LOCUS       CP100010                4155 bp    DNA     circular BCT 21-JUL-2022
DEFINITION  Escherichia coli strain EC737A1 plasmid p737A1-4K, complete
            sequence.
ACCESSION   CP100010
VERSION     CP100010.1
DBLINK      BioProject: PRJNA827955
            BioSample: SAMN27625662
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Ni,J., Lin,J., Pan,D., Yang,H. and TANG,B.
  TITLE     cfr-harboring E. coli strains isolated from food animals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Tang,B., Chang,J., Wu,J., Yang,H., Lin,J., Guan,C., Jiang,H.,
            Chen,H. and Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
REFERENCE   3  (bases 1 to 4155)
  AUTHORS   Ni,J., Lin,J., Pan,D., Yang,H. and TANG,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. JAN-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/29/2022 21:57:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,772
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,434
            CDSs (with protein)               :: 4,434
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 73 of 219
            Pseudo Genes (incomplete)         :: 138 of 219
            Pseudo Genes (internal stop)      :: 50 of 219
            Pseudo Genes (multiple problems)  :: 37 of 219
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC737A1"
                     /isolation_source="gut"
                     /db_xref="taxon:562"
                     /plasmid="p737A1-4K"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2020-10"
     gene            join(3946..4155,1..222)
                     /locus_tag="MZH82_23815"
     CDS             join(3946..4155,1..222)
                     /locus_tag="MZH82_23815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UTU21869.1"
                     /translation="MSKTDCIMAFISLSNLAAKTGRIIQSQCVTATQTKKSTGAVELGF
                     RLSKSAMDETGITIGDRVDILYDKEENQWMIKKISEGGFGVSGQKNKHGGYSSCAVRLT
                     LRPGFPRLSEDEEDNKKLLSDDSFTQLSEGVIVFRLDDE"
                     /name="hypothetical protein"
     gene            480..1043
                     /locus_tag="MZH82_23820"
     CDS             480..1043
                     /locus_tag="MZH82_23820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UTU21870.1"
                     /translation="MHALSILPSVARANLATKFSSHLKVIELFNTMQDSQVVVLSSLIE
                     GHHLTSSGNSVKADFEVTRLPAIIEMLEKKYFFPIRHLNVSVKSVTTGRMTGQTVYFIE
                     PEHIEQLLADPELVFANQERSLFFRSLEKEGKNLGKLIEKKGSVSQAVLSLLHHAYKDK
                     PLSDEMWKEIEDKFTHMLDELSAA"
                     /name="hypothetical protein"
     gene            complement(1072..1698)
                     /locus_tag="MZH82_23825"
     CDS             complement(1072..1698)
                     /locus_tag="MZH82_23825"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="UTU21871.1"
                     /translation="MSPTLNKQASKERCAVEFATRKKSKTQRKKSKALSYRGLCFFCTL
                     VTGKCQWTSKMSKQDYIQLRTLTDQFYADNKGLQEALDGSNDGKVRGYGIVVIDLNGLV
                     FGIPLRSHLNHKFGFVSERSEGVKKGLDYTKALLIKKEEYVSRAYKIPTPEFTHINDNK
