LOCUS       CP099176                7198 bp    DNA     circular BCT 13-APR-2023
DEFINITION  Escherichia coli strain RHB23-SO-C02 plasmid unnamed3, complete
            sequence.
ACCESSION   CP099176
VERSION     CP099176.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15488584
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7198)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7198)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB23-SO-C02
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 140x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/15/2022 19:06:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,721
            CDSs (total)                      :: 4,605
            Genes (coding)                    :: 4,498
            CDSs (with protein)               :: 4,498
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 107
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 48 of 107
            Pseudo Genes (incomplete)         :: 53 of 107
            Pseudo Genes (internal stop)      :: 31 of 107
            Pseudo Genes (multiple problems)  :: 24 of 107
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB23-SO-C02"
                     /isolation_source="Pooled soil sample collected from floor
                     of sheep farm"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            join(6795..7198,1..34)
                     /locus_tag="NFK20_23540"
     CDS             join(6795..7198,1..34)
                     /locus_tag="NFK20_23540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WFY02114.1"
                     /translation="MGHCNFMAWHTLQDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRR
                     EFETSELIRAYGELKQNETQERHTVGQAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRHQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="entry exclusion protein 1"
     gene            115..534
                     /locus_tag="NFK20_23545"
     CDS             115..534
                     /locus_tag="NFK20_23545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WFY02115.1"
                     /translation="MRLTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSKLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(852..1085)
                     /locus_tag="NFK20_23550"
     CDS             complement(852..1085)
                     /locus_tag="NFK20_23550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WFY02116.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSRTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1092..1610)
                     /locus_tag="NFK20_23555"
     CDS             complement(1092..1610)
                     /locus_tag="NFK20_23555"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WFY02117.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKVLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMRQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(1648..2079)
                     /locus_tag="NFK20_23560"
                     /pseudo=""
     CDS             complement(1648..2079)
                     /locus_tag="NFK20_23560"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2288..2611)
                     /locus_tag="NFK20_23565"
     CDS             complement(2288..2611)
                     /locus_tag="NFK20_23565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WFY02118.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPLARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2676..2867
                     /locus_tag="NFK20_23570"
     CDS             2676..2867
                     /locus_tag="NFK20_23570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WFY02119.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3537..3650
                     /locus_tag="NFK20_23575"
     CDS             3537..3650
                     /locus_tag="NFK20_23575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WFY02120.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDKAPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(3868..4008)
