LOCUS       CP099177                3257 bp    DNA     circular BCT 13-APR-2023
DEFINITION  Escherichia coli strain RHB23-SO-C02 plasmid unnamed4, complete
            sequence.
ACCESSION   CP099177
VERSION     CP099177.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15488584
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB23-SO-C02
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 140x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/15/2022 19:06:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,721
            CDSs (total)                      :: 4,605
            Genes (coding)                    :: 4,498
            CDSs (with protein)               :: 4,498
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 107
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 48 of 107
            Pseudo Genes (incomplete)         :: 53 of 107
            Pseudo Genes (internal stop)      :: 31 of 107
            Pseudo Genes (multiple problems)  :: 24 of 107
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB23-SO-C02"
                     /isolation_source="Pooled soil sample collected from floor
                     of sheep farm"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(3071..3257,1..110)
                     /locus_tag="NFK20_23595"
     CDS             join(3071..3257,1..110)
                     /locus_tag="NFK20_23595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WFY02122.1"
                     /translation="MINLVDPVHPGVFIREMFMEPFEISAADLSEKLHVSPSTLSRVLN
                     GKADLSVEMALRLSRVLGRSAESWINMQTQYSLNASRKSPNVIKELDELVPIF"
                     /name="HigA family addiction module antitoxin"
     gene            complement(1107..1301)
                     /locus_tag="NFK20_23600"
     CDS             complement(1107..1301)
                     /locus_tag="NFK20_23600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WFY02123.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQVRFETESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2445..2726)
                     /locus_tag="NFK20_23605"
     CDS             complement(2445..2726)
                     /locus_tag="NFK20_23605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WFY02121.1"
                     /translation="MIINIKHKGLRKFAETGNNAGIPQQFTQRLKLILTALSTATNLQD
                     MMDLKALRCHPLKGDRQGEYSLEVNGNWRVVFKLEEPNVFDVDLEDYH"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     Typing          952..1034
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.619"
                     /coverage="100.0"
                     /evalue="8.2e-33"
     Typing          1338..1436
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.22e-45"
     Typing          1561..1816
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.124"
                     /coverage="89.0"
                     /evalue="5.65e-115"
     Typing          complement(382..939)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.464"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtcgcagtgc cgagtcgtgg ataaacatgc agactcaata cagtcttaac gcgagcagga
       61 agtcacctaa tgtcattaag gaattagatg aattggttcc tatattttga gaaaagaaaa
      121 ggccgctata tgcggccttt ttctgtcttg actctcaggc aactggatcg tatcgtttct
      181 cgtaaacgaa aaaaccaccc ggcaaggtgg ttttttcgaa ggttcagtaa ccggcaagtt
      241 tctgaaccag ggtaactggc ttggaggagc gcagtcacca aaacttgttc tttcagttta
      301 gccttaactg gcgcataact tcaagactaa ctcctctaaa tcagttacca gtggctgctg
      361 ccaatggtgt tttgcatgtc tttccgggtt ggactcaaga tgatagttac cggataaggc