                     EKIQEDFNKFVNRYIEANVKKDENILRNYRYSTLKNYHKELGLED"
                     /name="hypothetical protein"
     gene            complement(2684..2878)
                     /locus_tag="MZH82_23830"
     CDS             complement(2684..2878)
                     /locus_tag="MZH82_23830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UTU21868.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQIRFEAESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          2916..3018
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.231"
                     /coverage="99.0"
                     /evalue="1.75e-35"
     Typing          3143..3398
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.86"
                     /coverage="89.0"
                     /evalue="1.25e-96"
     Typing          complement(1975..2488)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.023"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gaaaaccagt ggatgattaa gaaaattagt gaaggtggct ttggtgtgtc tgggcaaaaa
       61 aacaagcacg gcggttactc atcgtgtgct gttagattga ccttgcgacc aggttttcct
      121 cgcctttctg aggatgaaga ggacaacaag aagctacttt ccgatgattc attcactcag
      181 ctaagtgaag gtgtaatcgt cttcaggctg gatgatgaat aggaaggacc aaaaaggaaa
      241 ggcagagtta gtgagcgtcg ccaaacaatc gactaactcc gccttctggt caacacatca
      301 ctacctttgg aagggtggag tgttatgggc ggcattatgt tcataaactt gcattctaat
      361 aaagaaaaaa tcgtttcttt tgagggatgt gaacacgaca ttttctattc tgataggtgc
      421 gatgctattg gctttggtct cttaagttta tgtttttaaa aacatatgag gctatacata
      481 tgcacgcttt atcaatacta ccttcagttg cccgcgctaa cctggcaact aaattctcgt
      541 ctcatttgaa ggtgatcgag ctcttcaaca cgatgcagga ttcccaagtt gttgtcttgt
      601 catcactgat cgaaggccat cacctcacca gttctggtaa cagtgtcaaa gccgactttg
      661 aggtcactcg cttaccggcg attatcgaga tgttagagaa aaaatatttt ttccctattc
      721 gtcatctaaa tgtatctgtt aaatctgtaa ctacaggaag gatgactgga caaactgtct
      781 atttcataga gcctgaacac atcgagcaac ttctcgctga tcctgagctt gtgtttgcta
      841 atcaggaaag gagcctcttc tttcgttctc tcgaaaaaga agggaaaaat ctcggcaagc
      901 ttatcgagaa aaaaggtagt gtttctcaag ccgtcttaag ccttctacat cacgcctaca
      961 aagataaacc tctttctgac gagatgtgga aggagataga agataagttc acccatatgc
     1021 tggatgaatt gagcgcggcg taaaggcaag ggcggtcgtt agaccgcctt tttagtcttc
     1081 taaacctaat tctttgtgat agtttttcag cgtagagtaa cgatagtttc tgagtatgtt
     1141 ttcatctttc ttgacgttcg cttctatgta tctgtttacg aatttgttga aatcttcttg
     1201 tattttttct ttgttatcat tgatatgggt aaattcagga gttggtatct tataggctcg
     1261 cgatacatac tcttctttct taattagcaa cgctttggtg taatccagtc ctttcttaac
     1321 accttctgat cgctccgaaa cgaatccaaa tttatggttt aaatggcttc gtaagggtat
     1381 cccgaacacc aatccgttga gatcaataac tacgattccg tagcctctta cttttccatc
     1441 attactgccg tcgagagcct cttgtaggcc tttgttatca gcataaaact ggtctgtaag
     1501 ggttcttaac tgtatgtaat cctgtttgct catttttgat gtccattggc attttcctgt
     1561 aaccaatgta caaaaaaagc aaagccctct gtaagagagg gctttgcttt tctttcgttg
     1621 agtcttggat tttttacgtg tggcgaactc taccgcacat ctttctttgg aagcttgttt
     1681 gtttaacgtg gggctcattc ctaccgccac ttctagattt acttatatca aaaattatat
