LOCUS       CP099289                8136 bp    DNA     circular BCT 13-APR-2023
DEFINITION  Escherichia coli strain RHB03-E1-C04 plasmid unnamed2, complete
            sequence.
ACCESSION   CP099289
VERSION     CP099289.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15488496
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8136)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8136)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB03-E1-C04
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 135x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/15/2022 20:04:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,369
            CDSs (total)                      :: 4,250
            Genes (coding)                    :: 4,022
            CDSs (with protein)               :: 4,022
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 228
            CDSs (without protein)            :: 228
            Pseudo Genes (ambiguous residues) :: 0 of 228
            Pseudo Genes (frameshifted)       :: 66 of 228
            Pseudo Genes (incomplete)         :: 151 of 228
            Pseudo Genes (internal stop)      :: 48 of 228
            Pseudo Genes (multiple problems)  :: 35 of 228
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB03-E1-C04"
                     /isolation_source="Pooled sediment sample collected from
                     floor of pig farm"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(7402..8136,1..249)
                     /locus_tag="NFJ32_21785"
     CDS             join(7402..8136,1..249)
                     /locus_tag="NFJ32_21785"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="WFV43439.1"
                     /translation="MKKKFSIIVPVYNVEKYLRECIQSVLDQDFNDYEIICVNDGSTDG
                     SLDIILSFDDQRIKVINQENQGQSVARNVAIESSTGEYIWFLDGDDKISHGSLKLIDKI
                     TNEKNYGIIFFNAHVFFDTPDLKKKGWHDYKRSTFGEIDALTFFEKEMANKKLIVSPCC
                     YVFKRELLNGNAFIPHIYHEDNHFIATLIFDNYSTICLSLDEELFLRRVRNDSVMTSKK
                     SMRHIIGYSTCIDELTKYKKIKGIQNKSFDRFVNGMVLSLFSISETIKTKEAMNYVARY
                     KKHPGLSFKDSVSINYPLIKKTYLKLKNGKVFNYLRNGIINKKSNP"
                     /name="glycosyltransferase family 2 protein"
     gene            complement(708..1411)
                     /locus_tag="NFJ32_21790"
     CDS             complement(join(708..1162,1162..1411))
                     /locus_tag="NFJ32_21790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /ribosomal_slippage=""
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology. GO_function: GO:0004803 - transposase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WFV43440.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLNLRQHLARLGRKSLSFSKSVELHDK
                     VIGHYLNIKHYQ"
                     /name="IS1 family transposase"
     gene            1630..3114
                     /locus_tag="NFJ32_21795"
     CDS             1630..3114
                     /locus_tag="NFJ32_21795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="WFV43441.1"
                     /translation="MKIQKDIKNNMTALVSSFKWVGIGQLVKIATQLVSLFYLTHLIPP
                     SSYGLLAMSSVVMNLASIFNDMGTGAAVIQRQEASIGFYNYIYRINIFTGLFVMLLVII
                     TSPLIVVYFNTPELYPVLFLLAFSFPISSLTITHKAKLEKEMNFKVIIAIEIFSSLTGM
                     VLAIVLANLGFGVYSLIFQTLTTISISTICFLKVSGLSLSLRIKNISHSDKSGVIGFSG
                     YLLSFNLINYFSRNLDSILIGRFFSSAILGVYSIAYRIMLFPLQSLTLVFSRVFLPHLS
