LOCUS       CP099241                6668 bp    DNA     circular BCT 13-APR-2023
DEFINITION  Escherichia coli strain RHB13-SO-C01 plasmid unnamed4, complete
            sequence.
ACCESSION   CP099241
VERSION     CP099241.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15488537
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6668)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6668)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB13-SO-C01
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 105x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/15/2022 20:13:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,058
            CDSs (total)                      :: 4,936
            Genes (coding)                    :: 4,773
            CDSs (with protein)               :: 4,773
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 163
            CDSs (without protein)            :: 163
            Pseudo Genes (ambiguous residues) :: 0 of 163
            Pseudo Genes (frameshifted)       :: 69 of 163
            Pseudo Genes (incomplete)         :: 97 of 163
            Pseudo Genes (internal stop)      :: 35 of 163
            Pseudo Genes (multiple problems)  :: 34 of 163
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB13-SO-C01"
                     /isolation_source="Pooled soil sample collected from floor
                     of pig farm"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(6291..6668,1..1191))
                     /gene="cea"
                     /locus_tag="NFJ73_25210"
     CDS             complement(join(6291..6668,1..1191))
                     /gene="cea"
                     /locus_tag="NFJ73_25210"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WFW48831.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            1451..1888
                     /locus_tag="NFJ73_25215"
     CDS             1451..1888
                     /locus_tag="NFJ73_25215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WFW48832.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="entry exclusion protein 1"
     gene            1969..2388
                     /locus_tag="NFJ73_25220"
     CDS             1969..2388
                     /locus_tag="NFJ73_25220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WFW48833.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(2706..2960)
                     /locus_tag="NFJ73_25225"
     CDS             complement(2706..2960)
                     /locus_tag="NFJ73_25225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WFW48834.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2967..3485)
                     /locus_tag="NFJ73_25230"
     CDS             complement(2967..3485)
                     /locus_tag="NFJ73_25230"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WFW48835.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(3523..3954)
                     /locus_tag="NFJ73_25235"
                     /pseudo=""
     CDS             complement(3523..3954)
                     /locus_tag="NFJ73_25235"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4163..4486)
                     /locus_tag="NFJ73_25240"
     CDS             complement(4163..4486)
                     /locus_tag="NFJ73_25240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WFW48828.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4550..4741
                     /locus_tag="NFJ73_25245"
     CDS             4550..4741
                     /locus_tag="NFJ73_25245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WFW48829.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5768..5905)
                     /locus_tag="NFJ73_25250"
     CDS             complement(5768..5905)
                     /locus_tag="NFJ73_25250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0019867 - outer membrane [Evidence IEA]; GO_process:
                     GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WFW48830.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            6067..6294
                     /locus_tag="NFJ73_25255"
     CDS             6067..6294
                     /locus_tag="NFJ73_25255"
                     /inference="COORDINATES: protein motif:HMM:NF015489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WFW48836.1"
