LOCUS       CP099172                3257 bp    DNA     circular BCT 13-APR-2023
DEFINITION  Escherichia coli strain RHB23-SO-C06 plasmid unnamed3, complete
            sequence.
ACCESSION   CP099172
VERSION     CP099172.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15488586
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB23-SO-C06
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 124x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/15/2022 18:26:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,720
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,497
            CDSs (with protein)               :: 4,497
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 107
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 47 of 107
            Pseudo Genes (incomplete)         :: 53 of 107
            Pseudo Genes (internal stop)      :: 32 of 107
            Pseudo Genes (multiple problems)  :: 24 of 107
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB23-SO-C06"
                     /isolation_source="Pooled soil sample collected from floor
                     of sheep farm"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            240..434
                     /locus_tag="NFK22_23590"
     CDS             240..434
                     /locus_tag="NFK22_23590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WFY11163.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQVRFETESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1431..1727)
                     /locus_tag="NFK22_23595"
     CDS             complement(1431..1727)
                     /locus_tag="NFK22_23595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WFY11164.1"
                     /translation="MINLVDPVHPGVFIREMFMEPFEISAADLSEKLHVSPSTLSRVLN
                     GKADLSVEMALRLSRVLGRSAESWINMQTQYSLNASRKSPNVIKELDELVPIF"
                     /name="HigA family addiction module antitoxin"
     gene            2072..2353
                     /locus_tag="NFK22_23600"
     CDS             2072..2353
                     /locus_tag="NFK22_23600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WFY11162.1"
                     /translation="MIINIKHKGLRKFAETGNNAGIPQQFTQRLKLILTALSTATNLQD
                     MMDLKALRCHPLKGDRQGEYSLEVNGNWRVVFKLEEPNVFDVDLEDYH"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     Typing          complement(106..204)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.22e-45"
     Typing          complement(508..590)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.619"
                     /coverage="100.0"
                     /evalue="8.2e-33"
     Typing          complement(2983..3238)
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.124"
                     /coverage="89.0"
                     /evalue="5.65e-115"
     Typing          603..1157
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.614"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gcgtttttcc ggagtatcag cggaattatt gattaagaaa cactcgacgc agagcgaaat
       61 tttatgcaac agcggctgca ctgcacaggg attatttatc agttgatatg ctgtgccatg
      121 atacgtccta aaaaaacagg acaggatgtg caattgtaat accgtcgccc gcaacgtctt
      181 acaattacca cctggtcagg gtccacccga cacccggtac tgacggagaa caacggaagg
      241 tgaacaaaca gcaacaaact gcgctgaata tggcgcgatt tatcaaaagc cagagcctga
      301 cactgcttga aaaactggat gctctggatg ccgacgaaca ggcggccatg tgcgaacgac
      361 tgcacgaact cgcggaagaa ctccagaaca gcatccaggt tcgctttgag accgaaagtg
      421 aaacaggaac ataacgaagc tgacggaggc gctcacggct tgtccgcgag cgttttcggc