                     KNINNTNNTNKQDYLNALKLILSLSAPLMLGLSATSFSFTRIFFDPKWHLLGDLLHWLA
                     PTAIIQSALSTTGSVFMAYGKTRWLFFLGCIGAFLMGLSFIIGVHFDIKTLVYLYFFAN
                     LINSLPAFILLKNILKFSYIELVNVFFRTISPAFLMFLLIKVWQHFSFFKNASIDFSFN
                     VALGIFSYIVFYTLFNFNEVVRTGVIHKLRCMTKLK"
                     /name="lipopolysaccharide biosynthesis protein"
     gene            complement(3207..3474)
                     /locus_tag="NFJ32_21800"
                     /pseudo=""
     CDS             complement(3207..3474)
                     /locus_tag="NFJ32_21800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558268.1"
                     /note="frameshifted; internal stop; incomplete; partial on
                     complete genome; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element transposase"
                     /name="IS1-like element transposase"
     gene            complement(3446..3911)
                     /locus_tag="NFJ32_21805"
                     /pseudo=""
     CDS             complement(3446..3911)
                     /locus_tag="NFJ32_21805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517860.1"
                     /note="frameshifted; incomplete; partial on complete
                     genome; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(4110..4394)
                     /locus_tag="NFJ32_21810"
     CDS             complement(4110..4394)
                     /locus_tag="NFJ32_21810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WFV43435.1"
                     /translation="MSFFDELKASLEEAVEIKNGTKAPARVTRYELADVRAIRENLNIT
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFDELAAH"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(4396..4482)
                     /locus_tag="NFJ32_21815"
                     /pseudo=""
     CDS             complement(4396..4482)
                     /locus_tag="NFJ32_21815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin RelE"
                     /name="addiction module toxin RelE"
     gene            complement(5015..5332)
                     /locus_tag="NFJ32_21820"
     CDS             complement(5015..5332)
                     /locus_tag="NFJ32_21820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WFV43436.1"
                     /translation="MKKIFILSAALIMAGCATKQYPQAVNVTGPESSAMDCKAVALEIA
                     KTRSVQQEIEETGEFDGRTVLGFLGDFGIGNGMAKGEARKKAANRLNQLEALKAAKCAD
                     Q"
                     /name="hypothetical protein"
     gene            6417..6566
                     /locus_tag="NFJ32_21825"
     CDS             6417..6566
                     /locus_tag="NFJ32_21825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WFV43437.1"
                     /translation="MPSEHDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            6949..7317
                     /locus_tag="NFJ32_21830"
     CDS             6949..7317
                     /locus_tag="NFJ32_21830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WFV43438.1"
                     /translation="MANFNPGPRTAIAAVRDSKNDIETWKKNGIRREEIVTVLNERYGL
                     NISLVAFDKALYRLRKSTDEPSLADVDSVSAAETNFSEASGPDNGFFLSGNDFMRAEEE
                     SQRVVESFRDSLSRKKGF"
                     /name="hypothetical protein"
     Typing          6209..6382
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.571"
                     /coverage="100.0"
                     /evalue="2.6e-76"
