LOCUS       CP099251                2676 bp    DNA     circular BCT 13-APR-2023
DEFINITION  Escherichia coli strain RHB11-E4-C01 plasmid unnamed2, complete
            sequence.
ACCESSION   CP099251
VERSION     CP099251.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15488533
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB11-E4-C01
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 147x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/15/2022 20:10:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,020
            CDSs (total)                      :: 4,895
            Genes (coding)                    :: 4,689
            CDSs (with protein)               :: 4,689
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 206
            CDSs (without protein)            :: 206
            Pseudo Genes (ambiguous residues) :: 0 of 206
            Pseudo Genes (frameshifted)       :: 76 of 206
            Pseudo Genes (incomplete)         :: 134 of 206
            Pseudo Genes (internal stop)      :: 46 of 206
            Pseudo Genes (multiple problems)  :: 44 of 206
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB11-E4-C01"
                     /isolation_source="Pooled sediment sample collected from
                     floor of cattle farm"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(2359..2676,1..6))
                     /locus_tag="NFJ69_25085"
     CDS             complement(join(2359..2676,1..6))
                     /locus_tag="NFJ69_25085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="WFW34691.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="nikA protein"
     gene            290..484
                     /locus_tag="NFJ69_25090"
     CDS             290..484
                     /locus_tag="NFJ69_25090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WFW34692.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALNADEQAAMCEKLHELA
                     EELQNSIQTRFEAENLTGI"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1327..1731)
                     /locus_tag="NFJ69_25095"
     CDS             complement(1327..1731)
                     /locus_tag="NFJ69_25095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WFW34693.1"
                     /translation="MSGGNNFKMSQDYEIAQPKKKMAYPILVEEWDFLIGKIESISDDD
                     NIYHTIGSIFLGVAGSALIAALTLDLPSDDGYMPRQILISWYVFWASLICGVLSMFFGR
                     KNKKINRTSAKDIVQQMRLIEKRYEQGSMD"
                     /name="hypothetical protein"
     gene            complement(1728..2000)
                     /locus_tag="NFJ69_25100"
     CDS             complement(1728..2000)
                     /locus_tag="NFJ69_25100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WFW34690.1"
                     /translation="MSDVKSDQIIKYLSEKWGGSKCPLCKKGRWNVSDKIFELREFHEG
                     NVVIGAGPIVPIIPVTCDNCGNVVLVNAIISGCLSADNKQDGGSK"
                     /name="hypothetical protein"
     Typing          complement(16..252)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.58"
                     /coverage="100.0"
                     /evalue="1.62e-119"
     Typing          519..1022
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.312"
                     /coverage="95.0"
                     /evalue="3.85e-154"
     Typing          complement(2363..2676)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.095"
                     /coverage="98.0"
                     /evalue="6.549999999999999e-61"
ORIGIN
        1 tgtcataaaa catggctccg tatctgacat gggtgtcggg gcaaagccct gaccaaggca
       61 attgtaatag cgtgcatgta tgcgcggtat aacaattgca catcctgtcc tgtcagcaag
      121 ctcgaattac cgacaaaggc acggcagttt tttggcagtg acagtgttgt ctgacagaaa
      181 tgcccgtcaa aaagccaagc ggcagaatag cggcagcaaa acagtcgaaa agtggtacgc
      241 gatataatca cacaacacag atgacagaca gaacggagaa aacgaaaaga tgaacaagca
      301 gcagcaagcc gtactcaaca tggcgggatt cataaaaagt cagagcctga cgctgctcga
      361 aaaactggac gcactcaatg ccgacgaaca ggccgccatg tgtgagaaac tgcacgaact
      421 cgcagaagaa ctccagaaca gcatacagac acgctttgaa gcggaaaatc ttaccgggat
      481 ataacgctcc agacaggatg cagaacggca tagaggagcg taaggagaaa atgtcacata
      541 aggcgctctt ccgcttcctc gctcactgac tcgctacgct cggtcgttcg actgcggcga
      601 gcggtgtcag ctcactcaaa ggcggtaata cggttatcca cagaatcggg ggataaaacc
      661 ggaaagaaca tgtgagcaaa agacaaagac ccagaagaag ggcgcgcggg aggcgttttt
      721 ccataggctc cgcccccctg acgagcatca taaaaaccga cgctcaagtc agaggtggcg
      781 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
      841 tcctgttcct gcctttcggt ttaccaatgt cattccgcag ttatggccgc gtttatctca
      901 ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcacg
      961 aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgactt gaatccaaac
     1021 cgagaagaca cgacttatcg ccactggcag cagccactgg taaccgaatt agaggagtta
     1081 gccttgaagt catgcgcctg ttaaggctaa actgaaagaa caagttttgg tgactgcgct
     1141 cctccaagcc agttacctcg gctcaaagag ttggtagctc tgagaacctt cgaaaaacca
     1201 ccttgccggg tggttttttc gtttcataga caagagacta cgacgacaag acaaggatct
     1261 caagaagatc cttacattat gccccccctt ggcacgccgt aacctcgcta aacgttcagc
     1321 atccagttaa tccatactac cttgctcgta acgtttctct atcaatctca tttgttgcac
     1381 aatatccttc gcagaagttc tattaatctt tttattcttt ctaccaaaaa acatagaaag
     1441 cactccacaa ataaggcttg cccaaaatac gtaccatgaa attaatatct gccttggcat
     1501 ataaccatca tctgatggca aatctaaagt taatgctgct atcaatgccg agccagccac
     1561 acctaaaaat atagaaccaa tcgtatgata tatattatca tcatcactaa tactttctat
     1621 tttccctatt aaaaaatccc attcttcaac aagaataggg taagccattt tcttttttgg
     1681 ttgagcaatt tcatagtcct gagacatttt aaaattatta ccaccactca ctttgagcct
     1741 ccatcttgct tattgtcagc agataagcac cctgagataa tggcattaac aagaacaaca
     1801 ttcccgcaat tatcacaagt tacaggaata attgggacta tcggacctgc cccaataaca
     1861 acattacctt catgaaattc tcttagttca aaaattttgt cagaaacatt ccaacgccct
     1921 tttttacaca agggacattt gctccctccc cacttttcag acaaatattt aattatttga
     1981 tctgacttaa catcactcat attccctcca agcattcctt atactcatat atttattagc
     2041 gaacaatcac atcactcaat aatttattga tcaagtaact ttatagagta catccatatc
     2101 tgttttgtgc gcaaaaaaac ataaatataa aatcttacat ttaccataat accagtattg
     2161 tacgcaccac caaaacgagc tgaacgcgat tctgacgcaa ataccgcaaa aagagagatt
     2221 ttaccccgga aaaaccggaa aaatgcgtca gaatcatttt cagctcgctg catagctatg
     2281 catgaaagcg aatggtgatc actttggggg cttacggtgt tcataccgtc tgttttcgac
     2341 agttttctcc ccgggaagct aatctgccat aagcctggat aacagggcac ggtgatactc
     2401 cgtaatagcg atcagcacct ccgcatactc cctgtccccg atacgcttgc cgtcgataaa
     2461 gagttttttc tgtaacgccc ccagtctcat aacttctttc agtacccgat catcagtcag
     2521 tgagttaact ttcttaccga gagctgccgc ccgtaaaaaa ccggaaacgg tcttgccgca
     2581 ttcagcagcc ctttttctga tttcctggtc ttcttctgcc gtcagacgaa cagggcgact
     2641 gatagcccgc ctgcgcgtat tacttccact tcgttt
//
