LOCUS       CP099171                7198 bp    DNA     circular BCT 13-APR-2023
DEFINITION  Escherichia coli strain RHB23-SO-C06 plasmid unnamed2, complete
            sequence.
ACCESSION   CP099171
VERSION     CP099171.1
DBLINK      BioProject: PRJNA605147
            BioSample: SAMN15488586
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7198)
  AUTHORS   Matlock,W.
  TITLE     Plasmid overlap and evolution between Enterobacterales isolates from
            bloodstream infections and non-human compartments
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7198)
  AUTHORS   Matlock,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUN-2022) Nuffield Department of Medicine, University
            of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Assembly Name                     :: RHB23-SO-C06
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 124x
            Sequencing Technology             :: Illumina/Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/15/2022 18:26:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated :: Gene; CDS; rRNA; tRNA; ncRNA; repeat_region
            Genes (total)                     :: 4,720
            CDSs (total)                      :: 4,604
            Genes (coding)                    :: 4,497
            CDSs (with protein)               :: 4,497
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 107
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 47 of 107
            Pseudo Genes (incomplete)         :: 53 of 107
            Pseudo Genes (internal stop)      :: 32 of 107
            Pseudo Genes (multiple problems)  :: 24 of 107
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RHB23-SO-C06"
                     /isolation_source="Pooled soil sample collected from floor
                     of sheep farm"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
     gene            complement(join(6998..7198,1..33))
                     /locus_tag="NFK22_23535"
     CDS             complement(join(6998..7198,1..33))
                     /locus_tag="NFK22_23535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WFY11155.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSRTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(40..558)
                     /locus_tag="NFK22_23540"
     CDS             complement(40..558)
                     /locus_tag="NFK22_23540"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WFY11156.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKVLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMRQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(596..1027)
                     /locus_tag="NFK22_23545"
                     /pseudo=""
     CDS             complement(596..1027)
                     /locus_tag="NFK22_23545"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1236..1559)
                     /locus_tag="NFK22_23550"
     CDS             complement(1236..1559)
                     /locus_tag="NFK22_23550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WFY11157.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPLARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1624..1815
                     /locus_tag="NFK22_23555"
     CDS             1624..1815
