ttttcatgct ttcggaagcg aagaagacgg
     9421 gaaccggagc gggaaaacag aagttcaggg gaatgagcgc gatctggcaa tagaggcaaa
     9481 acacagcaac aaaagataca ccagaatcgc gcccgtatgc gttttaacgc gttcctgtgt
     9541 tttcagcaag gtctgactga agcgcgttcc cggtgccccc gaaccaacca ttatcccaaa
     9601 aaatgcaccg gaaacgcgct gacaacattt tagcctgtct ctgattacca tccaagcgaa
     9661 gggccatcct gtgtccattc ctgtatttcc ggctggcgct cacgctccag ggataacgca
     9721 tgttcgcgct cctgtatcag ccgttcgtcc cttatctcct gttctcgctg tataactggc
     9781 tcaagcgttc tgtccgctcg ctcaagtgct gcacctgctg actcaactgc atgacctgct
     9841 cgttcagcat cgcgttgtcc cgttgcgtaa gcgaaaacat tttctgcaat tccacgaagg
     9901 cgctctccca ttcgttcagc cgctgcatat agtcctgttg cagttgctct aaggcgttca
     9961 gcaaatgtct ttccagctcc gtcactctgt gtcactccgt cagttgtacc cagtccttcc
    10021 cctgataggg aatcaccgtt gccgatttcc cctgcgggat gaccagaaat ttccttctcc
    10081 cgtcctgcac aaactccacg ccccatgtct tcgcgttcag tttctgcaat gtttcttcct
    10141 gtatcctgat ttcttccagg ttcgcctgta tccgcccgcc aagataccag agcgtcccgc
    10201 cactcacggt aaacaggaaa atgaccatcc ccagtaacat catgcccgta ttccctgcca
    10261 gctttaacac gtccttcctg tgctgcatca tcgcctcttt caccccttcc cggtgttttt
    10321 tcagtgattc ctctgtcgaa gctgtgaaca gggctatagc gtctttgatt ttcgtctcgt
    10381 ttgatgtcac agccttgctt acagattttt gaaccacggc agcctggcgg ctgcaaattg
    10441 aaaaccgccc cggttcgtcc tgttgttgcc gcacgccccg cagaatatgt atgctctgag
    10501 gacctgtttt attaaggact catcccctta tgttgacccg caaaagtatc gatacagttc
    10561 tgctctctgt tggtgctgag aagctctcac aacgagaatg ggactggatg aaaatgctca
    10621 aacccatgga cccaccacct gcgatggtga cgacctccat cctgaagcgc cgtggcgata
    10681 cagcagccct taccctgcta caggataccg gggtttaatc catcctcatg ttgtccggtt
    10741 ttactccccg ccctctgaaa cgtctgttca cagccaacca gtgctggacg tccttcctgg
    10801 atgcgggcgg tctgcgcgat atcgaggttg aagccgtcac caaaatgctg gcctgcggca
    10861 cacggatact gggtgtaaaa gagttcgggt gtgataaccc ggactgtcag cacgtaaagt
    10921 acctgaccaa ctcatgcggc agccgtgcct gcccgtcctg cggaaagaag gccacagacc
    10981 tgtggacagc aacacagctg aatcgtcttc ctgactgcga ctgggtacat ctggtcttca
    11041 ccctgccgga cacgctgtgg ccggtgttcg aaagcaaccg ctggctgctg aatgacgtgt
    11101 gccgtctggc ggtggagaat ctgctgtatg ccgcccgaaa acgggggctg gaacccggta
    11161 tcttctgcgc catccacacg tatggccgtc gtctcaactg gcatccgcat gtacatgtgt
    11221 ctgtaacctg tggaggtctg aataagcatg gtcagtggaa aaagctgagc ttcctgaaag
    11281 acgcgatgcg ttcacggtgg atgtggaata tgcggcagct gcttctgaaa gcgtggtcag
    11341 aggggatggc aatgccggag tcgttgtcac atatcacgac ggaatcacag tggagaagcc
    11401 tggtgctgaa atccggcgga aaatactggc atgtgtacat gtcgaagaag acagccggag
    11461 ggcggaatac ggcacgctac ctgggtcgtt atctgaagaa gccaccaata gcggcttccc
    11521 gactggctca ttacaacgga ggggcaagcc tgagcttccg ttacctggac cacaaaacgg
    11581 gagaaacggc gacggaaacg ctgacacagc gtgagctggt cgcgaggctg aaacagcaca
    11641 tcccggagaa gttttttaag atggtgaggt acttcgggtt ccttgccaac cgtgtgtgtg
    11701 gagagaagct gccgcaggtg taccgtgcac tggggatgga taaaccggaa ccagtggcga
    11761 aagtgtgcta tgcacaaatg gtgaaacagt tcctgagtcg tgacccgttc gaatgcgtgc
    11821 tgtgtggctg ccggatggta taccgccggg ccattgcggg gctgaatgta tcagggctga
    11881 agaaaaacgc gcgggatatc agtctactga ggtatatgcc ggcctgacac aggctaagtg
    11941 tgcccgggaa tcggtgaaag tataaaaaac agcctgttac ggtataaaaa tcgaccatac
    12001 agagagggaa accaccggag tggtgggaaa agcagaaagg ctcacgtggc ataaggcgtg
    12061 gacaatggta tcgactggtt tttcaatttc ctatacgtca agcccgtcag ggcgcgtcag
    12121 ggggtttcgg ggcgaagccc tgaaccagtc acgtagcgct agcggagtgt atactggctt
    12181 aactatgcga catcagtgcg gattgtatga aaagtgcgcc atggacggtg tgaaatgccg
    12241 cacagatgcg taaggagaaa atgcaaatgc ctacgctctt ccgcttcctc gctcactgac
    12301 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaga gacggtaatg
    12361 cggttaccca cagaatcagg ggataaggcc ggaaagaaca tgtgagcaaa aaggcaaaac
    12421 caggaaaaag gccgcgcccc tggcgttttt ccataggctc cgcccccctg acgagcatca
    12481 caaaaattga cgctcaagtc agaggtggcg aaacccgaca ggacttaaag ataccaggcg
    12541 tttccccctg gtggctccct cgtgcgctct cctgttccga ccctgccgat taccggatac
    12601 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgttggtat
    12661 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    12721 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    12781 aaagcgccac tggcagcagc cactggtaac tggacaaggt ggattgagat attaagagtt
    12841 cttgaagtgg tggcctaact gcggctacac tagaaggaca gtatttggta tctgtgctcc
    12901 accaagccag ttacc
//
