LOCUS       CP099200                4073 bp    DNA     circular BCT 13-APR-2023
DEFINITION  Escherichia coli strain RHB20-E3-C07 plasmid unnamed3, complete
            sequence.
ACCESSION   CP099200
VERSION     CP099200.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15488564
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB20-E3-C07
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 163x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/15/2022 19:54:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,838
            CDSs (total)                      :: 4,718
            Genes (coding)                    :: 4,595
            CDSs (with protein)               :: 4,595
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 64 of 123
            Pseudo Genes (incomplete)         :: 64 of 123
            Pseudo Genes (internal stop)      :: 36 of 123
            Pseudo Genes (multiple problems)  :: 37 of 123
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB20-E3-C07"
                     /isolation_source="Pooled sediment sample collected from
                     floor of cattle farm"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(645..839)
                     /locus_tag="NFK00_24175"
     CDS             complement(645..839)
                     /locus_tag="NFK00_24175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WFX35552.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQVRFETESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1410..1601
                     /locus_tag="NFK00_24180"
     CDS             1410..1601
                     /locus_tag="NFK00_24180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WFX35553.1"
                     /translation="MCLMGTVCAQTVRHSFSCIAMQWAENDPDAFFRFSLGKTSLFAVS
                     ASESRSVRFSGARNATLW"
                     /name="hypothetical protein"
     gene            complement(1612..2205)
                     /locus_tag="NFK00_24185"
     CDS             complement(1612..2205)
                     /locus_tag="NFK00_24185"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="WFX35554.1"
                     /translation="MSGRKFGRLPNWWARSSWLVNNVRSNEIGGGIASMKCLLALSVLI
                     DFHSRTASVSFTGMERLTGLSRPMIVKGVAKLEKDGLIKIDREMFVNSYELTVQPNDDR
                     WAKVPVDTIRKKLNTISNRGMAPFVALKLYLTIISLRYQSNNTVKVTHETLRSYTGVQP
                     NQIRFGLDVLYCSRLIHLQPKEDRESNIYEIIGL"
                     /name="replication protein"
     gene            complement(2799..3713)
                     /locus_tag="NFK00_24190"
     CDS             complement(2799..3713)
                     /locus_tag="NFK00_24190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WFX35551.1"
                     /translation="MPNTIHYPHVIPFISQGKINAIKSTFGNNLSDRECYGIYIWSQKA
                     SSAIYPLLQQLEVTLRNSIDKEATKLIGQKWWDNVYTDTSKSKHGDFIHNINKAKRRYE
                     NEFKKKNPSMANTKIIAPHDDIIAHTDFYTWQAVLSDAFHTQSRSEASRALWPRLTYRV
                     LKGLDRSKDEGTARIDFLNELNEIRNYRNRLSHNDCIWIKIHSKNLQSAVETIREKINK
                     IEGLIKTINPQVHLSLTKWGSFYHAKRICSQKEAELHLGKGIINSTTDEMNTILDQLYA
                     LTADGKLTGVVKRNTNNIAFHKF"
                     /name="Abi family protein"
     Typing          478..564
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.43e-38"
     Typing          876..974
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.0"
                     /coverage="100.0"
                     /evalue="6.26e-40"
     Typing          1099..1354
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.411"
                     /coverage="89.0"
                     /evalue="2.67e-103"
     Typing          complement(2..467)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.03"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3944..4073)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.656"
                     /coverage="95.0"
                     /evalue="9.58e-50"
ORIGIN
        1 gacctacacc gaactgagat accaacagcg tgagctatga gaaagcgcca cgcttcccga
       61 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
      121 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
      181 acttgagcgt cggttttttt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
      241 cggcgcggcc ttttgtcctg gctttgcctt gttgctcaca tgttctttcc ggtcttatcc
      301 cctgattctg tggataactg tgttaccgtt tttgtgtgag tcagtaccgc tcgccgcagc
      361 cgaacgaccg agcgtagcga gtcagtgagc gaggaagcgg aaaagcgcct ggacgtgcat
      421 tttctcctta cgcatctgtg cggcatttca cacccggcat ggggcacttt tcatacaatc
