LOCUS       CP100009                5268 bp    DNA     circular BCT 21-JUL-2022
DEFINITION  Escherichia coli strain EC737A1 plasmid p737A1-5K, complete
            sequence.
ACCESSION   CP100009
VERSION     CP100009.1
DBLINK      BioProject: PRJNA827955
            BioSample: SAMN27625662
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5268)
  AUTHORS   Ni,J., Lin,J., Pan,D., Yang,H. and TANG,B.
  TITLE     cfr-harboring E. coli strains isolated from food animals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5268)
  AUTHORS   Tang,B., Chang,J., Wu,J., Yang,H., Lin,J., Guan,C., Jiang,H.,
            Chen,H. and Li,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
REFERENCE   3  (bases 1 to 5268)
  AUTHORS   Ni,J., Lin,J., Pan,D., Yang,H. and TANG,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. JAN-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/29/2022 21:57:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,772
            CDSs (total)                      :: 4,653
            Genes (coding)                    :: 4,434
            CDSs (with protein)               :: 4,434
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 73 of 219
            Pseudo Genes (incomplete)         :: 138 of 219
            Pseudo Genes (internal stop)      :: 50 of 219
            Pseudo Genes (multiple problems)  :: 37 of 219
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC737A1"
                     /isolation_source="gut"
                     /db_xref="taxon:562"
                     /plasmid="p737A1-5K"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2020-10"
     gene            join(4696..5268,1..540)
                     /locus_tag="MZH82_23780"
     CDS             join(4696..5268,1..540)
                     /locus_tag="MZH82_23780"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="UTU21865.1"
                     /translation="MAKGLELAGFQHVGFVELNKHACDSLRLNFDEEKVFQGDIKNYDL
                     SSIDKIDIVAGGPPCQPFSLGGKHKAHDDSRDMFPFAIKAIEVLQPKAFIFENVKGLLR
                     KSFADYFEYIILRLTYPCLSLSNVSDWRIHLDALRKARKDKHCGCTYDVSFKLINAADY
                     GVPQIRERVIIVGVRSDLNKKWSFPSVTHTQEKLLWEQHVTGDYWRKHNIDAVIDKGIS
                     ERLMKRFGIFPPSGKSWVTVRDALKNVPQPYESHGIGDHIFKDGARVYPGHTGSYIDLP
                     SKTLKAGAHGVPGGENMIRYEDGTVRYFTVYEGKLLQTFPREFLIAGAWGEAMRQIGNA
                     VPVLLAQKIGQQLYNLLNEEVEPLLCSNFN"
                     /name="DNA cytosine methyltransferase"
     gene            complement(1602..1793)
                     /locus_tag="MZH82_23785"
     CDS             complement(1602..1793)
                     /locus_tag="MZH82_23785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="UTU21866.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1857..2180
                     /locus_tag="MZH82_23790"
     CDS             1857..2180
                     /locus_tag="MZH82_23790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="UTU21867.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2389..2820
                     /locus_tag="MZH82_23795"
                     /pseudo=""
     CDS             2389..2820
                     /locus_tag="MZH82_23795"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2858..3376
                     /locus_tag="MZH82_23800"
     CDS             2858..3376
                     /locus_tag="MZH82_23800"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="UTU21862.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQTNLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            3383..3616
                     /locus_tag="MZH82_23805"
     CDS             3383..3616
                     /locus_tag="MZH82_23805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="UTU21863.1"
                     /translation="MTELEIHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            4016..4660