                     /locus_tag="NFK22_23555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WFY11158.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2485..2598
                     /locus_tag="NFK22_23560"
     CDS             2485..2598
                     /locus_tag="NFK22_23560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WFY11159.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDKAPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(2816..2956)
                     /gene="crl"
                     /locus_tag="NFK22_23565"
     CDS             complement(2816..2956)
                     /gene="crl"
                     /locus_tag="NFK22_23565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin R system lysis protein"
                     /protein_id="WFY11160.1"
                     /translation="MKMILSGGMLVLMALLSGCQANYIRDVQGGTVAPSSSSELTGIAV
                     Q"
                     /name="crl"
     gene            3076..3600
                     /gene="cri"
                     /locus_tag="NFK22_23570"
     CDS             3076..3600
                     /gene="cri"
                     /locus_tag="NFK22_23570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_089619593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin R immunity protein"
                     /protein_id="WFY11161.1"
                     /translation="MQKEYTYNKDLNRMLFLITFAGIIPLIIIFITYTSNPNFYLIRII
                     FDNTQNIPSVISSYNPVMTKVMDIYGKSAPLLAFIAFIMLFKHTRTPKITDRKKIITAC
                     ILSPFLCIFYAYFFLWHNFELTTSGRPVRLMSENDLTLLIFYICLYYCSFFITYALCLQ
                     PVIAYKLWKER"
                     /name="cri"
     gene            complement(3628..5517)
                     /gene="cra"
                     /locus_tag="NFK22_23575"
     CDS             complement(3628..5517)
                     /gene="cra"
                     /locus_tag="NFK22_23575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin R"
                     /protein_id="WFY11152.1"
                     /translation="MVCFKSQRGMNMPGFNYGGYGDGTGWSSESGGPAPGGGMHGNSGG
                     QRGDNANSSNSVSQQISAIQNDQKLKQKVVNMLIAARKMNPEAKMILGSIAPSGVMQVT
                     IEGVTSTQARQLGLGGLVMGYNASGVIGAVGEIDTGHRLNASGASTPGSETSVESFVNG
                     QKPAGEWHAVAKDSWTGAGPVNVGLVNNAIKSVRIIKKGYVTGVLTPEEVMNKAEYKAM
                     RQAFDSLPLAKQGEAVRQIVAAWSLAYQDFPVNLKKEMGRVTERIVDAINLALILNQTE
                     SRLSESQKNVDVANQIISDTVKAINDVNKKIAEKRNQQVSLTDLMNKKQKEVEDLKKIF
                     KNHSYHRIRDAQREYDDARNKYALLASDINALQAQVSGLTARKQQAEQNKAAAEKAKAD
                     AAAKAAAEKAAAEAKAKAEAEKARKEAEEKANDEKAVLTKASEIIISVGDKAGEYLGDK
                     YKVLSREIADNIKNFQGKTIRSYDEAMASVNKLMANPDLKINAADRDAIVNAWKAFDAE
                     DMGNKFAALGKTFKAADYVMKANNVREKSIEGYQTGNWGPLMLEIESWVLSGIASAVAL
                     SFFSVAVGSLFITAGVSVTAVGLMGIIFAGFIGALIDDDFVDKLNNEIIRPAY"
                     /name="cra"
     gene            5743..6180
                     /locus_tag="NFK22_23580"
     CDS             5743..6180
                     /locus_tag="NFK22_23580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WFY11153.1"
                     /translation="MGHCNFMAWHTLQDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRR
                     EFETSELIRAYGELKQNETQERHTVGQAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRHQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="entry exclusion protein 1"
