LOCUS       CP100011                3630 bp    DNA     circular BCT 21-JUL-2022
DEFINITION  Escherichia coli strain EC737A1 plasmid p737A1-3K, complete
            sequence.
ACCESSION   CP100011
VERSION     CP100011.1
DBLINK      BioProject: PRJNA827955
            BioSample: SAMN27625662
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Ni,J., Lin,J., Pan,D., Yang,H. and TANG,B.
  TITLE     cfr-harboring E. coli strains isolated from food animals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Tang,B., Chang,J., Wu,J., Yang,H., Lin,J., Guan,C., Jiang,H.,
            Chen,H. and Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
REFERENCE   3  (bases 1 to 3630)
  AUTHORS   Ni,J., Lin,J., Pan,D., Yang,H. and TANG,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. JAN-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/29/2022 21:57:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,772
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,434
            CDSs (with protein)               :: 4,434
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 73 of 219
            Pseudo Genes (incomplete)         :: 138 of 219
            Pseudo Genes (internal stop)      :: 50 of 219
            Pseudo Genes (multiple problems)  :: 37 of 219
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC737A1"
                     /isolation_source="gut"
                     /db_xref="taxon:562"
                     /plasmid="p737A1-3K"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2020-10"
     gene            723..1244
                     /locus_tag="MZH82_23835"
     CDS             723..1244
                     /locus_tag="MZH82_23835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UTU21873.1"
                     /translation="MSDSQNKLVFSESPQVELGSNFFINVPVILQYKDTPLISVESAVD
                     ASFTTSIPIYHNDGTYLAKAKGTQLYLTEEGRKAGVTLRHEDKITVCLINGKEVFELRR
                     QSAAALKAEAELYVPDGKLISYRGSSPKLFSTENNQLAIGGLVMSGSTFSGCKIGILLD
                     ENSIAIGVNR"
                     /name="hypothetical protein"
     gene            complement(1807..2019)
                     /locus_tag="MZH82_23840"
     CDS             complement(1807..2019)
                     /locus_tag="MZH82_23840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UTU21874.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2023..2508)
                     /locus_tag="MZH82_23845"
     CDS             complement(2023..2508)
                     /locus_tag="MZH82_23845"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="UTU21872.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEKSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            complement(2546..2977)
                     /locus_tag="MZH82_23850"
                     /pseudo=""
     CDS             complement(2546..2977)
                     /locus_tag="MZH82_23850"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3196..3507)
                     /locus_tag="MZH82_23855"
     CDS             complement(3196..3507)
                     /locus_tag="MZH82_23855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UTU21875.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARAGRLPSI
                     SPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAVLEKGGG"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(1692..3196)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.223"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3206..3507)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.137"
                     /coverage="95.0"
                     /evalue="1.56e-56"
ORIGIN
        1 cgcagccctg aaccagtcac gtagcgctag cggagtgtat actggcttaa aatgtaacat
       61 cgcagaaaca tcagacatag ctgccacata aagcaggtga aaaatgaggt tcgtatgcgt
      121 cagcagaaga tgcactgccg gagaaatgcc gcttcctcgc tcactgactc gctacgctcg
      181 gtcgttcggc tgcggcgagc ggtgaaggcg tctttgtggg gatttttttg attctctggc
      241 tttaaatgta caaaagcccc gtgacagaat cacggggctt cggtactcta attcttcgca
      301 gatgcctgcc aggaatagac gtcagaaatc aaatcctagc agggctggct agcattgtca
