LOCUS       CP099242                6230 bp    DNA     circular BCT 13-APR-2023
DEFINITION  Escherichia coli strain RHB13-SO-C01 plasmid unnamed5, complete
            sequence.
ACCESSION   CP099242
VERSION     CP099242.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15488537
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6230)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB13-SO-C01
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 105x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/15/2022 20:13:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,058
            CDSs (total)                      :: 4,936
            Genes (coding)                    :: 4,773
            CDSs (with protein)               :: 4,773
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 163
            CDSs (without protein)            :: 163
            Pseudo Genes (ambiguous residues) :: 0 of 163
            Pseudo Genes (frameshifted)       :: 69 of 163
            Pseudo Genes (incomplete)         :: 97 of 163
            Pseudo Genes (internal stop)      :: 35 of 163
            Pseudo Genes (multiple problems)  :: 34 of 163
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB13-SO-C01"
                     /isolation_source="Pooled soil sample collected from floor
                     of pig farm"
                     /db_xref="taxon:562"
                     /plasmid="unnamed5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(5887..6230,1..190)
                     /locus_tag="NFJ73_25260"
     CDS             join(5887..6230,1..190)
                     /locus_tag="NFJ73_25260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WFW48839.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            411..2138
                     /locus_tag="NFJ73_25265"
     CDS             411..2138
                     /locus_tag="NFJ73_25265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin"
                     /protein_id="WFW48840.1"
                     /translation="MSGGDGRDHNSGAHNTGGNINGGPTGLGGNGGASDGSGWSSENNP
                     WGGGSGSGVHWGGGSGNGNGGGNGNSGGGSNSSVAAVAFGFPALATPGAGTLGIAVSGE
                     ALSAAIADIFAALKGPFKFSAWGIALYGILPSEIAKDDPNMMSKIVTSLPAETVTNVQV
                     STLPLDQATVSVTKRVTDVVKDTRQHIAVVAGVPMSVPVVNAKPTRTPGVFHASFPGVP
                     SLTVSTVKGLPVSTTLPRGITEDKGRTAVPAGFTFGGGSHEAVIRFPKESGQKPIYVSV
                     TDVLTPAQVKQRQDEENRLQQEWNDTHPVEVAERNYEQARAELDQANKDVARNQERQAK
                     AVQVYNSRKSELDAANKTLADAKAEIKQFERFAREPMAAGHRMWQMAGLKAQRAQTDVN
                     NKKAAFDAAAKDKSDADAALGAALERRKQKENKEKDSKDKLDKESKRNKPGKATGKGKP
                     VGDKWLDDAGKDSGAPIPDRIADKLRDKEFKSFDDFRKKFWEEVSKDPELSKQFKDSNK
                     TNIQKGKAPFARKKDQVGGRERFELHHDKPISQDGGVYDMDNIRVTTPKRHIDIHRGK"
                     /name="colicin"
     gene            2141..2401
                     /locus_tag="NFJ73_25270"
     CDS             2141..2401
                     /locus_tag="NFJ73_25270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WFW48841.1"
                     /translation="MELKHSISDYTEAEFLEFVKKICRAEGATEEDDNKLVREFERLTE
                     HPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKPGFKQG"
                     /name="bacteriocin immunity protein"
     gene            2463..2606
                     /locus_tag="NFJ73_25275"