     gene            6261..6680
                     /locus_tag="NFK22_23585"
     CDS             6261..6680
                     /locus_tag="NFK22_23585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WFY11154.1"
                     /translation="MRLTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSKLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     Typing          complement(1901..1988)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.629"
                     /coverage="100.0"
                     /evalue="6.91e-34"
     Typing          2008..2557
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.928"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3..1246)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.627"
                     /coverage="98.0"
                     /evalue="0.0"
ORIGIN
        1 ggcgttcagc aaatgtgttt ccagctctgt cattctgtgt cactccttca gatgtaccca
       61 ctctttcccc tgaaagggaa tcacctctgc tgatttcccg tacggaagga ccagaaattt
      121 cctgttcccg tcctgcacga actccacgcc ccatgtcttc gcgttcagtt tctgcaatgt
      181 ctcttcctgc ttcctgatat cttccaggtt cgcctgtatc ctcccgccaa gataccagag
      241 cgtcccgcca ctcgcggtaa acaggagaaa gactatcccc agtaacatca tgcccgtatt
      301 ccctgccagc tttaacacgt ccttcctgtg ctgcctcatc gcctctttca ccccttcccg
      361 gtgtttctcc agcgattcct ctgtcgaggc tgtgaacagg gctatagcgt ctctgatttt
      421 cgtctcgttt gatgtcacag cctcgcttac agattcgccg agcctcctga actcgttgtt
      481 cagcactttc tctgtagatt cggctctctc tttcagcttt ttctcgaact ccgcgcccgt
      541 ctgcaaaaga ttgctcataa aatgctcctt tcagcctgat attcttcccg ccgttcgggt
      601 ctgcaatgct gatgctgctt cgcgtcaccc tgacaacttc aagccccgcc tctgtgagcg
      661 cctgaatcac atcctgacgg ccttttatct cccctacatg ataaagagcg tctatcccgc
      721 gtgtgacgct ctcagcaagc gcctgtttcg tttccggcag gttatcagag agagtcagta
      781 tccggcggtt ctccggggcg ttggggtcat gcagcccgta atggtgattc accagcgtct
      841 gccaggcatc aattctcggc ctgtctgccc ggtcgtagta cggctggaga cgttttccgg
      901 tctgtagctc catgttcgga atgacaaaat tcagctcaag ccgtcccttg tcctggtgct
      961 ccacccacag gatgctgtac tgattctttt cgagacctgg catcagtacg cgctcaaagc
     1021 tcgccatcac tttttcacgt cctcccggcg gcagctcctt ctccgcgaac gacagaacgc
     1081 ctgacgtgta tttcttcgca aatggcgtgg catcgatgag ttcccggact tcttccggat
     1141 taccctgaag caccgttgcg ccttcgcggt tacgctccct ccccagcagg taatcaaccg
     1201 gaccactgcc accacctttt cccctggcat gaaatttaac tatcatcccg cgccccctgt
     1261 tccctgacgg ccagacgcag ccggcgcagc tcatccccga tggccatcag tgcggccacc
     1321 acctgaaccc gatcaccgga agaccactgc ccgctgttca ccttacgggc tgtctgattc
     1381 aggttatttc cgatggcggc cagctgacgc agtaacggcg gtgccagtgt cggcagtttc
     1441 ccggaacggg caagcggctc ccccaggcag acccgccgca tccataccgc cagttgttta
     1501 ccctcacagc gttcaagtaa ccgggcatgt tcatcatcag taacccgtat tgtgagcatc
     1561 ctctcgcgtt tcatcggtat cattacccca tgaacagaaa tcccccctta cacggaggca
     1621 tcagtgacta aacaggaaaa aaccgccctt aacatggcgc gctttatcag aagccagaca
     1681 ttaacgctgc tggaaaagct caacgaactg gacgcggatg aacaggcaga catctgtgaa
     1741 tcgcttcacg accacgccga tgagctttac cgcagctgcc tcgcacgttt cggggatgac
     1801 ggtgaaaacc tctgacacat gcagctcccg gagacggaca cagcttgtct gtgagcggat
     1861 gccgggagcc gacaagcccg tcagggcgcg tcagcaggtt ttagcgggtg tcggggcgca