                     /translation="MLYAIVISTILCPYSKYAIEHIAFNFIKKDFFERRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     Typing          complement(4827..4914)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          4925..5483
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.365"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2603..4173)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.809"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cttgtatttt tcaaaagcag cgagagcttc attcacattg ccgattttct tacctttaga
       61 cttatcagca agttcctgtg ccattttcga atatttttca ccatattttt cagtcagcgt
      121 ttgataaaag ctaactgttg catcaacagc atccttaatc tgtgaattaa ggagattatt
      181 ctgtgctttt ttcaaatttt cttcagcttc atgaacacga gcgataccgg cattacgatt
      241 attactgacc tgagaaatag ccttctggat ctgagttata tcagcattta tccggttaat
      301 acgtgtttct gatgctgtta cctgtttttg tttttcttct ctaatcttac cggccccaac
      361 ccgtcgtctg gttgcttcaa aaaaaggacg gttctgaagc ggatcattgg ctcttggtga
      421 tagttttttg accagctcat ccagttcttt atatttagcg gatgcctgag ccagttcatt
      481 tcgttttcca gcgagcgttt tcatttctgc atcacgggca tggatactgg agcttaaacg
      541 agaattgaga gtcttaatct ctccatccat tttcaccact tcagattgtg cagcagaaag
      601 ttttttttgg gcgatctcaa cagctttagc ttcttcactc aatgcagcca gtcgtttctc
      661 ttcagcttca gccagtttca actggcgttc tgtttcagcc ttctcccgtt caatctcttt
      721 acgtcgttgt tctgcttcct gaaaagcctt ttctgctgct tccgcttctt tacgggcttt
      781 ttcttctgct ttcgcaaggc gcaaacgctc tgcttccgcc tgcatagctg cattattagc
      841 atgagcaagc tctgttgctg aaggcgtacg tgaggcattg tgacgaagag cctcattcac
      901 gatatccttc aggcgctgag tcagcgcatc cctgtttgcc tttgctttcg cctgtgcttc
      961 cgctgcagct tttgcccggg cagcctgctc tgcctgtgtt ttctttaatt gagcagtaga
     1021 ccatttagca gttgcatgaa tagctgcaga actttcactt ttactgcctc cttttccacc
     1081 tccgccgcca gagccactcc cgtcaggagt accattcaaa agagtaataa ttacctgtcc
     1141 cttatcatca taaggaacac catctttata gtacgctacc gcggtttcca ttataaaatc
     1201 ctctttgact tttaaaacaa taagttaaaa ataaatactg tacatataac cactggtttt
     1261 atatacagca taaaagctac gccgctgcat tttccctgtc aagactgtgg acttccattt
     1321 ttgtgaaaac gatcaaaaaa acagtctttc acaccacgcg ctattctcgc ccgatgccac
     1381 aaaaaccagc acaaacatta ccgttctcag acctcattat gttttactga aactatgaga
     1441 tgagacatct atgggacact gtcactttat ggcatggcac acactccggg acgcactaaa
     1501 aatgacaggc agatcgcgtt cacagtttta ccgtgatatg cgcgcaggcc ttgtcagtta
     1561 ccgtaccggc agggacggac gacgggagtt tgaaaccagt gaactgatcc gggcatacgg
     1621 cgaattaaag cagaatgaga caccagaaag gcacagtgag ggacatgcag aaaatccaca
     1681 tgatcagcag acagaacgca ttctccggga gctgaatgag ctgaaacaat gcctgacgct
     1741 gatgcttgag gataaacagg cacaggatat ggatcgcaga cgccaggaag cagaacggga
     1801 acagctacaa aatgagatag cccagctcag gcaggcactg gaactggaaa agaaacgggg
     1861 attctggtcc aggttgttcg gtcgctgaac gctgtcagag actgatgata aaatagtctt
     1921 cggataataa ctcaccgaga ataaatactt taaggtaggg agacactcat gagacgtacc
     1981 ggaaacaaac tttgtcttat cgccatgata acagcaacag tagctctcac agcctgtacc
     2041 ccaaagggca gcgtggaaca acatacccgg cattacgtat atgcttctga tgacggtttt
     2101 gatcccaact tttccaccca aaaagccgac acaacacgaa tgatggtgcc tttttttcgg
     2161 cagttctggg atatgggagc taaagataaa gcgacaggaa aatcacggag tgatgtgcaa
     2221 caacgcattc agcagtttca cagccaggaa tttttaaact cactccgggg cacaactcaa
     2281 tttgcgggta ctgattaccg cagcaaagac cttaccccga aaaaatccag gctgctggct
     2341 gacacgattt ctgcggttta tctcgatggc tacgagggca gacagtaagt ggatttacca
     2401 taatccctta attgtacgca ccgctaaaac gcgttcagcg cgatcacggc agcagacagg
     2461 taaaaatggc aacaaaccac ccgaaaaact gccgcgatcg cgcctgataa attttaaccg
     2521 tatgaatacc tatgcaacca gagggtacag gccacattac ccccacttaa tccactgaag
     2581 ctgccatttt tcatggtttc accatcccag cgaagggcca tccagcgtgc gttcctgtat
     2641 ttccggctga cgctcccgtt ccagggataa cacatgttcg cgctcctgta tcagccgttc
     2701 ctctcttatc tccagttctc gctgtataac tggctcaagc gttctgtctg ctcgctcaag
     2761 cgttctgtct gctcgctcaa gtgctgcacc tgctgactca actgcatgac ccgctcgttc
     2821 agcatcgcgt tgtcccgttg cgtaagcgaa aacatcttct gcaattccac gaaggcgctc
     2881 tcccattcgc tcagccgctg catatagtcc tgttgcagct gctctaaggc gttcagcaaa
     2941 tgtgtttcca gctctgtcac tctgtgtcac tccttcagat gtacccactc tttcccctga
     3001 aagggaatca cctccgctga tttcccgtac ggaaggacaa ggaatttcct gttcccgtcc
     3061 tgcacaaact ccacgcccca tgtcttcgcg ttcagtttct gcaatgtctc ttcctgcttc
     3121 ctgatttctt ccaggttcgc ctgtatcctc cctccaagat accagagcgt cccgccactc