     CDS             2463..2606
                     /locus_tag="NFJ73_25275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0019867 - outer membrane [Evidence IEA]; GO_process:
                     GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WFW48842.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     gene            3103..4122
                     /locus_tag="NFJ73_25280"
     CDS             3103..4122
                     /locus_tag="NFJ73_25280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WFW48843.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREDAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSENQSDKGKKS
                     AAVRWTANDDKRRRALDMYILGASTEDIAVAVGVSSRTIRRWMDNSGEWLTKKQIIKC"
                     /name="replication initiation protein"
     gene            complement(4383..4781)
                     /locus_tag="NFJ73_25285"
     CDS             complement(4383..4781)
                     /locus_tag="NFJ73_25285"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WFW48837.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQENERKQAVQNRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQELKEIKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            4958..5884
                     /locus_tag="NFJ73_25290"
     CDS             4958..5884
                     /locus_tag="NFJ73_25290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WFW48838.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIEGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     PTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFETAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREYGFD
                     MRDGVSRQERTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     BGC             1..6230
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          3104..3878
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.778"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          4959..5881
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.403"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agggtatagc tggaaatggc tgggtggggt gtcagtggct tttctggtta tgttctgtgt
       61 gctcggatgg ggaatgaaaa caatactcga caggaactac aacgccacag cggcggtgta
      121 tcagaaagtt gtgacactgg aagataaact ggtggagaaa cctgtcacgg gtaagagccg
      181 gaaaaactga cgcgcttcgc ttgtccaacg cctcacgacc cgcgaaagga tttcgcgttc
      241 cgttcaccgt tttcatgagt gattcctccg aactgccaaa aaatatcccc ccctgacttg
      301 acagaaaaaa taatgacgag tactttttga tctgtacata aaaccagtgg ttttatgtac
      361 agtattaact atgtaatcaa ttgttttaac gcttaaaaga gggaattttt atgagcggtg
      421 gagatggacg cgaccacaac agtggcgcac ataacacagg tggtaacatt aatggcggcc
      481 ctacggggct tggtggaaat ggtggggctt ctgacggttc aggatggagt tcggaaaata
      541 atccatgggg tggcggttcc ggtagtggtg ttcactgggg aggtggctcc ggcaatggta
      601 atggcggggg gaatggtaat tccggtggtg gcagcaattc atccgtagca gcagtggcat
      661 ttggttttcc tgctttggca actcctggtg ccggaacgct gggtatcgcc gtctctggtg
      721 aggctttatc tgcggcaata gctgatatct ttgcagccct gaaaggtccg tttaaattca
      781 gcgcatgggg tattgcgctt tacggcattc tgccatctga aatagcaaaa gatgacccga
      841 atatgatgtc aaagattgtg acgtcattac cggcagaaac agtgacgaat gttcaggtca
      901 gtacacttcc gctggaccag gcaactgtca gcgtgacgaa acgtgtgaca gatgttgtga