     1921 gccctgaccc agtcacgtag cgatagcgga gtgtatactg gcttagatat gcagcatcag
     1981 tgcggattgt aagaaaacag cgccatatac ggtgtgaaat accgcacaga tgcgtaagga
     2041 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgcta cgctcggtcg
     2101 ttcggctgcg gcgagcggtg caagctcact caaaggcagt aatacggtta tccacagaat
     2161 caggggataa ggccggaaag aacatgtgag caaaaaggca aagggcaacg aaaggcctcg
     2221 ccgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     2281 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     2341 tccctcgtgc gctctcctgt tccgaccctg ccgtttaccg gatacctgtc cgcctttctc
     2401 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgtt ggtatctcag ttcggtgtag
     2461 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     2521 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacaaagc gccactggca
     2581 gcagccactg gtaactgatt tagaggagtt ggtcttgaag ttatgagccg gttaaggcta
     2641 aactgaaaag acaggttttg gtgactgtgc tcctccaagc cagttacctt cggaaaaaga
     2701 gttggtagct cttgatccgg caaacaaacc accgttggta gcggtggttt ttttgtttgc
     2761 aagcagaaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctact
     2821 gaaccgcgat tcccgtcagt tcagaagagg aggatggtgc gacggtccct ccctgaacat
     2881 cgcgaatata attagcctgg cagcctgata gcaaagccat caagacaagc atccctcctg
     2941 ataatatcat tttcatatat cccctcatta actcataccc ctaatattag ggtgaccatt
     3001 actcgcacag catgatatgc ttatacacat caacaactaa attaataaca atgaaaaacc
     3061 gggagtatta taccaatgca aaaagagtac acttacaaca aagatttaaa caggatgctt
     3121 tttttgatta ctttcgcggg tataatccca ttaattatta tttttataac ttatacatca
     3181 aaccccaact tctatttaat ccgcatcatt ttcgataata ctcaaaatat accttcagta
     3241 atttcttcat ataaccctgt gatgacaaag gtgatggata tctacggtaa gagcgcacca
     3301 ttattagcat ttatagcttt catcatgcta ttcaaacaca caagaacccc caaaataaca
     3361 gacagaaaaa aaatcataac agcttgtata ctgtccccct ttttgtgtat tttttatgct
     3421 tattttttct tgtggcataa cttcgagtta acaacatcgg gaagacctgt tcgattgatg
     3481 tctgagaacg atcttacttt gcttatcttt tatatctgcc tgtattattg ctcttttttc
     3541 ataacttacg ccctctgcct ccaaccagta atagcatata aactctggaa agaaaggtag
     3601 tcctcctgac tacctttcga ctctcaatta atacgcaggg cgaataattt cattatttaa
     3661 cttatcaaca aaatcatcat caatcagcgc accgataaag ccagcaaaaa taatgcccat
     3721 aagtcccaca gccgttacag acacgcccgc tgtaataaat aatgaaccaa cagcgactga
     3781 gaaaaatgat agcgccacag cagaagcaat accactcaat acccaggact caatctccag
     3841 catcagcggc ccccagtttc cggtctgata cccctcgata ctcttctccc ggacgttgtt
     3901 tgccttcatc acataatctg cggctttaaa cgtcttaccc agcgcggcaa acttattccc
     3961 catatcctct gcatcaaatg ctttccaggc attcacaatt gcatccctgt cggcggcatt
     4021 aattttaaga tcaggattag ccatcagttt attgacagaa gccatcgcct catcataact
     4081 acgaatcgtt ttgccctgaa agtttttgat attatctgct atctcacgag aaagaacttt
     4141 atatttatca cccagatact ccccagcctt atcaccaaca ctgatgataa tttcactggc
     4201 ttttgtcaga acagccttct cgtcatttgc tttttcctct gcttcctttc tggctttttc
     4261 agcttcagcc tttgctttcg cttcagcagc cgctttttct gctgcagctt tcgccgctgc
     4321 atctgccttt gctttttctg ctgctgcctt gttctgttct gcctgctgtt tccttgccgt
     4381 cagtccggaa acctgtgcct gcagcgcatt aatatcactg gccagtaaag catatttatt
     4441 tcgggcatca tcatattccc tctgggcatc acgaatacgg tgataagagt gatttttgaa
     4501 tatcttttta agatcttcga cctctttttg ttttttattc atcaaatcag tcagtgatac
