LOCUS       CP100012                1565 bp    DNA     circular BCT 21-JUL-2022
DEFINITION  Escherichia coli strain EC737A1 plasmid p737A1-1K, complete
            sequence.
ACCESSION   CP100012
VERSION     CP100012.1
DBLINK      BioProject: PRJNA827955
            BioSample: SAMN27625662
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Ni,J., Lin,J., Pan,D., Yang,H. and TANG,B.
  TITLE     cfr-harboring E. coli strains isolated from food animals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Tang,B., Chang,J., Wu,J., Yang,H., Lin,J., Guan,C., Jiang,H.,
            Chen,H. and Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
REFERENCE   3  (bases 1 to 1565)
  AUTHORS   Ni,J., Lin,J., Pan,D., Yang,H. and TANG,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. JAN-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/29/2022 21:57:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,772
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,434
            CDSs (with protein)               :: 4,434
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 73 of 219
            Pseudo Genes (incomplete)         :: 138 of 219
            Pseudo Genes (internal stop)      :: 50 of 219
            Pseudo Genes (multiple problems)  :: 37 of 219
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC737A1"
                     /isolation_source="gut"
                     /db_xref="taxon:562"
                     /plasmid="p737A1-1K"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2020-10"
     gene            complement(687..1166)
                     /locus_tag="MZH82_23860"
     CDS             complement(687..1166)
                     /locus_tag="MZH82_23860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="UTU21876.1"
                     /translation="MYIGTEEQRKKRLKELEKLNKESESDPKNNGPFTQVYQKGWERIR
                     ELSKDKQGVVAIPLYSFLAEHIDPSCGAVVADQQFLADKLGVSRSTIKRWLNYLESKNA
                     LVRIPVAGKVCAYALDPHEVWKGYNTSKNYAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     Typing          177..437
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.275"
                     /coverage="100.0"
                     /evalue="1.83e-109"
ORIGIN
        1 atgtgcaaat gtaataccgt cacccgcgac gatattccgt ttgccatctg gtcagggctt
       61 cgccccgaca ccccgcatca gtgcgtacgt gctcaatgtg gtttccggtt ctcttccgct
      121 tcctcgccca ctcgctcact aacgttcggt cgctggggct gcggcgagcg gtgccacctc
      181 tcgcatgttg tcggtgattc cgctgttgtt ctttcgggga taagtcgtca gatagccctt
      241 caaacgcgct cagaagctct cagacgcacg aaacggggtt taaatgcccg tcagggcatg
      301 gaaggcgcga aaaacgctta cagagcgttt caggaggtca ggcgcgccga tacccctagc
      361 caggggcttt caggtcgcca ggaaccgcgc cagccccctg caaagcccat ttgcacggta
      421 caggcagcca ctcggttggt aggtggtcgt agaccgtcgg tgttacagcg atgggggtct
      481 ggggttctcc ccagacggtt tccggttgcg ctcaaggcgc tgccggaacc gtggcggctc
      541 gatcgttctg gagatcgggg agcaaaaaca cgcaagggat tttgaggcgg caaaagagca
      601 atgcaggata gacaacccga tgtcaaatgt taacatttga atcagattgt ccaaaaacgg
      661 ggtttttgcc tgtgcctgcc gctggctcaa tttgaaaaca tggccatgat tcggcgctga
      721 atatcaccgt ctttgttgac cagtgtttta gtgacaaacg ccgcgtagtt tttagatgtg
      781 ttgtatccct tccagacttc atgtggatcg agtgcatacg cacaaacctt accagcaaca
      841 ggaattctga ctaacgcgtt ttttgattct aagtagttga gccagcgctt aattgtgctt
      901 cggctaacac ccaatttatc tgctaaaaac tgctgatcgg caacaacagc accgcatgag
      961 ggatctatgt gctcagctaa aaacgagtaa agcggtattg ctacaactcc ctgcttgtcc
     1021 ttcgacagtt ctcggatcct ttcccagccc ttctgataaa cctgggtgaa tgggccgtta
     1081 tttttcggat ctgattccga ttctttgttt aatttttcca gttcttttag acgctttttt
     1141 cgctgctctt cagtcccgat atacatggta aatctccacg gtctaatgat ggctcaaata
     1201 taagccatca tagctcaaac gtaaaccatg ataggtcgac ctagctcaca catgcgtgag
     1261 ctaaccttag accacacatg cgtgagctaa cccataatgc aagtagctaa aaaatcaata
     1321 acttacaaaa tcacccttaa atatcttttc tgctgaaagt gttgcgcaaa gcggaacact
     1381 tttttggggt gtcggggcga agccctgacc cagtcacgta gcgatagcgg agtgtatact
     1441 ggcttactcc tctgcaagcc ttaccagctg tgatttttgc cagatttcac gctctcaacg
     1501 ttcaacagct ccatcatggt tttgccgtcc gttcaccgct aaaacgcctc agaaatcggg
     1561 acagg
//