      961 aggacacacg acagcatatt gccgtagttg caggtgtgcc tatgagtgtg cctgttgtga
     1021 atgccaaacc aacacgtact ccaggtgtat ttcacgcatc atttcctggt gtcccttctc
     1081 tgacagtcag cactgtcaaa ggccttccgg tgtcaacaac ccttccccgt ggtattacgg
     1141 aggataaagg ccggactgcc gttcctgcag gatttacctt tggtggtggt tcacatgaag
     1201 cggtgatccg ttttccgaaa gaaagtgggc agaagccgat ttatgtatca gtgacagatg
     1261 ttcttacccc tgcacaggta aaacaacgtc aggatgagga aaatcgcctc cagcaggaat
     1321 ggaatgacac acatcctgta gaagtggctg aacgcaatta tgaacaggcc cgcgcagaac
     1381 tggatcaggc taataaagat gttgccagaa accaggaacg acaggctaaa gctgttcagg
     1441 tttataattc tcgtaaaagt gaacttgatg ctgcgaataa aactcttgct gatgcaaagg
     1501 ctgaaataaa acaattcgag cgatttgccc gagaaccaat ggctgctggt caccgaatgt
     1561 ggcaaatggc agggcttaag gcccagcggg cacagacgga tgtaaataat aagaaggctg
     1621 catttgatgc tgctgcaaaa gataagtctg atgctgatgc tgcgttaggt gctgcgctgg
     1681 aacgccgtaa gcagaaggaa aataaagaaa aggactctaa ggataaatta gataaggaga
     1741 gtaaacggaa taagccaggg aaggcgacgg gtaaaggaaa accagttggt gataaatggc
     1801 ttgatgatgc gggtaaagat tcaggagcgc caattcctga tcgcattgct gataagttgc
     1861 gtgataaaga atttaaaagt tttgacgatt tccggaagaa attctgggaa gaagtgtcaa
     1921 aagatcccga gcttagtaag caatttaaag acagtaataa gacgaacatt caaaagggaa
     1981 aagcaccttt tgcaaggaag aaagaccaag taggtggtag ggaacgcttt gaattacatc
     2041 atgataaacc aatcagtcag gatggtggtg tctatgatat ggataatatc agagtgacca
     2101 cacctaagcg acatattgat attcatagag gtaagtaaaa atggaactga aacatagtat
     2161 tagtgattat accgaggctg aatttctgga gtttgtaaaa aaaatatgta gagctgaagg
     2221 tgctactgaa gaggatgaca ataaattagt gagagagttt gagcgattaa ctgagcaccc
     2281 agatggttca gatctgattt attatcctcg cgatgacagg gaagatagtc ctgaagggat
     2341 tgttaaagaa attaaagaat ggcgagctgc taacggtaag ccaggattta aacagggcta
     2401 aaatatgaat gccggttgtt tatggatgaa tggctggcat tctttcacaa caaggagtcg
     2461 ttatgaaaaa aataacaggg attattttat tgcttcttgc agtcattatt ctggctgcat
     2521 gtcaggcaaa ctatatccgg gatgttcagg gcgggactgt atccccgtca tcaacagctg
     2581 aagtgaccgg attagcaacg cagtaacccg aaatcctctt tgacaaaaac aaagcgtgtc
     2641 aggctgattc tgatgcgctt ttttttgaaa tgtcacaaaa attccatgcg ggagatgaga
     2701 gctaaaatcc ccgtgcagaa ctttccaccc agggcgagaa aacttgtcat tttgacctgt
     2761 tcgcccttcg gaacagtcga aatcgatcgc gcatcgcttt cgtgcatagt tatgcagccc
     2821 tctaaaaacg attctggcgc gtttttctgg tttggcctgg tgttttcttg tctttttgcg
     2881 ttttttgcgt cagaaagcga ctgagggcgt tttaaggggt gcgtacaacg ggagttatgg
     2941 taaatggatc ggtttttcgg gaaggatcga caggatttgc cgttgggtgt agtgtaagcg
     3001 gctgaaaaac aaacgcccct gaaatcatgt ccagatccgg caagattcaa catgaaatac
     3061 agagggcgtg taggtcgcat aacccggaga gaatcgtaac acatgagcgc cgcgcttcaa
     3121 tacttcgacg aaaatttacc ccatcgtccc tatcacacgg atgatctcgc tttcggcctt
     3181 cgcatctccg gcaaagggcg tgcgcttctt gcgcggtaca tccagcagaa ccagcctcat
     3241 gcgcagttct ggctggtttt tgacgttgac cgcgaggggg ctgcgattga ctggagcgac
     3301 cggaacgccc ccgcgccgaa catcaccgtt aaaaatcctg tgaacggaca cgctcacctg
     3361 ctctacgcac tcaacatcgc cgtgagaacc gcgcctgatg cctcggttaa ggcgctgaaa
     3421 tatgccgcag cggttgaacg ttcgctgtgt gagaaacttt gtgctgatgt gaactacagc
     3481 ggcctgattt gcaaaaatcc gttccacctg gaatggcagg tgatggagtg gcgcgaggac
     3541 gcctataccc tcgatgaact ggcagattat ctcgatttga gcgcctcaga gcgtcgtagc
