LOCUS       CP099230                6230 bp    DNA     circular BCT 13-APR-2023
DEFINITION  Escherichia coli strain RHB13-SO-C03 plasmid unnamed5, complete
            sequence.
ACCESSION   CP099230
VERSION     CP099230.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15488539
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6230)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB13-SO-C03
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/15/2022 20:29:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 5,053
            CDSs (total)                      :: 4,931
            Genes (coding)                    :: 4,768
            CDSs (with protein)               :: 4,768
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 163
            CDSs (without protein)            :: 163
            Pseudo Genes (ambiguous residues) :: 0 of 163
            Pseudo Genes (frameshifted)       :: 69 of 163
            Pseudo Genes (incomplete)         :: 97 of 163
            Pseudo Genes (internal stop)      :: 35 of 163
            Pseudo Genes (multiple problems)  :: 34 of 163
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB13-SO-C03"
                     /isolation_source="Pooled soil sample collected from floor
                     of pig farm"
                     /db_xref="taxon:562"
                     /plasmid="unnamed5"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(5797..6230,1..100))
                     /locus_tag="NFJ75_25235"
     CDS             complement(join(5797..6230,1..100))
                     /locus_tag="NFJ75_25235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WFW58379.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(103..1029)
                     /locus_tag="NFJ75_25240"
     CDS             complement(103..1029)
                     /locus_tag="NFJ75_25240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WFW58380.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIEGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     PTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFETAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREYGFD
                     MRDGVSRQERTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            1206..1604
                     /locus_tag="NFJ75_25245"
     CDS             1206..1604
                     /locus_tag="NFJ75_25245"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WFW58381.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQENERKQAVQNRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQELKEIKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            complement(1865..2884)
                     /locus_tag="NFJ75_25250"
     CDS             complement(1865..2884)
                     /locus_tag="NFJ75_25250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WFW58382.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREDAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSENQSDKGKKS
                     AAVRWTANDDKRRRALDMYILGASTEDIAVAVGVSSRTIRRWMDNSGEWLTKKQIIKC"
                     /name="replication initiation protein"
     gene            complement(3381..3524)
                     /locus_tag="NFJ75_25255"
     CDS             complement(3381..3524)
                     /locus_tag="NFJ75_25255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0019867 - outer membrane [Evidence IEA]; GO_process:
                     GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WFW58376.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     gene            complement(3586..3846)
                     /locus_tag="NFJ75_25260"
     CDS             complement(3586..3846)
                     /locus_tag="NFJ75_25260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WFW58377.1"
                     /translation="MELKHSISDYTEAEFLEFVKKICRAEGATEEDDNKLVREFERLTE
                     HPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKPGFKQG"
                     /name="bacteriocin immunity protein"
     gene            complement(3849..5576)
                     /locus_tag="NFJ75_25265"
     CDS             complement(3849..5576)
                     /locus_tag="NFJ75_25265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin"
                     /protein_id="WFW58378.1"
                     /translation="MSGGDGRDHNSGAHNTGGNINGGPTGLGGNGGASDGSGWSSENNP
                     WGGGSGSGVHWGGGSGNGNGGGNGNSGGGSNSSVAAVAFGFPALATPGAGTLGIAVSGE
                     ALSAAIADIFAALKGPFKFSAWGIALYGILPSEIAKDDPNMMSKIVTSLPAETVTNVQV
                     STLPLDQATVSVTKRVTDVVKDTRQHIAVVAGVPMSVPVVNAKPTRTPGVFHASFPGVP
                     SLTVSTVKGLPVSTTLPRGITEDKGRTAVPAGFTFGGGSHEAVIRFPKESGQKPIYVSV
                     TDVLTPAQVKQRQDEENRLQQEWNDTHPVEVAERNYEQARAELDQANKDVARNQERQAK
                     AVQVYNSRKSELDAANKTLADAKAEIKQFERFAREPMAAGHRMWQMAGLKAQRAQTDVN
                     NKKAAFDAAAKDKSDADAALGAALERRKQKENKEKDSKDKLDKESKRNKPGKATGKGKP
                     VGDKWLDDAGKDSGAPIPDRIADKLRDKEFKSFDDFRKKFWEEVSKDPELSKQFKDSNK
                     TNIQKGKAPFARKKDQVGGRERFELHHDKPISQDGGVYDMDNIRVTTPKRHIDIHRGK"
                     /name="colicin"
     BGC             1..6230
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(2110..2884)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.778"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          complement(107..1029)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.403"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccagagaatc agcaacagct ttctgttgct ccattgcggt atgttgcaga tgctccaccg
       61 ataacaggag cgtgagtaat ttttcttctg tgctggccat gttcagaatc ccatagaaaa
      121 tccacgtgac ggtgatttaa cccgttcagg ttcagttcgc tcctgtctgc tcactccgtc
      181 tctcatgtca aagccatact cccgcgcaac atcatgttgc agccttctga ccagttcatg
      241 ttgcttaacc ggagtgtcca gccattccct gatgtcatgc ccgaccgttc gcgcctgttc
      301 aagatggcgg gaatagtccg gttcagaccg tttttgcatg atgtcatgac gctgggcatc
      361 atcaagccgt ccggcagttt caaaacccag atgcacttca ggacgtcgtt caatgccctg
      421 ttctttcagc gtccggcagt caatgaagcc cctttcaaag ccatgctttc tcatatgttc
      481 gttgtgcttc acctcccagc gttttcgcaa ttcccgcaac tgctcttttt gctgagtggg
      541 agtcaggctt cctgaatcct ttttcgcacc tccccgctcg ggatacctgg cgttatagcg
      601 ccggaaatac tgttccggtg tacgttcaat gccatcattc acacgctgac tcatcatgat
      661 atgcgcatga ggctgttcgc ctccctcaat gctcgctttc ggattgtgaa tggcaaaact
      721 ccatgcatgc cgttcgccag cttcctgacg tacaaaatcc tgcaccagtt caaggcgctg
      781 ttccggtgtc agttcacggg gcagcgctat ctccagttcc cgataagtcg aaccgtttgc
      841 acgttcaaac tggtcagccg cctgccagaa atgtgaggga ttatctcgcg cccattccgg
      901 catgtttccg tgtgcggaat attccaaatc ctgacggtcg cggtatttct ctttgcgctc