     Typing          complement(5734..6242)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.51"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 atacaaaaca agagctttga tagatttgtg aatggaatgg ttttgtcgtt attctctata
       61 tcggaaacaa taaaaacaaa ggaagcaatg aactatgtgg cgagatataa aaaacacccc
      121 ggcctatcat ttaaagatag cgtatcaatt aattacccat taattaaaaa gacttattta
      181 aaactaaaaa acgggaaggt gtttaattac ctaaggaatg gcattattaa taaaaaaagc
      241 aacccttaat aaaagcaaag cgataattaa taaaggtttt tctttgttga ataaataaga
      301 tttcggatag gtatccccgt gacgggttgg gttttcaggc gatacgcacg ctttccgggg
      361 ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc agatcggggt cagtttggag
      421 atcggaaatt tttgtacgtt aagcgcatta tttaaccaat cttggttcgt aaggggttga
      481 ttttgcgtca gtggctatcg cttttaagtt gtcgctcttg cagtatcgca tttaggttat
      541 ccgattaagg tcaaacctct gaaaatgtcg tatagcgcgg gagtacaccc tattgctata
      601 ggtaagtctg ttcaaaaaac aggcttaccg tacaataatt ctctatatcc aaactgaccc
      661 cagatcacgc atcttcccga caacgcagac cgggtaatga ctccaactta ctgatagtgt
      721 tttatgttca gataatgccc gatgaccttg tcatgcagct ccaccgattt tgagaacgac
      781 agtgacttcc gtcccagcct tgccagatgt tgtctcagat tcagattcag attatgtcgc
      841 tcaatgcgct gagtgtaacg cttgctgata acgtgcagct ttcccttcag gcgtgattca
      901 tacagcggcc agccatccgt catccatacc acgacctcaa aggccgacag caggctcaga
      961 agacgctcca gtgtggccag agtgcgttca ccgaagacgt gcgccacaac cgtccttcgt
     1021 atcctgtcat acgcgtaaaa cagccagcgc tgacgtgatt tagcaccgac gtagccccac
     1081 tgttcgtcca tttcagcgca gacaatcaca tcactgcccg gttgtatgcg cgaggttacc
     1141 gactgcggcc tgagtttttt aagtgacgta aaaccgtgtt gaggccaacg cccataatgc
     1201 gtgcactggc gcgacatccg acgccattca tggccatatc aatgattttc tggtgcgtac
     1261 cgggctgaga ggcggtgtaa gtgaactgta gttgccatgt tttacggcaa tgagagcaga
     1321 gatagcgctg atgtccggca gtgcttttgc cgttacgcac cacgccttca gtagcggagc
     1381 aggaaggaca tctgatggaa atggaagcca cgcaagcacc ttaaaattac catcatacac
     1441 taaatcagta agttggcagc attacccact attggtggag atgatcataa tgccttataa
     1501 tcatacggga tacgaactta taaagtccta aagtttagct aaatcggaat ctacaacgta
     1561 gatgatagct tgaagaatta tgatgtcttt tatggtggta tctttaaaac catcacggat
     1621 tataaattat tgaaaattca aaaggatatt aaaaacaata tgactgcact agtcagtagt
     1681 tttaaatggg ttggcattgg acaacttgta aaaatagcaa cacaattggt ttcgttattc
     1741 tacttgacgc acttaatccc cccatcgagc tatggactat tagcaatgtc atctgtggtt
     1801 atgaatttag caagcatatt caatgacatg ggaactgggg cggcagtaat tcagcgacaa
     1861 gaggcgtcga tcggttttta taattacata tacaggataa acatttttac tgggttgttt
     1921 gttatgctgc ttgtaataat aacatcccca ttaattgtcg tgtactttaa cacacccgag
     1981 ctatacccgg ttctatttct attggctttt agctttccaa tttccagctt aacaataact
     2041 cacaaagcaa aattagaaaa agaaatgaac tttaaagtca tcattgcaat tgaaattttc
     2101 tctagcctta caggaatggt gctggcaata gttttagcta atttaggctt tggtgtttat
     2161 agtttgattt ttcaaacact gacaacaata tcaatatcca caatatgttt tttgaaggtt
     2221 tccgggttga gtttatcatt gagaattaaa aatatcagcc atagtgacaa gtcaggagtt
     2281 atagggttta gtggttattt attatctttt aatttaataa attatttttc caggaatctt
     2341 gattctattt taataggccg ttttttctca tccgcaatac ttggggtcta ttcgattgcc
     2401 taccgcatta tgttatttcc gttgcagagc ctgacgcttg ttttttctag agttttctta
     2461 ccacacctta gcaaaaacat aaataacact aataacacta ataaacaaga ttacttaaat
     2521 gcattaaaac ttatactatc attaagcgcg ccactgatgc taggtttatc tgctacatct
     2581 ttttctttta cgaggatttt ctttgatcct aagtggcacc tacttggaga cttattgcat