      421 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
      481 cactgaaccg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
      541 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagcc
      601 accaggggga aacgtctggt atctttaagt cctgtcgggt ttcgccacct ctgacttgag
      661 cgtcgatttc tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc ctgcggcgcg
      721 gccttcttcc tgttcttggt cttttgctca catgttcttt ccggccttat cccctgattc
      781 tgtggataac cgtgctgccg tttttgtgta agtcagtacc gctcgccgca gccgaacgac
      841 cgagcgtagc gagtcagtga gcgaggaagc ggaaaagcgc ctggacgcgc attttctcct
      901 tacgcatctg tgcggaattt cacacccggc atggggcact tttcatacaa tctgcactga
      961 tgtcacatag ttaagccagt atacactccg ctatcgctac gtgactgggt cagggcttcg
     1021 ccccgacacc cgctgacgcg ccctggcggg cttgtctgct gccgaaaacg ctcgcggaca
     1081 agccgtgagc gcctccgtca gcttcgttat gttcctgttt cactttcggt ctcaaagcga
     1141 acctggatgc tgttctggag ttcttccgcg agttcgtgca gtcgttcgca catggccgcc
     1201 tgttcgtcgg catccagagc atccagtttt tcaagcagtg tcaggctctg gcttttgata
     1261 aatcgcgcca tattcagcgc agtttgttgc tgtttgttca ccttccgttg ttctccgtca
     1321 gtaccgggtg tcgggtggac cctgaccagg tggtaattgt aagacgttgc gggcgacggt
     1381 attacaattg cacatcctgt cctgtttttt taggacgtat catggcacag catatcaact
     1441 gataaataat ccctgtgcag tgcagccgct gttgcataaa atttcgctct gcgtcgagtg
     1501 tttcttaatc aataattccg ctgatactcc ggaaaaacgc gagtctgttc tcatgcctga
     1561 aatgcccaca ccccacgcaa aaacaatttt ttgctgattt ttctttataa atagagagtt
     1621 atgacaaatt agttcttctt gctctctttg tgatatttaa aaaagcggtg tcggcgcggt
     1681 gttgtagctg cgccaacacc gctttttagg ggtggtactg actattttca taaaaaacat
     1741 cattttatat taggggtgct gctagcggcg cggtgtgttt ttttacagga cgcccctggg
     1801 ggcgctgcca ggggtgtctg ttcagatatg tagcaatgag tcagggattc acgaactgaa
     1861 aatgtccgaa aatgtgtctc atgggcaccg tatgcgccca aaccgatcgc cattcacttt
     1921 catgcatagc tatgcagtga gctgaaagcg atcctgacgc atttttccgg ttttcgcttg
     1981 gtaaaacatc tctttttgcg gtgttcgcgg cagaatcgcg ttcagcgcgt tttagcggtg
     2041 cgcgtaatgc gacgttatgg taaatgctgt gatttgcggg atggttttat ctatgttgat
     2101 atcaatacca cctcttatgg aggtggctta caccgatttt tccttgttgt ctgtttcaga
     2161 acagatagag gcttttttgg caattatcca accactattg atagtgttta ttactactta
     2221 gataactgag cctttactgt atctgcccct gcaagcagga acccaacagt taagaaccac
     2281 ccccagccat tgataccttt tagcgctaga attgctgcgc aaatgatggc tgctataggg
     2341 actatctggt acagaaaata cttcatataa cttaccccga ggttttgctt ttttatgaga
     2401 ccctaaagca tcgatactgt cactttgatg ctttaaggac cttcttagtg ataatcctcg
     2461 agatccacgt caaagacgtt aggttcttcg agcttaaaca ctacgcgcca gtttccattg
     2521 acctctaacg aatactctcc ttgacggtct cccttcaatg gatggcagcg caatgccttt
     2581 aaatccatca tatcctgcaa gttcgtcgct gtgcttaagg ctgttaggat cagcttcaga
     2641 cgttgagtaa actgttgcgg aattcctgcg ttgttacctg tttcggcaaa cttacgcaaa
     2701 cctttatgct tgatatttat gatcattaat caccatccca tatttgttta gatagaaaca
     2761 tcgcagttta tttttccggt tcatccgtat ttccattgca aaccccttgc tctatttgaa
     2821 taaatgacgt aggttttact taaccaagtc gcattttaat ctgttgacat tggtttgatg
     2881 cgctgtacaa tgccggggcc ttgaatttca catcaagtac ccacatcata aggaatgatt
     2941 agacgcacca atgtgcatca taatgtcatt ctttttttta gtcaacaatg agttgcgtta
     3001 tgcgcaatgc gcaatctgaa aacgcaatag gcctatcttt ttaaaactaa tataggcttc
     3061 agaggaaggt atgattaatc ttgtcgatcc agtgcatcca ggggttttta tccgtgagat
     3121 gtttatggaa ccgtttgaaa tatcagccgc ggacctgtct gaaaaactgc atgtatcacc
     3181 ttccactcta agtagagtgc tgaatggaaa agctgattta agtgttgaga tggcattaag
     3241 gctttctcga gttcttg
//