     4561 ctgctgattt cgtttttctg ctattttctt atttacatca ttaatagctt ttactgtatc
     4621 actgataatc tggttcgcta catcaacatt cttttgtgat tccgacaaac gggactctgt
     4681 ctgattaaga atgagcgcca ggttaatagc atcaacaatc cgttcagtga cacgccccat
     4741 ctcttttttc agatttaccg gaaaatcctg atatgccaga ctccaagcag caacaatctg
     4801 tctgacagcc tctccctgtt ttgccagtgg aagggaatcg aaagcctgcc gcattgcttt
     4861 gtactctgct ttattcatca cctcttctgg cgtgagaacg cccgtcacat agcctttttt
     4921 gataatccgg acacttttga tggcgttatt aaccagccct acattcactg gaccagcccc
     4981 tgtccagctg tccttcgcca cagcatgcca ttctccggct ggtttctgtc cgttcacaaa
     5041 actctccaca gaagtttcgc ttccgggagt acttgctcct gatgcattaa ggcggtgacc
     5101 ggtgtctatt tcgcccacag ccccaataac accagaagcg ttatatccca tgaccagccc
     5161 tcccaaacca agctgcctgg cctgagtgga ggttaccccc tcaatggtga cctgcataac
     5221 accagaagga gcaatacttc ccagaatcat ttttgcttca ggattcattt ttctcgcagc
     5281 aatcagcata ttcaccactt tctgtttaag tttctgatca ttctgaatgg cagaaatctg
     5341 ctgactcaca gaattgctac tgtttgcgtt atctccgcgc tggcctccac tatttccatg
     5401 cataccaccg cctggagctg gaccaccact ttcagaactc cagccagtac cgtcaccata
     5461 cccaccgtaa ttaaaccccg gcatattcat tcctctttga cttttaaaac aaaccattaa
     5521 aaacaaatac tgtacacata accactggct ttatgtacag tataaaagct acgccgctgc
     5581 attttccctg tcaagagtgt ggacttccat ttttgtgaaa acgatcaaaa aaacagtctc
     5641 tcacaccacg cgctattccc gccggaaacc acaaaaaccg gcgcaaacat taccgttctc
     5701 agacctcatt atgttttact gaaactatga gatgagacac ctatgggaca ctgtaacttt
     5761 atggcatggc acacactcca ggacgcactg aaaatgactg gaagatcgcg ttcacagttt
     5821 taccgtgata tgcgctcagg tcttgtcagt taccgcacca gccaggacgg acgaagggag
     5881 tttgaaacca gtgaactgat ccgggcatac ggcgaattaa agcaaaatga gacacaagaa
     5941 aggcacactg tgggacaggc agaaaatcca catgatcagc agacagaacg cattctccgg
     6001 gaactgaatg agctgaaaca atgcctgacg ctgatgcttg aggataaaca ggcacaggat
     6061 atggatcgca gacaccagga agcagaacgg gaacagctac aaaatgagat agcccagctc
     6121 aggcaggcac tggaactgga aaagaaacgg ggattctggt ccaggttgtt cggtcgctga
     6181 acgctgtcag agactgatga taaaatagtc ttcggataat aactcaccga gaataaatac
     6241 tttaaggtag ggagacactc atgagactta ccggaaacaa actttgtctt atcgccatga
     6301 taacagcaac agtagctctc acagcctgta ccccaaaggg cagcgtggaa caacataccc
     6361 ggcattacgt atatgcttct gatgacggtt ttgatcccaa cttttccacc caaaaagccg
     6421 acacaacacg aatgatggtg cctttttttc ggcagttctg ggatatggga gctaaagata
     6481 aagcgacagg aaaatcacgg agtgatgtgc aacaacgcat tcagcagttt cacagccaag
     6541 aatttttaaa ctcactccgg ggcacaactc aatttgcggg tactgattac cgcagcaaag
     6601 accttacccc gaaaaaatcc aagctgctgg ctgacacgat ttctgcggtt tatctcgatg
     6661 gctacgaggg aagacagtaa gtggatttac cataatccct taattgtacg caccgccaaa
     6721 acgcgttcag cgcgatcacg gcagcagaca ggtaaaaatg gcaacaaacc acccgaaaaa
     6781 ctgccgcgat cgcgcctgat aaattttaac cgcatgaata cctatgcaac cagatggtac
     6841 agaccacatt agcccgtctt aatccactta acctgccatt tttcatagtt tcaccatccc
     6901 agcgaaggac catccagtgt gcgttcctgt atttccagct ggcgctcacg ctctagggat
     6961 aacgcatgtt cgcgctcctg tatcagccgt tcctctctta tctccagttc tcgctgtata
     7021 actggctcaa gcgttctgtc tgctcgctca agtgctgcac ctgctgactc aactgcatga
     7081 cccgctcgtt cagcatcgcg ttgtcccgtt gcgtacgcga aaacatcttc tgcaattcca
     7141 cgaaggcgct ctcccattcg ctcagccgct gcatatagtc ctgttgcagc tgctctaa
//