     3181 gcggtaaaca ggagaaagac tatccccagt aacatcatgc ccgtattccc tgccagcttt
     3241 aacacgtccc tcctgtgctg catcatcgcc tctttcaccc cttcccggtg tttttccagc
     3301 gattcctctg tcgaggctgt gaacagggct atagcgtctc tgattttcgt ctcgtttgat
     3361 gtcacagcct cgcttacaga ttcgccgagc ctcctgaact cgttgttcag cattttctct
     3421 gtagattcgg ctctctcttt cagctttttc tcgaactccg cgcccgtctg caaaagattg
     3481 ctcataaaat gctcctttca gcctgatatt cttcccgccg ttcggatctg caatgctgat
     3541 actgcttcgc gtcaccctga ccacttccag ccccgcctca gtgagcgcct gaatcacatc
     3601 ctgacggcct tttatctctc cggcatggta aagtgcatct atacctcgcg tgacgccctc
     3661 agcaagcgcc tgtttcgttt caggcaggtt atcagggagt gtcagcgtcc tgcggttctc
     3721 cggggcgttc gggtcatgca gcccgtaatg gtgattcacc agcgtctgcc aggcatcaat
     3781 tctaggcctg tctgcgcggt cgtagtacgg ctggaggcgt tttccggtct gtagctccat
     3841 gttcggaatg acaaaattca gctcaagccg tcccttgtcc tggtgctcca cccacaggat
     3901 gctgtactga tttttttcga gaccgggcat cagtacacgc tcaaagctcg ccatcacttt
     3961 ttcacgtcct cccggcggca gctccttctc cgcgaacgac agaacaccgg acgtgtattt
     4021 cttcgcaaat ggcgtggcat cgatgagttc ccggacttct tccggattac cctgaagcac
     4081 cgttgcgcct tcgcggttac gctccctccc cagcaggtaa tcaaccggac cactgccacc
     4141 accttttccc ctggcatgaa atttaactat catcccgcgc cccctgttcc ctgacggcca
     4201 gacgcagccg gcgcagctca tccccgatgg ccatcagtgc ggccaccacc tgaacccggt
     4261 caccggaaga ccactgcccg ctgttcacct tacgggctgt ctgattcagg ttatttccga
     4321 tggcggccag ctgacgcagt aacggcggtg ccagtgtcgg cagttttccg gaacgggcaa
     4381 ccggctcccc caggcagacc cgccgcatcc ataccgccag ttgtttaccc tcacagcgtt
     4441 caagtaaccg ggcatgttca tcatcagtaa cccgtattgt gagcatcctc tcgcgtttca
     4501 tcggtatcat taccccatga acagaaatcc cccttacacg gaggcatcag tgactaaaca
     4561 ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc cagacattaa cgctgctgga
     4621 gaagctcaac gaactggacg cagatgaaca ggccgatatt tgtgaatcgc ttcacgacca
     4681 cgccgatgag ctttaccgca gctgcctcgc acgtttcggg gatgacggtg aaaacctctg
     4741 acacatgcag ctcccggaga cggtcacagc ttgtctgtga gcggatgccg ggagctgaca
     4801 agcccgtcag ggcgcgtcag caggttttag cgggtgtcgg ggcgcagccc tgacccagtc
     4861 acgtagcgat agcggagtgt atactggctt aaccatgcgg catcagtgcg gattgtatga
     4921 aaagtacgcc atgccgggtg tgaaatgccg cacagatgcg taaggagaaa atgcacgtcc
     4981 aggcgctttt ccgcttcctc gctcactgac tcgctacgct cggtcgttcg actgcggcga
     5041 gcggtactga ctcacacaaa aacggtaaca cagttatcca cagaatcagg ggataaggcc
     5101 ggaaagaaca tgtgagcaaa agaccagaaa caggaagaag gccgcgtagc aggcgttttt
     5161 ccataggctc cgcccccctg acgagcatca caaaaataga cgctcaagtc agaggtggcg
     5221 aaacccgaca ggactataaa gacaccaggc gtttccccct ggaagctccc tcgtgcgctc
     5281 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     5341 ggcgctttct catagctcac gctgttggta tctcagttcg gtgtaggtcg ttcgctccaa
     5401 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     5461 tcgtcttgag tccaacccgg taaggcacgc cttaacgcca ctggcagcag ccactggtaa
     5521 ccggattagc agagcgatga tggcacaaac ggtgctacag agttcttgaa gtagtggccc
     5581 gactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     5641 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgttgg tagcggtggt
     5701 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     5761 atcttttcta ctgaaccgcg atccccgtca gtttagaaga ggaggatggt gcgatggtcc
     5821 ctccctgaac atcacgtata tagttagcct gacatccaac aaggaggttt atcgcgaata
     5881 ttcccacaaa aaatcttttc ctcataactc gatccttata aaatgaaaag aatatatggc
     5941 gaggtttaat ttatgagctt aagatactac ataaaaaata ttttatttgg cctgtactgc
     6001 acacttatat atatatacct tataacaaaa aacagcgagg ggtattattt ccttgtgtca
     6061 gataagatgc tatatgcaat agtgataagc actattctat gtccatattc aaaatatgct
     6121 attgaacaca tagcttttaa cttcataaag aaagattttt tcgaaagaag aaaaaaccta
     6181 aataacgccc ccgtagcaaa attaaaccta tttatgctat ataatctact ttgtttggtc
     6241 ctagcaatcc catttggatt gctaggactt tttatatcaa taaagaataa ttaaatccct
     6301 aacacctcat ttatagtatt aagtttattc ttatcaatat aggagcatag aattcctgta
     6361 acaatagcaa taccccaaat acctaatgta gttccagcaa gcaagctaaa aagtaaagca
     6421 acaacataac tcacccctgc atctgcagct ttcttctcta atgtaagaaa tagtggcttc
     6481 cagtcacctg tatccttaat ttttaggata tcagatacca catcatatcc aaaagaaaca
     6541 tgccccgtaa tcttcaagta cttggcaaac tgatctaaat gtttagccca gtcatcatac
     6601 ttcaccgatg ccaacgcatt aaaaatagca tcacgatcgg ctttgctgaa tttcttattt
     6661 aaaacatc
//