                     /gene="crl"
                     /locus_tag="NFK20_23580"
     CDS             complement(3868..4008)
                     /gene="crl"
                     /locus_tag="NFK20_23580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin R system lysis protein"
                     /protein_id="WFY02111.1"
                     /translation="MKMILSGGMLVLMALLSGCQANYIRDVQGGTVAPSSSSELTGIAV
                     Q"
                     /name="crl"
     gene            4128..4652
                     /gene="cri"
                     /locus_tag="NFK20_23585"
     CDS             4128..4652
                     /gene="cri"
                     /locus_tag="NFK20_23585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_089619593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin R immunity protein"
                     /protein_id="WFY02112.1"
                     /translation="MQKEYTYNKDLNRMLFLITFAGIIPLIIIFITYTSNPNFYLIRII
                     FDNTQNIPSVISSYNPVMTKVMDIYGKSAPLLAFIAFIMLFKHTRTPKITDRKKIITAC
                     ILSPFLCIFYAYFFLWHNFELTTSGRPVRLMSENDLTLLIFYICLYYCSFFITYALCLQ
                     PVIAYKLWKER"
                     /name="cri"
     gene            complement(4680..6569)
                     /gene="cra"
                     /locus_tag="NFK20_23590"
     CDS             complement(4680..6569)
                     /gene="cra"
                     /locus_tag="NFK20_23590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin R"
                     /protein_id="WFY02113.1"
                     /translation="MVCFKSQRGMNMPGFNYGGYGDGTGWSSESGGPAPGGGMHGNSGG
                     QRGDNANSSNSVSQQISAIQNDQKLKQKVVNMLIAARKMNPEAKMILGSIAPSGVMQVT
                     IEGVTSTQARQLGLGGLVMGYNASGVIGAVGEIDTGHRLNASGASTPGSETSVESFVNG
                     QKPAGEWHAVAKDSWTGAGPVNVGLVNNAIKSVRIIKKGYVTGVLTPEEVMNKAEYKAM
                     RQAFDSLPLAKQGEAVRQIVAAWSLAYQDFPVNLKKEMGRVTERIVDAINLALILNQTE
                     SRLSESQKNVDVANQIISDTVKAINDVNKKIAEKRNQQVSLTDLMNKKQKEVEDLKKIF
                     KNHSYHRIRDAQREYDDARNKYALLASDINALQAQVSGLTARKQQAEQNKAAAEKAKAD
                     AAAKAAAEKAAAEAKAKAEAEKARKEAEEKANDEKAVLTKASEIIISVGDKAGEYLGDK
                     YKVLSREIADNIKNFQGKTIRSYDEAMASVNKLMANPDLKINAADRDAIVNAWKAFDAE
                     DMGNKFAALGKTFKAADYVMKANNVREKSIEGYQTGNWGPLMLEIESWVLSGIASAVAL
                     SFFSVAVGSLFITAGVSVTAVGLMGIIFAGFIGALIDDDFVDKLNNEIIRPAY"
                     /name="cra"
     Typing          complement(2953..3040)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.629"
                     /coverage="100.0"
                     /evalue="6.91e-34"
     Typing          3060..3609
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.928"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(749..2298)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.611"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acggggattc tggtccaggt tgttcggtcg ctgaacgctg tcagagactg atgataaaat
       61 agtcttcgga taataactca ccgagaataa atactttaag gtagggagac actcatgaga
      121 cttaccggaa acaaactttg tcttatcgcc atgataacag caacagtagc tctcacagcc
      181 tgtaccccaa agggcagcgt ggaacaacat acccggcatt acgtatatgc ttctgatgac
      241 ggttttgatc ccaacttttc cacccaaaaa gccgacacaa cacgaatgat ggtgcctttt
      301 tttcggcagt tctgggatat gggagctaaa gataaagcga caggaaaatc acggagtgat
      361 gtgcaacaac gcattcagca gtttcacagc caagaatttt taaactcact ccggggcaca
      421 actcaatttg cgggtactga ttaccgcagc aaagacctta ccccgaaaaa atccaagctg
      481 ctggctgaca cgatttctgc ggtttatctc gatggctacg agggaagaca gtaagtggat
      541 ttaccataat cccttaattg tacgcaccgc caaaacgcgt tcagcgcgat cacggcagca
      601 gacaggtaaa aatggcaaca aaccacccga aaaactgccg cgatcgcgcc tgataaattt
      661 taaccgcatg aatacctatg caaccagatg gtacagacca cattagcccg tcttaatcca
      721 cttaacctgc catttttcat agtttcacca tcccagcgaa ggaccatcca gtgtgcgttc
      781 ctgtatttcc agctggcgct cacgctctag ggataacgca tgttcgcgct cctgtatcag
