LOCUS       CP099276                5145 bp    DNA     circular BCT 13-APR-2023
DEFINITION  Escherichia coli strain RHB05-SO-C07 plasmid unnamed1, complete
            sequence.
ACCESSION   CP099276
VERSION     CP099276.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15488508
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5145)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB05-SO-C07
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 114x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/15/2022 19:52:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,543
            CDSs (total)                      :: 4,427
            Genes (coding)                    :: 4,350
            CDSs (with protein)               :: 4,350
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 49 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 10 of 77
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB05-SO-C07"
                     /isolation_source="Pooled soil sample collected from floor
                     of sheep farm"
                     /db_xref="taxon:562"
                     /plasmid="unnamed1"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(4953..5145,1..17)
                     /locus_tag="NFJ44_22655"
     CDS             join(4953..5145,1..17)
                     /locus_tag="NFJ44_22655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WFV70110.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLRKLPPDHTIPPDLTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     gene            complement(83..616)
                     /locus_tag="NFJ44_22660"
     CDS             complement(83..616)
                     /locus_tag="NFJ44_22660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WFV70111.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISLDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTMLDRNYNATAAVYQKVVTLED
                     KLAEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(619..1545)
                     /locus_tag="NFJ44_22665"
     CDS             complement(619..1545)
                     /locus_tag="NFJ44_22665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WFV70112.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPDRGGAKKDSGNLT
                     PTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYYRHLEQARMVGHDIREWLDTPVKQHELVRRLQHDIAREHGFD
                     MRDGVSRQNRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            1722..2120
                     /locus_tag="NFJ44_22670"
     CDS             1722..2120
                     /locus_tag="NFJ44_22670"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WFV70113.1"
                     /translation="MRISNIEWLKKRIEFIRKLGKQTARQRQIIDLLDNEDRLTEQERK
                     LLHVLATAEKNDLQAQESERKQAIQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDMLLQRLKPIKYDLDTCPNPDA"
                     /name="hypothetical protein"
     gene            complement(2313..3221)
                     /locus_tag="NFJ44_22675"
     CDS             complement(2313..3221)
                     /locus_tag="NFJ44_22675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009440807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WFV70114.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPEFSQWLDAVIQRVEMYNASLPVPLSP
                     AECRAIGRSIAKYTHRKFSPEGFSAVQAARGRKGGIAKGEAYEDKRFMALCMLENGYSQ
                     KAIAAMLNVHRNTIRNWAMHK"
