LOCUS       CP099290                2460 bp    DNA     circular BCT 13-APR-2023
DEFINITION  Escherichia coli strain RHB03-E1-C04 plasmid unnamed3, complete
            sequence.
ACCESSION   CP099290
VERSION     CP099290.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15488496
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB03-E1-C04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 135x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/15/2022 20:04:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,369
            CDSs (total)                      :: 4,250
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 228
            CDSs (without protein)            :: 228
            Pseudo Genes (ambiguous residues) :: 0 of 228
            Pseudo Genes (frameshifted)       :: 66 of 228
            Pseudo Genes (incomplete)         :: 151 of 228
            Pseudo Genes (internal stop)      :: 48 of 228
            Pseudo Genes (multiple problems)  :: 35 of 228
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB03-E1-C04"
                     /isolation_source="Pooled sediment sample collected from
                     floor of pig farm"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            522..1538
                     /locus_tag="NFJ32_21835"
     CDS             522..1538
                     /locus_tag="NFJ32_21835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001707479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WFV43444.1"
                     /translation="MRSPDWLDVRQNEGVSMANYAIMRCKKLTGMGSVASALQHCYRER
                     ETPNADAERTPENYCSVSQSADEAMGKLRELLPEKRRKDAVLAVEYVMTASPEWWNEAT
                     PRQQAEFFARSEQWLEKKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGG
                     RSKMREDQSTYAESVKKLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPR
                     VLRKGIFTKDVEDQAAIAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKR
                     LQSVTEPFKGLSREQMTEILMMAQRFKQKNQEKEKQQRIEREKQRQTRSRGMGGMER"
                     /name="plasmid recombination protein"
     gene            complement(1637..1867)
                     /locus_tag="NFJ32_21840"
     CDS             complement(1637..1867)
                     /locus_tag="NFJ32_21840"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WFV43442.1"
                     /translation="MMNAEDKLFKNVDRLMLHDSLKKNEVLTVWERTFLSSVYSIYRRT
                     SGNKYSTKGLSPKQKNAAFSILKKYNYHLMD"
                     /name="hypothetical protein"
     gene            complement(1854..2294)
                     /locus_tag="NFJ32_21845"
     CDS             complement(1854..2294)
                     /locus_tag="NFJ32_21845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WFV43443.1"
                     /translation="MDENGEVKQDSKIETFSIPAEPPYVKLYIADIAMIHGLSKSQNDV
                     LLQLAMLIGWDGFVSVSKTRFEKTIQPRVHIEYQTFKNIIAALVDKQIFFRAGRGELEA
                     NPFLFARGDWPSIYERRKKIELKISYDTNGRTVQTKVMDDEC"
                     /name="hypothetical protein"
     Typing          523..1535
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.041"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acgattagta cttgaggtga gtacgattag tccttgaggt gagtacgatt cttcgctcta
       61 gcccttggta caagcgggtt cccatatcta tatctatata ttaagaggga aaataaaggc
      121 tgaaaaacgc catttttaaa catcgaaaaa cgagcttttt tgagtctgga gcgtacggat
      181 ttctatgtcc ggggagtgga aattccgctt cctcgaacgg acagactcgc ggcgatgccg
      241 ctccctaatc cgttcttgcg gcgagcggtt cctgctcttt caaaaacgat attcattgcc
      301 tgatttcgtc aggggcgagc gaagcgagtg ccatttaccc ttgacgactc accggataaa
      361 acaggcttgg cagtggtggg gggtggcgcg gagcgacgcc ttccacctgc cagactgccg
      421 ttgattttga ccttctggca gcttgctgcc cgaacgattt tgcgtagcaa agtctagtga
      481 gtacactatc atcctgatag tggcccgccg gaggcatcgc aatgcgatcc cccgactggc
      541 tggatgtacg ccagaacgag ggggtaagca tggcgaatta cgcgataatg cgctgcaaaa
      601 agctgacggg aatgggcagt gtggccagcg ctttgcagca ctgctacagg gagcgtgaga
      661 cgccgaatgc tgacgctgaa cggacaccag aaaactactg ctctgtgtct cagtcggcag
      721 atgaggcgat gggaaagctg cgggagctgt tgccggaaaa acgccgtaaa gatgcggtac
      781 tggctgttga atacgttatg acggcaagcc ctgagtggtg gaacgaggcc acaccgcggc
      841 agcaggcgga gtttttcgcc cgctctgagc aatggctgga gaagaaatac gggaaggatc
      901 gtgttgtggc ggcggtggtt caccgggacg aggccacccc gcacctgtct gcgttcgtgg
      961 tgccattgac gcaggatgga cggctgtccg cgaaagagtt tattggcggg cggtcaaaga
     1021 tgcgtgaaga tcagagcacc tacgctgaat cagtaaaaaa actgggtctt gagcgcggca
     1081 tcgagggaag ccgcgccacg catcagacag ttcagcacta ctacgaatcg ataaaccgtg
     1141 gcacccgtag ccaggtatcg atctcgccgg aggcgctgga gccgcgtgta ttgcggaagg
     1201 gcattttcac caaagacgtg gaagatcagg cagccatagc caaacgtcta agccacgcag
     1261 tgaacgatgg ctttgcagga accatcgcta tggcctctca gagcgcccag aacgcaaaaa
     1321 gagcgcgtga gctacagaag actatggatg cgcagcagaa acgcctacag agcgttacag
     1381 agccttttaa ggggctttcc agagagcaga tgaccgaaat cctgatgatg gctcagcggt
     1441 ttaagcagaa aaaccaggaa aaggaaaaac agcagcgtat tgaacgtgaa aaacagcgac
     1501 agacgcgatc gcgtggcatg ggtggtatgg agcgttagtt ttgatgcgat gcaggatagg
     1561 gctaacgccg caaagtgacg tcatttgcgg cattccagag atcaggttct gccgccctgt
     1621 ataacgctga ggccagctaa tccatcagat ggtaattgta ttttttcaaa atagaaaaag
     1681 ctgcgttttt ctgctttggt gacaaaccct tagttgagta tttattaccg ctagttctac
     1741 gatatatgct gtaaacactg cttaaaaaag tacgctccca aacagtcaga acctcattct
     1801 tttttagtga atcatgcagc attagcctgt ctacattttt aaaaagttta tcttcagcat
     1861 tcatcatcca ttaccttcgt ctgcaccgtt cttccattgg tgtcataact gatctttaac
     1921 tcaattttct ttcttctttc gtaaattgat ggccagtctc cccgtgcaaa aagaaagggg
     1981 ttagcttcaa gttccccacg ccctgcccgg aagaatattt gtttatctac aagagccgca
     2041 attatgtttt taaaggtctg atattcaata tgaaccctgg gctgtatggt tttttcaaat
     2101 ctagtttttg atacagacac aaacccatcc catccaatca gcattgccaa ttgtaaaagc
     2161 acatcatttt gagattttga taaaccatga atcatcgcaa tgtctgcaat gtatagcttg
     2221 acgtagggag gttcggctgg gatggaaaag gtctcgatct tagagtcttg ttttacctca
     2281 ccgttttcat ccatgtatgt atgccgttca agcctagaaa tctttttcgt attcactcca
     2341 gccccacgtt tcgccatgat ttagtactcg catttaagta ctaaagagac atacagtcaa
     2401 gtcctttgca tcaagtacga ttagtacttg aggtgagtac gattagtcct tgaggtgagt
//