      841 ccgttcctct cttatctcca gttctcgctg tataactggc tcaagcgttc tgtctgctcg
      901 ctcaagtgct gcacctgctg actcaactgc atgacccgct cgttcagcat cgcgttgtcc
      961 cgttgcgtac gcgaaaacat cttctgcaat tccacgaagg cgctctccca ttcgctcagc
     1021 cgctgcatat agtcctgttg cagctgctct aaggcgttca gcaaatgtgt ttccagctct
     1081 gtcattctgt gtcactcctt cagatgtacc cactctttcc cctgaaaggg aatcacctct
     1141 gctgatttcc cgtacggaag gaccagaaat ttcctgttcc cgtcctgcac gaactccacg
     1201 ccccatgtct tcgcgttcag tttctgcaat gtctcttcct gcttcctgat atcttccagg
     1261 ttcgcctgta tcctcccgcc aagataccag agcgtcccgc cactcgcggt aaacaggaga
     1321 aagactatcc ccagtaacat catgcccgta ttccctgcca gctttaacac gtccttcctg
     1381 tgctgcctca tcgcctcttt caccccttcc cggtgtttct ccagcgattc ctctgtcgag
     1441 gctgtgaaca gggctatagc gtctctgatt ttcgtctcgt ttgatgtcac agcctcgctt
     1501 acagattcgc cgagcctcct gaactcgttg ttcagcactt tctctgtaga ttcggctctc
     1561 tctttcagct ttttctcgaa ctccgcgccc gtctgcaaaa gattgctcat aaaatgctcc
     1621 tttcagcctg atattcttcc cgccgttcgg gtctgcaatg ctgatgctgc ttcgcgtcac
     1681 cctgacaact tcaagccccg cctctgtgag cgcctgaatc acatcctgac ggccttttat
     1741 ctcccctaca tgataaagag cgtctatccc gcgtgtgacg ctctcagcaa gcgcctgttt
     1801 cgtttccggc aggttatcag agagagtcag tatccggcgg ttctccgggg cgttggggtc
     1861 atgcagcccg taatggtgat tcaccagcgt ctgccaggca tcaattctcg gcctgtctgc
     1921 ccggtcgtag tacggctgga gacgttttcc ggtctgtagc tccatgttcg gaatgacaaa
     1981 attcagctca agccgtccct tgtcctggtg ctccacccac aggatgctgt actgattctt
     2041 ttcgagacct ggcatcagta cgcgctcaaa gctcgccatc actttttcac gtcctcccgg
     2101 cggcagctcc ttctccgcga acgacagaac gcctgacgtg tatttcttcg caaatggcgt
     2161 ggcatcgatg agttcccgga cttcttccgg attaccctga agcaccgttg cgccttcgcg
     2221 gttacgctcc ctccccagca ggtaatcaac cggaccactg ccaccacctt ttcccctggc
     2281 atgaaattta actatcatcc cgcgccccct gttccctgac ggccagacgc agccggcgca
     2341 gctcatcccc gatggccatc agtgcggcca ccacctgaac ccgatcaccg gaagaccact
     2401 gcccgctgtt caccttacgg gctgtctgat tcaggttatt tccgatggcg gccagctgac
     2461 gcagtaacgg cggtgccagt gtcggcagtt tcccggaacg ggcaagcggc tcccccaggc
     2521 agacccgccg catccatacc gccagttgtt taccctcaca gcgttcaagt aaccgggcat
     2581 gttcatcatc agtaacccgt attgtgagca tcctctcgcg tttcatcggt atcattaccc
     2641 catgaacaga aatcccccct tacacggagg catcagtgac taaacaggaa aaaaccgccc
     2701 ttaacatggc gcgctttatc agaagccaga cattaacgct gctggaaaag ctcaacgaac
     2761 tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgcc gatgagcttt
     2821 accgcagctg cctcgcacgt ttcggggatg acggtgaaaa cctctgacac atgcagctcc
     2881 cggagacgga cacagcttgt ctgtgagcgg atgccgggag ccgacaagcc cgtcagggcg
     2941 cgtcagcagg ttttagcggg tgtcggggcg cagccctgac ccagtcacgt agcgatagcg
     3001 gagtgtatac tggcttagat atgcagcatc agtgcggatt gtaagaaaac agcgccatat
     3061 acggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc
     3121 ttcctcgctc actgactcgc tacgctcggt cgttcggctg cggcgagcgg tgcaagctca
     3181 ctcaaaggca gtaatacggt tatccacaga atcaggggat aaggccggaa agaacatgtg
     3241 agcaaaaagg caaagggcaa cgaaaggcct cgccgctggc gtttttccat aggctccgcc
     3301 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     3361 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     3421 tgccgtttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     3481 gctcacgctg ttggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     3541 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     3601 acccggtaag acacgacaaa gcgccactgg cagcagccac tggtaactga tttagaggag
     3661 ttggtcttga agttatgagc cggttaaggc taaactgaaa agacaggttt tggtgactgt
     3721 gctcctccaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     3781 ccaccgttgg tagcggtggt ttttttgttt gcaagcagaa gattacgcgc agaaaaaaag
     3841 gatctcaaga agatcctttg atcttttcta ctgaaccgcg attcccgtca gttcagaaga
     3901 ggaggatggt gcgacggtcc ctccctgaac atcgcgaata taattagcct ggcagcctga
     3961 tagcaaagcc atcaagacaa gcatccctcc tgataatatc attttcatat atcccctcat