                     /name="replication initiation protein"
     Typing          complement(2494..3221)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.61"
                     /coverage="88.0"
                     /evalue="0.0"
     Typing          complement(623..1545)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agacagaatc atattgaaaa aaacggtgaa cggttcgcga aatcctttcg cgctccgtga
       61 ggcgttggac aagcgaagcg cgtcagtttt tccggctctt acccgtgaca ggtttctccg
      121 ccagtttatc ttccagtgtc acaactttct gatacaccgc cgctgtggcg ttgtagttcc
      181 tgtcgagcat tgttttcatt ccccatccga gcacacagaa cataaccaga aaagccactg
      241 acacaccacc cagccatttc cagctatacc ctttaagggt gttgtattcc tgcacgatat
      301 tgctgtgaat tgtcgcaata tccctgttca gggcatccgc gtttgccttc atgtttgcga
      361 ctgactcttc cagccgctga aaatccaggc tgatgcgctc acctatcagc cctgctacgt
      421 ccagcttctg cacctcattt ctgacttcat ccctgacctg ttttgccaca ccgtcagaaa
      481 tatcccgata tccgcgaagc gtttcccttg ccacagccag agaatcagca acagctttct
      541 gttgctccat tgcggtatgt tgcagatgct ccaccgataa caggagcgtg agtaattttt
      601 cttctgtgct ggccatgttc agaatcccat agaaaatcca cgtgacggtg atttaacccg
      661 ttcaggttca gttcggttct gtctgctcac tccgtctctc atgtcaaagc catgttcccg
      721 cgcaatatca tgttgcagcc ttctgaccag ttcatgttgc ttaaccggtg tatccagcca
      781 ttccctgatg tcatgcccga ccattcgcgc ctgttcaaga tggcggtaat agtccggttc
      841 agaccgtttt tgcatgatgt catgacgctg ggcatcatca agtcgtccgg cagcttcaaa
      901 acccagatgc acttcgggac gtcgttcaat gccctgttct ttcagcgtcc ggcagtcaat
      961 gaagcccctt tcaaagccat gctttctcat atgttcgttg tgcttcacct cccagcgttt
     1021 tcgcaattcc cgcaactgct ctttttgctg agtgggggtc aggtttccgg aatccttttt
     1081 cgcacctccc cggtcgggat acctggcgtt atagcgccgg aaatactgtt ccggtgtacg
     1141 ttcaatgcca tcattcacac gctgactcat catgatatgt gcatgaggct gttcgccgcc
     1201 gtcaatgctc gctttcggat tgtgaatggc aaaactccag gcgtgccgtt cgccagcttc
     1261 ctgacgtaca aaatcctgca ccagttcaag gcgctgttcc ggtgtcagtt cacggggtaa
     1321 tgcaatttca agttccctat aggtcgaacc gttcgcacgt tcaaactggt cagccgcctg
     1381 ccagaaatgg gaaggattat ccctggccca ttccggcatg tttccgtgtg cggaatattc
     1441 caaatcttga cggtcgcggt atttctcttt gcgctcgatg taatcggcat ggttggcagc
     1501 ctgtcccttg ccaccaaatt taacgctgag gtgataggtc gccacggttt cagccctccg
     1561 caggagcgca ctgtttctca acgaaggaga gaaacatata agtgcgcttc cttctaattt
     1621 ttcgaattag tacggaaggt aacccctgca cccaccgacc gcaagcgata tactgcacga
     1681 ccacaacaca ccgcaaaaaa acaccaggga gcaccaccaa catgcgcatc agcaacatcg
     1741 aatggctgaa gaaacggatc gagttcatca ggaagctcgg aaaacagacc gcacgacaga
     1801 gacagatcat tgacctgctc gacaacgagg acagactcac agaacaggaa cgcaaacttc
     1861 tccacgtact agcaacagcc gaaaaaaacg acttacaggc acaggagagc gaacgaaaac
     1921 aggcgatcca gaaacgaatc gaggggaaga aacaacgacg ggaacgcaat caccggctct
     1981 ttctcgcggc aggactactg atcgaagcag gactggtgga cacaaaaaca ggcgagctat
     2041 gctataaaaa agacatgtta ttacaaagac ttaaacccat taagtacgat ctagacacat
     2101 gcccaaaccc agacgcgtaa gtgttcccgt tcacgttccg gtgccctggt gcttcctcgc
     2161 tcattcgctc gctacgctcg gtcatgagac tgcggcgagc actaccacct caatcaagcg
     2221 aaaagccgca ccattgctgg gctgacaagc agaaaccacc caaaaaatag ctgctcaaaa
     2281 agccgccgga aaggcgctgt tatctgataa ggctatttgt gcattgccca attacggatc
     2341 gtattacgat gtacattcag catcgccgca atagctttct gagaatatcc attctccagc
     2401 atacataacg ccatgaaacg cttgtcctcg taggcttcgc ctttagcgat cccccccttg