      361 ctaccagctt aataccattc aacgacatta aaacaatatc tgcaccttag acactcaggt
      421 ctacgccccg gactccggca ggataaacag gcaattggtg gatatgtaac ttaaccgcaa
      481 aagagttaag ctctaccccg tctcgctgta cgataacaag cacctgataa aacgagtgtc
      541 taagaaagta gtaagcggag tagtgtgatt gaaaacgtct gacactactc ccacacaatc
      601 gaaccttttg cagggtgaga cacacaagaa acgagctgga cgaaggtcgc tacgctcccc
      661 gtgattttta tatacccacc acgggggcat taggtttttt ttgacaaaaa aaggatgtat
      721 ccatgagtga ttcgcagaat aaactcgtat ttagtgaaag ccctcaagtt gagcttggtt
      781 ctaatttttt catcaacgtc ccagtaatac ttcagtataa agacacccca cttattagtg
      841 ttgagagtgc ggttgatgct agtttcacta ccagtatccc tatctatcac aacgatggaa
      901 catacctggc caaggctaaa ggaacgcagc tctaccttac tgaagaaggt agaaaggcag
      961 gagtgactct ccggcatgaa gataagataa ctgtttgtct tattaacggg aaagaggtat
     1021 tcgagctcag aagacaaagc gcagcagcac taaaagcaga ggctgagctt tatgttcctg
     1081 atggaaagct aataagttat cgaggatcct cgccaaagtt gttcagcacg gaaaacaatc
     1141 aactagctat aggcggttta gtgatgtccg gatcaacatt cagcgggtgt aaaataggga
     1201 tattgctaga tgaaaacagc atagcgattg gtgtaaatcg gtagccgcgt tggttatttc
     1261 ttgcgcgcgc aagaaaagat gatggtttat cccgctcacc tgtatttacc ataacatccg
     1321 ttgcccgcac cgctccggac aggttcgtca caaacggggc ggcagtcagg taaaaccggc
     1381 aacaaaccac ccgatacacc gccccgtccg tgacagataa attttacccg gctgcatggt
     1441 catgcagcca gatgcatacg gtctgatttc aggccagaat ccggatgaac gccttaaaaa
     1501 caggaaaatg cttctccagc cagtcccaga tctccagcgc catgtacagg cagttcagca
     1561 ccgtctcaat cacgttaaac agtctcataa tccctccgtt gtatacgccg tgatgttttc
     1621 atcacgaccg tatttagcgg gaaatttatg agcccttctt cgtggggttt gtcactggtg
     1681 aagcctgtca gcccagcgtc ggaccgttat agctccgctg acgggaacgg gcttccgcca
     1741 gctctttttc gtgctgtcgc tgctcgtatg cccgttcggc ggccaccgcc atttcatgcc
     1801 gctgcactac tggctcaaac gctgtaattg cctgctcaag ttcgccacct gctgactcaa
     1861 gcgcgtcacg cgctcgttca gcgccgcgtt ctcccgttgc ataagaccag acatcttctg
     1921 ccattccgcg aaggcgctct cccactcgtc cagcctttgc gagtagtcct gatgtagctg
     1981 ctctaatgcg gtcagcaact gtttttccag ttccgtcatg agctatttcc tctccagtac
     2041 catccagttc ccttcctccc cgtaagctcc cgctttcggg ttcactttga cgcaggacag
     2101 tttcttctcc ccgcagagcg acagctgcac gccgtggttc ttctccgaca gcatggcctg
     2161 cgtccgctcc tgcgcccgga tagtctggta atttccggtg atctgctgcc cctgccacca
     2221 cagaatgctc ccgctcgtgg cgatcagcag taccgagacc agagtgatgg tcagccacgt
     2281 ccggccgacc atacgcagca cgctcaggcg gttcgactgc atcgccgcgg tcataccctg
     2341 ctcgtgggcg ctgatggcgt cgctgattct cttcgcgctt gcgctcagtt ccgcttttac
     2401 agacttctcg tgctcgctga atgcggcttt cagcatctcg ccggtactct gctgctgcgc
     2461 tttcgattgc tgctccaagt ccttcgccag cgctaaaaga ctgctcataa atggctccct
     2521 tcagtcggat gtttcgcccc ccgtccgggt cggcaatgct gatactgttt tttgtggtgc
     2581 ggaccacctc aaaacctgcc gctgtgagcg cccctgtgac gtcctcgcgc gttttgagct
     2641 ccccggatga ggctaaggcc agcaaccctc gtgtaatcgc ctgagcggct tccagcttcg
     2701 ttttcggcag tgcggacggc gtgaccagta gccgccggtt ctccggggcg tttggatcat
     2761 gcagccccag tctgccgttc accacggtct gccaggcatc gatgcgcggc cgatcggctc
     2821 gatcgtaata cggctgcagg cgtttcccgg tcagcagttc agtgttgggg atcaggaaat
     2881 tcagttccag ccgccccttg tcggtgtgct ccacccacag aatgctgtac tggtctttat
     2941 cgagcccggg catcagaacc cgctcaaagc tggccatgat ctgctcccgc tgcccgggcg
     3001 gcagttcagc ctccgcgaac gacagtacgc cggaggtgta ctttttcgca tacggcgagg
     3061 catcgataag ctcccggact tcctccggtt ttccctgcag cacggtcgcc ccctcgcgct
     3121 gccggtcttt tcccagcagg taatccaccg gaccggcacc accaccgcgt ccccggggat
     3181 gaaatttaac gatcatcacc cccccccttt ttccagcacc gcatgccgca gccgctccag
     3241 tcccgcgtcg attgccatca gggcggccac aatctgcacc cggtcatgac cggcaccacc
     3301 ggcattcact ttccgggcga tctggttcag gttgttaccc atacctgcaa gctggcgcag
     3361 cagcgccggg gatatagatg gcagtctgcc ggcacgcgcc ggcttctcat cgaggcaggt
     3421 ctgacgcatc cacgccgcca gctgcctgcc gtcgcaccgc tcgagcagcc gccggtgttc
     3481 gtcttccgtc acccacatcg tgagcatctt gttgcgtttg tctgccagag taacctcctg
     3541 ataacaccgg gacgggcggg aaatttttcc cgggcggcac ggtgttggac aagccgcagg
     3601 cgcgtcagtg gcttttagcg ggtttcgggg
//