     2641 tggttggcac ctacagcgat tatccaatca gccttgagta caacaggttc tgtttttatg
     2701 gcctacggaa aaacaaggtg gttgtttttc ttaggttgca ttggcgcttt tttgatgggg
     2761 ttgtctttca ttataggtgt tcatttcgat ataaaaacat tggtctacct ttattttttt
     2821 gcaaacttaa ttaattcact cccggcattt attttactta agaatatctt gaagtttagt
     2881 tacattgaat tggtaaacgt gttttttaga actatctccc cagctttttt gatgttctta
     2941 cttattaaag tatggcagca tttttccttt ttcaaaaatg ccagcattga tttttctttt
     3001 aatgtcgcat tgggtatttt tagttatatt gttttttata ccttgtttaa tttcaatgag
     3061 gttgttagga caggagtaat tcataagtta agatgcatga ccaagttaaa gtgattcatt
     3121 tattaaattc attaaatgaa ttggtaatga ctccaactta ctgcttgtgt tttatgttca
     3181 gataatgccc gataccgact gcggcctaat ttttttaagt gtcgtaaaat cgtgttgagg
     3241 acagtgccca taatgcggga tgtggcccgg catccaacgc cattcatggc catatcaatg
     3301 attttctggt gtgtaccggg ctcagaggcg gtgtaagtga actgtagttg cttgctatgt
     3361 tttacgacag tgagagcaga gatagcgttg atgtccggta gtgcttttac cgttacgcac
     3421 cacgccttca gtagctgaac aggagggata accagtccag cgtgtagagt tcaggctgcc
     3481 atacgggcaa ttttccagtg actgtggttc ggattcttgc aaattagccc cgaatacccc
     3541 gtgtcagcgc ccaatttttt acgtagcgcg ctctctacag cagcggcgta acgtaacggt
     3601 ttcatttttc cgtctggcgc ggtgcgtatc gaggttttca gtgcgtacag caggtgcgcg
     3661 tgtccgtttt cggggttggt gatggtcagt gtcggcgcgg gggcgttgcg gtcgctccag
     3721 tcaatggctg cgccgatgcg gtcaacgtca aatcccagcc agaacatggc gtgaggctga
     3781 ttaaactgga tgtatttagc cagaatagcg cgctctttcc ctgcgatgcg gacaccaaag
     3841 tgtaaatcat ccgagaagta cggtttatgc ggcaagcggt cgttgaaaag ttcgagtgct
     3901 gcgttacgca atgtgccctg aacttatggc gggctgttgt ctggcacggt tcatgtgcta
     3961 atatccttct taggttttat acctagccct gatttatcat gtctcttccg ccaagttgaa
     4021 atcatgatta tcggggcttt gttttttctg ttgccgttaa agttacacgc gaacggcctc
     4081 gcctgtcgaa tttcttctgt cacatcagct taatgcgccg ccagttcatc aaaaaacttt
     4141 gggttagcct gaatggtggc cagcactttt gctgccagtc cggttgggtt acggcgtccg
     4201 gtttcccagc ttttgatggt atcgaggctg gttcccagca ctttcgccat ttcgctctga
     4261 gtaatattca ggttttctct gatagccctg acgtctgcca gctcgtagcg ggtcacacgg
     4321 gcgggggctt tggttccgtt tttgatttca acggcctctt ccagagaggc tttcagctcg
     4381 tcaaaaaaac tcatcttaca cctcgtcttt cagtcgttgg gtcagcttct tcagttctgc
     4441 tttctctgcg tctgtgatgc tgtctttggt gcttttaggg tacgtacagc atgtagacaa
     4501 catgtccgaa agtaagcaaa accattaacg gacagccgga tcatttcgta catcccttac
     4561 ggtcaggggt tcgcgctcaa tggaacgaaa tcacccgtta gcttaaaacg cttaacgtag
     4621 gataatttcc tttttccaag cggacccgct ctactcgaaa ttgtcggata cctgcaatta
     4681 cctaccgtgc cttacggctt ggtcaatgcc tcacggccac gcaataaatt gcgtccccgt
     4741 tcaccgtttt cggataagaa gaacatcgga aacaggactt accccggctg aatggcgtga
     4801 atttccgcgc tatgcacttg cgcgcatact catgcatgcc gtaaaaacag agcctgcgcg
     4861 tttctggcgg gttttcgggt ggtttgttgc ctgttttacc ggttttccgt cagaaacgcg
     4921 ctgagggcct ctgaggcggt gcggccaacg gatgttatgg taaattcagc tgattggcag
     4981 gtacaaaaaa agccccgcag ggcttttttg taccttattg atctgcacac tttgcagcct
     5041 tcagggcttc cagctgattt agccggttag ctgctttttt gcgggcttcg ccttttgcca
     5101 tgccatttcc tatcccaaaa tcacccagga aacctaatac ggtgcgcccg tcaaattcgc
     5161 cggtttcctc aatttcttgt tgtacactgc gcgttttggc gatttccaga gctaccgctt
     5221 tgcaatccat tgcggaagat tctggaccgg tgacgttcac ggcctgtgga tattgctttg
     5281 ttgcgcatcc tgccatgatc agcgctgccg ataggatgaa tatttttttc aaaattatcc
     5341 ttatttattt aggcatagtt aagagatacc cattttatcg gcaggaaatg agggtttttt