      961 gatgtaatcg gcgtggtttg cagcctgccc cttgccacca aatttaacgc tgaggtgata
     1021 ggttgccacg gtttcagccc tccgcaggag cgcactgttt ctcgacgaag gagagaaaca
     1081 tataagtgcg cttccttcta attttacgaa ttagcacgga agatacctcc tgcacccgcc
     1141 gaccgcaagc gatatactgc acgaccacaa cacaccgcaa aaaaacacca gggagcacca
     1201 cgaacatgcg catcagcaac atcgaatggc tgaagaaacg gatcggattc atcaggaagc
     1261 tcggagaaca gaccgcacga cagagacaga ttattgacct gctcgacaac gaagcaggac
     1321 tcacagaaca ggaacgcaaa ctcctccacg tactggcaac agcggaaaaa aacgacttac
     1381 aggcacagga gaacgaacga aaacaggcgg tccaaaatcg aatcgaggga aaaaaacaac
     1441 gacgagaacg caatcaccga ctctttctcg cggctgggct actgatcgaa gcaggactgg
     1501 tagacacaaa aacaggcgag ctatgctata aaaaagacag gatattacag gaacttaagg
     1561 aaattaagta cgatctggag acatccccaa acccagacgc gtaagtgcct ccatccgtgt
     1621 tccggttccc tggtgcttcc tcgctcattc gctcgctacg ctcggtcatg agactgcggc
     1681 gagcacgacc acctcaatca tgcggtaagc cgcaccattg ctgggctgag aggagaaaac
     1741 cgccggaaag cccgctgctc aaaaagacgc cgaaaggcgc tgttatctga tataggcttg
     1801 ttcatgtccg cgaatttaac atcccattaa caaaaaacgg ggggtagtgt tgcttctttg
     1861 ttaactaaca tttaattatt tgtttcttgg ttagccattc tccagaatta tccatccagc
     1921 gtcgaatagt ccggctgcta acaccaaccg ccactgcaat atcttccgtt gacgctccca
     1981 agatatacat atcaagcgcc ctacgacgtt tgtcgtcgtt agcagtccac cgaacagcgg
     2041 cgcttttttt ccctttgtcc gactgatttt ctgacgaatt ggccttcccg cccttgcgac
     2101 cgcgtgctgc ctgaatttct ggcgtgtgcg tatcagccac atactgtgcg aaagtttccg
     2161 gcgtgaagtt cctgtgcgtg tatttcgcaa tgctcttgcc aatagcccga cattcagcca
     2221 gtgaaagcgg aacgggaagc gatgcattgt acatttcgac acgctggatc accgcttcaa
     2281 gccactgtga gaacgctggc cagccctgac gaatcgccct gtaagcccat ttgcgcgtca
     2341 tttcgaacag gtggcagttt cgccccatcc cgtaatgttt atcgatgcta cgacgctctg
     2401 aggcgctcaa atcgagataa tctgccagtt catcgagggt ataggcgtcc tcgcgccact
     2461 ccatcacctg ccattccagg tggaacggat ttttgcaaat caggccgctg tagttcacat
     2521 cagcacaaag tttctcacac agcgaacgtt caaccgctgc ggcatatttc agcgccttaa
     2581 ccgaggcatc aggcgcggtt ctcacggcga tgttgagtgc gtagagcagg tgagcgtgtc
     2641 cgttcacagg atttttaacg gtgatgttcg gcgcgggggc gttccggtcg ctccagtcaa
     2701 tcgcagcccc ctcgcggtca acgtcaaaaa ccagccagaa ctgcgcatga ggctggttct
     2761 gctggatgta ccgcgcaaga agcgcacgcc ctttgccgga gatgcgaagg ccgaaagcga
     2821 gatcatccgt gtgataggga cgatggggta aattttcgtc gaagtattga agcgcggcgc
     2881 tcatgtgtta cgattctctc cgggttatgc gacctacacg ccctctgtat ttcatgttga
     2941 atcttgccgg atctggacat gatttcaggg gcgtttgttt ttcagccgct tacactacac
     3001 ccaacggcaa atcctgtcga tccttcccga aaaaccgatc catttaccat aactcccgtt
     3061 gtacgcaccc cttaaaacgc cctcagtcgc tttctgacgc aaaaaacgca aaaagacaag
     3121 aaaacaccag gccaaaccag aaaaacgcgc cagaatcgtt tttagagggc tgcataacta
     3181 tgcacgaaag cgatgcgcga tcgatttcga ctgttccgaa gggcgaacag gtcaaaatga
     3241 caagttttct cgccctgggt ggaaagttct gcacggggat tttagctctc atctcccgca
     3301 tggaattttt gtgacatttc aaaaaaaagc gcatcagaat cagcctgaca cgctttgttt
     3361 ttgtcaaaga ggatttcggg ttactgcgtt gctaatccgg tcacttcagc tgttgatgac
     3421 ggggatacag tcccgccctg aacatcccgg atatagtttg cctgacatgc agccagaata
     3481 atgactgcaa gaagcaataa aataatccct gttatttttt tcataacgac tccttgttgt
     3541 gaaagaatgc cagccattca tccataaaca accggcattc atattttagc cctgtttaaa