                     /locus_tag="MZH82_23810"
     CDS             4016..4660
                     /locus_tag="MZH82_23810"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eco29kI family restriction endonuclease"
                     /protein_id="UTU21864.1"
                     /translation="MHNKKFDRSEHVYRNDSFLELIKDAVRFFSGTPVHSLPPPERFQG
                     AGVYALYYTGHYSLYDEYSRINRLAYNLPIYVGKAVPAGWRQSRISDHETRAGSELSNR
                     IREHSRNIAKTSNLNLCDFSCRFVIFEATGSDMISTVEAALIKIYKPLWNTVVDGFGNH
                     TPGAGRFAQAKSDWDVIHPGREWAEKCTGVHSEPYFIEERIKQYFSKSNFT"
                     /name="Eco29kI family restriction endonuclease"
     Typing          1432..1517
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="8.58e-38"
     Typing          complement(863..1407)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.011"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2171..3720
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.565"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 catactcaag aaaaattact gtgggaacag catgttactg gtgactattg gcgaaagcat
       61 aatattgatg cggttataga taaaggtatt tctgagcgtt taatgaaacg ttttggtatt
      121 ttccccccat cggggaaaag ctgggttact gttagggacg cattaaagaa tgttcctcaa
      181 ccatatgagt cacatggtat tggagatcat atctttaaag atggcgctcg agtttatcct
      241 ggtcatactg gaagttatat cgatttgcct tcaaaaacac ttaaggctgg cgctcatggc
      301 gtacctggag gtgaaaatat gattaggtat gaggatggga ctgttcgtta ttttacggtt
      361 tatgaaggga agcttcttca gacttttcct cgagagtttc tgattgcagg agcttggggg
      421 gaggctatga ggcagattgg taatgcagtt cctgtgttgc tagctcagaa gataggacag
      481 caattgtata acttattgaa tgaagaggtt gagcctttat tgtgctctaa cttcaattaa
      541 aagtttgtgt ctctatggtg gtgatacagc catagaggca atttgcaaaa aaagatcaaa
      601 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcctgtaaac aaaaaaacca
      661 ccgctaccaa cggtggttcg tttgccggaa tcaagagcta ccaactcttt ttcccgaagg
      721 taactggctt ggtggagcgc agataccaaa tactgtcctt ctagtgtagc ctcagttagg
      781 ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca atggctgctg
      841 ccagtggcgt taaggcgtgt cttaccgggt tggactcaag acgatagtta ccgggcaagg
      901 cgcagcagtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
      961 acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     1021 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     1081 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     1141 agcgtcgatt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcggcgt
     1201 ggccttttcc gggttcttgt tttttgctca catgtttctt ttggcgttat cccctgattc
     1261 tgtggataac cgcattaccg tctttgagtg agctgatacc gctcgccgca gccgaacgac
     1321 cgagcgtagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggc attttctcct
     1381 tacgcatctg tgcggtattt cacaccgtat atggtgctct tttcttatta tctgcactga
     1441 tgccgcatgg ttaagccagt atacactccg ctatcgctac gtgactgggt cagggctgcg
     1501 ccccgacacc cgctaaaacc tgctgacgcg ccctgacggg cttgtctgct cccggcattc
     1561 gctcacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     1621 tccccgaaac gtgcgaggca gctgcggtaa agctcatcgg cgtggtcgtg aagcgattca
     1681 caaatatcgg cctgttcatc tgcgtccagt tcgttgagct tctccagcag cgttaatgtc
     1741 tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttagt cactgatgcc
     1801 tccgtgtaag ggggatttct gttcatgggg taatgatacc gatgaaacgc gagaggatgc
     1861 tcacaatacg ggttactgat gatgaacatg cccggttact tgaacgctgt gagggtaaac
     1921 aactggcggt atggatgcgg cgggtctgcc tgggtgagcc ggttgcccgt tccggaaaac
     1981 tgccgacact ggcaccgccg ttactgcgtc agctggccgc catcggaaat aacctgaatc
     2041 agacagcccg taaggtgaac agcgggcagt ggtcttccgg tgaccgggtt caggtggtgg
     2101 ccgcactgat ggccatcggg gatgagctgc gccggctgcg tctggccgtc agggaacagg
     2161 ggacgcggga tgatagttaa gtttcatgcc aggggaaaag gtggtggcag tggtccggtt