     5401 agttgaattt taggaggggc aaggttccat attggtcgag gattctggag ctgaccggca
     5461 acggccggcg cggctatggg ccatgcggtg gttgagcata gaaaggatct tcttgagttc
     5521 ctttttttct acgcgtaatc tcttgctctg aaaacgaaaa aaccacctgg ggaggtggtt
     5581 tttcgaaggt taagtcagtt ggggaactgc ttaacctggt aactggcttc agcagagcgc
     5641 agataccaaa tactgtcctt ccagtgtagc cgtagttagg ccaccacttc aagaactcta
     5701 tagcaccacc tacattaact cgctctgcta atcctgttac cagtggctgc tgccagtggc
     5761 gctttgtcgt gtctttccgg gttggactca agacgatagt taccggataa ggcgcagcag
     5821 tcggactgaa cggggggttc gtgcatacag tccagcttgg agcgaactgc cttcccggaa
     5881 ctgagtgtca ggcgtggaat gagacaaacg cggccataac agcggaatga caccggtaaa
     5941 ccgaaaggca ggaacaggag agcgcacgag ggagccgcca gggggaaacg cctggtatct
     6001 ttatagtcct gtcgggtttc gccaccactg atttgagcgt cagatttcgt gatgcttgtc
     6061 aggggggcgg agcctatgga aaaacggctt tgccgcggcc ctatcgcttc cctgttaagt
     6121 atttccctgg catcttccag gacatctccg ccccgttcgt aagccatttc cgctcgccgc
     6181 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaatatat cctgtatcac
     6241 atattctgct gacgcaccgg tgccgccttt tttgtcctgc tacatgaagc acttcactga
     6301 ctccctcatc agtgccaaca tagtaagcca gtatacactc cgctagcgct acgtgactgg
     6361 ttcagggctg cgccccgaaa ccctctaaca acctctcagc tactggatat caccccatgc
     6421 catctgaaca tgatgaacaa ctggattcga ctgatacgca gcttcccggc gaatatgacc
     6481 tttttgattt aatggcgcag gttactgaag agaacctgca tgccccggtg gattttgggc
     6541 cgcctgtcgg ccgggaagcg ttatagcttt ttcgcttacc cgcgtacgtg gtaatacgcc
     6601 ggtattacat cttatttctt tattttcatg taattacagg tggttgtaat acgttgcgta
     6661 ttacataaat aaacggggac ttcagttttt atcttttaat tcatgcaatt gcgatgaaaa
     6721 tcgcggttgc gtatggtgta tgaccattta agggataatg taacctgact tttaaagaat
     6781 gaatctatct ttggcagcgc atgagataga taaaaagcgt tccacatcag gcggttcatg
     6841 tatccatgaa ccggaaagga caaaaataag acaattgtaa gacatttttt gcgttttact
     6901 cttattttgg actattatga gacaaatgta agacattatg ggcggtatat ggcgaatttt
     6961 aaccctggtc caagaacagc cattgcagct gtcagggatt ccaagaacga tatagaaacc
     7021 tggaagaaaa atggtattcg tagggaagag attgtgactg tgctgaatga acgttacggc
     7081 ctgaatatct cactggttgc gtttgataag gctctttaca gattgagaaa aagtacagat
     7141 gaaccgtctc ttgctgatgt ggattctgtg tctgctgcag aaacgaattt ctctgaagcc
     7201 agtgggcctg acaatggttt ttttctgtcc gggaatgact tcatgcgagc agaagaagaa
     7261 tctcaaagag tggttgagtc attcagagat agccttagca ggaagaaagg attctgattt
     7321 attcaacaaa gccgttgata aatcgcattc cataaataac aacttgatgt tagtataact
     7381 aaatattatt tggagattac tgtgaaaaaa aaattttcga taatagtacc tgtatataac
     7441 gttgaaaaat acttaaggga atgtattcaa tcagtattag atcaggattt taacgactat
     7501 gagataatct gtgtaaatga tggttcaaca gatggcagtt tagatatcat actgtcattt
     7561 gatgaccaaa gaatcaaagt aataaatcag gaaaatcaag gccaatcggt ggcaagaaat
     7621 gtcgccattg aatcatcaac tggtgaatat atatggtttt tagatggtga tgataaaata
     7681 tcacatggaa gtctaaagtt aatagacaag ataaccaatg aaaaaaatta tgggattatt
     7741 ttctttaatg cacatgtttt ttttgacact ccagatctga aaaaaaaagg ctggcatgat
     7801 tataagcgct caacatttgg cgaaatagat gcattaactt tctttgaaaa agaaatggcg
     7861 aacaaaaaac taatagtgtc accatgttgc tatgttttta aaagggagtt attaaatggc
     7921 aatgcattca ttccgcatat atatcatgag gataatcatt ttatagcaac cctgattttt
     7981 gacaattatt caactatatg cctctcgttg gatgaggagt tatttcttag gagggtaaga
     8041 aatgattccg taatgacgtc taaaaaatca atgaggcaca tcattggata ttcaacatgc
     8101 atcgatgagc ttacgaagta taaaaaaata aaaggc
//