     1741 gtgaagtcaa tttcgtagct gttcttgact ggacttactt tgccagcgta atcttctcct
     1801 gaaacgaaaa aaccaccctg gcaggtggtt tttcgaaggt tatttaatcc tggcagatta
     1861 tctaaccgtg gtaacagtct tgtgcgagac gtgtcaccaa attcgtcctt ccagtgtagc
     1921 ctcagtaagg ccaccacttc aagaactctc aatacatctc tcgcacatca tgtttaccag
     1981 tggccgttgc caatggcgat tagtcgtgtc tttccgggtt ggactcaagt cgatagttac
     2041 cggaaaaggc gcagcggtcg gactgaacgg ggggttcgtg catacagtcc agcttggagc
     2101 gaactgccta cccggaactg agtgtcaggc gtggaatgag ataaacgcgg ccataacagc
     2161 ggaatgacac cggtaaaccg aaaggcagga acaggagagc gcacgaggga gccaccaggg
     2221 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc accactgatt tgagcgtcag
     2281 atttcgtgat gcttgtcagg ggggcggagc ctatggaaaa acggcagcgc cgcatccttt
     2341 tcttgccctg tttgtcatgc cgtatgcctt tgctcttatc cgtgtgcagg taacgcccgc
     2401 cgcagccgaa cggcaggagc ggaagcgacc gagtgagcga ggaggcgtaa tgtcaccgca
     2461 acctccatgt tctcccgtac gccctgcagc tgcattttcc cctgccacat gaagcacttt
     2521 tataaagtgt tactccgccg taacatatta agccagtata cactccgcta tcgctacgtg
     2581 actgggtcat ggctgcgccc cgacacccgc taaaaccccc tgacgggctt gtctgctgac
     2641 gaaaacgccc acgggaaagc cgtgaccgcc tccgtcagct tcgttatgtt cctgtttcac
     2701 tttcggcctc aaagcgaatc tggatgctgt tctggagttc ttctgcgagt tcgtgcagtc
     2761 gttcgcacat ggccgcctgt tcgtcggcat ccagagcatc cagtttttca agcagtgtca
     2821 ggctctggct tttgataaat cgcgccatat tcagcgcagt ttgttgttgt ttgttcacct
     2881 tccgttgttc tccgtcagtt ccgggtgtcg gggagaccct gagcaggtgg taattgtaag
     2941 acgttgcggg cgacggtatt acaattgcac atcctgtcct gtcctgtttc tttaggacgt
     3001 atcatggcac agcatatcaa ctgataaata atccctgtgc agtgcagccg ctgttgcata
     3061 aaatttcgct ctgcgtcgag tgtttcttaa tcaataattc cgctgatact ccggaaaaac
     3121 gcgagtctgt tctcatgcct gaaatgccca caccccacgc aaaaacaagt ttttgctgat
     3181 ttttctttat aaatagagag ttatgacaaa ttagttcttc ttgctctctt tgtgatattt
     3241 aaaaaagcgg tgttggcgca gctacaacac cgcgccgaca ccgcttttta ggggtggtac
     3301 tgactatttt cataaaaaac atcatcttat attaggggtg ctgctagcgg cgcggtgtgt
     3361 ttttttacag gacgcccctg ggggcgctgc caggggtgtc tgttcagata tgtagcaatg
     3421 agtcagggat tcacgaactg aaaatgtccg aaaatgtgtc tcatgggtac tgtatgcgcc
     3481 caaaccgatc gtcattcact ttcatgcata gctatgcagt gagctgaaat cgatcctgac
     3541 gcatttttcc ggtttacccc ggggaaaaca tctctttttg cggtgtttgc ggcagaatcg
     3601 cgttcagcgc gttttagcgg tgcgcgcaag aactcttatg ttaaattcat ctcatcggca
     3661 acctatacag gcataaaaat cagtttctat aagcggcttt cttgcaccta cttggtgatt
     3721 tttttggatg cgctgacgtt gctcgacttt aaatcaccat agaaaaatac cctttactta
     3781 cataaagatc gatctggatg tgtatcttga cgaggttcaa tacgagcaat tgtttttgta
     3841 tttaaggttt cttatcgccg ttttttgcgg ctctagaaaa atcaatacaa aagatcttga
     3901 aagtcacctc gctccctggg tacacttccg tataagaact gatcgatgtc taaaacggat
     3961 tgtattatgg cctttatctc tctatcgaac ttggcagcga agactggaag gataatccag
     4021 tcgcagtgtg ttacggccac gcaaacgaaa aaaagcacgg gcgctgtcga gctgggattc
     4081 cgcttgagca agagcgctat ggacgagact ggtattacca ttggggatcg tgtcgacatt
     4141 ctttacgaca aagaa
//