     3601 tcctggctta ccgttagcag ctcgccattc tttaatttct ttaacaatcc cttcaggact
     3661 atcttccctg tcatcgcgag gataataaat cagatctgaa ccatctgggt gctcagttaa
     3721 tcgctcaaac tctctcacta atttattgtc atcctcttca gtagcacctt cagctctaca
     3781 tatttttttt acaaactcca gaaattcagc ctcggtataa tcactaatac tatgtttcag
     3841 ttccattttt acttacctct atgaatatca atatgtcgct taggtgtggt cactctgata
     3901 ttatccatat catagacacc accatcctga ctgattggtt tatcatgatg taattcaaag
     3961 cgttccctac cacctacttg gtctttcttc cttgcaaaag gtgcttttcc cttttgaatg
     4021 ttcgtcttat tactgtcttt aaattgctta ctaagctcgg gatcttttga cacttcttcc
     4081 cagaatttct tccggaaatc gtcaaaactt ttaaattctt tatcacgcaa cttatcagca
     4141 atgcgatcag gaattggcgc tcctgaatct ttacccgcat catcaagcca tttatcacca
     4201 actggttttc ctttacccgt cgccttccct ggcttattcc gtttactctc cttatctaat
     4261 ttatccttag agtccttttc tttattttcc ttctgcttac ggcgttccag cgcagcacct
     4321 aacgcagcat cagcatcaga cttatctttt gcagcagcat caaatgcagc cttcttatta
     4381 tttacatccg tctgtgcccg ctgggcctta agccctgcca tttgccacat tcggtgacca
     4441 gcagccattg gttctcgggc aaatcgctcg aattgtttta tttcagcctt tgcatcagca
     4501 agagttttat tcgcagcatc aagttcactt ttacgagaat tataaacctg aacagcttta
     4561 gcctgtcgtt cctggtttct ggcaacatct ttattagcct gatccagttc tgcgcgggcc
     4621 tgttcataat tgcgttcagc cacttctaca ggatgtgtgt cattccattc ctgctggagg
     4681 cgattttcct catcctgacg ttgttttacc tgtgcagggg taagaacatc tgtcactgat
     4741 acataaatcg gcttctgccc actttctttc ggaaaacgga tcaccgcttc atgtgaacca
     4801 ccaccaaagg taaatcctgc aggaacggca gtccggcctt tatcctccgt aataccacgg
     4861 ggaagggttg ttgacaccgg aaggcctttg acagtgctga ctgtcagaga agggacacca
     4921 ggaaatgatg cgtgaaatac acctggagta cgtgttggtt tggcattcac aacaggcaca
     4981 ctcataggca cacctgcaac tacggcaata tgctgtcgtg tgtccttcac aacatctgtc
     5041 acacgtttcg tcacgctgac agttgcctgg tccagcggaa gtgtactgac ctgaacattc
     5101 gtcactgttt ctgccggtaa tgacgtcaca atctttgaca tcatattcgg gtcatctttt
     5161 gctatttcag atggcagaat gccgtaaagc gcaatacccc atgcgctgaa tttaaacgga
     5221 cctttcaggg ctgcaaagat atcagctatt gccgcagata aagcctcacc agagacggcg
     5281 atacccagcg ttccggcacc aggagttgcc aaagcaggaa aaccaaatgc cactgctgct
     5341 acggatgaat tgctgccacc accggaatta ccattccccc cgccattacc attgccggag
     5401 ccacctcccc agtgaacacc actaccggaa ccgccacccc atggattatt ttccgaactc
     5461 catcctgaac cgtcagaagc cccaccattt ccaccaagcc ccgtagggcc gccattaatg
     5521 ttaccacctg tgttatgtgc gccactgttg tggtcgcgtc catctccacc gctcataaaa
     5581 attccctctt ttaagcgtta aaacaattga ttacatagtt aatactgtac ataaaaccac
     5641 tggttttatg tacagatcaa aaagtactcg tcattatttt ttctgtcaag tcaggggggg
     5701 atattttttg gcagttcgga ggaatcactc atgaaaacgg tgaacggaac gcgaaatcct
     5761 ttcgcgggtc gtgaggcgtt ggacaagcga agcgcgtcag tttttccggc tcttacccgt
     5821 gacaggtttc tccaccagtt tatcttccag tgtcacaact ttctgataca ccgccgctgt
     5881 ggcgttgtag ttcctgtcga gtattgtttt cattccccat ccgagcacac agaacataac
     5941 cagaaaagcc actgacaccc cacccagcca tttccagcta taccctttaa gggtgttgta
     6001 ttcctgcacg atattgctgt gaattgtcgc aatatccctg ttcagggcat ccgcgtttgc
     6061 cttcatgttt gcgactgact cttccagccg ctgaaaatcc tggctgatgc gctcacctat
     6121 cagtcctgcc acgtccagct tctgcacctc atttctgact tcatccctga cctgttttgc
     6181 cacaccgtca gaaatatccc gatatccgcg aagtgtttcc cttgccacag
//