     2461 cgaccgcgtg cggcctgtac agcggaaaac ccttctggcg agaattttct atgcgtgtat
     2521 ttcgcaatgc tcctgccaat agcccggcat tcagctggtg aaagcggaac aggaagcgat
     2581 gcgttgtaca tttcgacacg ctgaatcacg gcatcaagcc actgtgagaa ttcaggccag
     2641 ccctgacgaa tcgccctgta agcccatttg cgcgtcattt cgaacaggtg gcagtttcgc
     2701 cccatcccgt aatgtttatc gatgctacgg cgcgctgagg cgctcaaatc gagataatca
     2761 gccagttcat cgagggtata ggcttcctcg cgccactcca tcaccagcca ttccaggtgg
     2821 aacggatttt tgcaaatcag gccgctgtag ttcacatccg cacaaagttt ctcacacagc
     2881 gaacgttcaa ccgctgcggc atatttcagc gccttaaccg aggcatcagg cgcggttctc
     2941 acggcgatgt tgagtgcgta gagcaggtga gcgtgtccgt tcacaggatt tttaacggtg
     3001 atgttcggcg cgggagcgtt ccggtcgctc cagtcaatcg cagccccctc gcggtcaacg
     3061 tcaaaaacca gccagaactg tgcatgaggc tggttctgct ggatataccg tgcaagaagc
     3121 gcgcgccctt tgccggagat acgaaggccg aaagcgagat catccgtgtg atagggacgg
     3181 tggggtaaat tttcttcgaa gtattgaagc gcggcgctca tgtgttacga ttccttccag
     3241 gttatcgacc gacatcgccc ctgtattttc gtgaaagtct tggcggatag acacgaattt
     3301 caggggcgtt tgtttttcag tcgcttacac tacccccaac gacaaaacct gtcgagcctt
     3361 cccgcaaatc cgatccattt accataactc ccgttgtacg caccccataa aacgccctca
     3421 atcgctttct gacgcaaaaa acgcaaaaag acaaggaaac accaggctga accagaaaaa
     3481 tgcgtcagaa tcgttttcag ggcgttgcat aactatgcac gaaagcgatg tgcgatcggt
     3541 ttcgtgcgtt ctgaacaccg aacggctcaa aacgacaagt tttctcccct ggtgcgtggc
     3601 gctctgacgg ggtttcctgt ttccaaactt cacatctcgc caggacaaaa aaaaccgacc
     3661 tggggtcggt ggcaagaatc agagtctatg ggaaatattt tagttatcgg atgctcaata
     3721 ttcacaaacg caaaaaatgc ctcagtgatg ccctaaaaca acctttccag aaggagtccg
     3781 cgctttacga tgcacggaag agataaaaaa ccccacaaaa gtggggaacc aagagaaaca
     3841 acaattacag gttgttggtt gtgttcgaac aacgatgaag tagctacgct aaataataat
     3901 taccgtacag gccctacata ttcaacacta aatttaagat cctgacgaag gatttataaa
     3961 tgcacgaaaa cacatagaca gacaaattca ctaaattcat acacaaaaac aagtgtagtt
     4021 ttctaagtaa attagattcg acaaccaatt agtttatcca catcgcattc cactcttgct
     4081 acaccaatca catttcttga tatgtaatag cagaaaaaat aaaaccagac accaatagac
     4141 caaaaaacaa acattcaaca agaaacattt tgaacaatat cacaaaactg tcacaaccat
     4201 catcagacta ttgaaaaaaa ctggttgctg ttttataaaa gcgactgtaa caacctccac
     4261 tctatagttc cattagttta aagtgtcaca caaactgagc atccatttgt tttccttact
     4321 tttgcccctg tgttccaagg ggttttttta taacttaaag attagcacac cacacaaaaa
     4381 caataaaaaa caacaagtta tacaataaaa acagtactat ccatacagct aattacatat
     4441 aaataccatt aaaccatctg gacattaaaa ataaactctt ataacataaa attaatgcgg
     4501 ttccggtacc atatgtcagg gataatgtgc aaatggaggt atcggaactg catttcagtt
     4561 cattattaat taatgacaaa aaaaaccctc tgagagcgca gagggggtaa ggaatgttcc
     4621 cggtcataat atgtcgatag tgtttatata catacagaac aagaacaaca accaaccaac
     4681 taataaatat tttatttttt agagctgaaa ccttaaagct cacttccatt ccatacattg
     4741 ttataaacct tatgttgaaa actgaaagta agtacggcag acttattaag ttgcgtgcag
     4801 tcttttcacg ttatacaaac aagaacacct gcgaagttat ttctccttac atatccccct
     4861 atgccaacgg gggatttttt ctggtttact aacgcataaa taatagtgac cccctatcat
     4921 tcacgaaccg tgataatata aggattatca tcatggcaaa attaggtgaa aacaccccac
     4981 atattatcga tgctactgta gattttatgg catcaagcca ggcatttcgt gagtatctga
     5041 gaaaacttcc ccctgatcac acaataccgc cagatcttac tgatgagcac gtaccgttat
     5101 atatacgaag gctggaatac tatcgactgc tttatcgacc aaagc
//