     2221 gattacctgc tgggaaggga gcgtaaccgg gaaggggcaa cggtgcttca gggtaatccg
     2281 gaagaagtac gggaactcat cgatgccacg ccatttgcga agaaatacac gtccggtgtg
     2341 ctgtcgttcg cggagaagga gctgccaccg ggaggacgtg aaaaagtgat ggcgagcttt
     2401 gagcgtgtac tgatgcccgg tctcgaaaaa aatcagtaca gcatcctgtg ggtggaacac
     2461 caggacaagg gacggcttga gctgaatttt gtcattccga acatggagct acagaccgga
     2521 aaacgtctcc agccgtacta cgaccgcgca gacaggccga gaattgatgc ctggcagacg
     2581 ctggtgaatc accattacgg actgcatgac cccaacgccc cggagaaccg ccggacgctg
     2641 acccttcctg ataacctgcc ggaaacgaaa caggcacttg ctgagagcgt tacacgcggt
     2701 atagacgcac tttaccatgc cggagagata aaaggccgtc aggatgtgat tcaggcgctc
     2761 acagaggcgg ggcttgaagt ggtcagggtg acgcgaagca gtatcagcat tgcagacccg
     2821 aacggcggga agaatatcag gctgaaagga gcattttatg agcaatcttt tacagacggg
     2881 cgcggagttc gagaaaaagc tgaaagagag agccgaatct acagagaaaa tgctgaacga
     2941 cgagttcagg aagctcggcg agtctgtcag cgaggctgtg acctcaaacg agacgaaaat
     3001 cagagacgct atagccctgt tcacagcctc gacagagaaa tcgctggaaa aacaccggga
     3061 aggggtgaaa gaggcgatga tgcagcacag gaaggacgtg ttaaagctgg cagggaatac
     3121 gggcatgatg ttactgggga tagtctttct cctgtttacc gcgagtggcg ggacgctctg
     3181 gtatcttggc ggacggatac agacgaacct ggaagaaatc aggaagcagg aagagacatt
     3241 gcagaaactg aacgcgaaga catggggcgt ggagtttgtg caggacggga acaggaaatt
     3301 ccttgtcctt ccgtacggga aatcagcgga ggtgattccc tttcagggga aagagtgggt
     3361 acatctgaag gagtgacaca gagtgacaga gctggaaata catttgctga acgccttaga
     3421 gcagctgcaa caggactata tgcagcggct gagcgaatgg gagagcgcct tcgtggaatt
     3481 gcagaagatg ttttcgctta cgcaacggga caacgcgatg ctgaacgagc gggtcatgca
     3541 gttgagtcag caggtgcagc acttgagcga gcagacagaa cgcttgagcc agttatacag
     3601 cgagaactgg agataagaga ggaacggctg atacaggagc gcgaacatgc gttatccctg
     3661 gaacgggagc gtcagccgga aatacaggaa cgcacgctgg atggtccttc gctgggatgg
     3721 tgaaaccatg aaaaatggca gcttcagtgg attaagaggg gttaatgtgg cctgtaccat
     3781 ctggttgcat aggtattcat gcggttaaaa tttatcaggc gcgatcgcgg cagtttttcg
     3841 ggtggtttgt tgccattttt acctgtctgc tgccgtgatc gcgctgaacg cgttttagcg
     3901 gtgcgcgtaa tgcgacgtta tggtaaatgc gtatggtttt gggtatcagc tttatgatcg
     3961 tatcataggt gatgttgagt aggagtgtta atataaaaac ctttcggaaa acataatgca
     4021 caataagaaa tttgatagat ctgagcatgt ttatagaaat gactctttcc tagagttaat
     4081 aaaagatgcc gttcgttttt ttagtggtac gcctgttcat tcattaccac caccggaacg
     4141 gtttcaaggt gctggggtgt atgctcttta ctatacaggg cattattcat tatatgatga
     4201 atactctcgt ataaataggt tggcatataa ccttcctatt tatgttggca aggctgttcc
     4261 tgctggttgg cgtcagtcta gaattagtga tcatgaaact agggctggtt ctgaactatc
     4321 taatagaatt agagaacata gccgaaatat agcaaaaaca tcgaatctaa atttgtgtga
     4381 tttttcatgt cgctttgtta tatttgaggc tacggggtct gatatgatta gtacagttga
     4441 ggccgctctt attaaaattt ataagccttt gtggaataca gttgttgatg gctttggaaa
     4501 tcatacccct ggtgccgggc gatttgcgca agctaaatct gattgggatg taatacatcc
     4561 tggcagggag tgggctgaga aatgtacagg tgttcattct gagccatact ttattgagga
     4621 gagaattaaa cagtattttt ctaagagtaa ttttacataa tgagatcatt agagattttt
     4681 tcaggagccg gcggtttggc taaaggtcta gagttggcag gatttcagca tgtaggattt
     4741 gttgagttaa acaagcatgc atgtgattct ttgcgactta attttgatga ggagaaggta
     4801 tttcagggag atattaaaaa ttatgacctt tcatctatag ataaaataga tattgttgct
     4861 ggcgggcctc cctgccaacc attttcattg ggcggaaaac ataaagctca tgatgactct
     4921 agggatatgt tcccatttgc aataaaagca attgaagtat tgcaaccaaa agcttttatt
     4981 tttgaaaatg ttaaagggtt gcttagaaaa tcatttgcgg attattttga atacataatt
     5041 cttcgtttga cttatccttg tttatcgtta agtaatgtaa gtgattggag aatacatcta
     5101 gatgcattaa gaaaagctcg caaggataaa cactgtgggt gtacatatga tgtcagtttt
     5161 aaattgatca atgcagctga ttatggagtt ccacaaatta gggaacgggt cataattgtt
     5221 ggtgttcgaa gtgatttgaa taaaaaatgg tcttttccaa gcgtaacg
//