      481 agcagacaag cccgccaggg cgcgtcagcg ggtgtcgggg cgaagccctg acccagtcac
      541 gtagcgatag cggagtgtat actggcttaa ctatgtgaca tcagtgcaga ttgtatgaaa
      601 agtgccccat gccgggtgtg aaattccgca cagatgcgta aggagaaaat gcgcgtccag
      661 gcgcttttcc gcttcctcgc tcactgactc gctacgctcg gtcgttcggc tgcggcgagc
      721 ggtactgact tacacaaaaa cggcagcacg gttatccaca gaatcagggg ataaggccgg
      781 aaagaacatg tgagcaaaag accaagaaca ggaagaaggc cgcgccgcag gcgtttttcc
      841 ataggctccg cccccctgac gagcatcaca gaaatcgacg ctcaagtcag aggtggcgaa
      901 acccgacagg acttaaagat accagacgtt tccccctggt ggctccctcg tgcgctctcc
      961 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     1021 gctttctcat agctcacgct gtaggtatct cggttcagtg taggtcgttc gctccaagct
     1081 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatca
     1141 tcttgagtcc aacccggaaa gacatgcaaa acaccattgg cagcagccac tggtaactga
     1201 tttagaggag ttagtcttga agttatgcgc cagttaaggc taaactgaaa gaacaagttt
     1261 tggtgactgc gctcctccaa gccagttacc ctggttcaga aacttgccgg ttactgaacc
     1321 ttcgaaaaaa ccaccttgcc gggtggtttt ttcgtttacg agaaacgata cgatccagtt
     1381 gcctgagagt caagacagaa aaaggccgca tatagcggcc ttttcttttc tcaaaatata
     1441 ggaaccaatt catctaattc cttaatgaca ttaggtgact tcctgctcgc gttaagactg
     1501 tattgagtct gcatgtttat ccacgactcg gcactgcgac caagaactcg agaaagcctt
     1561 aatgccatct caacacttaa atcagctttt ccattcagca ctctacttag agtggaaggt
     1621 gatacatgca gtttttcaga caggtccgcg gctgatattt caaacggttc cataaacatc
     1681 tcacggataa aaacccctgg atgcactgga tcgacaagat taatcatacc ttcctctgaa
     1741 gcctatatta gttttaaaaa gataggccta ttgcgttttc agattgcgca ttgcgcataa
     1801 cgcaactcat tgttgactaa aaaaaagaat gacattatga tgcacattgg tgcgtctaat
     1861 cattccttat gatgtgggta cttgatgtga aattcaaggc cccggcattg tacagcgcat
     1921 caaaccaatg tcaacagatt aaaatgcgac ttggttaagt aaaacctacg tcatttattc
     1981 aaatagagca aggggtttgc aatggaaata cggatgaacc ggaaaaataa actgcgatgt
     2041 ttctatctaa acaaatatgg gatggtgatt aatgatcata aatatcaagc ataaaggttt
     2101 gcgtaagttt gccgaaacag gtaacaacgc aggaattccg caacagttta ctcaacgtct
     2161 gaagctgatc ctaacagcct taagcacagc gacgaacttg caggatatga tggatttaaa
     2221 ggcattgcgc tgccatccat tgaagggaga ccgtcaagga gagtattcgt tagaggtcaa
     2281 tggaaactgg cgcgtagtgt ttaagctcga agaacctaac gtctttgacg tggatctcga
     2341 ggattatcac taagaaggtc cttaaagcat caaagtgaca gtatcgatgc tttagggtct
     2401 cataaaaaag caaaacctcg gggtaagtta tatgaagtat tttctgtacc agatagtccc
     2461 tatagcagcc atcatttgcg cagcaattct agcgctaaaa ggtatcaatg gctgggggtg
     2521 gttcttaact gttgggttcc tgcttgcagg ggcagataca gtaaaggctc agttatctaa
     2581 gtagtaataa acactatcaa tagtggttgg ataattgcca aaaaagcctc tatctgttct
     2641 gaaacagaca acaaggaaaa atcggtgtaa gccacctcca taagaggtgg tattgatatc
     2701 aacatagata aaaccatccc gcaaatcaca gcatttacca taacgtcgca ttacgcgcac
     2761 cgctaaaacg cgctgaacgc gattctgccg cgaacaccgc aaaaagagat gttttaccaa
     2821 gcgaaaaccg gaaaaatgcg tcaggatcgc tttcagctca ctgcatagct atgcatgaaa
     2881 gtgaatggcg atcggtttgg gcgcatacgg tgcccatgag acacattttc ggacattttc
     2941 agttcgtgaa tccctgactc attgctacat atctgaacag acacccctgg cagcgccccc
     3001 aggggcgtcc tgtaaaaaaa cacaccgcgc cgctagcagc acccctaata taaaatgatg
     3061 ttttttatga aaatagtcag taccacccct aaaaagcggt gttggcgcag ctacaacacc
     3121 gcgccgacac cgctttttta aatatcacaa agagagcaag aagaactaat ttgtcataac
     3181 tctctattta taaagaaaaa tcagcaaaaa attgtttttg cgtggggtgt gggcatttca
     3241 ggcatgagaa cagactc
//