     4021 taactcatac ccctaatatt agggtgacca ttactcgcac agcatgatat gcttatacac
     4081 atcaacaact aaattaataa caatgaaaaa ccgggagtat tataccaatg caaaaagagt
     4141 acacttacaa caaagattta aacaggatgc tttttttgat tactttcgcg ggtataatcc
     4201 cattaattat tatttttata acttatacat caaaccccaa cttctattta atccgcatca
     4261 ttttcgataa tactcaaaat ataccttcag taatttcttc atataaccct gtgatgacaa
     4321 aggtgatgga tatctacggt aagagcgcac cattattagc atttatagct ttcatcatgc
     4381 tattcaaaca cacaagaacc cccaaaataa cagacagaaa aaaaatcata acagcttgta
     4441 tactgtcccc ctttttgtgt attttttatg cttatttttt cttgtggcat aacttcgagt
     4501 taacaacatc gggaagacct gttcgattga tgtctgagaa cgatcttact ttgcttatct
     4561 tttatatctg cctgtattat tgctcttttt tcataactta cgccctctgc ctccaaccag
     4621 taatagcata taaactctgg aaagaaaggt agtcctcctg actacctttc gactctcaat
     4681 taatacgcag ggcgaataat ttcattattt aacttatcaa caaaatcatc atcaatcagc
     4741 gcaccgataa agccagcaaa aataatgccc ataagtccca cagccgttac agacacgccc
     4801 gctgtaataa ataatgaacc aacagcgact gagaaaaatg atagcgccac agcagaagca
     4861 ataccactca atacccagga ctcaatctcc agcatcagcg gcccccagtt tccggtctga
     4921 tacccctcga tactcttctc ccggacgttg tttgccttca tcacataatc tgcggcttta
     4981 aacgtcttac ccagcgcggc aaacttattc cccatatcct ctgcatcaaa tgctttccag
     5041 gcattcacaa ttgcatccct gtcggcggca ttaattttaa gatcaggatt agccatcagt
     5101 ttattgacag aagccatcgc ctcatcataa ctacgaatcg ttttgccctg aaagtttttg
     5161 atattatctg ctatctcacg agaaagaact ttatatttat cacccagata ctccccagcc
     5221 ttatcaccaa cactgatgat aatttcactg gcttttgtca gaacagcctt ctcgtcattt
     5281 gctttttcct ctgcttcctt tctggctttt tcagcttcag cctttgcttt cgcttcagca
     5341 gccgcttttt ctgctgcagc tttcgccgct gcatctgcct ttgctttttc tgctgctgcc
     5401 ttgttctgtt ctgcctgctg tttccttgcc gtcagtccgg aaacctgtgc ctgcagcgca
     5461 ttaatatcac tggccagtaa agcatattta tttcgggcat catcatattc cctctgggca
     5521 tcacgaatac ggtgataaga gtgatttttg aatatctttt taagatcttc gacctctttt
     5581 tgttttttat tcatcaaatc agtcagtgat acctgctgat ttcgtttttc tgctattttc
     5641 ttatttacat cattaatagc ttttactgta tcactgataa tctggttcgc tacatcaaca
     5701 ttcttttgtg attccgacaa acgggactct gtctgattaa gaatgagcgc caggttaata
     5761 gcatcaacaa tccgttcagt gacacgcccc atctcttttt tcagatttac cggaaaatcc
     5821 tgatatgcca gactccaagc agcaacaatc tgtctgacag cctctccctg ttttgccagt
     5881 ggaagggaat cgaaagcctg ccgcattgct ttgtactctg ctttattcat cacctcttct
     5941 ggcgtgagaa cgcccgtcac atagcctttt ttgataatcc ggacactttt gatggcgtta
     6001 ttaaccagcc ctacattcac tggaccagcc cctgtccagc tgtccttcgc cacagcatgc
     6061 cattctccgg ctggtttctg tccgttcaca aaactctcca cagaagtttc gcttccggga
     6121 gtacttgctc ctgatgcatt aaggcggtga ccggtgtcta tttcgcccac agccccaata
     6181 acaccagaag cgttatatcc catgaccagc cctcccaaac caagctgcct ggcctgagtg
     6241 gaggttaccc cctcaatggt gacctgcata acaccagaag gagcaatact tcccagaatc
     6301 atttttgctt caggattcat ttttctcgca gcaatcagca tattcaccac tttctgttta
     6361 agtttctgat cattctgaat ggcagaaatc tgctgactca cagaattgct actgtttgcg
     6421 ttatctccgc gctggcctcc actatttcca tgcataccac cgcctggagc tggaccacca
     6481 ctttcagaac tccagccagt accgtcacca tacccaccgt aattaaaccc cggcatattc
     6541 attcctcttt gacttttaaa acaaaccatt aaaaacaaat actgtacaca taaccactgg
     6601 ctttatgtac agtataaaag ctacgccgct gcattttccc tgtcaagagt gtggacttcc
     6661 atttttgtga aaacgatcaa aaaaacagtc tctcacacca cgcgctattc ccgccggaaa
     6721 ccacaaaaac cggcgcaaac attaccgttc tcagacctca ttatgtttta ctgaaactat
     6781 gagatgagac acctatggga cactgtaact ttatggcatg gcacacactc caggacgcac
     6841 tgaaaatgac tggaagatcg cgttcacagt tttaccgtga tatgcgctca ggtcttgtca
     6901 gttaccgcac cagccaggac ggacgaaggg agtttgaaac cagtgaactg atccgggcat
     6961 acggcgaatt aaagcaaaat gagacacaag aaaggcacac tgtgggacag gcagaaaatc
     7021 cacatgatca gcagacagaa cgcattctcc gggaactgaa tgagctgaaa caatgcctga
     7081 cgctgatgct tgaggataaa caggcacagg atatggatcg cagacaccag gaagcagaac
     7141 gggaacagct acaaaatgag atagcccagc tcaggcaggc actggaactg gaaaagaa
//