     3601 atcgataaac attacgggat ggggcgaaac tgccacctgt tcgaaatgac gcgcaaatgg
     3661 gcttacaggg cgattcgtca gggctggcca gcgttctcac agtggcttga agcggtgatc
     3721 cagcgtgtcg aaatgtacaa tgcatcgctt cccgttccgc tttcactggc tgaatgtcgg
     3781 gctattggca agagcattgc gaaatacacg cacaggaact tcacgccgga aactttcgca
     3841 cagtatgtgg ctgatacgca cacgccagaa attcaggcag cacgcggtcg caagggcggg
     3901 aaggccaatt cgtcagaaaa tcagtcggac aaagggaaaa aaagcgccgc tgttcggtgg
     3961 actgctaacg acgacaaacg tcgtagggcg cttgatatgt atatcttggg agcgtcaacg
     4021 gaagatattg cagtggcggt tggtgttagc agccggacta ttcgacgctg gatggataat
     4081 tctggagaat ggctaaccaa gaaacaaata attaaatgtt agttaacaaa gaagcaacac
     4141 taccccccgt tttttgttaa tgggatgtta aattcgcgga catgaacaag cctatatcag
     4201 ataacagcgc ctttcggcgt ctttttgagc agcgggcttt ccggcggttt tctcctctca
     4261 gcccagcaat ggtgcggctt accgcatgat tgaggtggtc gtgctcgccg cagtctcatg
     4321 accgagcgta gcgagcgaat gagcgaggaa gcaccaggga accggaacac ggatggaggc
     4381 acttacgcgt ctgggtttgg ggatgtctcc agatcgtact taatttcctt aagttcctgt
     4441 aatatcctgt cttttttata gcatagctcg cctgtttttg tgtctaccag tcctgcttcg
     4501 atcagtagcc cagccgcgag aaagagtcgg tgattgcgtt ctcgtcgttg tttttttccc
     4561 tcgattcgat tttggaccgc ctgttttcgt tcgttctcct gtgcctgtaa gtcgtttttt
     4621 tccgctgttg ccagtacgtg gaggagtttg cgttcctgtt ctgtgagtcc tgcttcgttg
     4681 tcgagcaggt caataatctg tctctgtcgt gcggtctgtt ctccgagctt cctgatgaat
     4741 ccgatccgtt tcttcagcca ttcgatgttg ctgatgcgca tgttcgtggt gctccctggt
     4801 gtttttttgc ggtgtgttgt ggtcgtgcag tatatcgctt gcggtcggcg ggtgcaggag
     4861 gtatcttccg tgctaattcg taaaattaga aggaagcgca cttatatgtt tctctccttc
     4921 gtcgagaaac agtgcgctcc tgcggagggc tgaaaccgtg gcaacctatc acctcagcgt
     4981 taaatttggt ggcaaggggc aggctgcaaa ccacgccgat tacatcgagc gcaaagagaa
     5041 ataccgcgac cgtcaggatt tggaatattc cgcacacgga aacatgccgg aatgggcgcg
     5101 agataatccc tcacatttct ggcaggcggc tgaccagttt gaacgtgcaa acggttcgac
     5161 ttatcgggaa ctggagatag cgctgccccg tgaactgaca ccggaacagc gccttgaact
     5221 ggtgcaggat tttgtacgtc aggaagctgg cgaacggcat gcatggagtt ttgccattca
     5281 caatccgaaa gcgagcattg agggaggcga acagcctcat gcgcatatca tgatgagtca
     5341 gcgtgtgaat gatggcattg aacgtacacc ggaacagtat ttccggcgct ataacgccag
     5401 gtatcccgag cggggaggtg cgaaaaagga ttcaggaagc ctgactccca ctcagcaaaa
     5461 agagcagttg cgggaattgc gaaaacgctg ggaggtgaag cacaacgaac atatgagaaa
     5521 gcatggcttt gaaaggggct tcattgactg ccggacgctg aaagaacagg gcattgaacg
     5581 acgtcctgaa gtgcatctgg gttttgaaac tgccggacgg cttgatgatg cccagcgtca
     5641 tgacatcatg caaaaacggt ctgaaccgga ctattcccgc catcttgaac aggcgcgaac
     5701 ggtcgggcat gacatcaggg aatggctgga cactccggtt aagcaacatg aactggtcag
     5761 aaggctgcaa catgatgttg cgcgggagta tggctttgac atgagagacg gagtgagcag
     5821 acaggagcga actgaacctg aacgggttaa atcaccgtca cgtggatttt ctatgggatt
     5881 ctgaacatgg ccagcacaga agaaaaatta ctcacgctcc tgttatcggt ggagcatctg
     5941 caacataccg caatggagca acagaaagct gttgctgatt ctctggctgt ggcaagggaa
     6001 acacttcgcg gatatcggga tatttctgac ggtgtggcaa aacaggtcag ggatgaagtc
     6061 agaaatgagg tgcagaagct ggacgtggca ggactgatag gtgagcgcat cagccaggat
     6121 tttcagcggc tggaagagtc agtcgcaaac atgaaggcaa acgcggatgc cctgaacagg
     6181 gatattgcga caattcacag caatatcgtg caggaataca acacccttaa
//