      481 cgcactgatg ccgcatggtt aagccagtat acactccgct atcgctacgt gactgggtca
      541 tggctgcgcc ccgacacccg ctaaaacccc ctgacgcgcc ctgacgggct tgtctgctgc
      601 cgaaaacgct cgcggacaag ccgtgagcgc ctccgtcagc ttcgttatgt tcctgtttca
      661 ctttcggtct caaagcgaac ctggatgctg ttctggagtt cttccgcgag ttcgtgcagt
      721 cgttcgcaca tggctgcctg ttcgtcggca tccagagcat ccagtttttc aagcagtgtc
      781 aggctctggc ttttgataaa tcgcgccata ttcagcgcag tttgttgttg tttgttcacc
      841 ttccgttgtt ctccgtcagt tccgggtgtc ggggggaccc tgagcagctg gtaattgtaa
      901 gacgttgcgg gcgacggtat tacaattgca catcctgtcc tattttttta ggacgtatca
      961 tggcacagca tatcaactga taaataatcc ctgtgcagtg cagccgctgt tgcataaaat
     1021 ttcgctctgc gtcgagtgtt tcttaatcaa taattccgct gatactccgg aaaaacgcga
     1081 gtctgttctc attcctgaaa tgcccacacc ccacgcaaaa acaagttttt gctgattttt
     1141 ctttataaat agagagttat gaaaaattag tttctcttgc tctctttgcg atatttaaaa
     1201 aagcggtgcc ggcgcagcta caacaccgcg ccgacaccgc tttgtagggg tggtactgac
     1261 tatttttata aaaaacatca ttttatatca ggggtgctgc tagcggcgcg gtgtgttttt
     1321 ttacaggacg cccctggggg cgctgccagg ggtgtctgtt cagatatgta gcaatgagtc
     1381 agggattcac gaactgaaaa tgtccgaaaa tgtgtctcat gggcaccgta tgcgcccaaa
     1441 ccgttcgtca ttcattttca tgcatagcta tgcagtgggc tgaaaacgat cctgacgcat
     1501 tttttcggtt ttcactgggt aaaacatctc tttttgcggt atctgcgtca gaatcgcgtt
     1561 cagtgcgttt tagcggtgcg cgtaatgcga cgttatggta aatgcatttc ctcataggcc
     1621 gattatttca tagatgtttg actctctatc ttcttttggt tgcagatgta tcaggcgact
     1681 acagtacagt acatctaagc cgaacctaat ctggtttggc tggacacctg tatagcttct
     1741 cagtgtttca tgtgtcacct taacagtatt atttgactga taacgaaggc ttataattgt
     1801 taaatatagt tttaatgcca caaatggggc catccccctg ttcgatatgg tgtttagttt
     1861 tttcctgata gtatctactg gtacttttgc ccacctatca tcatttggct gtactgttaa
     1921 ttcataggag ttaacaaaca tctctctatc tatctttata agaccatctt tctcaagttt
     1981 ggctactcct ttcactatca tggggcggct tagtccagtc agtctttcca ttccggtaaa
     2041 tgatactgag gctgttcttg aatggaaatc aatcaagacg gatagagcta acaagcattt
     2101 cattgatgca atgccgcccc ctatctcatt tgaacgaaca ttgtttacaa gccatgagct
     2161 tcgggcccac cagtttggaa gcctgccaaa ttttctaccg ctcattttta ccccattttc
     2221 ggtcaaggag agatataaat agctttatta tcatatacat gaaaaccatt gcacctaaag
     2281 atacaacaga aaccaaaacc tccggctttg cggaagttag cgttaccagc aaattgttga
     2341 gggtttccaa ttggtcctcc agtcgcatac taagttgttg tgttttaact ttatatacgc
     2401 ttattagaat aaatctagat aaagcaatat ttggtggctg cttgttttcc catataaaac
     2461 atgatgttat cttgttttta tcgtttttta actggtaaat gtaaatttga taatgctggt
     2521 ttaatcggcg tttttagtta tttttgttgt gtgttttttg atctattggt aaatggtgga
     2581 tggtaagctt tggcgtgatc atgggaaata gtttgctctt tttcctgatg atgatgtatc
     2641 atgtatggca gtaaagcctt gagctttcgc tttaatgcat tgtgaaattt ttatagattc
     2701 acaatgacat taaggccacg ctccaccgaa aggtgataat ggcaagagat ttggatgagg
     2761 ctcgacagga aactgtcggg ccttttctat tttgtgatct aaaacttgtg aaatgcgata
     2821 ttgttggtat ttcgttttac aacacccgtt agctttccgt cagcagttag tgcgtatagt
     2881 tgatctagaa ttgtgttcat ctcatctgtt gtgctattga ttattccttt gcctagatgc
     2941 aattcggcct ctttttgaga acatattctt ttggcgtgat aaaaggaacc ccacttcgtt
     3001 aatgataggt gtacctgtgg gtttatcgtt tttatcagtc cttctatttt gtttattttt
     3061 tctcttattg tttctactgc agattgcaaa tttttagagt gtatttttat ccatatgcaa
     3121 tcattatgcg ataacctatt tctgtagttt ctgatctcat tcagctcgtt taagaaatca
     3181 attctggctg tgccttcatc tttgcttcta tctaatccct tcagtactct gtaggtgagc
     3241 ctgggccata aggctcttga tgcttcagat cttgattgtg tatgaaaagc atcgctcaaa
     3301 accgcttgcc atgtgtaaaa atctgtgtgt gcaatgatgt catcatgagg tgcaattatt
     3361 ttggtatttg ccattgatgg gttttttttc ttgaattcat tctcatatct tctttttgct
     3421 ttgtttatat tatgtatgaa gtcaccgtgt ttgctttttg atgtgtcggt gtatacatta
     3481 tcccaccatt tttggcctat tagttttgtc gcttctttat caatcgagtt tctcaaagta
     3541 acttcgagtt gttgcagtaa tggatatatg gcagatgagg ctttctggct ccatatatat
     3601 atcccgtagc actccctatc agacaggtta ttaccaaacg tgctttttat agcatttatt
     3661 tttccttggg atataaatgg tattacatga gggtaatgaa ttgtatttgg catatccatc
     3721 tctatcatac acttagtggt gattggatgt tgccatacac gtatggatag ttgtagagtg
     3781 ttaaacgaaa aaaccgcctt ggcgggcggt ttttcgaagg ttcaaagagt tggcgctctg
     3841 tgaaccaagg taactggctt ggaggagcgc agtcaccaaa atctgttctt tcagtttagc
     3901 cttaacaggt gcataacttc aagacaaact cctctaaatc agttaccagt ggctgctgcc
     3961 agtggcgtta aggcgtgcct taccgggttg gactcaagac gatagttacc ggataaggcg
     4021 tagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aac
//
